##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224556_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1029075
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33569856424459	34.0	31.0	34.0	31.0	34.0
2	32.49454995991546	34.0	31.0	34.0	31.0	34.0
3	32.63197337414668	34.0	31.0	34.0	31.0	34.0
4	35.903781551393244	37.0	37.0	37.0	35.0	37.0
5	35.9260432913053	37.0	37.0	37.0	35.0	37.0
6	36.02143186842553	37.0	37.0	37.0	35.0	37.0
7	35.98504190656658	37.0	37.0	37.0	35.0	37.0
8	36.0242373004883	37.0	37.0	37.0	35.0	37.0
9	37.77427981439642	39.0	39.0	39.0	35.0	39.0
10-11	37.84710055146612	39.0	39.0	39.0	37.0	39.0
12-13	37.83895440079683	39.0	39.0	39.0	37.0	39.0
14-15	39.342699511697404	41.0	40.0	41.0	37.0	41.0
16-17	39.18854505259578	41.0	40.0	41.0	36.5	41.0
18-19	39.27426620994582	41.0	40.0	41.0	36.5	41.0
20-21	39.33196025556932	41.0	40.0	41.0	37.0	41.0
22-23	39.29292811505478	41.0	40.0	41.0	37.0	41.0
24-25	39.228709763622675	41.0	40.0	41.0	36.0	41.0
26-27	39.06388212715302	41.0	39.5	41.0	35.5	41.0
28-29	39.00337050263586	41.0	39.0	41.0	35.0	41.0
30-31	38.93028933751184	41.0	39.0	41.0	35.0	41.0
32-33	38.750283021159774	41.0	39.0	41.0	35.0	41.0
34-35	38.75356752423293	41.0	39.0	41.0	35.0	41.0
36-37	38.73013337220319	41.0	39.0	41.0	35.0	41.0
38-39	38.60779243495372	41.0	39.0	41.0	35.0	41.0
40-41	38.51638218788718	40.5	39.0	41.0	35.0	41.0
42-43	38.37235672812963	40.0	38.0	41.0	34.5	41.0
44-45	38.184951534144744	40.0	38.0	41.0	34.0	41.0
46-47	38.01843791754732	40.0	38.0	41.0	33.5	41.0
48-49	37.79588951242621	40.0	37.0	41.0	33.0	41.0
50-51	36.481087870174676	38.5	35.5	40.0	31.5	40.5
52-53	36.76195758326652	39.0	35.5	40.0	31.5	40.5
54-55	37.182563467191414	39.0	35.5	41.0	33.0	41.0
56-57	37.01173918324709	39.0	35.0	41.0	32.5	41.0
58-59	36.85551976289386	39.0	35.0	41.0	32.5	41.0
60-61	36.611711974345894	38.5	35.0	41.0	32.0	41.0
62-63	36.35082428394432	38.0	35.0	41.0	31.5	41.0
64-65	36.121356558074	37.0	35.0	41.0	31.0	41.0
66-67	35.919056434176326	37.0	35.0	40.5	31.0	41.0
68-69	35.654794354153	36.0	35.0	40.0	31.0	41.0
70-71	35.40841921142774	36.0	35.0	39.5	30.5	41.0
72-73	35.11762505162403	35.0	35.0	39.0	30.0	41.0
74-75	34.86139348443991	35.0	35.0	39.0	30.0	41.0
76-77	34.5557981682579	35.0	34.5	38.0	29.5	40.0
78-79	34.318198867915356	35.0	34.0	37.0	30.0	39.0
80-81	34.007594198673566	35.0	34.0	37.0	29.0	39.0
82-83	33.72037169302529	35.0	34.0	36.0	29.0	38.5
84-85	33.46699220173457	35.0	34.0	36.0	29.0	37.0
86-87	33.22651944707626	35.0	34.0	36.0	29.0	37.0
88-89	33.06495153414474	35.0	34.0	35.0	28.5	36.5
90-91	32.87316813643321	35.0	34.0	35.0	27.5	36.0
92-93	32.718132303282076	35.0	34.0	35.0	27.0	36.0
94-95	32.581030051259624	35.0	33.0	35.0	27.0	36.0
96-97	32.43504409299614	35.0	33.0	35.0	26.5	35.5
98-99	32.390892792070545	35.0	33.0	35.0	26.0	35.0
100-101	31.177633311469037	34.5	31.0	35.0	22.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	294.0
8	1227.0
9	946.0
10	746.0
11	983.0
12	1525.0
13	1710.0
14	1739.0
15	1683.0
16	1847.0
17	2023.0
18	2359.0
19	2529.0
20	3095.0
21	4189.0
22	5242.0
23	5353.0
24	5254.0
25	5454.0
26	5639.0
27	5659.0
28	6013.0
29	6849.0
30	8506.0
31	10997.0
32	14732.0
33	21252.0
34	36917.0
35	67734.0
36	147960.0
37	247834.0
38	283428.0
39	117326.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66214949769406	20.619116529335	14.274481028376904	23.444252944594044
2	15.51443336436447	21.282045800399192	38.437566200292686	24.76595463494365
3	19.055906680970818	28.434939440583364	28.93811123031236	23.571042648133464
4	12.912469936593544	18.14435293831839	40.11806719626849	28.825109928819572
5	13.601341010130458	39.54055826834779	33.82037266477176	13.037728056749994
