##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874015
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.118429317574645	34.0	33.0	34.0	31.0	34.0
2	33.2528114506044	34.0	33.0	34.0	31.0	34.0
3	33.36354524807927	34.0	34.0	34.0	31.0	34.0
4	36.59770484488252	37.0	37.0	37.0	35.0	37.0
5	36.626213508921474	37.0	37.0	37.0	35.0	37.0
6	36.65865002316894	37.0	37.0	37.0	35.0	37.0
7	36.63228091051069	37.0	37.0	37.0	35.0	37.0
8	36.61738070856907	37.0	37.0	37.0	35.0	37.0
9	38.449605555968716	39.0	39.0	39.0	37.0	39.0
10-11	38.46808464385623	39.0	39.0	39.0	37.0	39.0
12-13	38.4559109397436	39.0	39.0	39.0	37.0	39.0
14-15	40.03002751669022	41.0	40.0	41.0	38.0	41.0
16-17	40.03657202679588	41.0	40.0	41.0	38.0	41.0
18-19	40.02969457045931	41.0	40.0	41.0	38.0	41.0
20-21	40.03003266534327	41.0	40.0	41.0	38.0	41.0
22-23	39.94603868354662	41.0	40.0	41.0	38.0	41.0
24-25	39.89341887725039	41.0	40.0	41.0	38.0	41.0
26-27	39.8228342762996	41.0	40.0	41.0	38.0	41.0
28-29	39.784552324616854	41.0	40.0	41.0	37.5	41.0
30-31	39.712487199876435	41.0	40.0	41.0	37.0	41.0
32-33	39.650167331224296	41.0	40.0	41.0	37.0	41.0
34-35	39.55790289640338	41.0	40.0	41.0	36.5	41.0
36-37	39.43462526386847	41.0	39.5	41.0	36.0	41.0
38-39	39.32145043277289	41.0	39.0	41.0	36.0	41.0
40-41	39.180769780839	41.0	39.0	41.0	35.0	41.0
42-43	39.07038952420725	41.0	39.0	41.0	35.0	41.0
44-45	38.80543754969881	40.5	38.5	41.0	35.0	41.0
46-47	38.55121651230242	40.0	38.0	41.0	35.0	41.0
48-49	38.43901363248915	40.0	38.0	41.0	35.0	41.0
50-51	38.266068660148854	40.0	37.5	41.0	35.0	41.0
52-53	38.01437503932999	40.0	37.0	41.0	34.0	41.0
54-55	37.82113236042859	40.0	36.5	41.0	34.0	41.0
56-57	37.5893920584887	39.5	35.5	41.0	34.0	41.0
58-59	37.32032631018919	39.0	35.0	41.0	33.0	41.0
60-61	37.06204756211278	39.0	35.0	41.0	33.0	41.0
62-63	36.83483750278886	38.0	35.0	41.0	33.0	41.0
64-65	36.55430455999039	37.5	35.0	40.0	33.0	41.0
66-67	36.25084466513732	37.0	35.0	40.0	32.0	41.0
68-69	35.96883405891204	36.5	35.0	39.5	32.0	41.0
70-71	35.656235304886074	36.0	35.0	39.0	32.0	41.0
72-73	35.33670360348506	35.5	35.0	39.0	31.0	41.0
74-75	35.01403808859116	35.0	35.0	38.5	31.0	40.0
76-77	33.180334433619564	34.5	32.0	35.5	28.5	38.5
78-79	34.519133538898075	35.0	34.5	37.0	31.0	39.0
80-81	34.45896523515043	35.0	35.0	36.5	32.0	39.0
82-83	34.2519499093265	35.0	35.0	36.0	31.0	37.0
84-85	34.046262935990804	35.0	35.0	36.0	31.0	37.0
86-87	33.844796141942645	35.0	34.0	35.0	31.0	37.0
88-89	33.68184356103728	35.0	34.0	35.0	31.0	36.0
90-91	33.54597804385509	35.0	34.0	35.0	31.0	36.0
92-93	33.45153000806622	35.0	34.0	35.0	31.0	36.0
94-95	33.344508389444115	35.0	34.0	35.0	30.0	36.0
96-97	33.29063288387499	35.0	34.0	35.0	30.0	35.0
98-99	33.254916105558834	35.0	34.0	35.0	30.0	35.0
100-101	32.395146536386676	34.5	32.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	18.0
15	45.0
16	160.0
17	361.0
18	667.0
19	1093.0
20	1610.0
21	2272.0
22	3282.0
23	3153.0
24	2644.0
25	2740.0
26	3486.0
27	4735.0
28	5887.0
29	6863.0
30	7338.0
31	8680.0
32	10376.0
33	14508.0
34	23694.0
35	45977.0
36	117147.0
37	241579.0
38	287105.0
39	78562.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28159018228885	19.35873590991542	13.763854435603456	23.595819472192268
2	15.729363912518664	19.980435118390417	39.40939228731772	24.880808681773196
3	18.607689799374153	26.881232015468843	29.09698346138224	25.414094723774767
4	12.462943999816936	17.399129305561118	39.65538348884172	30.48254320578022
5	13.472995314725718	38.27520122652357	34.83624422921803	13.415559229532672
6	29.321007076537587	37.545465466839815	19.51556895476622	13.617958501856375
