##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	972031
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.182223612209896	34.0	33.0	34.0	31.0	34.0
2	33.309657819555134	34.0	34.0	34.0	31.0	34.0
3	33.421554456596546	34.0	34.0	34.0	31.0	34.0
4	36.625142613764375	37.0	37.0	37.0	35.0	37.0
5	36.6608266608781	37.0	37.0	37.0	35.0	37.0
6	36.70184901510343	37.0	37.0	37.0	36.0	37.0
7	36.68333623104613	37.0	37.0	37.0	36.0	37.0
8	36.66797766737892	37.0	37.0	37.0	35.0	37.0
9	38.517863113419224	39.0	39.0	39.0	37.0	39.0
10-11	38.53916644633762	39.0	39.0	39.0	37.5	39.0
12-13	38.536254502171225	39.0	39.0	39.0	37.0	39.0
14-15	40.15075650879447	41.0	40.0	41.0	38.0	41.0
16-17	40.155881859734926	41.0	40.0	41.0	38.0	41.0
18-19	40.148994219320166	41.0	40.0	41.0	38.0	41.0
20-21	40.15099106921487	41.0	40.0	41.0	38.0	41.0
22-23	40.078818473896405	41.0	40.0	41.0	38.0	41.0
24-25	40.043507357275644	41.0	40.0	41.0	38.0	41.0
26-27	39.99022562037631	41.0	40.0	41.0	38.0	41.0
28-29	39.966599316276955	41.0	40.0	41.0	38.0	41.0
30-31	39.9214351188388	41.0	40.0	41.0	38.0	41.0
32-33	39.89057087685475	41.0	40.0	41.0	38.0	41.0
34-35	39.815903505135125	41.0	40.0	41.0	38.0	41.0
36-37	39.72508541394256	41.0	40.0	41.0	37.0	41.0
38-39	39.63754551037981	41.0	40.0	41.0	37.0	41.0
40-41	39.52710973209702	41.0	40.0	41.0	37.0	41.0
42-43	39.44474764693719	41.0	39.0	41.0	36.0	41.0
44-45	39.23468438763784	41.0	39.0	41.0	35.0	41.0
46-47	39.04029964064932	41.0	39.0	41.0	35.0	41.0
48-49	38.965065414580394	40.5	39.0	41.0	35.0	41.0
50-51	38.820467145595146	40.0	38.0	41.0	35.0	41.0
52-53	38.59645422831164	40.0	37.5	41.0	35.0	41.0
54-55	38.42266656104589	40.0	37.0	41.0	35.0	41.0
56-57	38.209546300478074	40.0	36.5	41.0	35.0	41.0
58-59	37.94913433830814	40.0	36.0	41.0	34.0	41.0
60-61	37.69973231306409	39.0	35.0	41.0	34.0	41.0
62-63	37.4805181110479	39.0	35.0	41.0	34.0	41.0
64-65	37.20424400044854	38.5	35.0	41.0	34.0	41.0
66-67	36.90332047023192	37.5	35.0	40.5	33.0	41.0
68-69	36.61193470167103	37.0	35.0	40.0	33.0	41.0
70-71	36.28654127286064	36.5	35.0	39.0	33.0	41.0
72-73	35.94549402230999	36.0	35.0	39.0	33.0	41.0
74-75	35.601830085665995	35.5	35.0	39.0	33.0	40.5
76-77	33.73020459224037	34.5	32.5	36.5	29.5	39.0
78-79	34.98026811902089	35.0	35.0	37.0	32.5	39.0
80-81	34.86885809197443	35.0	35.0	36.5	33.0	39.0
82-83	34.65029201743566	35.0	35.0	36.0	33.0	37.0
84-85	34.44445187447725	35.0	35.0	36.0	33.0	37.0
86-87	34.24440115593021	35.0	35.0	35.5	32.5	36.5
88-89	34.10025040353651	35.0	35.0	35.0	32.0	36.0
90-91	33.96922268939983	35.0	35.0	35.0	32.0	36.0
92-93	33.88270487258123	35.0	35.0	35.0	32.0	36.0
94-95	33.79695297783712	35.0	34.0	35.0	32.0	36.0
96-97	33.749814563527295	35.0	34.0	35.0	32.0	35.0
98-99	33.72739398229069	35.0	34.0	35.0	32.0	35.0
100-101	32.902037589336146	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	12.0
15	32.0
16	84.0
17	168.0
18	270.0
19	447.0
20	646.0
21	952.0
22	1319.0
23	1467.0
24	1649.0
25	2247.0
26	3114.0
27	4316.0
28	5829.0
29	6640.0
30	6909.0
31	7662.0
32	9230.0
33	12599.0
34	21012.0
35	42169.0
36	115937.0
37	262422.0
38	366236.0
39	98641.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.912908536755516	18.638980785866437	13.70827300171874	24.739837675659306
2	15.935397122108247	20.993569135140753	38.03407504493169	25.036958697819305
3	19.433125075229082	26.38218328427797	28.626864781061514	25.557826859431437
