##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224558_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	829871
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.106976867489045	34.0	31.0	34.0	30.0	34.0
2	32.27658154098649	34.0	31.0	34.0	31.0	34.0
3	32.4189265560551	34.0	31.0	34.0	31.0	34.0
4	35.71487255248105	37.0	35.0	37.0	35.0	37.0
5	35.728926544005034	37.0	37.0	37.0	35.0	37.0
6	35.825721106051425	37.0	37.0	37.0	35.0	37.0
7	35.787465762751076	37.0	37.0	37.0	35.0	37.0
8	35.824969181957194	37.0	37.0	37.0	35.0	37.0
9	37.534270989105536	39.0	38.0	39.0	35.0	39.0
10-11	37.60666115577	39.0	38.5	39.0	35.0	39.0
12-13	37.61582402566182	39.0	38.5	39.0	35.0	39.0
14-15	39.09420500294624	41.0	40.0	41.0	36.0	41.0
16-17	38.93240093942312	41.0	39.5	41.0	35.5	41.0
18-19	39.01919695952745	41.0	39.5	41.0	36.0	41.0
20-21	39.0871141418365	41.0	40.0	41.0	36.0	41.0
22-23	39.039768229038	41.0	40.0	41.0	36.0	41.0
24-25	38.981221780252596	41.0	39.0	41.0	35.5	41.0
26-27	38.81574244671762	41.0	39.0	41.0	35.0	41.0
28-29	38.72823728025199	41.0	39.0	41.0	35.0	41.0
30-31	38.635553598089345	41.0	39.0	41.0	35.0	41.0
32-33	38.443593642867384	40.5	39.0	41.0	34.0	41.0
34-35	38.44250130441961	40.0	38.0	41.0	34.0	41.0
36-37	38.45197627101079	40.0	38.0	41.0	34.0	41.0
38-39	38.350723787191015	40.0	38.0	41.0	34.0	41.0
40-41	38.26224979545013	40.0	38.0	41.0	34.0	41.0
42-43	38.12212018494441	40.0	38.0	41.0	33.0	41.0
44-45	37.96102828029899	40.0	38.0	41.0	33.0	41.0
46-47	37.78932448537182	40.0	38.0	41.0	33.0	41.0
48-49	37.59366877502648	40.0	37.0	41.0	32.0	41.0
50-51	36.34031674802469	38.5	35.5	40.0	30.5	40.5
52-53	36.70277187659287	39.0	36.0	40.0	31.0	40.5
54-55	37.20308999832504	40.0	36.0	41.0	32.0	41.0
56-57	37.076766750494954	40.0	35.5	41.0	31.0	41.0
58-59	36.951781662451154	39.5	35.0	41.0	31.0	41.0
60-61	36.71956665554043	39.0	35.0	41.0	31.0	41.0
62-63	36.43221958593564	39.0	35.0	41.0	31.0	41.0
64-65	36.17116395198772	38.0	35.0	41.0	30.0	41.0
66-67	35.90625229704376	37.5	35.0	40.0	30.0	41.0
68-69	35.5764667038612	37.0	35.0	40.0	29.5	41.0
70-71	35.26100683118219	36.5	35.0	39.5	29.0	41.0
72-73	34.914488516889975	36.0	34.5	39.0	29.0	41.0
74-75	34.61047620654294	35.5	34.0	39.0	29.0	40.5
76-77	34.23627889153856	35.0	34.0	37.5	28.5	39.5
78-79	33.96566454304344	35.0	34.0	37.0	28.5	39.0
80-81	33.63755933151056	35.0	34.0	36.5	28.0	39.0
82-83	33.355206411598914	35.0	34.0	36.0	27.0	37.5
84-85	33.11504800143637	35.0	34.0	36.0	27.0	37.0
86-87	32.870341896511626	35.0	34.0	35.0	27.0	37.0
88-89	32.70380577222243	35.0	33.0	35.0	26.5	36.0
90-91	32.52820257606302	35.0	33.0	35.0	26.0	36.0
92-93	32.38432539515178	35.0	33.0	35.0	25.5	36.0
94-95	32.24501699661755	35.0	33.0	35.0	25.0	35.5
96-97	32.11790748200624	35.0	33.0	35.0	25.0	35.0
98-99	32.05378848037827	35.0	33.0	35.0	25.0	35.0
100-101	30.887338514058207	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	85.0
8	283.0
9	240.0
10	401.0
11	869.0
12	1537.0
13	1782.0
14	1895.0
15	2016.0
16	2117.0
17	2298.0
18	2583.0
19	2921.0
20	3400.0
21	4182.0
22	4934.0
23	4872.0
24	4641.0
25	4894.0
26	5145.0
27	5451.0
28	6060.0
29	6950.0
30	8345.0
31	10555.0
32	13729.0
33	18939.0
34	29471.0
35	50930.0
36	103918.0
37	197122.0
38	255284.0
