##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225414
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04632556833854	34.0	33.0	34.0	31.0	34.0
2	33.17744778499348	34.0	33.0	34.0	31.0	34.0
3	33.29324701692652	34.0	34.0	34.0	31.0	34.0
4	36.55569383081963	37.0	37.0	37.0	35.0	37.0
5	36.57424021595967	37.0	37.0	37.0	35.0	37.0
6	36.608323391115164	37.0	37.0	37.0	35.0	37.0
7	36.58139045253278	37.0	37.0	37.0	35.0	37.0
8	36.56143719591909	37.0	37.0	37.0	35.0	37.0
9	38.360135431780606	39.0	39.0	39.0	37.0	39.0
10-11	38.37231417300602	39.0	39.0	39.0	37.0	39.0
12-13	38.36358324615192	39.0	39.0	39.0	37.0	39.0
14-15	39.902722671684835	41.0	40.0	41.0	38.0	41.0
16-17	39.90423603777988	41.0	40.0	41.0	38.0	41.0
18-19	39.87899395632823	41.0	40.0	41.0	38.0	41.0
20-21	39.874806799987596	41.0	40.0	41.0	38.0	41.0
22-23	39.77765677558768	41.0	40.0	41.0	38.0	41.0
24-25	39.735697894752306	41.0	40.0	41.0	37.0	41.0
26-27	39.66203136246199	41.0	40.0	41.0	37.0	41.0
28-29	39.62193511743786	41.0	40.0	41.0	37.0	41.0
30-31	39.565000073444565	41.0	40.0	41.0	37.0	41.0
32-33	39.52339413455371	41.0	39.5	41.0	37.0	41.0
34-35	39.440194089507706	41.0	39.0	41.0	36.0	41.0
36-37	39.32548265320945	41.0	39.0	41.0	36.0	41.0
38-39	39.209096272769855	40.5	39.0	41.0	35.5	41.0
40-41	39.05798448524335	40.0	39.0	41.0	35.0	41.0
42-43	38.949695776284585	40.0	38.5	41.0	35.0	41.0
44-45	38.67923003980695	40.0	38.0	41.0	35.0	41.0
46-47	38.43970731524203	40.0	38.0	41.0	35.0	41.0
48-49	38.350116368835344	40.0	37.5	41.0	35.0	41.0
50-51	38.18897654180546	40.0	37.0	41.0	34.0	41.0
52-53	37.926058050585354	40.0	36.5	41.0	34.0	41.0
54-55	37.72366685871061	39.0	36.0	41.0	34.0	41.0
56-57	37.486775897778216	39.0	35.0	41.0	33.0	41.0
58-59	37.20178894642953	39.0	35.0	41.0	33.0	41.0
60-61	36.92871062351173	38.0	35.0	40.5	33.0	41.0
62-63	36.68481631513921	37.5	35.0	40.0	33.0	41.0
64-65	36.39293944740308	37.0	35.0	40.0	32.0	41.0
66-67	36.07669163237893	36.5	35.0	40.0	32.0	41.0
68-69	35.79581716872828	36.0	35.0	39.0	31.5	41.0
70-71	35.490585222626805	35.5	35.0	39.0	31.0	41.0
72-73	35.17854618928787	35.0	34.0	38.5	31.0	40.0
74-75	34.86391741892944	35.0	34.0	37.0	31.0	40.0
76-77	33.0097946489921	34.5	32.0	35.5	28.0	38.0
78-79	34.46097155736756	35.0	34.0	36.0	31.0	39.0
80-81	34.43646310553005	35.0	34.0	36.0	31.0	38.5
82-83	34.24420481567862	35.0	34.0	36.0	31.0	37.0
84-85	34.04633984922647	35.0	34.0	35.5	31.0	37.0
86-87	33.85840622026515	35.0	34.0	35.0	31.0	36.5
88-89	33.69827584799913	35.0	34.0	35.0	31.0	36.0
90-91	33.545740459958836	35.0	34.0	35.0	30.5	36.0
92-93	33.45582921363719	35.0	34.0	35.0	30.5	36.0
94-95	33.3470435297785	35.0	34.0	35.0	30.0	35.0
96-97	33.28714622160348	35.0	34.0	35.0	30.0	35.0
98-99	33.24185662967781	35.0	34.0	35.0	30.0	35.0
100-101	32.28128575322299	34.5	32.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	21.0
15	61.0
16	145.0
17	251.0
18	486.0
19	762.0
20	1142.0
21	1721.0
22	2353.0
23	2602.0
24	2925.0
25	3762.0
26	4990.0
27	6704.0
28	8371.0
29	9731.0
30	11285.0
31	13413.0
32	17172.0
33	24546.0
34	42361.0
35	82939.0
36	204470.0
37	348209.0
38	358684.0
39	76290.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.254020720963645	17.578724007615662	12.647803448357822	24.51945182306287
2	16.480307879622725	19.045563376948525	38.409141726796	26.064987016632745
3	20.726138268372974	24.372416179348367	27.365200658716155	27.5362448935625
4	13.205251449714137	15.721788717935326	37.293192341527025	33.77976749082351
5	14.460011065648018	37.325589555856226	34.02123690442577	14.193162474069986
6	32.56972745537426	35.18386439195243	18.239468457190792	14.006939695482506