4	13.351631789521115	18.299725008770295	38.82828839820952	29.520354803499067
5	14.798396347441594	37.22000635782192	33.931119480757296	14.050477813979183
6	28.998149235981156	36.47219070173688	20.578870426971978	13.950789635309985
7	27.58091048536518	31.102094480525828	22.325111030409523	18.99188400369947
8	26.21552193294247	33.016951105468856	21.42369944991466	19.34382751167401
9	27.55477963151381	15.655776410423124	19.951935689293858	36.837508268769206
10-11	25.90508944673575	24.979450243870822	27.908883564413067	21.206576744980353
12-13	26.950837987677346	22.8347141191999	27.40833368483104	22.80611420829171
14-15	24.10607274870863	24.350355081267985	25.07368592153954	26.469886248483842
16-17	23.68772189364331	26.7071729193822	25.895213218508463	23.709891968466028
18-19	23.644976343347075	26.401832863355178	26.75459939034866	23.198591402949084
20-21	24.870939135122455	25.24809109258844	26.186855853056823	23.694113919232283
22-23	24.989969321555776	25.642477814196855	25.54109080284521	23.826462061402154
24-25	24.453320507907442	25.435288610661733	25.9309021606899	24.180488720740925
26-27	24.01771793382678	26.463218723685312	26.00792481966483	23.51113852282308
28-29	23.795073968659448	26.35140244413706	26.34234894153752	23.51117464566597
30-31	24.230225360241516	25.71728094491737	26.12900023301954	23.923493461821575
32-33	23.954855326041724	25.734171921560073	26.63635554645662	23.674617205941583
34-35	24.498915628935215	25.78740360002963	26.036531164865558	23.677149606169596
36-37	23.55566712224972	26.010002937198983	26.33901037484523	24.095319565706067
38-39	23.787307194935142	25.886314325366165	26.00776106934861	24.318617410350083
40-41	24.029017593060303	25.733747174730027	26.348388065812717	23.888847166396957
42-43	24.036476202919456	25.757306094147204	26.592567520994702	23.61365018193864
44-45	24.205022524040302	25.722160805752083	26.158289191495754	23.914527478711864
46-47	24.05325698130477	25.47492228123306	25.954130098049966	24.517690639412205
48-49	23.74721650820889	25.432254606489234	26.43099383709584	24.38953504820604
50-51	23.87007204502737	25.748870149203064	26.1741652272407	24.206892578528873
52-53	24.194199667603023	25.705829189516173	25.824292550490956	24.27567859238985
54-55	23.993267704425065	25.329027572165906	26.31418133783799	24.363523385571035
56-57	24.101133190527047	25.568395775073927	25.975208499237613	24.35526253516142
58-59	24.453336585551607	25.647754914477467	26.02628842133105	23.872620078639873
60-61	23.977837385313666	25.953599083692787	26.04683519488251	24.021728336111035
62-63	23.740652304298937	25.55592362795014	26.448179121859283	24.255244945891643
64-65	24.06661927448816	25.540440582656316	26.06244039541949	24.330499747436036
66-67	23.7071142792771	25.80216063067947	25.91686890644434	24.573856183599084
68-69	23.974749776498896	25.828034291087427	26.292319895147376	23.9048960372663
70-71	24.230554375323422	25.621868026842765	26.09875610963025	24.048821488203565
72-73	23.95098510232698	25.39924138221929	26.2912911213737	24.358482394080024
74-75	23.940697364590225	25.231499818421426	26.449722282519794	24.378080534468552
76-77	23.83042266764812	24.99530620758548	25.97392977675037	25.200341348016032
78-79	23.908874382044438	24.905422576148073	26.13726972956567	25.048433312241826
80-81	23.46207147729412	25.580700286942125	26.247899890223287	24.709328345540463
