##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224557_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	998234
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20948795572982	34.0	31.0	34.0	31.0	34.0
2	32.370918041260865	34.0	31.0	34.0	31.0	34.0
3	32.51717633340479	34.0	31.0	34.0	31.0	34.0
4	35.80587116848354	37.0	37.0	37.0	35.0	37.0
5	35.81062656651647	37.0	37.0	37.0	35.0	37.0
6	35.911483680179195	37.0	37.0	37.0	35.0	37.0
7	35.87650791297431	37.0	37.0	37.0	35.0	37.0
8	35.91428162134329	37.0	37.0	37.0	35.0	37.0
9	37.63946729925048	39.0	39.0	39.0	35.0	39.0
10-11	37.71458295349588	39.0	39.0	39.0	35.0	39.0
12-13	37.70241045686683	39.0	39.0	39.0	35.0	39.0
14-15	39.182478757485725	41.0	40.0	41.0	36.0	41.0
16-17	39.01864142074905	41.0	39.5	41.0	36.0	41.0
18-19	39.091541662576105	41.0	39.5	41.0	36.0	41.0
20-21	39.15513897543061	41.0	40.0	41.0	36.0	41.0
22-23	39.10774477727667	41.0	40.0	41.0	36.0	41.0
24-25	39.032750837979876	41.0	39.5	41.0	35.5	41.0
26-27	38.84554373022758	41.0	39.0	41.0	35.0	41.0
28-29	38.77498011488288	41.0	39.0	41.0	35.0	41.0
30-31	38.70971886351296	41.0	39.0	41.0	35.0	41.0
32-33	38.518946960331945	40.5	39.0	41.0	35.0	41.0
34-35	38.505024873927354	40.0	38.0	41.0	35.0	41.0
36-37	38.49221174594334	40.0	38.0	41.0	35.0	41.0
38-39	38.35306050485157	40.0	38.0	41.0	34.0	41.0
40-41	38.24531873288227	40.0	38.0	41.0	34.0	41.0
42-43	38.08438151776036	40.0	38.0	41.0	33.5	41.0
44-45	37.87887359076129	40.0	37.0	41.0	33.0	41.0
46-47	37.674868818333174	40.0	37.0	41.0	33.0	41.0
48-49	37.42805093795643	40.0	36.0	41.0	32.5	41.0
50-51	36.10038277598238	38.5	34.5	40.0	30.0	40.5
52-53	36.38959252039101	39.0	35.0	40.0	31.0	40.5
54-55	36.78119759495269	39.0	35.0	41.0	32.0	41.0
56-57	36.5863640188573	38.5	35.0	41.0	31.0	41.0
58-59	36.39874718753319	37.5	35.0	41.0	31.0	41.0
60-61	36.119125876297545	37.0	35.0	41.0	31.0	41.0
62-63	35.82938419248393	36.5	35.0	40.0	31.0	41.0
64-65	35.57745678868882	36.0	35.0	40.0	30.0	41.0
66-67	35.34123462033952	35.5	35.0	39.5	30.0	41.0
68-69	35.05306421139733	35.0	35.0	39.0	30.0	41.0
70-71	34.789258831095715	35.0	34.5	39.0	29.5	41.0
72-73	34.49732677909188	35.0	34.0	37.5	29.0	40.5
74-75	34.24847881358479	35.0	34.0	37.0	29.0	39.5
76-77	33.96533177591627	35.0	34.0	37.0	29.0	39.0
78-79	33.76919539907477	35.0	34.0	36.0	29.0	39.0
80-81	33.502113231967655	35.0	34.0	36.0	29.0	38.0
82-83	33.28008813564755	35.0	34.0	35.5	28.5	37.0
84-85	33.07900101579389	35.0	34.0	35.0	27.5	37.0
86-87	32.880583610656416	35.0	33.5	35.0	27.0	36.0
88-89	32.75388686420218	35.0	33.0	35.0	27.0	36.0
90-91	32.59265212365037	35.0	33.0	35.0	27.0	36.0
92-93	32.46340988185135	35.0	33.0	35.0	26.5	35.5
94-95	32.340535886375335	35.0	33.0	35.0	26.0	35.0
96-97	32.21771548554747	35.0	33.0	35.0	25.0	35.0
98-99	32.16733351098039	35.0	33.0	35.0	25.0	35.0
100-101	30.891841492075006	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	117.0
8	486.0
9	438.0
10	547.0
11	1069.0
12	1619.0
13	1977.0
14	1926.0
15	2077.0
16	2160.0
17	2423.0
18	2585.0
19	2927.0
20	3414.0
21	4424.0
22	5236.0
23	5296.0
24	5177.0
25	5549.0
26	5964.0
27	6177.0
28	7009.0
29	7925.0
30	10099.0
31	12803.0
32	17101.0
33	24490.0
34	41958.0
35	77711.0
36	170375.0
37	261224.0
38	238979.0
39	66963.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.44013038492447	18.07681233138799	12.910174257906517	24.57288302578102
2	16.865484445530807	18.800501685977437	37.429199967141976	26.904813901349783
3	20.83279070839102	25.157428017879575	27.37544503593346	26.634336237795946
4	13.349274819330939	15.607663133093041	37.08378997309248	33.959272074483536
