##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224555_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874015
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.078773247598726	34.0	31.0	34.0	30.0	34.0
2	32.243186901826626	34.0	31.0	34.0	30.0	34.0
3	32.37587341178355	34.0	31.0	34.0	31.0	34.0
4	35.68957283341819	37.0	35.0	37.0	35.0	37.0
5	35.69746056989869	37.0	37.0	37.0	35.0	37.0
6	35.795772383769155	37.0	37.0	37.0	35.0	37.0
7	35.753110644554155	37.0	37.0	37.0	35.0	37.0
8	35.788860603078895	37.0	37.0	37.0	35.0	37.0
9	37.507198389043666	39.0	38.0	39.0	35.0	39.0
10-11	37.57137291694079	39.0	38.5	39.0	35.0	39.0
12-13	37.568287729615626	39.0	38.0	39.0	35.0	39.0
14-15	39.031717419037435	41.0	39.0	41.0	36.0	41.0
16-17	38.864509762418265	41.0	39.5	41.0	35.5	41.0
18-19	38.94575264726578	41.0	39.5	41.0	35.5	41.0
20-21	39.01707236145833	41.0	40.0	41.0	36.0	41.0
22-23	38.96379867622409	41.0	39.0	41.0	35.5	41.0
24-25	38.892570493641415	41.0	39.0	41.0	35.0	41.0
26-27	38.711014685102654	41.0	39.0	41.0	35.0	41.0
28-29	38.62186289708987	41.0	39.0	41.0	35.0	41.0
30-31	38.53529172840283	40.5	39.0	41.0	34.5	41.0
32-33	38.329333592672896	40.0	38.0	41.0	34.0	41.0
34-35	38.31343684032883	40.0	38.0	41.0	34.0	41.0
36-37	38.30218245682282	40.0	38.0	41.0	34.0	41.0
38-39	38.18971070290556	40.0	38.0	41.0	34.0	41.0
40-41	38.08777938593731	40.0	38.0	41.0	33.0	41.0
42-43	37.925048769185885	40.0	38.0	41.0	33.0	41.0
44-45	37.73337700153887	40.0	37.5	41.0	33.0	41.0
46-47	37.53339587993341	40.0	37.0	41.0	32.0	41.0
48-49	37.3074277901409	40.0	36.5	41.0	31.5	41.0
50-51	36.016480838429544	38.5	35.0	40.0	29.5	40.5
52-53	36.36242970658398	39.0	35.0	40.0	30.5	40.5
54-55	36.82344524979548	39.0	35.0	41.0	31.0	41.0
56-57	36.66173749878435	39.0	35.0	41.0	31.0	41.0
58-59	36.51393168309468	39.0	35.0	41.0	31.0	41.0
60-61	36.26045891660898	38.5	35.0	41.0	30.0	41.0
62-63	35.97294897684822	38.0	35.0	41.0	30.0	41.0
64-65	35.70784025445788	37.0	35.0	40.0	29.0	41.0
66-67	35.450278313301254	36.5	35.0	40.0	29.0	41.0
68-69	35.12661853629514	36.0	35.0	39.5	29.0	41.0
70-71	34.82200591523029	35.5	34.5	39.0	29.0	41.0
72-73	34.49420032836965	35.0	34.0	39.0	28.0	41.0
74-75	34.21007419781125	35.0	34.0	37.5	28.0	40.0
76-77	33.861234647002625	35.0	34.0	37.0	27.0	39.0
78-79	33.625480683969954	35.0	34.0	37.0	27.0	39.0
80-81	33.32884332648753	35.0	34.0	36.0	27.0	38.5
82-83	33.07338775650304	35.0	34.0	36.0	27.0	37.0
84-85	32.855769065748305	35.0	33.0	35.5	26.0	37.0
86-87	32.63552570608056	35.0	33.0	35.0	26.0	36.5
88-89	32.47921603176147	35.0	33.0	35.0	26.0	36.0
90-91	32.312158830226025	35.0	33.0	35.0	25.0	36.0
92-93	32.17576757835964	35.0	33.0	35.0	25.0	36.0
94-95	32.04413883056927	35.0	33.0	35.0	25.0	35.0
96-97	31.913663953135817	35.0	33.0	35.0	24.0	35.0
98-99	31.864383906454695	35.0	33.0	35.0	24.0	35.0
100-101	30.670938713866466	34.0	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	4.0
7	93.0
8	376.0
9	331.0
10	502.0
11	995.0
12	1795.0
13	2139.0
14	2137.0
15	2108.0
16	2320.0
17	2469.0
18	2884.0
19	3256.0
20	3991.0
21	5013.0
22	5968.0
23	5527.0
24	5357.0
25	5487.0
26	5716.0
27	6050.0
28	6809.0
29	7844.0
30	9455.0
31	11755.0
32	15343.0
33	21143.0
34	33765.0
35	59385.0
36	124016.0
37	217171.0
38	240232.0
39	62557.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93412809997376	19.387218419885667	13.77721554378378	23.901437936356793
2	15.91860551592364	19.860071051412163	38.95917118127264	25.26215225139157
3	18.689903571118016	26.509590257342	29.00040274046581	25.800103431074174
4	12.426560184893852	17.276248119311454	39.286396686555726	31.010795009238972
