##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224560_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225414
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22109099455368	34.0	31.0	34.0	31.0	34.0
2	32.38956630167437	34.0	31.0	34.0	31.0	34.0
3	32.544010432392646	34.0	31.0	34.0	31.0	34.0
4	35.84681585162239	37.0	37.0	37.0	35.0	37.0
5	35.850509297266065	37.0	37.0	37.0	35.0	37.0
6	35.95103532357228	37.0	37.0	37.0	35.0	37.0
7	35.922850563156615	37.0	37.0	37.0	35.0	37.0
8	35.95753353560511	37.0	37.0	37.0	35.0	37.0
9	37.68972282020607	39.0	38.0	39.0	35.0	39.0
10-11	37.75943721876851	39.0	38.5	39.0	35.0	39.0
12-13	37.762489248531516	39.0	39.0	39.0	35.0	39.0
14-15	39.25108983576163	41.0	40.0	41.0	36.5	41.0
16-17	39.08196046397381	41.0	39.5	41.0	36.0	41.0
18-19	39.15664379548463	41.0	39.5	41.0	36.0	41.0
20-21	39.21527132871013	41.0	40.0	41.0	36.0	41.0
22-23	39.173074161059034	41.0	40.0	41.0	36.0	41.0
24-25	39.11631497599995	41.0	39.5	41.0	36.0	41.0
26-27	38.939165865576854	41.0	39.0	41.0	35.0	41.0
28-29	38.85395343940905	41.0	39.0	41.0	35.0	41.0
30-31	38.785614494366804	41.0	39.0	41.0	35.0	41.0
32-33	38.601432658676984	40.0	39.0	41.0	35.0	41.0
34-35	38.59998743281862	40.0	39.0	41.0	35.0	41.0
36-37	38.6039807771088	40.0	38.5	41.0	35.0	41.0
38-39	38.49302235815814	40.0	38.0	41.0	35.0	41.0
40-41	38.40115911846935	40.0	38.0	41.0	34.0	41.0
42-43	38.25150806176525	40.0	38.0	41.0	34.0	41.0
44-45	38.06468181365645	40.0	38.0	41.0	33.5	41.0
46-47	37.88868904713019	40.0	37.0	41.0	33.0	41.0
48-49	37.67175419898908	40.0	37.0	41.0	33.0	41.0
50-51	36.38310236377257	38.5	35.0	40.0	31.0	40.5
52-53	36.73472883450002	39.0	35.5	40.0	31.5	40.5
54-55	37.190887732635666	39.0	35.5	41.0	33.0	41.0
56-57	37.023247245420734	39.0	35.0	41.0	32.0	41.0
58-59	36.8534862503611	39.0	35.0	41.0	32.0	41.0
60-61	36.589159255565875	38.5	35.0	41.0	32.0	41.0
62-63	36.27993600530107	37.5	35.0	40.5	31.0	41.0
64-65	36.017117480296456	37.0	35.0	40.0	31.0	41.0
66-67	35.75452867357481	36.5	35.0	40.0	31.0	41.0
68-69	35.43689071611716	36.0	35.0	39.5	30.0	41.0
70-71	35.13646286071483	35.5	35.0	39.0	30.0	41.0
72-73	34.81621231681701	35.0	34.0	39.0	30.0	40.5
74-75	34.54449108627778	35.0	34.0	37.5	29.5	40.0
76-77	34.21395381479239	35.0	34.0	37.0	29.0	39.0
78-79	33.989508443676996	35.0	34.0	36.5	29.0	39.0
80-81	33.69216036376278	35.0	34.0	36.0	29.0	38.5
82-83	33.445708960400324	35.0	34.0	36.0	29.0	37.0
84-85	33.23064898883153	35.0	34.0	35.0	29.0	37.0
86-87	33.02269233091836	35.0	34.0	35.0	28.5	36.5
88-89	32.87592274937286	35.0	33.0	35.0	27.5	36.0
90-91	32.713249154979465	35.0	33.0	35.0	27.0	36.0
92-93	32.588876493984884	35.0	33.0	35.0	27.0	36.0
94-95	32.45659058897646	35.0	33.0	35.0	27.0	35.0
96-97	32.32501342403465	35.0	33.0	35.0	26.0	35.0
98-99	32.26850068629867	35.0	33.0	35.0	26.0	35.0
100-101	30.990313069705422	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	152.0
8	454.0
9	360.0
10	522.0
11	1112.0
12	1836.0
13	2196.0
14	2143.0
15	2207.0
16	2392.0
17	2539.0
18	2788.0
19	3104.0
20	3602.0
21	4535.0
22	5284.0
23	5504.0
24	5596.0
25	6160.0
26	6547.0
27	7150.0
28	8162.0
29	9844.0
30	12373.0
31	15594.0
32	21001.0
33	30029.0
34	49925.0
35	89805.0
36	192802.0
37	321486.0
38	328037.0
39	80151.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.807856832932806	17.79840023010277	12.694465848614275	24.69927708835015
2	16.532630223443036	18.972950344088073	38.36135245831405	26.133066974154833
3	20.849249761916614	24.20200243020505	27.380609444504746	27.568138363373595
4	13.106509310322878	15.598075425937683	37.17413053874037	34.121284724999065
5	14.448831170526857	37.907841757969145	33.530300779981296	14.1130262915227