5	14.232133948553145	38.79080456085447	33.07290675332638	13.904154737266012
6	31.57496138180026	35.5434697676096	18.463807083309124	14.417761767281018
7	28.543407657923893	30.375943916957347	21.033645417807847	20.047003007310913
8	27.099157111458837	33.786266546721514	19.571863911668004	19.54271243015165
9	27.57539815313844	14.838104091826166	18.860808187258698	38.7256895677767
10-11	27.5448441948481	23.955154803382776	26.216147716867987	22.283853284901138
12-13	27.445368520807744	21.759026440694264	26.665040461454932	24.13056457704306
14-15	24.47716711712118	23.881955864476826	23.803740125325564	27.83713689307643
16-17	24.46037720338979	26.701222174270235	25.00647853112527	23.83192209121471
18-19	24.43856815339725	26.0039740116074	25.543094879276623	24.01436295571873
20-21	25.580197424462593	24.201683619782532	25.32028862053415	24.897830335220725
22-23	25.712184956079547	25.155748953236724	24.493759516599354	24.638306574084375
24-25	24.90068242321862	24.953134127072392	25.113094289827977	25.033089159881012
26-27	24.581460859878547	26.26403228100826	25.29006224993338	23.86444460917981
28-29	24.18581214424674	26.31572356782077	25.540404354089326	23.958059933843167
30-31	24.327312033050365	25.2381205208398	25.559838675100227	24.8747287710096
32-33	24.232394408525458	25.567001324338783	25.75192790467967	24.448676362456098
34-35	25.15772854861686	24.614318887154717	25.29396914951805	24.93398341471038
36-37	23.922460976737593	25.105245201404973	26.131821149037627	24.84047267281981
38-39	24.272490979864926	25.569747446236086	25.046446870740702	25.11131470315829
40-41	24.920041696032715	24.96667673502632	25.71829731515418	24.39498425378678
42-43	24.71463634542176	25.33505309557203	25.733119615307555	24.217190943698657
44-45	24.770119203387146	24.73906100031459	25.445735307967134	25.04508448833113
46-47	24.717050310848958	24.641016034316603	25.481099621932334	25.16083403290211
48-49	23.99136875722526	25.341402917552397	25.737853048483622	24.92937527673872
50-51	24.337429901205528	25.52021870623521	25.424549754867094	24.717801637692162
52-53	24.48959863118267	25.4905162517005	24.696914751451065	25.322970365665764
54-55	24.654439740581868	24.66105141680207	25.24453184323515	25.43997699938091
56-57	24.61707375224647	25.098123285722586	25.258456434062555	25.02634652796839
58-59	24.85768890692356	25.284559565765186	25.60176940167472	24.255982125636535
60-61	24.50487820151544	25.755000313106645	25.113607614753587	24.626513870624333
62-63	23.959286843377598	25.110187558087393	25.903558017250234	25.026967581284776
64-65	25.074253344845715	24.356650579068347	25.060823348949878	25.50827272713606
66-67	24.064156656383354	25.244349771606228	24.78220181813263	25.909291753877785
68-69	24.300965505081976	25.39895455374191	26.05386111873569	24.24621882244043
70-71	24.910892636395875	24.720556502783918	25.670784605613512	24.697766255206695
72-73	24.26695544331289	25.23246052528766	26.056766249196077	24.44381778220337
74-75	24.519150820348738	24.94996163224254	25.927538032164804	24.60334951524392
76-77	24.414666300687013	24.2614958015856	25.40321207252007	25.920625825207317
78-79	24.60342071395042	23.856326206720695	25.985593144344588	25.554659934984294
80-81	24.314761440104803	25.24583689791197	25.13883689239909	25.300564769584135
82-83	23.684300201050757	25.628058106274267	25.705130903485614	24.982510789189362
84-85	24.293304941441722	24.284737270620653	26.364627043276755	25.05733074466086
86-87	24.152587605414418	25.879161385609162	25.377241661692572	24.591009347283848
88-89	24.558000779630206	24.69714172334351	24.960241865741263	25.784615631285018
