FastQCFastQC Report
Fri 10 Feb 2017
SRR3224555_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224555_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874015
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC20710.23695245504939846No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA16700.1910722356023638No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA16430.1879830437692717No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG16140.1846650229115061No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13050.14931093859945196No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT12260.14027219212484912No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10800.12356767332368439No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA10780.12333884429901087No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC8980.10274423207839684No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA8860.10137125793035588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT3550.054.8415156
CCGTGCG3650.053.3664869
ACCGTGC3800.052.4891248
GGTATCA9800.051.9168471
AACCGTG3800.051.233527
GTATCAA16550.037.9250831
TGCGCGG3500.030.54511612-13
ATCAACG21050.029.7766463
AATGCGG2600.029.221377
TCAACGC21900.028.620934
ATGCGGG3000.026.911098
CGGCTTA3650.026.68629616-17
AAATGCG2900.026.198476
GTACATG33900.025.9491211
GTCTAAT7400.025.702711
GTGCGCG4200.025.45353510-11
TACATGG34300.025.1959782
CGCAGAG25450.024.818048
ACGCAGA25750.024.526097
ACATGGG34400.024.1564733
Produced by FastQC (version 0.11.5)