FastQCFastQC Report
Fri 10 Feb 2017
SRR3224561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001717
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG20130.20095496033310806No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC17890.17859335520910596No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA17270.17240398236228396No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT16780.1675123812414085No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA14420.14395283298576345No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA13840.13816277451615575No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG13660.1363658598186913No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG13500.134768602309834No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12550.12528488585099384No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG12360.12338814255922581No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT12120.12099225629593988No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG11900.1187960272212611No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT11590.11570134079785009No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11440.11420391188329637No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT11360.11340528312886775No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG11360.11340528312886775No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG11160.11140871124279612No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC10380.10362208088711682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT250.004939218557.2372171
AACCGTG3050.051.3806467
GGTATCA10450.047.4694671
GTATCAA18900.046.9405751
AAACCGT3450.044.0476
GTGTACG554.8426846E-543.361531
ACCGTGC3800.042.4894148
CCGTGCG3850.040.7041449
ATCAACG24300.035.9581223
TCAACGC25100.034.8120464
AATGCCG1456.657501E-1032.7504659
CCGTACA600.003955531431.658789
GTCTAAT7200.029.8110521
ACGCAGA30100.029.344857
CAACGCA29850.029.272445
TATCAAC30400.029.2114722
CGCAGAG30300.029.1511548
AACGCAG30800.028.3695556
TGCGCGG2800.027.984112-13
GTACATG40600.027.1383361
Produced by FastQC (version 0.11.5)