82-83	23.576638994096324	25.590431898882894	26.326320971295385	24.506608135725394
84-85	23.657024633967808	24.9778937800633	26.562751572383657	24.80233001358523
86-87	23.537315167931887	25.676598791602324	26.112490239508823	24.673595800956967
88-89	23.85731525023379	25.251046520121275	25.81049369824625	25.081144531398692
90-91	23.769612286027915	25.356187199790952	25.826542569115595	25.047657945065538
92-93	23.73741166691186	25.142408009621093	26.375239061305656	24.74494126216139
94-95	23.589268243502524	25.082276182549734	26.203433841101777	25.12502173284597
96-97	23.62244619770357	25.62500578685248	26.057347965239792	24.69520005020416
98-99	22.719748649991615	25.589461652971973	26.553782749727116	25.137006947309292
100-101	23.362808536090093	25.162410443270744	26.23217551497532	25.24260550566384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	1.0
4	0.0
5	1.5
6	3.0
7	4.5
8	7.0
9	10.5
10	13.0
11	10.5
12	12.5
13	18.0
14	20.0
15	23.5
16	26.0
17	30.0
18	33.0
19	35.0
20	43.0
21	49.0
22	64.5
23	95.0
24	117.0
25	143.0
26	194.5
27	256.0
28	317.5
29	392.5
30	493.0
31	639.5
32	903.0
33	1450.5
34	2586.0
35	4819.5
36	9503.5
37	16635.5
38	25996.0
39	35279.0
40	42254.5
41	46482.0
42	46545.0
43	44863.0
44	42639.5
45	40985.5
46	39956.0
47	39541.5
48	40718.5
49	43306.5
50	46403.5
51	45259.5
52	42272.5
53	41207.5
54	41407.0
55	40407.5
56	34611.0
57	28977.5
58	26861.0
59	25067.5
60	20281.5
61	14857.0
62	11490.0
63	8021.5
64	5007.0
65	3275.5
66	2562.0
67	2079.5
68	1498.5
69	974.5
70	596.5
71	398.0
72	295.0
73	216.5
74	148.5
75	114.5
76	77.5
77	47.0
78	30.0
79	19.5
80	12.0
81	8.5
82	9.5
83	7.5
84	3.0
85	3.0
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4590388578142055
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.258963963083483E-4
22-23	0.001337405905778725
24-25	3.6007082078657986E-4
26-27	0.002211863613403276
28-29	0.0032406373870792187
30-31	9.773350849921454E-4
32-33	5.143868868379713E-5
34-35	0.004012217717336176
36-37	0.0016974767265653052
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0071499777270478005
46-47	0.07618069794070353
48-49	5.658255755217683E-4
50-51	0.0
52-53	5.143868868379713E-5
54-55	0.0
56-57	0.008744577076245512
58-59	0.039762106352575176
60-61	0.031326161408432446
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.5719344341898563E-4
78-79	0.007818680679937164
80-81	0.005966887887320466
82-83	9.773350849921454E-4
84-85	0.002006108858668088
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06681885660025245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	972031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.26191740008082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13274335760465	40.768467141118144
2	14.628509941965293	15.875419963143633
3	4.760179256173011	7.748893608241142
4	1.99107059017169	4.3215723160650565
5	1.060870436690224	2.8782432003936296
6	0.5896436937426931	1.9197118443206738
7	0.3784219915877273	1.4373731994935137
8	0.25105217112575434	1.089805773818931
9	0.17639470755489756	0.8614363546039736
>10	0.8666768273240484	8.410015308285448
>50	0.07699561617915722	2.9533632092085678
>100	0.0804080792553543	8.652855849185274
>500	0.00589279052407332	2.341012690719993
