##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	829871
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13284715335275	34.0	33.0	34.0	31.0	34.0
2	33.26820554037917	34.0	33.0	34.0	31.0	34.0
3	33.38643114411758	34.0	34.0	34.0	31.0	34.0
4	36.61170109571247	37.0	37.0	37.0	35.0	37.0
5	36.64428206311583	37.0	37.0	37.0	35.0	37.0
6	36.67330585115036	37.0	37.0	37.0	35.0	37.0
7	36.64754763089685	37.0	37.0	37.0	35.0	37.0
8	36.6330104317418	37.0	37.0	37.0	35.0	37.0
9	38.466494190060864	39.0	39.0	39.0	37.0	39.0
10-11	38.49198007883153	39.0	39.0	39.0	37.0	39.0
12-13	38.489066372966406	39.0	39.0	39.0	37.0	39.0
14-15	40.077060772095905	41.0	40.0	41.0	38.0	41.0
16-17	40.0878015980797	41.0	40.0	41.0	38.0	41.0
18-19	40.0813536079704	41.0	40.0	41.0	38.0	41.0
20-21	40.088718608072824	41.0	40.0	41.0	38.0	41.0
22-23	40.006857089836856	41.0	40.0	41.0	38.0	41.0
24-25	39.96298340344463	41.0	40.0	41.0	38.0	41.0
26-27	39.90339402148045	41.0	40.0	41.0	38.0	41.0
28-29	39.87107634801072	41.0	40.0	41.0	38.0	41.0
30-31	39.80134563082696	41.0	40.0	41.0	38.0	41.0
32-33	39.74502000913395	41.0	40.0	41.0	37.0	41.0
34-35	39.66356397560585	41.0	40.0	41.0	37.0	41.0
36-37	39.55793490795557	41.0	40.0	41.0	37.0	41.0
38-39	39.46153438305471	41.0	40.0	41.0	36.5	41.0
40-41	39.33571784048364	41.0	39.0	41.0	36.0	41.0
42-43	39.24592255904834	41.0	39.0	41.0	35.5	41.0
44-45	39.017627438481405	41.0	39.0	41.0	35.0	41.0
46-47	38.79690216913231	40.5	39.0	41.0	35.0	41.0
48-49	38.71030798762699	40.0	38.0	41.0	35.0	41.0
50-51	38.57390726992509	40.0	38.0	41.0	35.0	41.0
52-53	38.36797225110891	40.0	37.5	41.0	35.0	41.0
54-55	38.203479215444325	40.0	37.0	41.0	34.5	41.0
56-57	38.00848445119784	40.0	37.0	41.0	34.0	41.0
58-59	37.769420789496195	40.0	36.0	41.0	34.0	41.0
60-61	37.5338739394436	39.0	35.5	41.0	34.0	41.0
62-63	37.31973583846164	39.0	35.0	41.0	33.5	41.0
64-65	37.04084670991034	39.0	35.0	41.0	33.0	41.0
66-67	36.733414590942445	38.0	35.0	40.5	33.0	41.0
68-69	36.449097510335946	37.0	35.0	40.0	33.0	41.0
70-71	36.12870253328529	37.0	35.0	39.5	32.5	41.0
72-73	35.78510635990413	36.0	35.0	39.0	32.0	41.0
74-75	35.43798373482144	36.0	35.0	39.0	32.0	40.5
76-77	33.56568370264776	34.5	32.5	36.5	28.5	39.0
78-79	34.84893917247379	35.0	34.5	37.0	31.5	39.0
80-81	34.7484524703237	35.0	35.0	37.0	32.0	39.0
82-83	34.51063358039984	35.0	35.0	36.0	32.0	38.0
84-85	34.27669782411965	35.0	35.0	36.0	32.0	37.0
86-87	34.05523750076819	35.0	35.0	36.0	32.0	37.0
88-89	33.885042976559006	35.0	34.0	35.0	31.0	36.5
90-91	33.73399239158857	35.0	34.0	35.0	31.0	36.0
92-93	33.626896228449965	35.0	34.0	35.0	31.0	36.0
94-95	33.5222528561668	35.0	34.0	35.0	31.0	36.0
96-97	33.469813983137136	35.0	34.0	35.0	31.0	35.5
98-99	33.42488712101037	35.0	34.0	35.0	31.0	35.0
100-101	32.601955002644985	34.5	33.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	3.0
14	13.0
15	42.0
16	137.0
17	317.0
18	542.0
19	898.0
20	1314.0
21	2040.0
22	2712.0
23	2616.0
24	2239.0
25	2377.0
26	3062.0
27	3859.0
28	4852.0
29	5701.0
30	6402.0
31	7495.0
32	9164.0
33	12421.0
34	20038.0
35	37470.0
36	93810.0
37	209525.0
38	304922.0
39	95872.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19560157996324	20.02477471477595	14.484005415079398	23.295618290181412
