##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224561_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001717
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16515442984396	34.0	31.0	34.0	31.0	34.0
2	32.33267879051668	34.0	31.0	34.0	31.0	34.0
3	32.47533984149216	34.0	31.0	34.0	31.0	34.0
4	35.76629726759155	37.0	37.0	37.0	35.0	37.0
5	35.7750552301698	37.0	37.0	37.0	35.0	37.0
6	35.876592890007856	37.0	37.0	37.0	35.0	37.0
7	35.83460897638754	37.0	37.0	37.0	35.0	37.0
8	35.870443448598756	37.0	37.0	37.0	35.0	37.0
9	37.594631018541165	39.0	39.0	39.0	35.0	39.0
10-11	37.6612491352348	39.0	38.5	39.0	35.0	39.0
12-13	37.657811537589964	39.0	39.0	39.0	35.0	39.0
14-15	39.14132434609775	41.0	40.0	41.0	36.0	41.0
16-17	38.97432907697484	41.0	39.5	41.0	35.5	41.0
18-19	39.05177060986287	41.0	39.5	41.0	36.0	41.0
20-21	39.11376316863945	41.0	40.0	41.0	36.0	41.0
22-23	39.067975286433196	41.0	40.0	41.0	36.0	41.0
24-25	39.00403906492552	41.0	39.0	41.0	35.5	41.0
26-27	38.83307511003607	41.0	39.0	41.0	35.0	41.0
28-29	38.74718458406915	41.0	39.0	41.0	35.0	41.0
30-31	38.66299314077729	41.0	39.0	41.0	35.0	41.0
32-33	38.465592078401386	40.5	39.0	41.0	34.0	41.0
34-35	38.458009098378085	40.0	38.0	41.0	34.0	41.0
36-37	38.44580205786664	40.0	38.0	41.0	34.5	41.0
38-39	38.31870977531578	40.0	38.0	41.0	34.0	41.0
40-41	38.21627665298682	40.0	38.0	41.0	34.0	41.0
42-43	38.05596440910956	40.0	38.0	41.0	33.0	41.0
44-45	37.866345983945564	40.0	38.0	41.0	33.0	41.0
46-47	37.67278033616281	40.0	37.0	41.0	33.0	41.0
48-49	37.445667788407306	40.0	37.0	41.0	32.0	41.0
50-51	36.14843713344188	38.5	35.0	40.0	30.0	40.5
52-53	36.46951434387157	39.0	35.0	40.0	30.5	40.5
54-55	36.91352946990018	39.0	35.0	41.0	31.0	41.0
56-57	36.742868494794436	39.0	35.0	41.0	31.0	41.0
58-59	36.58004456348449	39.0	35.0	41.0	31.0	41.0
60-61	36.31983684014547	38.0	35.0	41.0	31.0	41.0
62-63	36.022653104619366	37.5	35.0	41.0	30.5	41.0
64-65	35.76208799491273	37.0	35.0	40.0	30.0	41.0
66-67	35.51573448389116	36.5	35.0	40.0	30.0	41.0
68-69	35.19955736001286	36.0	35.0	39.5	29.0	41.0
70-71	34.90472508702558	35.0	35.0	39.0	29.0	41.0
72-73	34.590988772277996	35.0	34.0	39.0	29.0	41.0
74-75	34.3125618313356	35.0	34.0	37.5	29.0	40.0
76-77	33.99340682048922	35.0	34.0	37.0	28.5	39.0
78-79	33.77093480493991	35.0	34.0	36.5	28.5	39.0
80-81	33.481126405960964	35.0	34.0	36.0	28.0	38.5
82-83	33.230276115908985	35.0	34.0	36.0	27.0	37.0
84-85	33.01713857307004	35.0	34.0	35.0	27.0	37.0
86-87	32.803392574948816	35.0	33.5	35.0	27.0	36.5
88-89	32.66373137323216	35.0	33.0	35.0	26.5	36.0
90-91	32.49918190466968	35.0	33.0	35.0	26.0	36.0
92-93	32.35993099847562	35.0	33.0	35.0	25.5	36.0
94-95	32.23500349899223	35.0	33.0	35.0	25.0	35.0
96-97	32.11012641294897	35.0	33.0	35.0	25.0	35.0
98-99	32.06468144196415	35.0	33.0	35.0	25.0	35.0
100-101	30.848538559293694	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	105.0
8	379.0
9	398.0
10	504.0
11	1117.0
12	1844.0
13	2178.0
14	2140.0
15	2275.0
16	2406.0
17	2606.0
18	2979.0
19	3514.0
20	4074.0
21	5240.0
22	6142.0
23	5986.0
24	5677.0
25	5877.0
26	6060.0
27	6487.0
28	7249.0
29	8178.0
30	10084.0
31	12749.0
32	16908.0
33	23568.0
34	37754.0
35	69319.0
36	148663.0
37	254971.0
38	267281.0
39	76982.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70536178448889	18.804035734566074	13.145529580190829	24.3450729007542
2	16.21707150529691	19.389327913300775	38.56831676842538	25.825283812976927
3	19.780296571676146	25.32389484423698	28.24119459246851	26.654613991618366
4	12.760090923883691	16.40303598720996	38.30083746207762	32.536035626828735
5	13.847224315849685	38.495303563780986	34.03925459985205	13.618217520517273
6	30.807902830839453	36.83754992677572	18.580696943348272	13.773850299036555