7	29.196092096222177	29.74366214193734	21.03248371570751	20.02776204613298
8	26.779357833352645	34.352308689145055	19.488434112879403	19.379899364622894
9	26.905600882640478	15.217958991818275	19.01251332202831	38.86392680351293
10-11	26.88295547463959	24.176727212191146	27.11402024132252	21.82629707184674
12-13	27.339372652834065	22.043937803876894	27.07097356485237	23.545715978436675
14-15	24.0903890440292	24.200719103911005	24.325860484701497	27.383031367358296
16-17	24.144493208009703	26.818120243444255	25.350657002449783	23.68672954609626
18-19	24.019719050051656	26.334936601018104	26.246843923767806	23.398500425162435
20-21	24.810297334472008	25.076454346299528	26.12961679184302	23.983631527385445
22-23	24.971968291118475	25.318263943633006	25.553167215875984	24.15660054937253
24-25	24.67468031107956	25.104128414163423	25.76129621922459	24.459895055532435
26-27	24.44056540412881	26.323110457169115	25.640102857133527	23.596221281568546
28-29	24.196633683566255	26.13016423543813	26.068672855248394	23.60452922574722
30-31	24.21382146562913	25.258128839800374	26.125351975325717	24.402697719244774
32-33	24.23401397405285	25.56654322539158	26.402056774281995	23.79738602627357
34-35	24.84816706926944	25.201818241169942	25.544982698961938	24.40503199059868
36-37	24.011563618261867	25.36932988954582	26.171724219537374	24.44738227265494
38-39	24.271878728331814	25.626318942006538	25.584985972087797	24.51681635757385
40-41	24.404772591140627	25.55409845162533	26.04075030969126	24.00037864754279
42-43	24.436598569952686	25.581966584354348	26.103423006428848	23.87801183926412
44-45	24.61380323174173	25.197979783965668	25.91816223508332	24.270054749209287
46-47	24.264681563109804	25.145761136706163	25.881641017851475	24.707916282332555
48-49	23.9062382691749	25.24028810090386	26.324869185999066	24.528604443922166
50-51	24.27253156892283	25.42059255076244	25.89973674203167	24.407139138283064
52-53	24.39073651843377	25.571806752656652	25.400599307662553	24.636857421247026
54-55	24.524446431981357	24.88738499804964	25.842653992854657	24.745514577114346
56-57	24.608921678241778	25.30550953620657	25.4912554169081	24.59431336864355
58-59	24.647947977244332	25.523552248687775	25.827856469638355	24.000643304429534
60-61	24.472417124439154	25.742860769547555	25.50356333994398	24.281158766069314
62-63	24.247029983336244	25.252853321408114	25.910835032078953	24.589281663176692
64-65	24.505799672600443	24.95458677638741	25.703680552042	24.835932998970144
66-67	24.051585831400654	25.322748067183827	25.469759607773373	25.15590649364215
68-69	24.48776495127361	25.179204742234052	26.059193056387475	24.27383725010486
70-71	24.799088308114644	25.124366132588662	25.856975683320087	24.21956987597661
72-73	24.09965121991425	25.366447584245	26.045360996365307	24.48854019947544
74-75	24.273674039957108	25.13913665096041	26.06086596040195	24.526323348680528
76-77	24.25789214480197	24.74405536425927	25.938144043857918	25.059908447080836
78-79	24.377139466814977	24.56753645637171	25.798547253831504	25.25677682298181
80-81	23.95809020472585	25.42326820342989	25.637819848434646	24.980821743409617
82-83	23.830253601973876	25.392186824840284	26.07722592825471	24.70033364493112
84-85	24.278183790823967	24.885496432385057	26.201884529808055	24.634435246982918
86-87	23.694318817966824	25.995418691152537	25.894718029988233	24.4155444608924
88-89	24.365275735384124	24.961645615277774	25.260932223721944	25.41214642561616
90-91	24.18101147856969	25.0959267643425	25.43899449492172	25.284067262166094
92-93	24.063305977941322	25.314230665811987	25.98988015065935	24.632583205587338