6	32.29635045788607	35.63840465344773	18.063691128059578	14.001553760606619
7	29.126564573278905	29.88394126393203	21.04219471949888	19.947299443290188
8	26.674332103272853	34.5116017933531	19.667883670335087	19.14618243303896
9	26.902744705054783	15.237544209548773	18.982237839619916	38.87747324577653
10-11	26.795311625295614	24.297257906307582	27.06183379657814	21.84559667181867
12-13	26.970395311298873	22.010806143882803	27.211048674162363	23.807749870655957
14-15	24.006787138234266	24.03686965494496	24.184465829199876	27.771877377620896
16-17	24.290892936327563	26.794908752969498	25.259588757510006	23.65460955319293
18-19	24.134332482116903	26.404047964692133	25.9178057145096	23.54381383868136
20-21	24.972253595807445	24.906739835264528	25.81972817299878	24.30127839592925
22-23	24.989445645277325	25.36793256318527	25.222784667482706	24.419837124054702
24-25	24.424053215801504	25.251469147894223	25.708251714005875	24.6162259222984
26-27	24.44618716613324	26.21261059527637	25.745503152404005	23.595699086186382
28-29	24.090593056713892	26.27197828652194	25.768719795922028	23.86870886084213
30-31	24.255720923704153	25.34653594621899	25.94841416859935	24.44932896147751
32-33	24.152531307786592	25.649453980450687	26.050583721093446	24.147430990669275
34-35	24.74261759699171	25.063325537328605	25.667488701777526	24.52656816390216
36-37	24.041227688910926	25.31270724545412	26.262674494295585	24.38339057133937
38-39	24.186852538270323	25.738190020581676	25.441094141976006	24.633863299172
40-41	24.681701368514773	25.359225947346985	25.946571694619387	24.012500989518852
42-43	24.512522202595395	25.626681388828704	25.91888039753102	23.941916011044885
44-45	24.64494545893781	25.165239157589415	25.74915151319771	24.44066387027507
46-47	24.400814745057588	25.102618380400422	25.730610226421437	24.765956648120554
48-49	23.847491541634092	25.531330636013628	26.10583035610822	24.515347466244062
50-51	24.131803619021817	25.71718619176866	25.674506738130948	24.476503451078575
52-53	24.3230043071158	25.628930304370606	25.211683561637127	24.83638182687647
54-55	24.497353547454168	24.960462341706556	25.605305635483194	24.93687847535608
56-57	24.471045895936353	25.461446279153932	25.52012035121206	24.547387473697654
58-59	24.44277035911307	25.700837323795945	25.856728585152133	23.999663731938856
60-61	24.444468930212704	25.825711117722268	25.45960807263132	24.27021187943371
62-63	24.167069364086355	25.358958493868116	25.914721746966705	24.55925039507882
64-65	24.74798399442205	24.770232107006482	25.51180864500422	24.96997525356725
66-67	24.028302006140155	25.51604271418818	25.140353089481955	25.31530219018971
68-69	24.160610210100423	25.42463200191935	26.15899606173914	24.25576172624109
70-71	24.58817999467935	25.12999688268618	25.87505120718386	24.406771915450616
72-73	24.04832162844557	25.472738192969885	26.12986305036502	24.349077128219523
74-75	24.203575281496704	25.26676698650415	26.047523530823053	24.48213420117609
76-77	24.277835898724838	24.823937053110214	25.744401483906664	25.15382556425828
78-79	24.361902181187787	24.44258262297544	26.04166326587246	25.153851929964315
80-81	24.211806098482544	25.438867589140163	25.462873183913477	24.886453128463817
82-83	23.889033807728214	25.645082740492814	25.79359393414643	24.67228951763254
84-85	24.285112562476886	24.868393449099756	26.20066233869065	24.645831649732706
86-87	23.972367290815445	26.030245576904864	25.612717480411657	24.384669651868037
88-89	24.351183673469386	25.169714285714285	25.234816326530613	25.244285714285713
90-91	23.980181391758215	25.070996414273054	25.587352519230233	25.361469674738498