7	27.52683642186366	30.933686859661965	22.17352805233414	19.365948666140238
8	26.351155066750387	34.30070568833313	20.18793731163592	19.160201933280558
9	26.669308796795903	15.151484900425968	20.03569870532296	38.14350759745517
10-11	26.35564735349405	24.758789159013972	27.528084279292454	21.35747920819952
12-13	26.534939508863282	22.480600808411957	27.7120184642968	23.272441218427957
14-15	23.70342555803945	24.494265437181458	24.757262889097163	27.04504611568193
16-17	23.660795904686452	26.988937498314097	25.938784093894895	23.41148250310455
18-19	23.66276532750978	26.486767700067205	26.677141467730326	23.17332550469269
20-21	24.785476735011834	24.811443238222694	26.452676047898212	23.95040397886726
22-23	24.652443571560937	25.86400232961331	25.71970016717873	23.763853931647027
24-25	24.03631819709017	25.53716215035598	26.228749526352757	24.197770126201092
26-27	23.874457556375702	26.519016848071857	26.323602374722597	23.282923220829836
28-29	23.615651925643668	26.611607869288434	26.394630419569598	23.3781097854983
30-31	23.73824143944847	25.669774996331302	26.53803419528669	24.05394936893354
32-33	23.662022307697683	25.798354225794313	26.680339856466446	23.859283610041558
34-35	24.297081910359914	25.57364006001695	26.20874957697633	23.920528452646806
36-37	23.596122123745353	25.616190502817844	26.888262923924287	23.89942444951252
38-39	23.76349566147148	25.898641710872255	26.104249476745956	24.233613150910312
40-41	24.383485605637446	25.45650493006855	26.485896654501857	23.67411280979215
42-43	24.199546363017404	25.77734250989835	26.46962808556009	23.553483041524157
44-45	24.28383086402558	25.429302119166753	26.16068446724804	24.126182549559626
46-47	24.121283755791307	25.481198781691834	25.987729069188205	24.409788393328654
48-49	23.552809825529568	25.810133999922137	26.388441046722775	24.248615127825524
50-51	23.836622519134647	25.96896129345913	25.999159443235964	24.19525674417026
52-53	24.196704258787662	25.952888889776254	25.371037927877833	24.47936892355825
54-55	24.07920600329235	25.316182115308017	25.908465165311164	24.696146716088478
56-57	24.10417518329787	25.870343555287352	25.728487051975286	24.296994209439497
58-59	24.48483934755553	25.857153340825008	26.06208436568481	23.595922945934653
60-61	24.19727976739635	26.101262865290376	25.751798245088526	23.94965912222475
62-63	23.820111283313096	25.677146577618714	26.336168674506293	24.166573464561896
64-65	24.584355423804656	25.210344221426745	25.5909314273843	24.6143689273843
66-67	23.74801118082067	25.699512698738385	25.520537693833422	25.03193842660752
68-69	24.02854299168328	25.836488748818283	26.364981327061436	23.769986932437007
70-71	24.28031070651691	25.465825178169084	26.086160063171533	24.167704052142472
72-73	24.1823788555051	25.670174310708514	26.26200813203729	23.885438701749095
74-75	24.109803467446394	25.504109444084506	26.24493744241138	24.14114964605772
76-77	24.10076897966192	25.120917384850213	25.720388093643216	25.05792554184465
78-79	24.090235353379533	24.739387930444874	26.306579939922308	24.863796776253285
80-81	23.93760319805336	25.698999809211585	25.84703563747804	24.51636135525702
82-83	23.82775922488355	26.021095864277715	25.978047156812657	24.173097754026077
84-85	23.89950923976351	25.282515424570768	26.54349312041925	24.274482215246472
86-87	23.782396384760496	26.28964968876596	25.85692374004278	24.071030186430765
88-89	24.140913160340073	25.514533361095342	25.515981379482177	24.828572099082404
90-91	23.793796052178408	25.365247869408226	25.888050217776076	24.952905860637287
92-93	23.799233989578543	25.528273152148355	26.366611809019513	24.305881049253582
94-95	24.085345461842017	25.37203621382087	26.23755012643291	24.3050681979042