94-95	24.199250212581216	24.960013513800234	25.687114722044956	25.15362155157359
96-97	24.480706112383245	25.259871357761536	25.800504972197153	24.458917557658065
98-99	23.087911513986292	25.52308852355204	26.10481029268476	25.284189669776907
100-101	23.84708916246664	25.128368866140345	25.697699486197894	25.326842485195115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	1.5
3	3.0
4	2.0
5	1.0
6	1.5
7	8.5
8	15.0
9	21.5
10	33.0
11	35.5
12	38.0
13	46.0
14	44.5
15	43.5
16	51.5
17	68.0
18	73.0
19	78.0
20	106.5
21	131.0
22	156.0
23	197.0
24	259.0
25	341.0
26	463.5
27	606.5
28	750.5
29	948.0
30	1297.5
31	2081.5
32	3711.5
33	6947.0
34	11825.0
35	18067.0
36	26102.0
37	33741.0
38	38188.0
39	39315.5
40	38104.5
41	36195.0
42	35012.0
43	34733.0
44	35012.0
45	36343.5
46	38818.5
47	41212.5
48	44002.5
49	48826.5
50	54156.5
51	55286.5
52	53900.5
53	56409.0
54	60705.0
55	60118.0
56	51870.5
57	45262.0
58	43319.5
59	40848.0
60	34294.0
61	25979.0
62	21081.5
63	15192.5
64	9380.5
65	6189.0
66	4643.0
67	3973.0
68	2950.5
69	1797.0
70	1085.0
71	751.0
72	593.0
73	466.5
74	362.5
75	252.5
76	191.0
77	130.0
78	70.0
79	39.0
80	19.5
81	9.5
82	6.5
83	6.5
84	5.5
85	4.0
86	1.5
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4322620763268577
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.752482018321971E-4
22-23	0.001305681182033174
24-25	3.264202955082935E-4
26-27	0.0020401268469268344
28-29	0.0031825978812058623
30-31	6.936431279551237E-4
32-33	0.0
34-35	0.004406673989361963
36-37	0.0015504964036643942
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008119704850768801
46-47	0.07291413350916508
48-49	8.160507387707337E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.006854826205674165
58-59	0.039864078588950344
60-61	0.030153074797578613
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.0401268469268343E-4
78-79	0.006487603373227334
80-81	0.0055491450236409895
82-83	7.752482018321971E-4
84-85	0.0012648786450946373
86-87	0.0
88-89	0.0
90-91	0.0
92-93	4.080253693853669E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06675295043144602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1225414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.94207433218731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96303859778264	33.311522326949905
2	15.702094955011356	14.74177817098413
3	5.697936073029122	8.024188160405535
4	2.7065743398891655	5.08208855394672
5	1.4348847500547104	3.367823329759511
6	0.8576013336289154	2.415455133035491
7	0.5429336510517014	1.7840502263580373
8	0.38609143597696666	1.4499146309321365
9	0.26933727595878915	1.1378925385637695
>10	1.2313053867158008	10.117556136328258
>50	0.0995101710265788	3.2949791052298463
>100	0.09493134477922308	9.399548008425871
>500	0.010276967349587109	3.335499557013829
>1k	0.0034837177456227488	2.5377041220670504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3852	0.3143427445744867	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	2231	0.18206091981975073	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2199	0.17944955745568436	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	2142	0.17479806824469118	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1754	0.14313529958038673	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1490	0.12159156007683933	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1411	0.11514475924055054	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1382	0.11277821209811541	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1351	0.11024845480792614	