2	15.091140671260955	20.89083725060883	40.00513332795097	24.012888750179247
3	18.30766468523421	27.50053924043616	29.474821990405736	24.716974083923887
4	12.368669347404596	18.23379778302893	40.46544583435257	28.932087035213904
5	13.648506816119612	38.163762801688456	34.91554711515404	13.27218326703789
6	28.50141769021932	37.9527661528117	20.281706433891532	13.264109723077441
7	26.041878798030055	32.052451525598556	23.785142510100968	18.12052716627042
8	25.441544529209963	34.24206894806542	21.524670701831973	18.791715820892644
9	26.144424856393343	16.347842013999767	21.163650736078257	36.34408239352863
10-11	24.98032826788742	25.95487732430703	28.753746064147318	20.311048343658232
12-13	26.05447111659523	23.59432972112533	28.565765040590644	21.785434121688795
14-15	23.207884116929016	25.144329660875	26.2939661706458	25.35382005155018
16-17	22.725278989144098	27.65700934241587	26.775486792525587	22.84222487591445
18-19	22.633216487863777	27.112948880006655	28.15009802728376	22.103736604845814
20-21	24.112652600857253	25.83691726686758	27.59546478265637	22.454965349618792
22-23	23.81504109126841	26.416262984117804	27.06058853300557	22.708107391608223
24-25	23.365898772996893	26.215088553293146	27.280077843752164	23.138934829957794
26-27	23.07700650301773	26.958572408960464	27.337859878736463	22.62656120928534
28-29	23.013596777167344	26.80130484714321	27.586526862763293	22.598571512926156
30-31	23.275695340257766	26.127188387005816	27.528442930674746	23.068673342061675
32-33	23.19080096882644	26.2753804812802	27.762119368093796	22.77169918179956
34-35	23.54777115733039	26.377123686412922	27.21554185673925	22.859563299517436
36-37	23.121064431407305	26.351521928923425	27.492147691484558	23.035265948184705
38-39	23.10829032464082	26.514663122340703	27.14903882651641	23.22800772650207
40-41	23.440209381940086	26.35222823788276	27.369916529195503	22.83764585098166
42-43	23.256265130363634	26.39524697211976	27.464871046222843	22.883616851293755
44-45	23.532881777925603	26.347782021779985	27.1617145608241	22.957621639470304
46-47	23.391491963640707	26.154137455693593	26.858818889755888	23.595551690909815
48-49	23.24371613145784	26.06641096180936	27.169303848948562	23.52056905778424
50-51	23.517570803173022	26.247754169021448	26.879177607122074	23.355497420683456
52-53	23.652063785855745	26.4047221825574	26.49527848019661	23.44793555139025
54-55	23.636384450113333	26.058086136279012	26.869838806272302	23.435690607335356
56-57	23.662150729933167	26.455588200558687	26.414735081380858	23.46752598812729
58-59	23.858716453445446	26.224673387279058	26.77696909422419	23.139641065051308
60-61	23.57993069157752	26.490583094771736	26.676329667018233	23.253156546632514
62-63	23.41749500826032	26.301858963622056	26.857728490331628	23.42291753778599
64-65	23.759897622642555	26.170452998116573	26.571177930063826	23.498471449177043
66-67	23.490156903904342	26.231004577819927	26.58129998517842	23.697538533097312
68-69	23.711637109864064	26.30788399642836	26.77175127218568	23.2087276215219
70-71	23.950830912274316	26.059592394480585	26.641128560945017	23.34844813230008
72-73	23.560288285769715	26.120505476152317	26.813745750845612	23.50546048723235