39	72004.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9475953757644	19.905116034328156	14.510657248268446	23.636631341639
2	15.192361222406856	20.788170691589418	39.57205396983387	24.447414116169863
3	18.44001884634804	27.137054161560435	29.23160161423068	25.191325377860842
4	12.429763180060515	18.123780683985824	40.057792114678065	29.388664021275595
5	13.735508289842638	38.470195970217056	34.523678981432056	13.27061675850825
6	28.559017003847586	38.170751839743765	19.988889839505177	13.28134131690347
7	26.126229257318307	32.06016356759063	23.60957305412528	18.20403412096579
8	25.354181553518558	34.31003131812053	21.5502168409307	18.785570287430215
9	26.031154239634834	16.360494582893004	21.00772288705112	36.60062829042104
10-11	24.89163978497863	25.868056601568195	28.68012016325429	20.560183450198885
12-13	26.0173569145084	23.316997461051177	28.48948812526284	22.17615749917758
14-15	23.134391330291542	24.9650113615131	25.97868736852891	25.92190993966645
16-17	22.995896346264928	27.465840067240592	26.579569037974775	22.958694548519706
18-19	22.776406379573025	27.12590158717914	27.59133994175843	22.506352091489404
20-21	24.280510819687546	25.762676312295103	27.037615901544186	22.919196966473162
22-23	23.88671778442038	26.463867690307925	26.615798110998433	23.03361641427326
24-25	23.213117085499206	26.33028694980397	26.966640834877015	23.48995512981981
26-27	23.09780676755785	26.868392798398787	27.16066713983258	22.87313329421079
28-29	23.046352987392016	26.9174365654421	27.077702438089773	22.95850800907611
30-31	23.393575628019295	26.187503840958414	27.189647547630898	23.229272983391393
32-33	23.159804355134714	26.313969279562727	27.32683754463043	23.19938882067213
34-35	23.514377535785684	26.26004523594631	27.018054613307367	23.207522614960638
36-37	23.106373336579523	26.34293972093225	27.421112197191484	23.129574745296743
38-39	23.136180027668384	26.514229454778583	27.051870990132894	23.297719527420142
40-41	23.66187847967029	26.125364536887517	27.187161071075653	23.025595912366537
42-43	23.411306148591205	26.433212165470355	27.108281651715	23.047200034223444
44-45	23.518335068741443	26.33445652802684	26.73438373729778	23.41282466593394
46-47	23.481541106991326	26.157981180207525	26.5606341226528	23.79984359014835
48-49	23.28217277143074	26.312704022673405	26.77126926956117	23.633853936334685
50-51	23.36152245348976	26.562622383478878	26.51731413677548	23.558541026255888
52-53	23.65325454197098	26.513156864139127	26.101044620187956	23.732543973701937
54-55	23.579929892718265	26.165271469903157	26.49935953901269	23.75543909836589
56-57	23.500654620208817	26.552275324885027	26.398877897214085	23.548192157692075
58-59	23.832735647865537	26.430031028221883	26.47829982940203	23.258933494510554
60-61	23.677293155875663	26.58050496613914	26.31782567351115	23.424376204474047
62-63	23.438791038254656	26.462461014599747	26.64904853023158	23.44969941691401
64-65	24.004395572599886	26.123155601582216	26.167702065533334	23.704746760284568
66-67	23.45085920758619	26.392461053032477	26.103085626682798	24.05359411269853
68-69	23.52052306924811	26.443929237194695	26.696016609810442	23.33953108374675
70-71	23.737062748306663	26.00289683577327	26.592446295870083	23.667594120049984
