##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	998234
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16333244509804	34.0	33.0	34.0	31.0	34.0
2	33.287615929731906	34.0	33.0	34.0	31.0	34.0
3	33.4035186138721	34.0	34.0	34.0	31.0	34.0
4	36.62222384731436	37.0	37.0	37.0	35.0	37.0
5	36.65360626867047	37.0	37.0	37.0	35.0	37.0
6	36.69801269041127	37.0	37.0	37.0	36.0	37.0
7	36.67924654940625	37.0	37.0	37.0	36.0	37.0
8	36.668009705139276	37.0	37.0	37.0	35.0	37.0
9	38.51318829052106	39.0	39.0	39.0	37.0	39.0
10-11	38.536956264763575	39.0	39.0	39.0	37.5	39.0
12-13	38.514161008340736	39.0	39.0	39.0	37.0	39.0
14-15	40.11140774607958	41.0	40.0	41.0	38.0	41.0
16-17	40.12170493090798	41.0	40.0	41.0	38.0	41.0
18-19	40.108745043747255	41.0	40.0	41.0	38.0	41.0
20-21	40.104602728418385	41.0	40.0	41.0	38.0	41.0
22-23	40.03228201002971	41.0	40.0	41.0	38.0	41.0
24-25	39.990089998938124	41.0	40.0	41.0	38.0	41.0
26-27	39.916685867241554	41.0	40.0	41.0	38.0	41.0
28-29	39.89261485783894	41.0	40.0	41.0	38.0	41.0
30-31	39.83974348699804	41.0	40.0	41.0	38.0	41.0
32-33	39.80320546084385	41.0	40.0	41.0	37.0	41.0
34-35	39.72626859033052	41.0	40.0	41.0	37.0	41.0
36-37	39.60107800375463	41.0	40.0	41.0	37.0	41.0
38-39	39.47746971151053	41.0	39.5	41.0	36.0	41.0
40-41	39.33104612746109	41.0	39.0	41.0	35.5	41.0
42-43	39.21755620425672	41.0	39.0	41.0	35.0	41.0
44-45	38.95011540380312	40.5	38.5	41.0	35.0	41.0
46-47	38.70707118771751	40.0	38.0	41.0	35.0	41.0
48-49	38.57561703969209	40.0	37.5	41.0	35.0	41.0
50-51	38.3757686073606	40.0	37.0	41.0	35.0	41.0
52-53	38.08152296956425	40.0	36.0	41.0	35.0	41.0
54-55	37.84214723201173	39.0	35.5	41.0	34.0	41.0
56-57	37.56939805696861	39.0	35.0	41.0	34.0	41.0
58-59	37.26900005409553	38.5	35.0	41.0	34.0	41.0
60-61	36.98836094542963	37.5	35.0	41.0	33.0	41.0
62-63	36.76629026861437	37.0	35.0	40.5	33.0	41.0
64-65	36.505446618728676	36.5	35.0	40.0	33.0	41.0
66-67	36.23891742817816	36.0	35.0	40.0	33.0	41.0
68-69	35.98547835477453	35.5	35.0	39.0	33.0	41.0
70-71	35.71849736634897	35.0	35.0	39.0	33.0	41.0
72-73	35.448423916636784	35.0	35.0	38.5	33.0	41.0
74-75	35.172327830949456	35.0	35.0	37.0	32.0	40.0
76-77	33.39042949849434	34.5	32.0	35.5	29.0	38.5
78-79	34.70724449377601	35.0	34.5	36.0	32.0	39.0
80-81	34.65726222508951	35.0	35.0	36.0	33.0	38.5
82-83	34.48218153258655	35.0	35.0	36.0	33.0	37.0
84-85	34.3100956288806	35.0	35.0	35.5	32.5	37.0
86-87	34.150700637325514	35.0	35.0	35.0	32.0	36.5
88-89	34.02948907771124	35.0	35.0	35.0	32.0	36.0
90-91	33.8919156229902	35.0	34.5	35.0	32.0	36.0
92-93	33.813626865043666	35.0	34.0	35.0	32.0	36.0
94-95	33.72354628273531	35.0	34.0	35.0	31.5	35.5
96-97	33.69062464311975	35.0	34.0	35.0	31.0	35.0
98-99	33.66892381946518	35.0	34.0	35.0	31.0	35.0
100-101	32.81375459060701	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	13.0
15	34.0
16	88.0
17	194.0
18	372.0
19	538.0
20	793.0
21	1121.0
22	1649.0
23	1869.0
24	1924.0
25	2531.0
26	3382.0
27	4834.0
28	6467.0
29	7515.0
30	7775.0
31	8492.0
32	10184.0
33	13740.0
34	25231.0
35	54065.0
36	159212.0
37	306162.0
38	288874.0
39	91166.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71738561565249	18.451559008836732	13.004891402806015	23.826163972704766
2	16.57046343843227	19.079193856350315	38.44489368224284	25.905449022974576
3	20.405135469238676	26.050405015256946	27.704926900907	25.839532614597378
4	13.264024266855268	16.009372551926702	37.82109204855775	32.90551113266027
5	13.920383397079242	38.278299476876164	34.12135831879098	13.679958807253609
6	31.199097606372856	35.389497853208766	19.025098323639547	14.386306216778832