7	26.580665091560213	31.455981876741244	23.314245178858485	18.649107852840054
8	25.940172651499115	34.20765089843996	20.613261786124955	19.238914663935976
9	26.322660366240854	15.522616888726166	20.815317814911644	37.33940493012134
10-11	25.80647929383363	25.28417704501639	27.986190168360952	20.923153492789027
12-13	26.399947369324323	23.22940681796079	28.108613696561275	22.262032116153613
14-15	23.624937787108916	24.971196146519226	25.475249280618755	25.928616785753107
16-17	23.23432664199127	27.17087235344931	26.524315944234367	23.07048506032505
18-19	23.01917015154202	26.745765232862134	27.611253811433446	22.6238108041624
20-21	24.485086192249362	25.249726689362937	27.27431349116224	22.990873627225458
22-23	24.437817218227835	25.85920659402016	26.577624358698586	23.125351829053415
24-25	23.88434026077445	25.6828281533892	26.89757120039542	23.53526038544093
26-27	23.45653162623463	26.67988180744224	26.98234262683818	22.881243939484953
28-29	23.370666184583367	26.59171873185394	27.257977687247315	22.77963739631538
30-31	23.497062373785045	25.652255993958846	27.34269254752547	23.50798908473064
32-33	23.548263270533006	25.829006073704814	27.583441216869076	23.039289438893107
34-35	24.007485301428694	25.800490743776027	26.863845204607202	23.328178750188076
36-37	23.338003424479247	25.839086088525022	27.353213924690063	23.46969656230567
38-39	23.40091417195357	26.057619148412787	26.853772532508025	23.687694147125622
40-41	23.78935144133682	25.8837662969171	27.140495300423908	23.186386961322174
42-43	23.76332213978021	25.978101062338748	27.176535871809982	23.082040926071063
44-45	23.913263621857677	25.76156936066792	26.805410696784833	23.51975632068957
46-47	23.75270528690355	25.630432044338075	26.541297850656715	24.07556481810166
48-49	23.47353005114358	25.658916946030363	26.92926854383817	23.938284458987884
50-51	23.74478698878166	25.86105501621826	26.50211952884104	23.892038466159047
52-53	24.124414340714974	26.125066503435296	26.021635784282875	23.72888337156685
54-55	23.88746188566558	25.575705222450416	26.497314119322894	24.03951877256111
56-57	24.062246123920133	26.017849991418274	26.007323073402368	23.912580811259225
58-59	24.358884110100984	25.93662812159297	26.285737178111436	23.418750590194616
60-61	23.912496108700033	26.22690399018477	26.195888040432898	23.664711860682306
62-63	23.88528800993118	25.812200019450465	26.58009301899853	23.722418951619822
64-65	24.117149019181593	25.660829619628956	26.242970658398313	23.97905070279114
66-67	23.643701767132143	25.856364021212453	26.1889098013192	24.311024410336206
68-69	24.00044621659811	25.879189716423628	26.493080782366434	23.62728328461182
70-71	24.40718980795524	25.611059306762467	26.238622906929514	23.743127978352774
72-73	23.97212862479477	25.604137228766096	26.60234664164803	23.821387504791108
74-75	23.99535477079913	25.44201186478493	26.426148292649437	24.136485071766504
76-77	23.910513916841055	25.189899921397053	26.213797735273957	24.685788426487935
78-79	23.854990874711795	25.151983248373753	26.324139390922767	24.66888648599168
80-81	23.57127001526389	25.795690410752	26.337135194139766	24.29590437984434
82-83	23.61301564799925	25.927006514231255	26.537354571088756	23.92262326668074
84-85	23.742028715217966	25.524072464821085	26.67550728177973	24.05839153818122
86-87	23.567044043866524	26.150352110661718	26.355325709512993	23.927278135958765
88-89	23.97024078534121	25.516552919572316	25.921179842451213	24.592026452635253
90-91	23.926076783579227	25.65247736022837	26.00229973169797	24.419146124494432
