FastQCFastQC Report
Fri 10 Feb 2017
SRR3224562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972031
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT14470.14886356505090886No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG12300.12653917416214092No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG12080.12427587186005384No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG11520.11851473872746858No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA11080.11398813412329442No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT10470.10771261410387119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14900.048.997661
GGTATCA7600.048.9723781
AAACCGT1850.038.504116
TATCACG500.00161763337.990722
ATCAACG19100.037.5431863
AAATGCG2550.037.2458046
TCAACGC19400.037.2074054
CCGTGCG1750.035.27719
AATGCGG2750.034.5370187
AACCGTG2150.033.131447
TATCAAC24450.030.105122
CAACGCA24800.029.1057935
ATGCGGG2950.028.9759738
ACGCAGA26000.027.9450977
AACGCAG25850.027.9235486
CGCAGAG26100.027.8380288
ACCGTGC2701.8189894E-1224.6236158
GTACATG45050.024.3614541
TACATGG45200.023.8492622
AGACCGC6650.023.5656726
Produced by FastQC (version 0.11.5)