72-73	23.52010131695167	26.163765211701577	26.72638277515421	23.589750696192542
74-75	23.541490183414048	26.072967967310586	26.64203231586596	23.74350953340941
76-77	23.521969077121625	25.95282881315289	26.168464737290492	24.356737372434992
78-79	23.50164933369811	25.667718013953955	26.568192747270498	24.26243990507744
80-81	23.34749045223404	26.26508275735261	26.42664874168433	23.960778048729026
82-83	23.394284708466603	26.448263265574308	26.53615187730798	23.62130014865111
84-85	23.482238642253588	25.899519051120407	26.817781849302687	23.80046045732332
86-87	23.312297590253948	26.50159998649597	26.459821891480644	23.72628053176944
88-89	23.57240969468598	26.151700511759746	26.253919865089742	24.021969928464536
90-91	23.28494428652164	26.146051615251043	26.46700511284284	24.101998985384476
92-93	23.38140679961583	25.996727083863053	26.814594603062513	23.807271513458602
94-95	23.53636890552869	25.949756046421673	26.72565977121745	23.788215276832183
96-97	23.365619475798045	26.50707158100476	26.620281947435203	23.507026995761994
98-99	22.848136196138636	26.547666961494805	26.677869332491426	23.926327509875136
100-101	23.40259317528346	26.06225549587997	26.413196139773476	24.121955189063094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.5
3	3.0
4	4.0
5	4.5
6	5.0
7	12.5
8	21.5
9	22.5
10	28.5
11	41.5
12	53.0
13	56.5
14	65.0
15	79.0
16	85.0
17	105.5
18	122.0
19	139.0
20	186.5
21	222.5
22	272.5
23	355.5
24	467.5
25	696.0
26	998.0
27	1402.0
28	1919.0
29	2608.5
30	3712.0
31	5460.0
32	7955.5
33	11013.5
34	14421.0
35	17693.5
36	20789.5
37	23473.0
38	24900.0
39	25782.0
40	26584.0
41	27194.0
42	28080.5
43	29288.5
44	30515.5
45	31960.5
46	33655.5
47	34094.5
48	34646.5
49	36158.5
50	37707.5
51	36896.0
52	34505.0
53	33529.0
54	32872.0
55	31656.5
56	27401.5
57	22606.0
58	20272.5
59	18287.0
60	14982.5
61	11440.0
62	8961.5
63	6542.5
64	4195.0
65	2696.5
66	2123.0
67	1736.5
68	1262.5
69	816.0
70	525.5
71	385.0
72	275.0
73	208.0
74	173.0
75	126.5
76	76.5
77	55.5
78	43.0
79	31.5
80	22.0
81	18.0
82	14.5
83	6.5
84	5.5
85	6.0
86	7.0
87	5.5
88	3.0
89	4.0
90	3.5
91	3.0
92	2.0
93	4.0
94	6.0
95	3.0
96	2.0
97	4.0
98	3.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15424083984137293
2	0.0
3	2.4100131225214522E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03807820733583894
16-17	0.07350540023690429
18-19	0.026871646316114193
20-21	0.04115097406705379
22-23	0.06555235693258349
24-25	0.017352094482154454
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02169011810269307
38-39	0.005181528213421122
40-41	0.006145533462429704
42-43	0.003675270011845215
44-45	0.011447562331976897
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	6.0250328063036305E-5
58-59	0.017171343497965348
60-61	0.018316099731163035
62-63	0.028136903205437956
64-65	0.04820026245042904
66-67	0.018436600387289108
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01711109316990231
80-81	0.05886457051758647
82-83	0.04964627032394191
84-85	0.03145067124890495
86-87	0.058924820845649505
88-89	0.03404143535561551
90-91	0.0
92-93	0.003615019683782178
94-95	0.0
96-97	0.0
98-99	0.0010845059051346534
100-101	0.046513253264664024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	829871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.60813661555392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6405789942098	51.45429407799721