92-93	23.84960432578636	25.73847459052453	26.46168913673629	23.950231946952822
94-95	23.839808241277325	25.640120592896004	26.2068156724999	24.313255493326775
96-97	23.728826164310682	26.144516970532543	26.29285538577713	23.833801479379645
98-99	22.71585727933731	26.421457297643634	26.615447103310586	24.247238319708472
100-101	23.55093640784619	25.79619712222391	26.29813348141067	24.35473298851924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	4.5
4	3.5
5	2.0
6	5.0
7	13.0
8	31.0
9	47.0
10	63.0
11	73.5
12	66.5
13	74.0
14	94.0
15	114.0
16	137.5
17	153.5
18	175.5
19	202.5
20	242.5
21	305.0
22	387.0
23	516.5
24	712.5
25	997.5
26	1459.5
27	2080.0
28	2893.0
29	3958.0
30	5284.0
31	7100.0
32	9250.5
33	11417.5
34	13568.0
35	15642.0
36	18193.0
37	20926.5
38	22535.5
39	23594.5
40	24396.0
41	25041.0
42	25819.0
43	27002.0
44	28905.0
45	31217.5
46	33037.5
47	34416.0
48	35933.5
49	38351.0
50	41475.0
51	40335.0
52	37290.0
53	37756.0
54	38595.5
55	36463.5
56	30540.0
57	26010.5
58	24656.0
59	23348.0
60	19201.5
61	14053.5
62	11331.0
63	8218.5
64	5067.0
65	3307.5
66	2618.0
67	2210.0
68	1677.0
69	1079.0
70	659.5
71	473.5
72	358.5
73	275.0
74	203.0
75	140.0
76	89.0
77	53.5
78	30.0
79	18.5
80	10.0
81	7.5
82	5.5
83	5.0
84	4.0
85	1.5
86	1.5
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4664679667969085
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0010869378671990756
22-23	0.0012585596357041927
24-25	3.4324353701023437E-4
26-27	0.002002253965893034
28-29	0.0034896426262707165
30-31	0.0011441451233674478
32-33	1.7162176850511718E-4
34-35	0.004061715187954441
36-37	0.0015445959165460547
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009095953730771211
46-47	0.08295052144413997
48-49	5.720725616837239E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0074369433018884115
58-59	0.04147526072206999
60-61	0.03100633284325784
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.2882902467348957E-4
78-79	0.008009015863572135
80-81	0.006521627203194453
82-83	6.292798178520963E-4
84-85	0.0021166684782297786
86-87	0.0
88-89	0.0
90-91	0.0
92-93	5.720725616837239E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.068362671121205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	874015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.396636646730016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9502576162224	43.39735718654364
2	14.202539624802457	16.01950933361539
3	4.41110620305092	7.46314661230799
4	1.7821942582834487	4.020390480732006
5	0.8422652012625504	2.3750462257894496
6	0.4561951688170562	1.5436723905461507
7	0.25563665636127003	1.009193333567407
8	0.17879391958367324	0.8066700573924069
9	0.12285167384897067	0.6235579090352685
>10	0.6286706917381465	6.6410733664251085
>50	0.0811853804739138	3.26637326805531
>100	0.0805719534807359	9.183443983072454
>500	0.006103935848302073	2.449392679209649
>1k	0.0016277162262138861	1.2011731737077955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	2305	0.26372545093619676	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1373	0.1570911254383506	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1314	0.15034066921048267	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1308	0.14965418213646217	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1147	0.13123344565024628	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1017	0.11635955904646947	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1003	0.11475775587375503	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1001	0.11452892684908153	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	978	0.11189739306533641	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	940	0.1075496415965401	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	897	0.10262981756606007	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	895	0.10240098854138659	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.144145123367448E-4	0.0