96-97	24.187070799437365	25.827204689548044	26.121848785635066	23.863875725379526
98-99	23.19212986187127	26.143681233250526	26.21785436011702	24.446334544761186
100-101	24.0240841597261	25.407020701351023	25.840733331402394	24.728161807520483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	5.0
3	8.0
4	9.0
5	7.5
6	9.5
7	24.0
8	38.0
9	40.5
10	36.5
11	48.5
12	66.5
13	64.0
14	63.5
15	80.5
16	101.5
17	108.0
18	111.0
19	140.0
20	174.0
21	207.5
22	265.0
23	342.0
24	452.0
25	644.0
26	988.5
27	1481.5
28	2164.5
29	3298.0
30	5136.0
31	7552.0
32	10287.0
33	13273.5
34	16491.0
35	19168.5
36	21553.5
37	23279.5
38	23861.5
39	24380.5
40	25096.0
41	25593.0
42	26039.5
43	26901.5
44	28663.5
45	31727.0
46	35114.5
47	36614.0
48	38479.0
49	42794.5
50	46796.5
51	46330.5
52	44441.5
53	45545.0
54	47372.5
55	46853.5
56	40472.5
57	34316.5
58	31692.0
59	29121.5
60	24304.5
61	18716.0
62	15276.0
63	11272.0
64	7228.0
65	4857.0
66	3751.0
67	3218.0
68	2468.5
69	1537.5
70	913.5
71	610.0
72	401.0
73	309.0
74	241.0
75	179.5
76	137.5
77	91.5
78	66.0
79	48.0
80	25.0
81	20.0
82	18.0
83	11.0
84	10.0
85	10.0
86	7.5
87	9.0
88	9.0
89	9.5
90	7.5
91	3.0
92	3.0
93	1.5
94	2.5
95	4.0
96	5.5
97	4.5
98	2.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14505094752310282
2	9.982859430358075E-5
3	2.9948578291074225E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.037585465755298156
16-17	0.07666836042515002
18-19	0.026554406084752482
20-21	0.042626809767628976
22-23	0.06913130155522967
24-25	0.018019061271796324
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.024058691227162962
38-39	0.00524100120093799
40-41	0.005740144172455893
42-43	0.003494000800625326
44-45	0.012029345613581481
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.9914297151790374E-5
58-59	0.01602248938572471
60-61	0.01846828994616244
62-63	0.030847035639806453
64-65	0.05001412574609396
66-67	0.018019061271796324
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.018019061271796324
80-81	0.060695785336577095
82-83	0.052459926306531686
84-85	0.031096607125565404
86-87	0.06204347135967544
88-89	0.034790265114797894
90-91	0.0
92-93	0.003344257909169955
94-95	0.0
96-97	0.0
98-99	6.48885862973275E-4
100-101	0.0477180680771116
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1001717.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.61664247853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99873663275878	39.46181711747299
2	14.571732004591636	15.334312263571025
3	4.857110810365728	7.666945889628497
4	2.166324093007969	4.559388011777042
5	1.0735883957328216	2.824430839368623
6	0.6161657238225849	1.9452342958738564
7	0.3642235818186433	1.341495539076082
8	0.2420661062753812	1.0189364616049263
9	0.16648450981604843	0.7883870338083904
>10	0.7647207214408407	7.286602421715671
>50	0.08098492285062393	3.015258269645292
>100	0.08596548820566455	9.470381108022918
>500	0.008826813361517213	2.964018546336082
>1k	0.0030701959518320745	2.322792202098576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2624	0.2619502314525959	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2332	0.2328002819159503	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2013	0.20095496033310806	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1610	0.16072403682876502	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1398	0.1395603748364059	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1388	0.1385620888933701	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1272	0.12698197195415473	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1247	0.12448625709656519	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1230	0.12278917099340432	