##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224554_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071976
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04988917662336	34.0	31.0	34.0	30.0	34.0
2	32.22092099076845	34.0	31.0	34.0	30.0	34.0
3	32.36377959954327	34.0	31.0	34.0	31.0	34.0
4	35.67408878556983	37.0	35.0	37.0	35.0	37.0
5	35.68779524914737	37.0	37.0	37.0	35.0	37.0
6	35.782927043142756	37.0	37.0	37.0	35.0	37.0
7	35.738776800973156	37.0	37.0	37.0	35.0	37.0
8	35.77314510772629	37.0	37.0	37.0	35.0	37.0
9	37.482799055202726	39.0	38.0	39.0	35.0	39.0
10-11	37.54628461831235	39.0	38.0	39.0	35.0	39.0
12-13	37.53798639148637	39.0	38.0	39.0	35.0	39.0
14-15	38.99102778420412	41.0	39.0	41.0	36.0	41.0
16-17	38.81843623364702	41.0	39.0	41.0	35.5	41.0
18-19	38.89821647126428	41.0	39.0	41.0	35.5	41.0
20-21	38.9633737135906	41.0	39.0	41.0	36.0	41.0
22-23	38.91059781189131	41.0	39.0	41.0	35.0	41.0
24-25	38.840564061135694	41.0	39.0	41.0	35.0	41.0
26-27	38.65314102181392	41.0	39.0	41.0	35.0	41.0
28-29	38.57356321410181	41.0	39.0	41.0	34.5	41.0
30-31	38.48895264446219	40.0	39.0	41.0	34.0	41.0
32-33	38.298518810122616	40.0	38.0	41.0	34.0	41.0
34-35	38.282158835645575	40.0	38.0	41.0	34.0	41.0
36-37	38.27223277386807	40.0	38.0	41.0	34.0	41.0
38-39	38.155204500847034	40.0	38.0	41.0	33.5	41.0
40-41	38.05165600722404	40.0	38.0	41.0	33.0	41.0
42-43	37.89866704105316	40.0	38.0	41.0	33.0	41.0
44-45	37.71178412576401	40.0	37.5	41.0	32.5	41.0
46-47	37.52074066956723	40.0	37.0	41.0	32.0	41.0
48-49	37.29778465189519	40.0	36.5	41.0	31.5	41.0
50-51	36.00536112748793	38.5	34.5	40.0	29.5	40.5
52-53	36.3532504459055	39.0	35.0	40.0	30.5	40.5
54-55	36.80649146995828	39.0	35.0	41.0	31.0	41.0
56-57	36.63750214557042	39.0	35.0	41.0	31.0	41.0
58-59	36.485850895915576	39.0	35.0	41.0	31.0	41.0
60-61	36.232399326104314	38.0	35.0	41.0	30.5	41.0
62-63	35.95443927849131	37.5	35.0	41.0	30.0	41.0
64-65	35.700116886945224	37.0	35.0	40.0	30.0	41.0
66-67	35.465911083830235	36.5	35.0	40.0	29.0	41.0
68-69	35.163385654156436	36.0	35.0	39.5	29.0	41.0
70-71	34.88311398762659	35.5	34.0	39.0	29.0	41.0
72-73	34.56352287737785	35.0	34.0	39.0	28.5	41.0
74-75	34.29784715329448	35.0	34.0	38.5	28.0	40.0
76-77	33.97121530705911	35.0	34.0	37.0	28.0	39.5
78-79	33.74520185153399	35.0	34.0	37.0	28.0	39.0
80-81	33.442748251826536	35.0	34.0	36.0	27.0	39.0
82-83	33.17847507780024	35.0	34.0	36.0	27.0	37.5
84-85	32.94856881124204	35.0	33.0	35.5	26.5	37.0
86-87	32.71809443495003	35.0	33.0	35.0	26.0	37.0
88-89	32.560627756591565	35.0	33.0	35.0	26.0	36.0
90-91	32.38813835384374	35.0	33.0	35.0	25.5	36.0
92-93	32.24495044665179	35.0	33.0	35.0	25.0	36.0
94-95	32.10103864265618	35.0	33.0	35.0	25.0	35.5
96-97	31.961348015254075	35.0	33.0	35.0	25.0	35.0
98-99	31.917280797331284	35.0	33.0	35.0	25.0	35.0
100-101	30.70714176436786	34.0	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	9.0
7	110.0
8	411.0
9	431.0
10	583.0
11	1324.0
12	2357.0
13	2596.0
14	2751.0
15	2829.0
16	2934.0
17	3134.0
18	3429.0
19	3875.0
20	4625.0
21	5772.0
22	6589.0
23	6523.0
24	6409.0
25	6659.0
26	6851.0
27	7354.0
28	7997.0
29	9339.0
30	11390.0
31	14468.0
32	18726.0
33	26562.0
34	44094.0
35	76791.0
36	158198.0
37	257429.0
38	282315.0
39	87095.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06144842174501	20.029786152615724	13.902228916112927	24.006536509526335
2	15.57991969969477	20.520981813025667	38.968036597834285	24.931061889445285
