FastQCFastQC Report
Fri 10 Feb 2017
SRR3224560_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224560_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225414
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG41820.34127241895392085No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24310.1983819345951654No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA21690.17700140523937216No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA20200.16484224923168822No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA17650.1440329553930345No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT14540.11865377741726468No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG14300.11669525564421493No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC13990.11416549835402566No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA13730.11204376643322174No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG13440.10967721929078664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT459.5678377E-442.2736431
GTATCAA16250.041.8509031
TCGCACC13950.038.523561
GGTATCA10200.038.2327771
CGCACCA15200.034.6758962
CTGTGCG16600.032.6268739
ATCAACG21650.032.0216143
TCAACGC22850.030.547764
AACCGTG2051.8189894E-1230.1118727
CCGTGCG2700.029.9132699
CGACCGT804.5026277E-429.6775686
ACCGTGC2900.029.47538
GTACATG43250.027.9299281
ACTGTGC21900.027.1050138
CGCAGAG26550.026.4716458
ACGCAGA26900.026.1250847
CACCACT21100.026.1050074
TACATGG46100.025.6475982
GCACCAC21450.025.2363073
ACCACTG22350.025.0699085
Produced by FastQC (version 0.11.5)