6	26.968296771372348	38.662779680781284	20.66273109345772	13.706192454388649
7	25.594441610183903	31.92080266258533	23.98377183392853	18.500983893302237
8	25.48988168986711	34.142506620022836	21.925709982265627	18.441901707844423
9	27.1433083108617	15.801083497315553	21.019847921677233	36.035760270145516
10-11	25.669314675801086	25.57179991740155	28.05198843621699	20.706896970580377
12-13	26.088866214804558	23.40320190462308	28.076767971236304	22.431163909336053
14-15	23.530709780460075	25.068786972542078	25.47249274462086	25.928010502376985
16-17	22.98950054408128	27.932617551216087	26.426499432580115	22.65138247212252
18-19	23.018503155786046	27.34475121850228	27.016075142067475	22.620670483644197
20-21	24.539872827683553	25.574496710842894	26.388790327049243	23.496840134424307
22-23	24.750755779072335	26.403405648919836	25.907591240450056	22.938247331557776
24-25	23.59166821767056	26.56055694241491	26.16605930488136	23.681715535033167
26-27	23.283677088647572	27.438719238150767	26.765930568714623	22.51167310448704
28-29	23.00507737531278	27.18684255277798	27.090688239438332	22.71739183247091
30-31	23.207103466705536	26.475864247017956	26.94332288705877	23.37370939921774
32-33	23.18198382042125	26.482277773728836	27.37380657386488	22.961931831985037
34-35	23.744673614653937	26.166460170541505	26.840755047008237	23.248111167796324
36-37	22.82728339655009	26.315196077240266	27.65278327870207	23.204737247507577
38-39	23.008930827389165	26.626693358729664	26.66114361236905	23.70323220151212
40-41	23.633476527063713	26.259650514005727	27.18154214837242	22.925330810558144
42-43	23.387218246043663	26.63547269555753	27.155275036563026	22.82203402183578
44-45	23.462206042135175	26.133799299107658	26.699071113555835	23.704923545201336
46-47	23.53215266137065	26.04732405315453	26.48810825255691	23.932415032917913
48-49	22.834438694944488	26.311250394772006	26.929524087165657	23.92478682311785
50-51	22.95396351092	26.746787163229115	26.587712265869833	23.71153705998105
52-53	23.34159317833977	26.578043388479944	26.01005757597843	24.070305857201856
54-55	23.256613949420597	25.96482277773729	26.66909603284503	24.109467239997084
56-57	23.19419050807303	26.564306082795753	26.495263462460688	23.746239946670528
58-59	23.72232907613106	26.463301918608096	26.545815381714664	23.268553623546172
60-61	23.357271566277706	26.63364104119164	26.568765805745564	23.440321586785085
62-63	22.804543179156198	26.287695799454703	27.2394889166452	23.6682721047439
64-65	23.98671787540449	25.83019354939065	26.23418907535044	23.948899499854413
66-67	23.216713755745207	26.23124090655952	26.136528198534165	24.415517139161107
68-69	23.077812598693	26.26110827685057	27.447076257804337	23.214002866652088
70-71	23.8314505745451	25.94917765954862	26.78279036999247	23.436581395913805
72-73	23.30019677866045	26.20392099701188	27.137234895415784	23.358647328911886
74-75	23.431819838204213	25.805845055025145	27.02140271603139	23.740932390739257
76-77	23.199280907611204	25.66848869130044	26.26737604159075	24.864854359497606
78-79	23.366515617217207	25.2501548965534	27.00764156330106	24.375687922928336
80-81	23.088063284456283	26.36171741537911	26.529826475280966	24.02039282488364
82-83	22.87725691085524	26.87346326865734	26.676975547438513	23.572304273048907
84-85	23.092512841648105	25.731654389197317	27.328786530473188	23.84704623868139
86-87	23.070292753999425	26.757197312763402	26.62024917075153	23.55226076248564
88-89	23.304085708356155	26.011414884842644	26.34264387318717	24.34185553361403
90-91	23.09996841823968	25.87255545028302	26.558025411170227	24.469450720307073
92-93	23.179209849909384	25.839297601173904	26.97510798847475	24.006384560441962