3	18.364420812052522	27.557545651717625	29.29508617271858	24.782947363511276
4	12.472480727180459	17.733792547594348	40.13578662208855	29.65794010313664
5	13.24180765241013	39.549672753867625	34.481928699896265	12.726590893825982
6	28.38188541534512	38.65991402792599	19.917423524407262	13.040777032321618
7	25.611487570617253	32.01965342507668	23.971898624596072	18.39696037970999
8	25.592550579490585	34.428662581998104	21.42799838802361	18.550788450487698
9	26.744628611088306	15.127577483077978	21.069221698993264	37.05857220684045
10-11	25.63028463323806	25.241936386635523	28.30576430815615	20.82201467197027
12-13	26.006599028336456	23.128969305282954	28.403807547930178	22.46062411845041
14-15	23.45474685788729	24.77584334043816	25.453352644525985	26.316057157148563
16-17	23.171787660602384	27.129823906270055	26.58606887377032	23.112319559357243
18-19	23.107022504804263	26.91217588430393	27.11251095801128	22.86829065288053
20-21	24.63456698182144	25.235666150585562	26.520082834283837	23.60968403330916
22-23	24.6597305102047	26.21273441733532	25.795650207491928	23.331884864968053
24-25	23.679427573897474	26.025271765244838	26.34916947272271	23.946131188134977
26-27	23.47599199982089	26.811794293902103	26.660998007418073	23.05121569885893
28-29	23.29459801338836	26.995893564781305	26.630493593140148	23.079014828690195
30-31	23.536347828682732	26.066441786010135	26.837214639133713	23.55999574617342
32-33	23.474312857750547	26.114763763367836	26.974997574572562	23.43592580430905
34-35	24.005015037650097	25.868069807533008	26.529371926237154	23.597543228579745
36-37	23.26156854914709	25.956386891621303	27.364608273259794	23.41743628597181
38-39	23.294956820592525	26.253564854938276	26.449427804049357	24.002050520419843
40-41	24.03580139628804	25.767424330414723	26.85697120150087	23.33980307179637
42-43	23.868484746997606	26.2167581755076	26.757181978596677	23.157575098898114
44-45	23.852473201944843	25.84685772715547	26.33572777029079	23.964941300608896
46-47	23.774133002977678	25.748104435173925	26.170175451689214	24.307587110159183
48-49	23.287321731083534	26.113364478309215	26.5623950536206	24.03691873698665
50-51	23.36857355015411	26.552460129704397	26.134213825682668	23.944752494458832
52-53	23.890693448360782	26.263087979581634	25.58112308484518	24.265095487212403
54-55	23.714290245304	25.65160040896438	26.128896542459906	24.505212803271714
56-57	23.66280759215113	26.281430872254074	26.01542665853837	24.040334877056424
58-59	24.206796270042645	26.208086612487214	26.153785867531376	23.431331249938772
60-61	23.88628214589944	26.4668644038541	25.969706837047823	23.67714661319863
62-63	23.45609776239561	26.095228941655442	26.478512997831867	23.97016029811708
64-65	24.437732975194734	25.562220359768766	25.75588026124954	24.24416640378696
66-67	23.54792389948432	25.978057330100125	25.68233791413081	24.79168085628475
68-69	23.56372717299641	26.190511727874505	26.718088837809802	23.52767226131928
70-71	24.06084651148906	25.66092897602185	26.363743218131745	23.914481294357337
72-73	23.99274797196952	25.786491488615415	26.630773450151867	23.589987089263193
74-75	23.873528884975038	25.57823122905737	26.48869937386658	24.059540512101016
76-77	23.69749882460055	25.376361037933687	25.79768576908438	25.12845436838138
78-79	23.936629524838064	24.991311284600332	26.37338850570423	24.698670684857376
80-81	23.487289869932763	26.09302171458761	26.105156158627054	24.314532256852576
