FastQCFastQC Report
Fri 10 Feb 2017
SRR3224559_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224559_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696041
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG30650.4403476232003574No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA23010.3305839742199095No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA18740.26923701333685807No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC16890.24265811927745634No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC15000.21550454642758113No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA14750.21191280398712145No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG14510.20846473124428014No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA14480.208033722151425No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA14400.2068843645704779No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG14190.20386730092049177No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA14030.20156858575859754No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC13290.1909370281348369No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC13000.18677060690390365No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG12620.18131115839440493No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA12310.17685739776823492No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA12180.17498969169919587No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT11940.17154161895635459No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT11690.1679498765158949No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC11410.16392712498258005No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG10870.15616896131118713No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG10800.1551632734278584No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT10540.15142786128978034No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC10500.1508531824993068No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG9970.14323868852553226No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9500.13648621273746805No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC9200.1321761218089164No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA9000.12930272785654867No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA8560.12298126116133963No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA8550.12283759146372124No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC8430.1211135550923006No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC8340.11982052781373512No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG8320.11953318841849833No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA8020.1152230974899467No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT8020.1152230974899467No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA8010.11507942779232833No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA7990.11479208839709155No Hit
ACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCG7910.11364273081614444No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT7800.11206236414234218No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC7760.11148768535186863No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC7730.11105667625901348No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA7640.10976364898044799No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT7640.10976364898044799No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG7460.10717759442331702No Hit
ACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTG7400.1063155762376067No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG7380.10602823684236992No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC7290.10473520956380443No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA7250.10416053077333089No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT7180.10315484289000215No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG7180.10315484289000215No Hit
GTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGA7170.10301117319238377No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA7150.102723833797147No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1650.071.99729
AAACCGT1850.066.72476
ACCGTGC1900.062.4744768
AACCGTG2150.057.4142767
GGTATCA9300.056.2487561
GTATCAA21050.047.442811
CACGTAG459.645225E-442.201957
ATCGCTA500.001612347338.0145239
ATCAACG26650.036.1646163
CGGCTTA1650.036.00507416-17
AATGCGG3350.035.430747
TACACGT550.00258501134.5288665
TCAACGC28350.034.1634834
TGCGCGG1750.033.94642312-13
AAATGCG3650.032.5186236
ACGTAGA600.003957796431.6537368
ATACCGT902.419535E-531.6514636
CGCAGAG30950.031.1423668
ATGCGGG3700.030.7982278
TATCAAC32250.030.3265152
Produced by FastQC (version 0.11.5)