5	13.545877359084226	38.85951614102733	34.29609331647626	13.298513183412183
6	29.45830449134168	37.886992786164996	19.217061492079658	13.437641230413666
7	26.427578474053647	31.6747424243291	23.196627060176315	18.701052041440935
8	25.90344559303902	34.328930281516904	20.877788138647507	18.889835986796566
9	26.51933891294772	15.3527113379061	20.56486444740651	37.563085301739676
10-11	25.68754540825958	25.27468064049244	27.970458172914654	21.067315778333324
12-13	26.20429855322849	22.98118453344622	28.097515488864605	22.71700142446068
14-15	23.516168445992154	24.73192799526149	25.25825733882344	26.493646219922912
16-17	23.41730193660964	27.14533846587175	26.296568969820765	23.140790627697847
18-19	23.181076373391196	26.738755255863566	27.165862121846768	22.914306248898473
20-21	24.70584936122139	25.0494614892873	26.815538547912542	23.429150601578762
22-23	24.49433931373374	25.991242637558653	26.05776149725824	23.456656551449363
24-25	23.578161020163996	25.87658542362931	26.658232816602666	23.88702073960402
26-27	23.498452543720646	26.696910236094347	26.786554006510187	23.01808321367482
28-29	23.311442023306235	26.846736039999314	26.680606168086364	23.16121576860809
30-31	23.516072378620503	25.84835500534888	26.912066726543593	23.723505889487022
32-33	23.554973312814997	25.907907759020155	27.08557633450227	23.45154259366258
34-35	23.95559572776211	25.81317254280533	26.68106382613571	23.550167903296853
36-37	23.447046988852176	25.836086623644	27.22890628665729	23.487960100846532
38-39	23.364043804757376	26.09749316051256	26.626974183294834	23.911488851435234
40-41	24.050194775363277	25.625217759943155	26.986156617814068	23.338430846879504
42-43	23.8683132280605	25.96909779709674	26.80801571655284	23.35457325828992
44-45	23.938226589091357	25.78316713458637	26.410410302739372	23.868195973582896
46-47	23.885802875236696	25.624216975681197	26.19382962534968	24.29615052373243
48-49	23.416588960143706	26.017516861838757	26.51247404220751	24.05342013581003
50-51	23.577112520952156	26.244229218034015	26.178498080696556	24.00016018031727
52-53	24.020068305463866	26.13376200637289	25.723757601414164	24.122412086749083
54-55	23.820872639485593	25.585201626974364	26.213966579520946	24.379959154019097
56-57	23.79003792841084	26.19834899858698	25.95933708231552	24.05227599068666
58-59	24.23641475686915	26.077990916279404	26.162385523721465	23.52320880312998
60-61	23.97686114973308	26.29560968352873	25.978222931722083	23.749306235016107
62-63	23.835675544522612	25.885868563783337	26.43866016135106	23.839795730342995
64-65	24.484022143262695	25.498177622300776	25.81800948735325	24.19979074708328
66-67	23.63496128661636	25.884640296249508	25.798012949472337	24.682385467661796
68-69	23.807028483492847	26.114197124763304	26.425118562038406	23.65365582970544
70-71	24.209138287100334	25.56855431542937	26.233703082899034	23.988604314571262
72-73	24.071440421503063	25.664204847742887	26.50068934743683	23.76366538331722
74-75	24.01560613948273	25.628049861844477	26.27918285155289	24.077161147119902
76-77	23.857370868921013	25.460089357734134	25.782109002705905	24.90043077063895
78-79	23.95141536396422	25.081291951252865	26.323200662809054	24.644092021973858
80-81	23.767955832729918	25.942547059379105	25.999387519712418	24.290109588178556
82-83	23.702554739303263	26.154211585186115	26.229763414026884	23.913470261483734
84-85	23.717981731804766	25.484830485294	26.65082705871985	24.14636072418138
86-87	23.753822975690365	26.313635408754305	26.0264578824421	23.906083733113224
