FastQCFastQC Report
Fri 10 Feb 2017
SRR3224560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225414
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG38520.3143427445744867No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA22310.18206091981975073No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21990.17944955745568436No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA21420.17479806824469118No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT17540.14313529958038673No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG14900.12159156007683933No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG14110.11514475924055054No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA13820.11277821209811541No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC13510.11024845480792614No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG13040.10641301633570369No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT13020.10624980618794955No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC12940.10559696559693296No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT12870.10502573007979345No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT12650.10323041845449782No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG12600.10282239308511247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10150.046.536291
GTATCAA18050.044.4073261
ATCAACG22500.035.458843
CCGTGCG2250.033.770329
TCAACGC24000.033.242664
AAACCGT2000.033.2426576
TCGCACC14050.031.241661
CAACGCA26900.029.658885
ACCGTGC2450.029.0752128
CGCACCA15250.028.960822
AACGCAG27900.028.5958376
GACCGCA11550.028.370367
GTACATG48700.028.2153931
ACGCAGA28450.028.2099387
CGCAGAG28450.028.0430158
ACCGCAA11350.028.0334578
CTGTGCG17300.026.9015739
CCGCAAG12050.026.7990639
AGACCGC12850.026.608916
TACATGG52350.026.1260362
Produced by FastQC (version 0.11.5)