7	28.340549410258514	30.222372710206223	21.658548997529635	19.778528882005624
8	27.01851469695482	33.56427450878251	19.616843345347885	19.800367448914784
9	27.306823850920726	15.08443911948501	19.274639012496067	38.33409801709819
10-11	27.52556013920584	24.03053792998435	26.605936083122796	21.837965847687016
12-13	27.529817657984	22.161086478721423	26.886080818725873	23.42301504456871
14-15	24.409657455065645	24.316943722614138	24.16197003908903	27.111428783231183
16-17	24.1259564390714	26.942931216528386	25.419040024683593	23.51207231971662
18-19	24.167129150079038	26.105903024741693	26.278908552503722	23.448059272675543
20-21	25.175962156866284	24.669513717334553	26.02151216257641	24.13301196322276
22-23	25.589071780554047	25.145583702238877	25.16126156179531	24.10408295541177
24-25	25.203510213077145	24.868867896176234	25.3165603117518	24.61106157899482
26-27	24.5326161872781	26.406859837950904	25.557995463907613	23.502528510863378
28-29	24.167750286015398	26.273294476646917	26.2153901330197	23.34356510431798
30-31	24.28772427900882	25.092701906831767	26.140209661835268	24.479364152324145
32-33	24.311496258140007	25.500446538810813	26.418618489561812	23.769438713487375
34-35	25.18032458425165	24.863404127429373	25.446553796834305	24.509717491484672
36-37	23.885387646173125	25.072153649957873	26.263427691574076	24.779031012294922
38-39	24.27436853483251	25.426302850834574	25.390088896992086	24.90923971734082
40-41	24.528016477098554	25.342154244395605	25.997711959320156	24.13211731918568
42-43	24.58656988241234	25.45024513290471	26.031471578808173	23.931713405874774
44-45	24.811275293118147	24.83617142565173	25.79800370085769	24.55454958037243
46-47	24.536448258005912	24.666983145259163	25.835330540501655	24.961238056233267
48-49	23.983398582287812	24.854241716321322	26.3048121270892	24.857547574301663
50-51	24.576301748888536	25.02399237052635	25.939358907831227	24.460346972753882
52-53	24.53989973287813	25.413492935270156	25.233725375876485	24.81288195597523
54-55	24.793886002680736	24.580208648473203	25.625955437302277	24.999949911543787
56-57	25.04070001011865	24.86377393536287	25.387990065691067	24.70753598882741
58-59	25.018276895849002	25.049494184715154	25.758773035277542	24.173455884158304
60-61	24.518242429738628	25.713310843547347	25.312282519544116	24.45616420716991
62-63	24.10757397564098	25.011670610297788	26.06833668258144	24.812418731479795
64-65	24.64296948410894	24.58521749409457	25.526529851718134	25.24528317007836
66-67	24.18651338263373	25.03866828819696	25.230657340863967	25.544160988305347
68-69	24.67682928050938	24.98527399387318	26.092629583845074	24.24526714177237
70-71	25.05294349821785	24.88449601997127	25.786438851010885	24.27612163079999
72-73	24.259943059442975	25.18998551441846	26.041238827769842	24.50883259836872
74-75	24.573396618428145	24.661502212907997	25.997711959320156	24.7673892093437
76-77	24.58183526658659	23.993694846182898	25.792827511868495	25.63164237536202
78-79	24.69570305005753	23.975629194273672	25.670745665906768	25.657922089762025
80-81	23.995736246364014	25.220663777078617	25.606815994886702	25.17678398167067
82-83	23.708394253997973	25.197938940589015	26.207331116397164	24.886335689015848
84-85	24.312884968997114	24.266452016527527	26.391999306761534	25.028663707713832
86-87	23.964270902413663	25.676845308815366	25.751076400924035	24.60780738784694
88-89	24.6040006651747	24.381407565761133	25.063962958584863	25.950628810479305
90-91	24.429141864532763	24.56818741898192	25.21748407687977	25.785186639605545
92-93	24.422003680503447	24.828171378826386	25.90201886733859	24.847806073331576