82-83	23.385864619548734	26.3810812702709	26.340294467648224	23.89275964253214
84-85	23.43993490436279	25.41193332922753	26.953149057438313	24.194982708971366
86-87	23.597485732634137	26.461599923832708	26.11221980984313	23.828694533690026
88-89	23.72325797437394	25.86122025430091	25.962190341679232	24.45333142964592
90-91	23.35635312730882	25.634902273931502	26.324376665149217	24.684367933610453
92-93	23.361259245473292	25.603215094825426	26.785227019894236	24.250298639807042
94-95	23.810141271819518	25.378459965521614	26.61668731389509	24.19471144876378
96-97	23.781829070800093	26.101283983969793	26.5233083576498	23.593578587580318
98-99	22.843369784998146	26.435689514840703	26.57855765216763	24.142383047993526
100-101	23.662642753713474	25.643567082404118	26.20069913329311	24.493091030589298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	2.5
2	1.0
3	0.0
4	0.5
5	3.0
6	7.0
7	15.5
8	22.0
9	27.0
10	31.0
11	33.0
12	42.0
13	51.5
14	57.0
15	59.5
16	62.5
17	70.0
18	93.0
19	114.5
20	137.0
21	175.0
22	227.0
23	279.0
24	372.0
25	569.0
26	832.5
27	1172.5
28	1877.0
29	3229.5
30	5379.0
31	8632.0
32	13027.5
33	17503.5
34	21502.0
35	24795.0
36	27364.5
37	28570.0
38	28669.0
39	29258.0
40	29726.5
41	29487.0
42	29925.5
43	30878.5
44	31971.0
45	34928.5
46	38829.0
47	39735.0
48	41641.0
49	45837.0
50	49465.0
51	49541.5
52	47169.5
53	47003.0
54	48227.5
55	47962.5
56	40491.0
57	32992.0
58	30226.5
59	27709.0
60	22665.5
61	16933.0
62	13517.5
63	9680.0
64	5786.5
65	3710.0
66	3025.0
67	2529.5
68	1948.5
69	1325.5
70	782.5
71	489.0
72	365.5
73	301.5
74	229.0
75	165.5
76	124.0
77	91.0
78	64.5
79	41.5
80	29.0
81	25.5
82	18.5
83	15.0
84	14.5
85	11.5
86	10.0
87	7.5
88	4.0
89	4.5
90	5.0
91	5.0
92	5.0
93	6.0
94	3.5
95	2.5
96	6.5
97	6.0
98	4.5
99	3.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1567199265655201
2	0.0
3	9.328567057471435E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0376874109121846
16-17	0.07588789301253013
18-19	0.02532705956103495
20-21	0.040952409382299604
22-23	0.06805189668425413
24-25	0.017444420397471586
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02182884691448316
38-39	0.004944140540459861
40-41	0.0058303544109196475
42-43	0.003684783987701217
44-45	0.010867780621954223
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.664283528735718E-5
58-59	0.015625349821264657
60-61	0.01707127771517273
62-63	0.029524914736897095
64-65	0.047575691993104324
66-67	0.01707127771517273
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017117920550460086
80-81	0.06021590035597812
82-83	0.05140040448666761
84-85	0.030037985925058023
86-87	0.060635685873564334
88-89	0.03465562661850638
90-91	0.0
92-93	0.0030317842936782168
94-95	0.0
96-97	0.0
98-99	7.92928199885072E-4
100-101	0.04724919214609283
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1071976.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.88683625837302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99377198869468	37.653089593280946
2	14.99063329621461	15.25651803907575
3	5.125406920869621	7.824472282194728
4	2.2371123473699686	4.553582788488004
5	1.1592234981874248	2.949460816956093
6	0.6571158250076065	2.006312723396866
7	0.40960831086981214	1.4590569731710619
8	0.25648265706472406	1.0441272778532042
9	0.1935524431448091	0.8864344333546159
>10	0.7876600584556718	7.100375556974958
>50	0.08145886169172693	2.8840617149512364
>100	0.09443068838781189	10.213247344665973