88-89	24.03395112772704	25.71937557118847	25.802010125594222	24.44466317549027
90-91	23.678083328089336	25.632626442337948	26.118716498000605	24.57057373157211
92-93	23.650392369209786	25.667130633174118	26.509364285791925	24.173112711824167
94-95	23.962117354965304	25.632740856850283	26.251265710542725	24.15387607764169
96-97	23.88843440902044	26.19611791559641	26.17620979044982	23.739237884933324
98-99	23.107991373047064	26.45070680373682	26.263236480340503	24.178065342875612
100-101	23.90443103902199	25.718627305090376	26.00262130699053	24.374320348897104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	3.5
3	4.0
4	3.0
5	3.5
6	9.0
7	14.0
8	23.0
9	36.5
10	40.5
11	46.0
12	61.0
13	71.5
14	79.5
15	96.5
16	107.5
17	118.0
18	130.0
19	147.0
20	198.0
21	249.0
22	316.0
23	428.5
24	559.0
25	813.5
26	1196.0
27	1750.0
28	2585.5
29	3661.5
30	5076.5
31	6803.0
32	8916.5
33	11098.0
34	13396.0
35	15656.0
36	18177.0
37	20743.5
38	22384.0
39	23514.5
40	24313.0
41	25084.5
42	25927.5
43	26701.5
44	28159.5
45	30934.0
46	33649.0
47	34395.5
48	35531.0
49	38139.0
50	40611.0
51	39581.5
52	37479.5
53	38436.5
54	38934.0
55	37412.0
56	31859.0
57	26699.0
58	24696.5
59	22771.5
60	19157.5
61	14668.5
62	11603.0
63	8422.0
64	5368.5
65	3588.5
66	2827.5
67	2383.0
68	1868.0
69	1245.5
70	796.5
71	558.0
72	419.5
73	329.5
74	265.5
75	205.0
76	136.0
77	81.5
78	53.5
79	37.0
80	26.0
81	27.5
82	21.5
83	13.0
84	11.5
85	8.0
86	7.0
87	7.5
88	5.5
89	5.5
90	5.5
91	3.5
92	2.0
93	2.5
94	3.5
95	4.0
96	5.5
97	5.5
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1494253531117887
2	0.0
3	3.4324353701023437E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0368986802286002
16-17	0.07242438630915946
18-19	0.026200923325114558
20-21	0.040674359135712775
22-23	0.06618879538680686
24-25	0.017791456668363816
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.024255876615389894
38-39	0.005263067567490261
40-41	0.006807663484036316
42-43	0.003661264394775833
44-45	0.012757218125547044
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.016475689776491248
60-61	0.018306321973879165
62-63	0.030663089306247605
64-65	0.049312654817137
66-67	0.01796307843686893
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01813470020537405
80-81	0.059209510134265425
82-83	0.05062842170900957
84-85	0.029290115158206665
86-87	0.058751852084918454
88-89	0.03300858680915087
90-91	0.0
92-93	0.0033752281139339713
94-95	0.0
96-97	0.0
98-99	0.0011441451233674478
100-101	0.04633787749638164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	874015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.228019836664494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29914762008325	45.59204320816128
2	13.553831430427534	15.784255307874822
3	4.0501596135878355	7.074983229649495
4	1.5869691604538887	3.6962428702033527
5	0.7949583364509556	2.314442489209402
6	0.4366447107563659	1.5254974127697782
7	0.25797276215072074	1.0514870178281908
8	0.16578055951534806	0.772245896639442
9	0.11779589476478786	0.6173119527337538
>10	0.5827929464129226	6.426656102281307
>50	0.07221394833315585	2.96360083813647
>100	0.07498805936599333	8.826609276243527
>500	0.005157908827383802	2.004619393532025