74-75	23.72049390808933	25.884203689489087	26.739216095031637	23.65608630738994
76-77	23.564300480017543	25.81436389390811	26.5631599337483	24.058175692326046
78-79	23.491447357321384	25.720834608737984	26.67353175817846	24.11418627576217
80-81	23.33850915771442	26.20389132817438	26.618800835603405	23.838798678507796
82-83	23.39821741983179	26.278205658875635	26.804375895100947	23.519201026191624
84-85	23.582437035193774	25.894726567560223	26.90864657826097	23.614189818985032
86-87	23.233008503731302	26.438265706356773	26.725057267936826	23.603668521975102
88-89	23.593847718500825	26.043324805903566	26.276614076163646	24.086213399431962
90-91	23.539803174228283	26.187503840958414	26.402416761159266	23.870276223654038
92-93	23.627013869642234	26.109149625845014	26.680624676154096	23.583211828358657
94-95	23.52937986747338	26.01663391057164	26.57238293662509	23.881603285329888
96-97	23.303079635268613	26.49538301736053	26.710898440842012	23.490638906528847
98-99	22.644965301836066	26.676857005486394	26.83983414289691	23.838343549780628
100-101	23.328632573192884	26.149872452440228	26.65656576009724	23.86492921426965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.5
2	4.0
3	6.5
4	4.0
5	3.0
6	7.5
7	13.5
8	21.0
9	31.0
10	40.0
11	45.0
12	52.0
13	75.5
14	93.0
15	104.5
16	113.0
17	116.5
18	138.5
19	160.0
20	198.5
21	257.0
22	319.0
23	426.5
24	604.0
25	830.0
26	1132.5
27	1574.5
28	2121.5
29	2949.0
30	4273.0
31	6225.0
32	8817.0
33	11916.0
34	15152.0
35	18074.0
36	21023.0
37	23520.5
38	24745.5
39	25497.5
40	26294.0
41	27154.0
42	28041.0
43	29296.0
44	30805.0
45	32087.0
46	33003.0
47	34056.5
48	35354.0
49	36768.0
50	38143.0
51	36696.5
52	33813.5
53	32892.0
54	32663.0
55	30919.0
56	26400.0
57	22201.5
58	20338.0
59	18725.0
60	15176.5
61	11154.5
62	8514.5
63	5989.0
64	3807.0
65	2444.5
66	1826.5
67	1451.0
68	1058.5
69	689.0
70	415.0
71	291.0
72	209.0
73	150.5
74	126.5
75	91.0
76	56.5
77	34.0
78	23.0
79	13.0
80	5.0
81	2.5
82	0.5
83	1.0
84	0.5
85	1.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4854971435319465
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.001205006561260726
22-23	0.0013255072173867985
24-25	4.217522964412541E-4
26-27	0.003072766731214852
28-29	0.00506102755729505
30-31	9.037549209455445E-4
32-33	1.2050065612607261E-4
34-35	0.004157272636349505
36-37	0.0023497627944584158
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008736297569140263
46-47	0.08459146060050297
48-49	9.640052490085809E-4
50-51	0.0
52-53	6.0250328063036305E-5
54-55	0.0
56-57	0.00831454527269901
58-59	0.04018696881804521
60-61	0.029643161407013858
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	6.627536086933993E-4
78-79	0.00831454527269901
80-81	0.006205783790492738
82-83	7.832542648194718E-4
84-85	0.002711264762836634
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.2050065612607261E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06898662563217657
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	829871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.040297152691515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63822109559484	49.57376825425354
2	13.753236740547143	17.340162618708128
3	3.9335967140727104	7.439253172019838