90-91	24.07691984043821	24.605052522755187	25.37170643356167	25.94632120324493
92-93	24.00442195288226	24.840926406141445	26.00371768625874	25.15093395471756
94-95	24.363976782998776	24.39392967981455	26.051406784381214	25.19068675280545
96-97	24.86626382190949	24.912946263100636	25.827962181212023	24.392827733777853
98-99	23.384904976142607	25.26559696580824	25.960030975042653	25.3894670830065
100-101	24.31650696196252	24.682369542754458	25.252535882194355	25.74858761308867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	2.0
4	2.0
5	1.5
6	3.0
7	8.0
8	13.0
9	17.5
10	17.5
11	16.0
12	24.0
13	26.5
14	20.5
15	27.5
16	37.5
17	39.5
18	43.5
19	47.5
20	52.5
21	74.5
22	94.0
23	114.5
24	154.0
25	194.0
26	256.5
27	320.5
28	393.0
29	531.0
30	768.5
31	1358.5
32	2523.0
33	4526.5
34	7486.5
35	11804.0
36	18063.5
37	24317.5
38	28281.5
39	29865.0
40	29973.0
41	29284.5
42	28311.0
43	27586.5
44	27102.5
45	28539.5
46	31930.5
47	32823.5
48	34183.5
49	38785.0
50	43949.5
51	45521.5
52	45564.5
53	48165.5
54	52113.5
55	52874.5
56	44896.0
57	38136.0
58	36392.5
59	35217.0
60	30209.5
61	22931.0
62	18972.0
63	13728.5
64	8120.5
65	5165.0
66	4031.0
67	3608.0
68	2728.5
69	1713.0
70	1093.0
71	748.0
72	603.0
73	524.5
74	384.0
75	253.5
76	157.5
77	93.0
78	66.0
79	44.5
80	30.0
81	21.5
82	17.0
83	17.0
84	15.0
85	8.5
86	5.0
87	7.0
88	9.5
89	7.0
90	6.0
91	6.0
92	5.0
93	4.5
94	4.0
95	3.0
96	4.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1495641302540286
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03536245008685338
16-17	0.07097534245477513
18-19	0.025044228106836673
20-21	0.03846793437210113
22-23	0.06361233939136515
24-25	0.01733060584993098
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02078670932867444
38-39	0.005058934077581009
40-41	0.005760172464572434
42-43	0.0034060150225297876
44-45	0.010819106542153444
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015677686794879755
60-61	0.017180340481289958
62-63	0.027698916286161362
64-65	0.046832706559784575
66-67	0.017731313499640362
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01677963283158057
80-81	0.05715092853980129
82-83	0.047684210315417024
84-85	0.029702454534708295
86-87	0.05654986706523721
88-89	0.032557496538887676
90-91	0.0
92-93	0.003255749653888768
94-95	0.0
96-97	0.0
98-99	0.0011019460367008137
100-101	0.04558049515444275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	998234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.91971556930246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1046953137503	28.686641923112738
2	15.21018960737466	12.447932649778627
3	5.930587246452031	7.280338298512494
4	2.9727540245837605	4.865769965738666
5	1.596651526710959	3.2667263168152485
6	0.9871870647197131	2.4237248341215145
7	0.6535023628656824	1.8718791568631568
8	0.425025235617396	1.3913529400991684
9	0.30609889017646796	1.1272931569890199
>10	1.5159076351460987	11.020226489148808
>50	0.1318490868120969	3.7997670887826094
>100	0.14101660374588398	12.526750326562281
>500	0.01858742579173075	5.236220780331274
>1k	0.00594797625335384	4.055376073144449
