FastQCFastQC Report
Fri 10 Feb 2017
SRR3224554_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224554_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071976
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA29830.2782711553243729No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA25290.2359194608834526No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21950.20476204691149805No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG19240.17948163018575045No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC17290.16129092442368115No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA16190.15102950066046256No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC15530.14487264640253142No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC15250.1422606476264394No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC14530.13554407934505996No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG13840.12910736807540465No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA13760.12836108271080696No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA13110.12229751412345052No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG11950.11147637633678366No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT11720.10933080591356521No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC11510.10737180683149622No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA11130.10382695134965708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.051.2139471
GTATCAA16600.043.5450741
CCGTGCG3500.042.0854649
AACCGTG3800.041.231047
AAACCGT3700.039.779016
ACCGTGC3950.036.0627378
ATCAACG19950.034.745933
TCAACGC21950.031.7963124
GCCCATA13750.030.4357171
CCCATAA14000.030.182552
CCATAAT13900.029.3749853
CGCAGAG25050.027.2953878
ACGCAGA25800.026.3154227
TATCAAC26900.026.2983172
GTACATG42150.025.949791
CATAATA16150.025.2824944
TACATGG43550.024.965562
GTCTAAT8200.024.9378011
ATAATAA16900.024.722365
ACATGGG43800.024.2810753
Produced by FastQC (version 0.11.5)