FastQCFastQC Report
Fri 10 Feb 2017
SRR3224555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874015
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC23050.26372545093619676No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT13730.1570911254383506No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG13140.15034066921048267No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT13080.14965418213646217No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA11470.13123344565024628No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG10170.11635955904646947No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG10030.11475775587375503No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG10010.11452892684908153No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA9780.11189739306533641No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG9400.1075496415965401No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT8970.10262981756606007No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC8950.10240098854138659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.051.5701181
GTATCAA18900.047.7406541
CCGTGCG2700.047.4893889
AAACCGT2950.045.0746776
ACCGTGC3500.039.348358
ATCAACG23400.036.3273543
AACCGTG3600.035.6170437
GTCTAAT7050.035.2129671
TCAACGC24150.035.1991734
TCTAATG8350.031.849172
CAACGCA27350.031.2544425
TATCAAC27950.030.5835082
ACGCAGA28100.030.4202487
CGCAGAG28100.030.4202488
AACGCAG28450.030.3798566
CTAATGT9350.029.4586583
GTTATTA8800.028.6015628
TAATGTT10200.026.0726074
ATGCGGG1501.0616914E-625.3276718
GCAGAGT34450.024.9508849
Produced by FastQC (version 0.11.5)