4	1.5121966812584653	3.8131731255939036
5	0.6955552841611004	2.192400589982028
6	0.3804700997120203	1.439096888613596
7	0.244585997834293	1.0793141788002976
8	0.1484730790488143	0.7487829617929849
9	0.10261094059725623	0.5821761767731389
>10	0.4808190128534648	5.719203093803447
>50	0.0557897138178326	2.506466824631544
>100	0.05080221739402865	5.897714788284157
>500	0.003067303669934174	1.2902195205210032
>1k	5.751194381126577E-4	0.3782678062224373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1100	0.13255072173867988	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1024	0.12339267187309835	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1006	0.12122366006282903	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	883	0.1064020793593221	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	874	0.10531757345418745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.8075098418910893E-4	0.0
12-13	0.0	0.0	0.0	2.4100131225214522E-4	0.0
14-15	0.0	0.0	0.0	2.4100131225214522E-4	0.0
16-17	0.0	0.0	0.0	2.4100131225214522E-4	0.0
18-19	0.0	0.0	0.0	2.4100131225214522E-4	0.0
20-21	0.0	0.0	0.0	3.012516403151815E-4	0.0
22-23	0.0	0.0	0.0	4.217522964412541E-4	0.0
24-25	0.0	0.0	0.0	6.627536086933994E-4	0.0
26-27	0.0	0.0	0.0	8.435045928825083E-4	0.0
28-29	0.0	0.0	0.0	9.037549209455445E-4	0.0
30-31	0.0	0.0	0.0	0.002048511154143234	0.0
32-33	0.0	0.0	0.0	0.004338023620538613	0.0
34-35	0.0	0.0	0.0	0.007712041992068647	0.0
36-37	0.0	0.0	0.0	0.013496073486120131	0.0
38-39	0.0	0.0	0.0	0.026028141723231684	0.0
40-41	0.0	0.0	0.0	0.04319948522119703	0.0
42-43	0.0	0.0	0.0	0.05880432018952343	0.0
44-45	0.0	0.0	0.0	0.07669866762424521	0.0
46-47	0.0	0.0	0.0	0.09597877260441683	0.0
48-49	0.0	0.0	0.0	0.11712663775454257	0.0
50-51	0.0	0.0	0.0	0.1436970324303416	0.0
52-53	0.0	0.0	0.0	0.1718941859638426	0.0
54-55	0.0	0.0	0.0	0.2028026042601802	0.0
56-57	0.0	0.0	0.0	0.23672353895966963	0.0
58-59	0.0	0.0	0.0	0.2795615222124884	0.0
60-61	0.0	0.0	0.0	0.32438776629138744	0.0
62-63	0.0	0.0	0.0	0.3759620471133465	0.0
64-65	0.0	0.0	0.0	0.424825063172469	0.0
66-67	0.0	0.0	0.0	0.48350888270586634	0.0
68-69	0.0	0.0	0.0	0.5489407389823238	0.0
70-71	0.0	0.0	0.0	0.6300376805551706	0.0
72-73	0.0	0.0	0.0	0.7194491674007165	0.0
74-75	0.0	0.0	0.0	0.8075351470288756	0.0
76-77	0.0	0.0	0.0	0.8977901384673039	0.0
78-79	0.0	0.0	0.0	1.0014207027357265	0.0
80-81	0.0	0.0	0.0	1.1330074192253976	0.0
82-83	0.0	0.0	0.0	1.2733906836122721	0.0
84-85	0.0	0.0	0.0	1.4160032101374793	0.0
86-87	0.0	0.0	0.0	1.5721118101488063	0.0
88-89	0.0	0.0	0.0	1.7501515295750785	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	245	0.0	52.33585	9
AACCGTG	290	0.0	47.489937	7
AAACCGT	290	0.0	47.489937	6
ACCGTGC	285	0.0	46.656784	8
GGTATCA	940	0.0	45.135204	1
GTATCAA	1650	0.0	44.203915	1
ATCAACG	2095	0.0	34.68239	3
TCAACGC	2160	0.0	33.418846	4
TATCAAC	2390	0.0	30.798916	2
CAACGCA	2425	0.0	29.766888	5
CGTATAC	65	0.0057414914	29.335932	1
GTATACG	65	0.0057414914	29.335932	1
TGCGCGG	235	0.0	29.3023	12-13
CGCAGAG	2570	0.0	28.457006	8
AACGCAG	2545	0.0	28.363342	6
ACGCAGA	2620	0.0	27.913933	7
CGGTCTA	245	0.0	27.141195	28-29
CGGCTTA	265	0.0	26.881908	16-17
AAAACCG	780	0.0	26.789196	5
GCGCGGC	285	0.0	26.661018	12-13
>>END_MODULE