94-95	23.475111143502662	25.448582464834924	26.935840439229402	24.14046595243301
96-97	23.54162718946627	26.32237689186891	26.724048295799623	23.411947622865195
98-99	22.520377662117568	26.55597103398415	26.943992039398758	23.979659264499528
100-101	23.43042967867064	25.77999110456511	26.376131069129194	24.41344814763505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	3.5
3	4.0
4	2.5
5	2.0
6	3.0
7	7.0
8	14.5
9	20.5
10	22.5
11	30.5
12	34.0
13	39.0
14	41.0
15	43.0
16	46.0
17	55.5
18	68.5
19	70.0
20	89.5
21	105.0
22	113.5
23	148.0
24	199.5
25	246.5
26	312.0
27	443.5
28	588.0
29	779.0
30	1230.5
31	2415.0
32	4808.0
33	8846.5
34	15185.5
35	24472.0
36	37165.0
37	48518.0
38	53223.5
39	53059.5
40	49183.5
41	42300.5
42	35833.5
43	30560.5
44	26779.0
45	26146.0
46	27670.5
47	27345.0
48	28429.0
49	33467.0
50	38590.5
51	39095.5
52	38005.5
53	39502.5
54	42438.5
55	43416.5
56	36304.0
57	29828.5
58	28493.5
59	27509.5
60	23122.0
61	17328.5
62	13854.5
63	9749.0
64	5917.5
65	3866.0
66	3070.0
67	2705.0
68	2149.0
69	1367.0
70	773.5
71	458.0
72	337.0
73	262.5
74	211.5
75	166.5
76	108.0
77	71.0
78	45.5
79	28.5
80	19.0
81	13.5
82	12.5
83	9.5
84	7.5
85	6.5
86	8.0
87	7.5
88	5.0
89	3.0
90	3.0
91	4.5
92	3.0
93	3.5
94	5.0
95	2.5
96	3.0
97	4.5
98	3.5
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14712241576172777
2	9.71746471345626E-5
3	2.9152394140368777E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.034545587056337
16-17	0.07152054029103806
18-19	0.02434224910720793
20-21	0.03809246167674854
22-23	0.06471831499161869
24-25	0.016665451983577485
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.020163739280421738
38-39	0.005344605592400942
40-41	0.004810145033160848
42-43	0.0029152394140368777
44-45	0.009814639360590823
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.85873235672813E-5
58-59	0.014770546364453513
60-61	0.017199912542817577
62-63	0.027208901197677524
64-65	0.04610937006534995
66-67	0.01690838860141389
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01579088015936642
80-81	0.05597259674950805
82-83	0.04975341933289605
84-85	0.028520758933994122
86-87	0.05670140660301728
88-89	0.03211622087797293
90-91	0.0
92-93	0.0029152394140368777
94-95	0.0
96-97	0.0
98-99	6.802225299419382E-4
100-101	0.04445740106406238
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1029075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.406433325216184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.97106658030808	24.745841034998158
2	15.092141804258679	10.989021086629029
3	6.245612213282467	6.8214139385407195
4	3.1293184082408754	4.557092879319723
5	1.8231869450424856	3.318786697704691
6	1.1926884842782723	2.605292026837804
7	0.8214705796222571	2.093476971995108
8	0.5885541412786535	1.714172568219293
9	0.43284462827079806	1.4182496189386913
>10	2.309667187657416	15.218496976708037
>50	0.19199327116592618	4.942263202764755
>100	0.17495553626797852	13.273875520127529
>500	0.01973996003887548	4.776441171108621
>1k	0.006760260287286123	3.5255763061078755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2665	0.2589704346136093	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2174	0.2112576828705391	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	2060	0.20017977309719895	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1895	0.1841459563199961	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1890	0.1836600830843233	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1578	0.15334159317833976	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1560	0.15159244952991766	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1532	0.1488715594101499	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1491	