92-93	23.946784922394677	25.27642738869253	26.07626939520976	24.70051829370303
94-95	24.12258224567371	24.93720489565159	26.0055377203133	24.934675138361403
96-97	24.547581470425506	25.241265400917566	25.922830977938883	24.28832215071804
98-99	23.55965142995406	25.342488390587125	25.994353710381002	25.103506469077814
100-101	24.198604316047508	24.97951785834563	25.500358207858532	25.321519617748333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	2.0
4	1.5
5	2.5
6	4.5
7	9.5
8	14.0
9	19.0
10	27.5
11	29.0
12	28.5
13	37.0
14	42.5
15	43.0
16	45.5
17	51.5
18	62.5
19	70.0
20	92.0
21	129.0
22	165.0
23	195.0
24	222.0
25	292.5
26	417.5
27	565.0
28	713.5
29	931.5
30	1313.5
31	2120.5
32	3933.5
33	7182.5
34	12070.5
35	18698.5
36	26829.5
37	34069.5
38	38370.0
39	39590.5
40	38175.5
41	36081.5
42	34657.0
43	33925.0
44	33894.5
45	35601.0
46	39598.5
47	41670.0
48	43401.0
49	47674.5
50	52759.5
51	54865.5
52	55026.0
53	57123.0
54	60061.0
55	59573.0
56	51450.0
57	45064.5
58	43048.5
59	40032.0
60	33462.5
61	25833.5
62	21381.0
63	15850.0
64	10122.0
65	6821.0
66	5017.5
67	4253.5
68	3323.5
69	2115.5
70	1333.0
71	975.5
72	735.0
73	551.0
74	435.5
75	320.0
76	233.5
77	152.5
78	99.0
79	73.5
80	50.0
81	40.0
82	30.0
83	23.5
84	13.0
85	8.0
86	6.5
87	7.5
88	7.5
89	6.5
90	7.5
91	8.0
92	7.0
93	6.0
94	6.5
95	5.0
96	4.0
97	4.0
98	3.5
99	3.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13162898416371935
2	8.160507387707338E-5
3	2.4481522163122016E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.036722283244683025
16-17	0.07189407008570164
18-19	0.024848744995568845
20-21	0.03921123799793376
22-23	0.06536566417553578
24-25	0.017463485809693705
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.021747752188240058
38-39	0.004243463841607816
40-41	0.00579396024527221
42-43	0.004121056230792205
44-45	0.011791933175237104
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.080253693853669E-5
58-59	0.016402619849291752
60-61	0.017341078198878094
62-63	0.027908935265959098
64-65	0.04822859866135037
66-67	0.01636181731235321
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01713706551418541
80-81	0.05708274917701283
82-83	0.04818779612441183
84-85	0.02945943166962349
86-87	0.0576131821572138
88-89	0.03378450058510838
90-91	0.0
92-93	0.0038354384722224487
94-95	0.0
96-97	0.0
98-99	6.120380540780504E-4
100-101	0.046229274351362065
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1225414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.486119766166944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.208565656954	35.011131625862006
2	15.147208868736719	14.688587666654293
3	5.456695185819672	7.9372192892135764
4	2.5704401123776703	4.985226685620133
5	1.3400382750742958	3.2486628148250034
6	0.8065586449673445	2.346413943499439
7	0.5315531789851948	1.8041065768854048
8	0.3535135025348666	1.3712398418290161
9	0.24003905584004287	1.0474706169016157
>10	1.1491864617480458	9.725794438927135
>50	0.09599956295939617	3.285234955151411
>100	0.08744239455193735	9.021576290539581
>500	0.00952679425661816	3.079503572246715
>1k	0.0032323051942097325	2.447831681844793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	4182	0.34127241895392085	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2431	0.1983819345951654	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	2169	0.17700140523937216	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	2020	0.16484224923168822	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1765	0.1440329553930345	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1454	0.11865377741726468	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1430	0.11669525564421493	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1399	0.11416549835402566	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1373	0.11204376643322174	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1344	0.10967721929078664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6321014775414676E-4	0.0
