FastQCFastQC Report
Fri 10 Feb 2017
SRR3224558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829871
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12170.14664929850543035No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA10680.12869470074264555No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC10580.1274896941813848No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT9350.11266811347787789No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA9220.11110160494823894No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG8480.10218455639490957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6800.051.0320781
CCGTGCG2650.050.1972169
GTATCAA13650.046.317841
ACCGTGC3200.046.004048
AAACCGT2800.044.096046
AACCGTG3200.040.0680357
ATCAACG17850.035.3832443
TCAACGC18650.033.8654634
GCGTTAT600.00393540331.6911541
TATCAAC20650.030.1255842
CGCAGAG21650.029.3921348
TGCGCGG2150.028.72754712-13
ACGCAGA22250.028.599547
CAACGCA23750.026.5933045
AACGCAG24400.026.274126
GTCTAAT5750.024.8017731
GTACATG30950.024.5747221
CCCATAA7050.024.2492122
TACATGG30400.024.0564422
GCCCATA7150.023.9345781
Produced by FastQC (version 0.11.5)