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	4217	0.42244603970612105	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2911	0.2916149920760062	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2459	0.24633502765884552	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	2259	0.22629964517337617	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	2193	0.2196879689531713	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1783	0.17861543485795914	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1726	0.1729053508496004	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1716	0.17190358172532694	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1672	0.16749579757852368	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1621	0.162386775044729	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1586	0.15888058310977185	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1500	0.15026536864102005	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1356	0.13583989325148213	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1317	0.1319329936668156	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1313	0.1315322860171062	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1291	0.12932839394370457	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1263	0.12652344039573887	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1189	0.11911034887611523	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1181	0.11830893357669643	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1142	0.11440203399202993	No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT	1140	0.11420168016717522	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	1113	0.11149690353163687	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1023	0.10248098141317566	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1018	0.10198009685103893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.0017691242734669E-4	0.0
18-19	0.0	0.0	0.0	1.0017691242734669E-4	0.0
20-21	0.0	0.0	0.0	2.0035382485469338E-4	0.0
22-23	0.0	0.0	0.0	4.0070764970938675E-4	0.0
24-25	0.0	0.0	0.0	8.515037556324469E-4	0.0
26-27	0.0	0.0	0.0	0.0018031844236922404	0.0
28-29	0.0	0.0	0.0	0.003906899584666521	0.0
30-31	0.0	0.0	0.0	0.009667072049238955	0.0
32-33	0.0	0.0	0.0	0.020436090135178726	0.0
34-35	0.0	0.0	0.0	0.03401006176908421	0.0
36-37	0.0	0.0	0.0	0.06120809349310882	0.0
38-39	0.0	0.0	0.0	0.10683867710376524	0.0
40-41	0.0	0.0	0.0	0.16569261315483144	0.0
42-43	0.0	0.0	0.0	0.21823540372297479	0.0
44-45	0.0	0.0	0.0	0.2767888090367589	0.0
46-47	0.0	0.0	0.0	0.3484153014223118	0.0
48-49	0.0	0.0	0.0	0.42740479687127464	0.0
50-51	0.0	0.0	0.0	0.5191167601985105	0.0
52-53	0.0	0.0	0.0	0.6059701432730201	0.0
54-55	0.0	0.0	0.0	0.6905695458179144	0.0
56-57	0.0	0.0	0.0	0.7863887625546715	0.0
58-59	0.0	0.0	0.0	0.8985869044732999	0.0
60-61	0.0	0.0	0.0	1.0279653868732181	0.0
62-63	0.0	0.0	0.0	1.1667104105850932	0.0
64-65	0.0	0.0	0.0	1.2967400429157894	0.0
66-67	0.0	0.0	0.0	1.4468050577319547	0.0
68-69	0.0	0.0	0.0	1.6181075779827174	0.0
70-71	0.0	0.0	0.0	1.8112987535988556	0.0
72-73	0.0	0.0	0.0	2.0186148738672496	0.0
74-75	0.0	0.0	0.0	2.220471352408353	0.0
76-77	0.0	0.0	0.0	2.435100387283944	0.0
78-79	0.0	0.0	0.0	2.681435414942789	0.0
80-81	0.0	0.0	0.0	2.9549183858694454	0.0
82-83	0.0	0.0	0.0	3.2507408082674	0.0
84-85	0.0	0.0	0.0	3.5474648228772008	0.0
86-87	0.0	0.0	0.0	3.8590651089824632	0.0
88-89	0.0	0.0	0.0	4.208983064091185	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	800	0.0	49.331722	1
GTATCAA	1635	0.0	45.658333	1
ATCAACG	2150	0.0	33.131935	3
TCGCACC	1130	0.0	32.400406	1
GTACATG	5085	0.0	32.166634	1
TCAACGC	2280	0.0	31.242832	4
CGCACCA	1205	0.0	31.13394	2
TACATGG	5395	0.0	30.192331	2
ACATGGG	5505	0.0	28.985176	3
AATGCGG	295	0.0	27.366604	7
CCCATAA	1150	0.0	26.84167	2
CATAATA	1130	0.0	26.476225	4
CATGGGG	4165	0.0	26.338495	4
AATGCCG	235	5.456968E-12	26.281101	9
GCCCATA	1195	0.0	26.261177	1
CCATAAT	1140	0.0	26.243979	3
ACGCAGA	2780	0.0	26.13609	7
CTGTGCG	1510	0.0	26.113747	9
CGCAGAG	2845	0.0	25.707165	8
GACCGCA	1055	0.0	25.657621	7
>>END_MODULE