2	0.0	0.0	0.0	1.6321014775414676E-4	0.0
3	0.0	0.0	0.0	1.6321014775414676E-4	0.0
4	0.0	0.0	0.0	1.6321014775414676E-4	0.0
5	0.0	0.0	0.0	1.6321014775414676E-4	0.0
6	0.0	0.0	0.0	1.6321014775414676E-4	0.0
7	0.0	0.0	0.0	1.6321014775414676E-4	0.0
8	0.0	0.0	0.0	1.6321014775414676E-4	0.0
9	0.0	0.0	0.0	1.6321014775414676E-4	0.0
10-11	0.0	0.0	0.0	1.6321014775414676E-4	0.0
12-13	0.0	0.0	0.0	2.4481522163122016E-4	0.0
14-15	0.0	0.0	0.0	3.264202955082935E-4	4.080253693853669E-5
16-17	0.0	0.0	0.0	3.264202955082935E-4	8.160507387707338E-5
18-19	0.0	0.0	0.0	4.488279063239036E-4	8.160507387707338E-5
20-21	0.0	0.0	0.0	5.712355171395136E-4	8.160507387707338E-5
22-23	0.0	0.0	0.0	7.344456648936604E-4	8.160507387707338E-5
24-25	0.0	0.0	0.0	7.752482018321971E-4	8.160507387707338E-5
26-27	0.0	0.0	0.0	8.976558126478071E-4	8.160507387707338E-5
28-29	0.0	0.0	0.0	0.0017953116252956143	8.160507387707338E-5
30-31	0.0	0.0	0.0	0.004325068915484889	8.160507387707338E-5
32-33	0.0	0.0	0.0	0.009711003791371731	1.6321014775414676E-4
34-35	0.0	0.0	0.0	0.016851447755615652	1.6321014775414676E-4
36-37	0.0	0.0	0.0	0.03105073061022642	2.0401268469268346E-4
38-39	0.0	0.0	0.0	0.0587148506545543	2.8561775856975687E-4
40-41	0.0	0.0	0.0	0.09098965737293682	3.264202955082935E-4
42-43	0.0	0.0	0.0	0.12297884633274958	3.672228324468302E-4
44-45	0.0	0.0	0.0	0.15847705346927649	4.080253693853669E-4
46-47	0.0	0.0	0.0	0.20250299082595757	5.712355171395136E-4
48-49	0.0	0.0	0.0	0.2519148630585255	6.52840591016587E-4
50-51	0.0	0.0	0.0	0.3056926067435169	6.52840591016587E-4
52-53	0.0	0.0	0.0	0.3619185026448204	7.344456648936604E-4
54-55	0.0	0.0	0.0	0.4237343461067035	8.568532757092705E-4
56-57	0.0	0.0	0.0	0.5022792297133867	8.976558126478071E-4
58-59	0.0	0.0	0.0	0.5851491822355547	8.976558126478071E-4
60-61	0.0	0.0	0.0	0.6794846476374514	8.976558126478071E-4
62-63	0.0	0.0	0.0	0.7797364808954361	8.976558126478071E-4
64-65	0.0	0.0	0.0	0.8806411547444374	8.976558126478071E-4
66-67	0.0	0.0	0.0	0.9997437600680259	8.976558126478071E-4
68-69	0.0	0.0	0.0	1.134759354797644	8.976558126478071E-4
70-71	0.0	0.0	0.0	1.2874016454847097	8.976558126478071E-4
72-73	0.0	0.0	0.0	1.4544880342480173	0.0012240761081561008
74-75	0.0	0.0	0.0	1.6244714031339613	0.0012240761081561008
76-77	0.0	0.0	0.0	1.8048186164022932	0.001305681182033174
78-79	0.0	0.0	0.0	2.012870752251892	0.0013872862559102476
80-81	0.0	0.0	0.0	2.252340841544164	0.0014280887928487841
82-83	0.0	0.0	0.0	2.5074383024838953	0.0015504964036643942
84-85	0.0	0.0	0.0	2.7645758902705535	0.0015504964036643942
86-87	0.0	0.0	0.0	3.031546889459399	0.0015504964036643942
88-89	0.0	0.0	0.0	3.333077637435185	0.0016321014775414677
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAT	45	9.5678377E-4	42.273643	1
GTATCAA	1625	0.0	41.850903	1
TCGCACC	1395	0.0	38.52356	1
GGTATCA	1020	0.0	38.232777	1
CGCACCA	1520	0.0	34.675896	2
CTGTGCG	1660	0.0	32.626873	9
ATCAACG	2165	0.0	32.021614	3
TCAACGC	2285	0.0	30.54776	4
AACCGTG	205	1.8189894E-12	30.111872	7
CCGTGCG	270	0.0	29.913269	9
CGACCGT	80	4.5026277E-4	29.677568	6
ACCGTGC	290	0.0	29.4753	8
GTACATG	4325	0.0	27.929928	1
ACTGTGC	2190	0.0	27.105013	8
CGCAGAG	2655	0.0	26.471645	8
ACGCAGA	2690	0.0	26.125084	7
CACCACT	2110	0.0	26.105007	4
TACATGG	4610	0.0	25.647598	2
GCACCAC	2145	0.0	25.236307	3
ACCACTG	2235	0.0	25.069908	5
>>END_MODULE