FastQCFastQC Report
Fri 10 Feb 2017
SRR3224558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829871
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC11000.13255072173867988No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT10240.12339267187309835No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT10060.12122366006282903No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG8830.1064020793593221No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG8740.10531757345418745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG2450.052.335859
AACCGTG2900.047.4899377
AAACCGT2900.047.4899376
ACCGTGC2850.046.6567848
GGTATCA9400.045.1352041
GTATCAA16500.044.2039151
ATCAACG20950.034.682393
TCAACGC21600.033.4188464
TATCAAC23900.030.7989162
CAACGCA24250.029.7668885
CGTATAC650.005741491429.3359321
GTATACG650.005741491429.3359321
TGCGCGG2350.029.302312-13
CGCAGAG25700.028.4570068
AACGCAG25450.028.3633426
ACGCAGA26200.027.9139337
CGGTCTA2450.027.14119528-29
CGGCTTA2650.026.88190816-17
AAAACCG7800.026.7891965
GCGCGGC2850.026.66101812-13
Produced by FastQC (version 0.11.5)