94-95	24.392176583847075	24.331068667266393	25.80837759483248	25.46837715405406
96-97	24.81847943468165	24.879086466700194	25.759691615392782	24.54274248322538
98-99	22.95503859816436	25.348164859141242	26.311265695217756	25.385530847476645
100-101	23.929149459693953	24.86279950658447	25.53094548420033	25.677105549521244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	3.5
3	6.0
4	4.5
5	3.0
6	4.5
7	6.0
8	15.0
9	21.5
10	21.0
11	22.5
12	25.5
13	29.0
14	31.5
15	32.5
16	33.5
17	41.0
18	53.5
19	60.0
20	56.0
21	73.0
22	104.5
23	124.5
24	159.0
25	217.5
26	309.5
27	410.5
28	504.5
29	663.5
30	984.5
31	1728.0
32	3219.0
33	5594.0
34	8946.5
35	13767.5
36	20066.0
37	26133.5
38	29387.5
39	30181.5
40	29890.5
41	28695.0
42	27649.5
43	27192.0
44	27289.5
45	28408.5
46	30217.0
47	32173.0
48	34225.0
49	39083.0
50	45581.0
51	46323.5
52	44704.0
53	47263.0
54	52383.5
55	53068.5
56	44597.5
57	37848.5
58	36625.0
59	35689.5
60	29998.0
61	22015.5
62	17890.0
63	12448.0
64	6999.5
65	4442.0
66	3380.5
67	2894.0
68	2112.0
69	1222.5
70	694.0
71	507.5
72	458.5
73	427.0
74	315.5
75	207.0
76	121.0
77	65.0
78	39.0
79	16.5
80	8.0
81	7.0
82	7.5
83	4.5
84	1.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4662233504368715
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.014152994187736E-4
22-23	0.0011019460367008137
24-25	4.007076497093868E-4
26-27	0.0018031844236922406
28-29	0.003606368847384481
30-31	7.513268432051002E-4
32-33	5.008845621367335E-5
34-35	0.0034060150225297876
36-37	0.0017030075112648938
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008715391381179162
46-47	0.08009144148566368
48-49	6.010614745640802E-4
50-51	0.0
52-53	5.008845621367335E-5
54-55	0.0
56-57	0.007713622256905695
58-59	0.03901890739045154
60-61	0.030002985271990336
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.005307372820401E-4
78-79	0.007262826150982635
80-81	0.005259287902435701
82-83	9.516806680597936E-4
84-85	0.0020536267047606073
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.001769124273467E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06987339641807432
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	998234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.70027704531871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.07897441798025	26.346751709370004
2	16.11222588830431	12.470952113882658
3	6.369391574775168	7.394916556617534
4	3.059721826058395	4.736483294002734
5	1.7901129614697004	3.4638933775646668
6	1.0921700687243503	2.5360370544142277
7	0.678010933901633	1.8367447687223926
8	0.4868832995477207	1.5074014864988547
9	0.35352456100387397	1.2313348607857137
>10	1.6485001736245821	11.413144982293188
>50	0.14682522952070415	4.02128443174189
>100	0.15590268096519805	13.187614507045476
>500	0.02023362581015856	5.274423380190818
>1k	0.007522758314033311	4.579017476869822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3345	0.3350917720694747	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	2513	0.25174458092992225	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2449	0.24533325853457205	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	2395	0.23992370526349535	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2213	0.22169150720171824	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1834	0.18372445739175383	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1731	0.17340623541173714	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1673	0.16759597449095104	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1655	0.16579279006725878	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1620	0.16228659813230165	