>1k	0.0015870488699642469	1.350005004737166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	2071	0.23695245504939846	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1670	0.1910722356023638	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1643	0.1879830437692717	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1614	0.1846650229115061	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1305	0.14931093859945196	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1226	0.14027219212484912	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1080	0.12356767332368439	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1078	0.12333884429901087	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	898	0.10274423207839684	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	886	0.10137125793035588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.144145123367448E-4	0.0
5	0.0	0.0	0.0	1.144145123367448E-4	0.0
6	0.0	0.0	0.0	1.144145123367448E-4	0.0
7	0.0	0.0	0.0	1.144145123367448E-4	0.0
8	0.0	0.0	0.0	1.144145123367448E-4	0.0
9	0.0	0.0	0.0	2.288290246734896E-4	0.0
10-11	0.0	0.0	0.0	2.288290246734896E-4	0.0
12-13	0.0	0.0	0.0	2.288290246734896E-4	0.0
14-15	0.0	0.0	0.0	2.288290246734896E-4	0.0
16-17	0.0	0.0	0.0	2.288290246734896E-4	0.0
18-19	0.0	0.0	0.0	3.4324353701023437E-4	0.0
20-21	0.0	0.0	0.0	4.576580493469792E-4	0.0
22-23	0.0	0.0	0.0	6.864870740204687E-4	5.72072561683724E-5
24-25	0.0	0.0	0.0	9.725233548623308E-4	1.144145123367448E-4
26-27	0.0	0.0	0.0	0.0010869378671990756	1.144145123367448E-4
28-29	0.0	0.0	0.0	0.0015445959165460547	1.144145123367448E-4
30-31	0.0	0.0	0.0	0.0029175700645869924	1.144145123367448E-4
32-33	0.0	0.0	0.0	0.006807663484036315	1.144145123367448E-4
34-35	0.0	0.0	0.0	0.011384243977506106	1.144145123367448E-4
36-37	0.0	0.0	0.0	0.020651819476782435	1.144145123367448E-4
38-39	0.0	0.0	0.0	0.0372991310217788	1.144145123367448E-4
40-41	0.0	0.0	0.0	0.05577707476416309	1.144145123367448E-4
42-43	0.0	0.0	0.0	0.07322528789551666	1.7162176850511718E-4
44-45	0.0	0.0	0.0	0.09256134048042654	2.288290246734896E-4
46-47	0.0	0.0	0.0	0.11870505654937272	2.288290246734896E-4
48-49	0.0	0.0	0.0	0.14633616127869659	3.4324353701023437E-4
50-51	0.0	0.0	0.0	0.17814339570831164	3.4324353701023437E-4
52-53	0.0	0.0	0.0	0.2094929720885797	4.004507931786068E-4
54-55	0.0	0.0	0.0	0.24124299926202641	4.576580493469792E-4
56-57	0.0	0.0	0.0	0.2764254618055754	4.576580493469792E-4
58-59	0.0	0.0	0.0	0.31824396606465566	4.576580493469792E-4
60-61	0.0	0.0	0.0	0.3669845483201089	4.576580493469792E-4
62-63	0.0	0.0	0.0	0.4161827886249092	4.576580493469792E-4
64-65	0.0	0.0	0.0	0.46377922575699504	4.576580493469792E-4
66-67	0.0	0.0	0.0	0.5242472955269646	5.148653055153515E-4
68-69	0.0	0.0	0.0	0.593124831953685	5.720725616837239E-4
70-71	0.0	0.0	0.0	0.6649771457011607	5.720725616837239E-4
72-73	0.0	0.0	0.0	0.7527330766634439	6.864870740204687E-4
74-75	0.0	0.0	0.0	0.8434637849464826	6.864870740204687E-4
76-77	0.0	0.0	0.0	0.93247827554447	6.864870740204687E-4
78-79	0.0	0.0	0.0	1.035050885854362	6.864870740204687E-4
80-81	0.0	0.0	0.0	1.1506667505706423	6.864870740204687E-4
82-83	0.0	0.0	0.0	1.2849321807978122	6.864870740204687E-4
84-85	0.0	0.0	0.0	1.4205705851730233	6.864870740204687E-4
86-87	0.0	0.0	0.0	1.5687945859052763	6.864870740204687E-4
88-89	0.0	0.0	0.0	1.7209658873131468	6.864870740204687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	355	0.0	54.841515	6
CCGTGCG	365	0.0	53.366486	9
ACCGTGC	380	0.0	52.489124	8
GGTATCA	980	0.0	51.916847	1
AACCGTG	380	0.0	51.23352	7
GTATCAA	1655	0.0	37.925083	1
TGCGCGG	350	0.0	30.545116	12-13
ATCAACG	2105	0.0	29.776646	3
AATGCGG	260	0.0	29.22137	7
TCAACGC	2190	0.0	28.62093	4
ATGCGGG	300	0.0	26.91109	8
CGGCTTA	365	0.0	26.686296	16-17
AAATGCG	290	0.0	26.19847	6
GTACATG	3390	0.0	25.949121	1
GTCTAAT	740	0.0	25.70271	1
GTGCGCG	420	0.0	25.453535	10-11
TACATGG	3430	0.0	25.195978	2
CGCAGAG	2545	0.0	24.81804	8
ACGCAGA	2575	0.0	24.52609	7
ACATGGG	3440	0.0	24.156473	3
>>END_MODULE