5	0.0	0.0	0.0	1.144145123367448E-4	0.0
6	0.0	0.0	0.0	1.144145123367448E-4	0.0
7	0.0	0.0	0.0	1.144145123367448E-4	0.0
8	0.0	0.0	0.0	1.144145123367448E-4	1.144145123367448E-4
9	0.0	0.0	0.0	1.144145123367448E-4	1.144145123367448E-4
10-11	0.0	0.0	0.0	1.144145123367448E-4	1.144145123367448E-4
12-13	0.0	0.0	0.0	1.144145123367448E-4	1.144145123367448E-4
14-15	0.0	0.0	0.0	1.144145123367448E-4	1.144145123367448E-4
16-17	0.0	0.0	0.0	1.144145123367448E-4	1.144145123367448E-4
18-19	0.0	0.0	0.0	1.7162176850511718E-4	1.144145123367448E-4
20-21	0.0	0.0	0.0	2.288290246734896E-4	1.144145123367448E-4
22-23	0.0	0.0	0.0	3.4324353701023437E-4	1.144145123367448E-4
24-25	0.0	0.0	0.0	6.292798178520963E-4	1.144145123367448E-4
26-27	0.0	0.0	0.0	7.436943301888412E-4	1.144145123367448E-4
28-29	0.0	0.0	0.0	0.0011441451233674478	1.144145123367448E-4
30-31	0.0	0.0	0.0	0.0025171192714083853	1.144145123367448E-4
32-33	0.0	0.0	0.0	0.00669324897169957	1.144145123367448E-4
34-35	0.0	0.0	0.0	0.011269829465169363	1.144145123367448E-4
36-37	0.0	0.0	0.0	0.020823441245287552	1.144145123367448E-4
38-39	0.0	0.0	0.0	0.037527960046452286	1.144145123367448E-4
40-41	0.0	0.0	0.0	0.05634914732584681	1.144145123367448E-4
42-43	0.0	0.0	0.0	0.07431222576271575	1.144145123367448E-4
44-45	0.0	0.0	0.0	0.09364827834762562	1.144145123367448E-4
46-47	0.0	0.0	0.0	0.11973478716040342	1.7162176850511718E-4
48-49	0.0	0.0	0.0	0.1474230991458957	2.288290246734896E-4
50-51	0.0	0.0	0.0	0.17911591906317398	2.288290246734896E-4
52-53	0.0	0.0	0.0	0.21103756800512577	2.8603628084186196E-4
54-55	0.0	0.0	0.0	0.2435312895087613	3.4324353701023437E-4
56-57	0.0	0.0	0.0	0.2793430318701624	3.4324353701023437E-4
58-59	0.0	0.0	0.0	0.32196243771559985	3.4324353701023437E-4
60-61	0.0	0.0	0.0	0.3719615796067573	3.4324353701023437E-4
62-63	0.0	0.0	0.0	0.4217890997294097	3.4324353701023437E-4
64-65	0.0	0.0	0.0	0.46938553686149553	3.4324353701023437E-4
66-67	0.0	0.0	0.0	0.5300824356561387	3.4324353701023437E-4
68-69	0.0	0.0	0.0	0.5990171793390273	3.4324353701023437E-4
70-71	0.0	0.0	0.0	0.6721280527222073	3.4324353701023437E-4
72-73	0.0	0.0	0.0	0.7619434449065519	3.4324353701023437E-4
74-75	0.0	0.0	0.0	0.854275956362305	3.4324353701023437E-4
76-77	0.0	0.0	0.0	0.9447778356206702	3.4324353701023437E-4
78-79	0.0	0.0	0.0	1.0494099071526233	3.4324353701023437E-4
80-81	0.0	0.0	0.0	1.1676000983964805	3.4324353701023437E-4
82-83	0.0	0.0	0.0	1.3030668810031862	3.4324353701023437E-4
84-85	0.0	0.0	0.0	1.4409935756251322	3.4324353701023437E-4
86-87	0.0	0.0	0.0	1.593050462520666	3.4324353701023437E-4
88-89	0.0	0.0	0.0	1.7470523961259246	3.4324353701023437E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	935	0.0	51.570118	1
GTATCAA	1890	0.0	47.740654	1
CCGTGCG	270	0.0	47.489388	9
AAACCGT	295	0.0	45.074677	6
ACCGTGC	350	0.0	39.34835	8
ATCAACG	2340	0.0	36.327354	3
AACCGTG	360	0.0	35.617043	7
GTCTAAT	705	0.0	35.212967	1
TCAACGC	2415	0.0	35.199173	4
TCTAATG	835	0.0	31.84917	2
CAACGCA	2735	0.0	31.254442	5
TATCAAC	2795	0.0	30.583508	2
ACGCAGA	2810	0.0	30.420248	7
CGCAGAG	2810	0.0	30.420248	8
AACGCAG	2845	0.0	30.379856	6
CTAATGT	935	0.0	29.458658	3
GTTATTA	880	0.0	28.601562	8
TAATGTT	1020	0.0	26.072607	4
ATGCGGG	150	1.0616914E-6	25.327671	8
GCAGAGT	3445	0.0	24.950884	9
>>END_MODULE