>1k	0.0011405401014335458	0.7418295414019294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1447	0.14886356505090886	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1230	0.12653917416214092	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1208	0.12427587186005384	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1152	0.11851473872746858	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1108	0.11398813412329442	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1047	0.10771261410387119	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0287737736759424E-4	0.0
8	0.0	0.0	0.0	1.0287737736759424E-4	0.0
9	0.0	0.0	0.0	1.0287737736759424E-4	0.0
10-11	0.0	0.0	0.0	1.0287737736759424E-4	0.0
12-13	0.0	0.0	0.0	1.0287737736759424E-4	0.0
14-15	0.0	0.0	0.0	1.0287737736759424E-4	0.0
16-17	0.0	0.0	0.0	1.0287737736759424E-4	0.0
18-19	0.0	0.0	0.0	1.0287737736759424E-4	0.0
20-21	0.0	0.0	0.0	2.5719344341898563E-4	0.0
22-23	0.0	0.0	0.0	4.629481981541741E-4	0.0
24-25	0.0	0.0	0.0	5.143868868379713E-4	0.0
26-27	0.0	0.0	0.0	9.773350849921454E-4	0.0
28-29	0.0	0.0	0.0	0.0018517927926166963	0.0
30-31	0.0	0.0	0.0	0.006018326576004263	0.0
32-33	0.0	0.0	0.0	0.018877998746953544	0.0
34-35	0.0	0.0	0.0	0.0338980958426223	0.0
36-37	0.0	0.0	0.0	0.05879442116558011	0.0
38-39	0.0	0.0	0.0	0.10730110459440079	1.0287737736759424E-4
40-41	0.0	0.0	0.0	0.1660440870712971	1.0287737736759424E-4
42-43	0.0	0.0	0.0	0.22648454627475872	1.0287737736759424E-4
44-45	0.0	0.0	0.0	0.2965954789507742	1.0287737736759424E-4
46-47	0.0	0.0	0.0	0.3783315552693278	1.0287737736759424E-4
48-49	0.0	0.0	0.0	0.4686064539093918	2.0575475473518849E-4
50-51	0.0	0.0	0.0	0.5654655047009818	2.0575475473518849E-4
52-53	0.0	0.0	0.0	0.6672626696062163	2.0575475473518849E-4
54-55	0.0	0.0	0.0	0.7658191971243715	2.0575475473518849E-4
56-57	0.0	0.0	0.0	0.8833051620781642	2.0575475473518849E-4
58-59	0.0	0.0	0.0	1.0142166247784279	2.0575475473518849E-4
60-61	0.0	0.0	0.0	1.1653434921314239	2.0575475473518849E-4
62-63	0.0	0.0	0.0	1.3134354768520757	2.0575475473518849E-4
64-65	0.0	0.0	0.0	1.4585954563177512	2.0575475473518849E-4
66-67	0.0	0.0	0.0	1.6314294502953095	2.5719344341898563E-4
68-69	0.0	0.0	0.0	1.8201065603874773	3.0863213210278274E-4
70-71	0.0	0.0	0.0	2.036457684991528	3.0863213210278274E-4
72-73	0.0	0.0	0.0	2.26407388241733	3.0863213210278274E-4
74-75	0.0	0.0	0.0	2.4924102214847057	3.0863213210278274E-4
76-77	0.0	0.0	0.0	2.733400477968295	3.0863213210278274E-4
78-79	0.0	0.0	0.0	3.0079801981624046	3.0863213210278274E-4
80-81	0.0	0.0	0.0	3.31774398141623	3.0863213210278274E-4
82-83	0.0	0.0	0.0	3.6461800086622755	4.1150950947037697E-4
84-85	0.0	0.0	0.0	3.98058292379564	4.1150950947037697E-4
86-87	0.0	0.0	0.0	4.333400889477804	4.1150950947037697E-4
88-89	0.0	0.0	0.0	4.71847091296471	4.1150950947037697E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1490	0.0	48.99766	1
GGTATCA	760	0.0	48.972378	1
AAACCGT	185	0.0	38.50411	6
TATCACG	50	0.001617633	37.99072	2
ATCAACG	1910	0.0	37.543186	3
AAATGCG	255	0.0	37.245804	6
TCAACGC	1940	0.0	37.207405	4
CCGTGCG	175	0.0	35.2771	9
AATGCGG	275	0.0	34.537018	7
AACCGTG	215	0.0	33.13144	7
TATCAAC	2445	0.0	30.10512	2
CAACGCA	2480	0.0	29.105793	5
ATGCGGG	295	0.0	28.975973	8
ACGCAGA	2600	0.0	27.945097	7
AACGCAG	2585	0.0	27.923548	6
CGCAGAG	2610	0.0	27.838028	8
ACCGTGC	270	1.8189894E-12	24.623615	8
GTACATG	4505	0.0	24.361454	1
TACATGG	4520	0.0	23.849262	2
AGACCGC	665	0.0	23.565672	6
>>END_MODULE