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1304	0.10641301633570369	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1302	0.10624980618794955	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1294	0.10559696559693296	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1287	0.10502573007979345	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1265	0.10323041845449782	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1260	0.10282239308511247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.6321014775414676E-4	1.6321014775414676E-4
9	0.0	0.0	0.0	1.6321014775414676E-4	1.6321014775414676E-4
10-11	0.0	0.0	0.0	1.6321014775414676E-4	1.6321014775414676E-4
12-13	0.0	0.0	0.0	2.4481522163122016E-4	1.6321014775414676E-4
14-15	0.0	0.0	0.0	3.264202955082935E-4	2.448152216312201E-4
16-17	0.0	0.0	0.0	3.264202955082935E-4	3.264202955082935E-4
18-19	0.0	0.0	0.0	4.488279063239036E-4	3.264202955082935E-4
20-21	0.0	0.0	0.0	5.712355171395136E-4	3.672228324468302E-4
22-23	0.0	0.0	0.0	5.712355171395136E-4	4.896304432624403E-4
24-25	0.0	0.0	0.0	5.712355171395136E-4	4.896304432624403E-4
26-27	0.0	0.0	0.0	5.712355171395136E-4	4.896304432624403E-4
28-29	0.0	0.0	0.0	0.0015504964036643942	5.30432980200977E-4
30-31	0.0	0.0	0.0	0.004080253693853669	5.712355171395136E-4
32-33	0.0	0.0	0.0	0.009629398717494658	5.712355171395136E-4
34-35	0.0	0.0	0.0	0.01689225029255419	5.712355171395136E-4
36-37	0.0	0.0	0.0	0.03109153314716496	5.712355171395136E-4
38-39	0.0	0.0	0.0	0.058470035432923076	6.120380540780503E-4
40-41	0.0	0.0	0.0	0.09123447259456804	6.52840591016587E-4
42-43	0.0	0.0	0.0	0.12310125394356519	6.52840591016587E-4
44-45	0.0	0.0	0.0	0.15888507883866188	6.52840591016587E-4
46-47	0.0	0.0	0.0	0.20311502888003563	6.52840591016587E-4
48-49	0.0	0.0	0.0	0.25285332140811184	6.52840591016587E-4
50-51	0.0	0.0	0.0	0.30703909046248856	6.52840591016587E-4
52-53	0.0	0.0	0.0	0.36404023456562434	6.936431279551238E-4
54-55	0.0	0.0	0.0	0.4264273135446469	7.752482018321971E-4
56-57	0.0	0.0	0.0	0.5058290504270393	8.568532757092705E-4
58-59	0.0	0.0	0.0	0.5897598689096093	8.976558126478071E-4
60-61	0.0	0.0	0.0	0.6861762636953715	8.976558126478071E-4
62-63	0.0	0.0	0.0	0.7875297654506968	8.976558126478071E-4
64-65	0.0	0.0	0.0	0.8885160443735749	8.976558126478071E-4
66-67	0.0	0.0	0.0	1.0085979105836884	8.976558126478071E-4
68-69	0.0	0.0	0.0	1.1452456067908479	8.976558126478071E-4
70-71	0.0	0.0	0.0	1.2992343811968854	9.384583495863439E-4
72-73	0.0	0.0	0.0	1.4679120689007958	0.0015504964036643942
74-75	0.0	0.0	0.0	1.6401803798552979	0.0015504964036643942
76-77	0.0	0.0	0.0	1.8223229047489258	0.0015504964036643942
78-79	0.0	0.0	0.0	2.031272696411172	0.0015504964036643942
80-81	0.0	0.0	0.0	2.2726197024026167	0.0015504964036643942
82-83	0.0	0.0	0.0	2.529757290189275	0.001713706551418541
84-85	0.0	0.0	0.0	2.788118954084089	0.001713706551418541
86-87	0.0	0.0	0.0	3.0575789080261853	0.001713706551418541
88-89	0.0	0.0	0.0	3.3614354006074683	0.0017953116252956143
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1015	0.0	46.53629	1
GTATCAA	1805	0.0	44.407326	1
ATCAACG	2250	0.0	35.45884	3
CCGTGCG	225	0.0	33.77032	9
TCAACGC	2400	0.0	33.24266	4
AAACCGT	200	0.0	33.242657	6
TCGCACC	1405	0.0	31.24166	1
CAACGCA	2690	0.0	29.65888	5
ACCGTGC	245	0.0	29.075212	8
CGCACCA	1525	0.0	28.96082	2
AACGCAG	2790	0.0	28.595837	6
GACCGCA	1155	0.0	28.37036	7
GTACATG	4870	0.0	28.215393	1
ACGCAGA	2845	0.0	28.209938	7
CGCAGAG	2845	0.0	28.043015	8
ACCGCAA	1135	0.0	28.033457	8
CTGTGCG	1730	0.0	26.901573	9
CCGCAAG	1205	0.0	26.799063	9
AGACCGC	1285	0.0	26.60891	6
TACATGG	5235	0.0	26.126036	2
>>END_MODULE