0.14488739887763283	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1475	0.14333260452347982	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1361	0.1322546947501397	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1317	0.12797901027621894	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	1242	0.12069091174112676	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1232	0.11971916526978112	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1214	0.11797002162135899	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1171	0.11379151179457281	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1153	0.11204236814615068	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1144	0.11116779632193961	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1133	0.11009887520345941	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1122	0.10902995408497923	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1107	0.1075723343779608	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1090	0.10592036537667322	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1089	0.10582319072953866	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1074	0.10436557102252023	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1043	0.10135315696134879	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.717464713456259E-5	0.0
10-11	0.0	0.0	0.0	9.717464713456259E-5	0.0
12-13	0.0	0.0	0.0	1.9434929426912517E-4	0.0
14-15	0.0	0.0	0.0	1.9434929426912517E-4	0.0
16-17	0.0	0.0	0.0	2.4293661783640646E-4	0.0
18-19	0.0	0.0	0.0	4.3728591210553166E-4	0.0
20-21	0.0	0.0	0.0	6.316352063746568E-4	0.0
22-23	0.0	0.0	0.0	0.0012632704127493138	0.0
24-25	0.0	0.0	0.0	0.00223501688409494	0.0
26-27	0.0	0.0	0.0	0.003546874620411535	0.0
28-29	0.0	0.0	0.0	0.0054417802395355045	0.0
30-31	0.0	0.0	0.0	0.01423608580521342	0.0
32-33	0.0	0.0	0.0	0.03386536452639506	0.0
34-35	0.0	0.0	0.0	0.058401962927872114	0.0
36-37	0.0	0.0	0.0	0.10203337949129072	0.0
38-39	0.0	0.0	0.0	0.1816194154944975	0.0
40-41	0.0	0.0	0.0	0.2784539513640891	0.0
42-43	0.0	0.0	0.0	0.3763574083521609	0.0
44-45	0.0	0.0	0.0	0.4854359497607075	0.0
46-47	0.0	0.0	0.0	0.6095765614751112	0.0
48-49	0.0	0.0	0.0	0.751014260379467	0.0
50-51	0.0	0.0	0.0	0.9020722493501445	0.0
52-53	0.0	0.0	0.0	1.0448703933143844	0.0
54-55	0.0	0.0	0.0	1.1790685810072152	0.0
56-57	0.0	0.0	0.0	1.3360056361295338	0.0
58-59	0.0	0.0	0.0	1.5207346403323374	0.0
60-61	0.0	0.0	0.0	1.7192624444282487	0.0
62-63	0.0	0.0	0.0	1.92172582173311	0.0
64-65	0.0	0.0	0.0	2.11651240191434	0.0
66-67	0.0	0.0	0.0	2.3382649466754124	0.0
68-69	0.0	0.0	0.0	2.5941257925807157	0.0
70-71	0.0	0.0	0.0	2.8753492213881398	0.0
72-73	0.0	0.0	0.0	3.178145421859437	0.0
74-75	0.0	0.0	0.0	3.463887471758618	0.0
76-77	0.0	0.0	0.0	3.7632825595802055	0.0
78-79	0.0	0.0	0.0	4.101887617520589	0.0
80-81	0.0	0.0	0.0	4.497048320093287	0.0
82-83	0.0	0.0	0.0	4.909117411267401	0.0
84-85	0.0	0.0	0.0	5.305395622282147	0.0
86-87	0.0	0.0	0.0	5.729320020406676	0.0
88-89	0.0	0.0	0.0	6.18370867040789	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	930	0.0	57.2734	1
GTATCAA	2075	0.0	48.35955	1
ATCAACG	2725	0.0	35.72148	3
CCGTGCG	190	0.0	35.008205	9
TCAACGC	2835	0.0	34.33546	4
GCGTAAT	60	0.0039274814	31.704918	1
CAGTGCG	90	2.4088564E-5	31.67409	9
AATGCGG	420	0.0	30.52506	7
TATCAAC	3295	0.0	30.118473	2
AACCGTG	240	0.0	29.677145	7
CGCAGAG	3355	0.0	29.298206	8
GCCCATA	1160	0.0	29.108395	1
AAACCGT	245	0.0	29.071487	6
ATGCGGG	430	0.0	28.712307	8
CCCATAA	1195	0.0	28.609264	2
ACGCAGA	3460	0.0	28.407717	7
ACCGTGC	245	0.0	27.134708	8
GTACATG	5810	0.0	26.930079	1
ACATGGG	5760	0.0	26.297249	3
AAATGCG	490	0.0	26.16434	6
>>END_MODULE