FastQCFastQC Report
Fri 10 Feb 2017
SRR3224561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001717
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG26240.2619502314525959No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA23320.2328002819159503No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA20130.20095496033310806No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC16100.16072403682876502No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13980.1395603748364059No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA13880.1385620888933701No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT12720.12698197195415473No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG12470.12448625709656519No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA12300.12278917099340432No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA11720.11699911252379665No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC11690.1166996267408859No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA11610.11590099798645727No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11430.11410408328899281No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC11080.11061008248836747No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA10720.10701625309343855No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG10670.10651711012192065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG2550.054.0305149
AAACCGT2750.048.342016
GGTATCA9900.045.637881
AACCGTG3350.041.1010177
GTATCAA16400.040.019561
ACCGTGC3900.038.9588748
GTCTAAT6300.033.216141
TGCGCGG2350.030.34042512-13
ATCAACG21750.030.1244563
CTAATGT7450.029.3157443
GTGCGCG2400.028.71231710-11
TCAACGC22950.028.549324
TCTAATG7700.027.7473282
GTACATG36250.027.5516951
GCGCGGC2450.027.16190512-13
TAACGCA1056.92602E-527.130724
GTTATTA7600.026.8643258
TACATGG37350.026.313532
AATGCGG2409.094947E-1225.7176637
CGCAGAG26050.025.153158
Produced by FastQC (version 0.11.5)