2	13.192791133950402	17.047233038454728
3	3.7681048249344	7.3035069393326895
4	1.4239828218077772	3.6800350675823545
5	0.6376621337036958	2.0599081124446994
6	0.3497759135556454	1.3559022004699992
7	0.20391325693359472	0.9222118893181765
8	0.1252526537752111	0.6473872453255612
9	0.10045283530006528	0.5841063455837721
>10	0.4547566981290721	5.491289400181777
>50	0.0531500555483207	2.4486123071395016
>100	0.04657390980422303	5.5644436815035485
>500	0.002441374282623302	1.0354541360848397
>1k	5.633940652207619E-4	0.40561555858115994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1217	0.14664929850543035	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1068	0.12869470074264555	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1058	0.1274896941813848	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	935	0.11266811347787789	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	922	0.11110160494823894	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	848	0.10218455639490957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	6.0250328063036305E-5	0.0
22-23	0.0	0.0	0.0	3.012516403151815E-4	0.0
24-25	0.0	0.0	0.0	4.8200262450429044E-4	0.0
26-27	0.0	0.0	0.0	6.02503280630363E-4	0.0
28-29	0.0	0.0	0.0	6.627536086933994E-4	0.0
30-31	0.0	0.0	0.0	0.001807509841891089	0.0
32-33	0.0	0.0	0.0	0.004097022308286468	0.0
34-35	0.0	0.0	0.0	0.007591541335942574	0.0
36-37	0.0	0.0	0.0	0.013255072173867986	0.0
38-39	0.0	0.0	0.0	0.02560638942679043	0.0
40-41	0.0	0.0	0.0	0.04229573030025148	0.0
42-43	0.0	0.0	0.0	0.057599313628262705	0.0
44-45	0.0	0.0	0.0	0.07597566368748879	0.0
46-47	0.0	0.0	0.0	0.09513526801153432	0.0
48-49	0.0	0.0	0.0	0.11574088020909273	0.0
50-51	0.0	0.0	0.0	0.1418895225884505	0.0
52-53	0.0	0.0	0.0	0.1694841728413211	0.0
54-55	0.0	0.0	0.0	0.20021184015346963	0.0
56-57	0.0	0.0	0.0	0.23377127288458086	0.0
58-59	0.0	0.0	0.0	0.27606700318483235	0.0
60-61	0.0	0.0	0.0	0.320230493655038	0.0
62-63	0.0	0.0	0.0	0.37132277185249274	0.0
64-65	0.0	0.0	0.0	0.4196435349590478	0.0
66-67	0.0	0.0	0.0	0.47778510153987785	0.0
68-69	0.0	0.0	0.0	0.542614454535705	0.0
70-71	0.0	0.0	0.0	0.6237113961085519	0.0
72-73	0.0	0.0	0.0	0.7125806300015304	0.0
74-75	0.0	0.0	0.0	0.799943605692933	0.0
76-77	0.0	0.0	0.0	0.889475593194605	0.0
78-79	0.0	0.0	0.0	0.9917203999175775	0.0
80-81	0.0	0.0	0.0	1.122102109845988	0.0
82-83	0.0	0.0	0.0	1.2598343597980892	0.0
84-85	0.0	0.0	0.0	1.4009406281217203	0.0
86-87	0.0	0.0	0.0	1.5554827196034082	0.0
88-89	0.0	0.0	0.0	1.7320161808281047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	680	0.0	51.032078	1
CCGTGCG	265	0.0	50.197216	9
GTATCAA	1365	0.0	46.31784	1
ACCGTGC	320	0.0	46.00404	8
AAACCGT	280	0.0	44.09604	6
AACCGTG	320	0.0	40.068035	7
ATCAACG	1785	0.0	35.383244	3
TCAACGC	1865	0.0	33.865463	4
GCGTTAT	60	0.003935403	31.691154	1
TATCAAC	2065	0.0	30.125584	2
CGCAGAG	2165	0.0	29.392134	8
TGCGCGG	215	0.0	28.727547	12-13
ACGCAGA	2225	0.0	28.59954	7
CAACGCA	2375	0.0	26.593304	5
AACGCAG	2440	0.0	26.27412	6
GTCTAAT	575	0.0	24.801773	1
GTACATG	3095	0.0	24.574722	1
CCCATAA	705	0.0	24.249212	2
TACATGG	3040	0.0	24.056442	2
GCCCATA	715	0.0	23.934578	1
>>END_MODULE