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1615	0.1617857135701649	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1615	0.1617857135701649	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1535	0.15377156057597718	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1522	0.15246926071442166	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1482	0.1484621842173278	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1429	0.14315280785867843	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1400	0.14024767739828536	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1318	0.13203317057924294	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1316	0.13183281675438827	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1296	0.1298292785058413	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1157	0.11590468767844013	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1147	0.11490291855416666	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	1093	0.10949336528308994	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	1077	0.10789053468425239	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	1060	0.1061875271729875	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	1059	0.10608735026056014	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1035	0.10368310436230384	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1016	0.10177974302618424	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1012	0.10137903537647484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.0017691242734669E-4	0.0
22-23	0.0	0.0	0.0	3.005307372820401E-4	0.0
24-25	0.0	0.0	0.0	7.513268432051002E-4	0.0
26-27	0.0	0.0	0.0	0.0016028305988375472	0.0
28-29	0.0	0.0	0.0	0.003606368847384481	0.0
30-31	0.0	0.0	0.0	0.009416629768170589	0.0
32-33	0.0	0.0	0.0	0.020335913222751377	0.0
34-35	0.0	0.0	0.0	0.03380970794422951	0.0
36-37	0.0	0.0	0.0	0.06075729738718577	0.0
38-39	0.0	0.0	0.0	0.10668841173512422	0.0
40-41	0.0	0.0	0.0	0.16639385154182285	0.0
42-43	0.0	0.0	0.0	0.21888655365375254	0.0
44-45	0.0	0.0	0.0	0.2776403127923913	0.0
46-47	0.0	0.0	0.0	0.34981777819629467	0.0
48-49	0.0	0.0	0.0	0.4295586004884626	0.0
50-51	0.0	0.0	0.0	0.52197180220269	0.0
52-53	0.0	0.0	0.0	0.6099271313139003	0.0
54-55	0.0	0.0	0.0	0.6958288337203501	0.0
56-57	0.0	0.0	0.0	0.7928000849500217	0.0
58-59	0.0	0.0	0.0	0.9071019420296244	0.0
60-61	0.0	0.0	0.0	1.0380331665721665	0.0
62-63	0.0	0.0	0.0	1.1786314631639474	0.0
64-65	0.0	0.0	0.0	1.310263926093481	0.0
66-67	0.0	0.0	0.0	1.4618816830522703	0.0
68-69	0.0	0.0	0.0	1.6354381838326484	0.0
70-71	0.0	0.0	0.0	1.8315845783653932	0.0
72-73	0.0	0.0	0.0	2.043859455798941	0.0
74-75	0.0	0.0	0.0	2.2483205340631556	0.0
76-77	0.0	0.0	0.0	2.4656042571180707	0.0
78-79	0.0	0.0	0.0	2.7145939729562407	0.0
80-81	0.0	0.0	0.0	2.990881897430863	0.0
82-83	0.0	0.0	0.0	3.290511042501057	0.0
84-85	0.0	0.0	0.0	3.591943371994943	0.0
86-87	0.0	0.0	0.0	3.9084022383529313	0.0
88-89	0.0	0.0	0.0	4.264080365926226	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	770	0.0	57.652454	1
GCGTAAT	50	2.7458813E-5	47.733753	1
AAACCGT	190	0.0	47.49226	6
GTATCAA	1605	0.0	46.990234	1
ACCGTGC	280	0.0	37.315346	8
CCGTGCG	230	0.0	37.16786	9
AACCGTG	300	0.0	34.82766	7
ATCAACG	2160	0.0	34.739708	3
GTCGCTA	55	0.0025815256	34.53983	9
TCGCACC	1130	0.0	32.10412	1
TCAACGC	2365	0.0	31.728447	4
ATCACGC	120	1.507633E-7	31.661505	3
GTATCAC	245	0.0	31.173063	1
GTACATG	5305	0.0	31.132664	1
CGCACCA	1165	0.0	30.982075	2
ACATGGG	5435	0.0	29.44782	3
TACATGG	5580	0.0	29.278385	2
CGCAGAG	2770	0.0	27.603804	8
GCTATAG	540	0.0	27.402708	1
CATGGGG	4090	0.0	27.171614	4
>>END_MODULE