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1172	0.11699911252379665	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1169	0.1166996267408859	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1161	0.11590099798645727	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1143	0.11410408328899281	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1108	0.11061008248836747	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1072	0.10701625309343855	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1067	0.10651711012192065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.982859430358075E-5	0.0
5	0.0	0.0	0.0	9.982859430358075E-5	0.0
6	0.0	0.0	0.0	9.982859430358075E-5	0.0
7	0.0	0.0	0.0	9.982859430358075E-5	0.0
8	0.0	0.0	0.0	9.982859430358075E-5	0.0
9	0.0	0.0	0.0	1.996571886071615E-4	0.0
10-11	0.0	0.0	0.0	1.996571886071615E-4	0.0
12-13	0.0	0.0	0.0	1.996571886071615E-4	0.0
14-15	0.0	0.0	0.0	1.996571886071615E-4	0.0
16-17	0.0	0.0	0.0	1.996571886071615E-4	0.0
18-19	0.0	0.0	0.0	1.996571886071615E-4	0.0
20-21	0.0	0.0	0.0	2.4957148575895186E-4	0.0
22-23	0.0	4.9914297151790374E-5	0.0	3.99314377214323E-4	0.0
24-25	0.0	9.982859430358075E-5	0.0	3.99314377214323E-4	0.0
26-27	0.0	9.982859430358075E-5	0.0	8.984573487322268E-4	0.0
28-29	0.0	9.982859430358075E-5	0.0	0.0021962290746787762	0.0
30-31	0.0	9.982859430358075E-5	0.0	0.004442372446509343	0.0
32-33	0.0	9.982859430358075E-5	0.0	0.010531916699027769	0.0
34-35	0.0	9.982859430358075E-5	0.0	0.018767775729073184	0.0
36-37	0.0	9.982859430358075E-5	0.0	0.03179540728569047	0.0
38-39	0.0	9.982859430358075E-5	0.0	0.05525512694703195	0.0
40-41	0.0	9.982859430358075E-5	0.0	0.08435516218652572	0.0
42-43	0.0	9.982859430358075E-5	0.0	0.10976153943678704	0.0
44-45	0.0	9.982859430358075E-5	0.0	0.13706465997881637	0.0
46-47	0.0	9.982859430358075E-5	0.0	0.17105629633918562	0.0
48-49	0.0	9.982859430358075E-5	0.0	0.2110875626549215	0.0
50-51	0.0	9.982859430358075E-5	0.0	0.2535646295310951	0.0
52-53	0.0	9.982859430358075E-5	0.0	0.3012826976082067	0.0
54-55	0.0	9.982859430358075E-5	0.0	0.3450076219131751	0.0
56-57	0.0	9.982859430358075E-5	0.0	0.3990148914314123	0.0
58-59	0.0	9.982859430358075E-5	0.0	0.4556676186986943	0.0
60-61	0.0	9.982859430358075E-5	0.0	0.5267954921399955	0.0
62-63	0.0	9.982859430358075E-5	0.0	0.6048614528853957	0.0
64-65	0.0	1.996571886071615E-4	0.0	0.6759893263266971	0.0
66-67	0.0	2.9948578291074225E-4	0.0	0.7571000591983564	0.0
68-69	0.0	2.9948578291074225E-4	0.0	0.8531351669184011	0.0
70-71	0.0	2.9948578291074225E-4	0.0	0.9542615329479284	0.0
72-73	0.0	2.9948578291074225E-4	0.0	1.06387332949326	0.0
74-75	0.0	2.9948578291074225E-4	0.0	1.183418071171798	0.0
76-77	0.0	2.9948578291074225E-4	0.0	1.3094516714800688	0.0
78-79	0.0	2.9948578291074225E-4	0.0	1.4592943915297434	0.0
80-81	0.0	2.9948578291074225E-4	0.0	1.6148273414547223	0.0
82-83	0.0	2.9948578291074225E-4	0.0	1.7939198396353462	0.0
84-85	0.0	2.9948578291074225E-4	0.0	1.980848882468801	0.0
86-87	0.0	2.9948578291074225E-4	0.0	2.172120469154462	0.0
88-89	0.0	2.9948578291074225E-4	0.0	2.386152975341339	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	255	0.0	54.030514	9
AAACCGT	275	0.0	48.34201	6
GGTATCA	990	0.0	45.63788	1
AACCGTG	335	0.0	41.101017	7
GTATCAA	1640	0.0	40.01956	1
ACCGTGC	390	0.0	38.958874	8
GTCTAAT	630	0.0	33.21614	1
TGCGCGG	235	0.0	30.340425	12-13
ATCAACG	2175	0.0	30.124456	3
CTAATGT	745	0.0	29.315744	3
GTGCGCG	240	0.0	28.712317	10-11
TCAACGC	2295	0.0	28.54932	4
TCTAATG	770	0.0	27.747328	2
GTACATG	3625	0.0	27.551695	1
GCGCGGC	245	0.0	27.161905	12-13
TAACGCA	105	6.92602E-5	27.13072	4
GTTATTA	760	0.0	26.864325	8
TACATGG	3735	0.0	26.31353	2
AATGCGG	240	9.094947E-12	25.717663	7
CGCAGAG	2605	0.0	25.15315	8
>>END_MODULE