FastQCFastQC Report
Fri 10 Feb 2017
SRR3224559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696041
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT21430.3078841619962042No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG20740.29797095286053554No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG18830.2705300406154235No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG16720.24021573441794378No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA16680.23964105562747023No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA16640.23906637683699666No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT16530.2374860101631944No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG15760.22642344344657858No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG15610.22426839798230275No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA15490.22254436161088212No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG15230.21880894947280405No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15200.2183779403799489No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC14200.20401097061811013No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC13480.19366675238958625No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT12510.17973079172060266No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12070.1734093250253936No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG12000.17240363714206489No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC11920.1712542795611178No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC10840.15573795221833195No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA10810.1553069431254768No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC10760.15458859463738486No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG10680.15343923705643778No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT10600.15228987947549066No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT10560.15171520068501712No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC10350.14869813703503099No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA10240.14711777036122872No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC9760.14022162487554612No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT9300.1336128187851003No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA9250.13289447029700838No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA9120.13102676422796933No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC9030.12973373694940385No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG8980.12901538846131191No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT8950.12858437936845674No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA8920.12815337027560159No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG8720.12527997632323384No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA8580.12326860055657642No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC8530.12255025206848447No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT8480.12183190358039254No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC8410.12082621569706382No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA8390.12053887630182705No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA8290.11910217932564317No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA7990.11479208839709155No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG7540.10832695200426411No Hit
ACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCG7450.10703392472569864No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA7440.10689025502808025No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT7380.10602823684236992No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA7340.10545355805189638No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG7330.10530988835427799No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC7230.10387319137809412No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG7150.102723833797147No Hit
CTGCTGGGCGATCTGTTGCGCGAAGTGGTATGGCCGACAGATGTGTCCAC7150.102723833797147No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT7130.10243649440191023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.068.372461
CCGTGCG1800.055.40469
AAACCGT1900.049.9891136
ACCGTGC2000.049.8641368
GTATCAA18950.049.827411
AACCGTG2200.045.3310367
ATCAACG26650.035.1049233
TCAACGC28300.033.058174
GTATTAT902.3472312E-531.7922381
ACGTGGC1701.09139364E-1030.72869
TTATGCA1252.1559572E-730.393384
TGCGCGG1650.030.2206912-13
CAAGTCG404.4790737E-429.70450856-57
TAGACCC804.497573E-429.6810345
ACCCTTG1652.6502676E-928.7816097
CGCAGAG33250.028.5652078
TATACAG3200.028.1969815
TATCAAC33650.028.0845222
ACGCAGA34050.027.75467
GTCTTAT700.00822891227.250491
Produced by FastQC (version 0.11.5)