>500	0.010018186551249052	3.402496114366118
>1k	0.003524917490254296	2.7667643412704375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2983	0.2782711553243729	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	2529	0.2359194608834526	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2195	0.20476204691149805	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1924	0.17948163018575045	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1729	0.16129092442368115	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1619	0.15102950066046256	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1553	0.14487264640253142	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1525	0.1422606476264394	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1453	0.13554407934505996	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1384	0.12910736807540465	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1376	0.12836108271080696	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1311	0.12229751412345052	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1195	0.11147637633678366	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1172	0.10933080591356521	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1151	0.10737180683149622	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1113	0.10382695134965708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	9.328567057471435E-5	0.0	0.0	0.0
5	0.0	9.328567057471435E-5	0.0	0.0	0.0
6	0.0	9.328567057471435E-5	0.0	0.0	0.0
7	0.0	9.328567057471435E-5	0.0	0.0	0.0
8	0.0	9.328567057471435E-5	0.0	0.0	0.0
9	0.0	9.328567057471435E-5	0.0	0.0	0.0
10-11	0.0	1.865713411494287E-4	0.0	0.0	0.0
12-13	0.0	1.865713411494287E-4	0.0	0.0	0.0
14-15	0.0	1.865713411494287E-4	0.0	0.0	0.0
16-17	0.0	1.865713411494287E-4	0.0	0.0	0.0
18-19	0.0	1.865713411494287E-4	0.0	4.664283528735718E-5	0.0
20-21	0.0	1.865713411494287E-4	0.0	1.865713411494287E-4	0.0
22-23	0.0	1.865713411494287E-4	0.0	2.798570117241431E-4	0.0
24-25	0.0	1.865713411494287E-4	0.0	6.063568587356433E-4	0.0
26-27	0.0	1.865713411494287E-4	0.0	9.328567057471436E-4	0.0
28-29	0.0	2.798570117241431E-4	0.0	0.0014459278939080726	0.0
30-31	0.0	2.798570117241431E-4	0.0	0.0030317842936782168	0.0
32-33	0.0	5.597140234482862E-4	0.0	0.0067165682813794345	0.0
34-35	0.0	5.597140234482862E-4	0.0	0.010401352269080653	0.0
36-37	0.0	5.597140234482862E-4	0.0	0.019543347985402658	0.0
38-39	0.0	5.597140234482862E-4	0.0	0.038433696276782316	0.0
40-41	4.664283528735718E-5	5.597140234482862E-4	0.0	0.06408725568482876	0.0
42-43	9.328567057471435E-5	5.597140234482862E-4	0.0	0.08488996022299006	0.0
44-45	9.328567057471435E-5	5.597140234482862E-4	0.0	0.1114297335014963	0.0
46-47	9.328567057471435E-5	6.063568587356433E-4	0.0	0.14729807383747395	0.0
48-49	9.328567057471435E-5	6.529996940230006E-4	0.0	0.1850787704202333	0.0
50-51	9.328567057471435E-5	6.529996940230006E-4	0.0	0.22444532340276274	0.0
52-53	9.328567057471435E-5	6.529996940230006E-4	0.0	0.2676832317141429	0.0
54-55	9.328567057471435E-5	8.395710351724292E-4	0.0	0.3098017119786264	0.0
56-57	9.328567057471435E-5	8.395710351724292E-4	0.0	0.36302118704150094	0.0
58-59	9.328567057471435E-5	0.0010261423763218579	0.0	0.4212314454801227	0.0
60-61	9.328567057471435E-5	0.0010261423763218579	0.0	0.48956319917610097	0.0
62-63	9.328567057471435E-5	0.0010261423763218579	0.0	0.5597140234482862	0.0
64-65	9.328567057471435E-5	0.0010261423763218579	0.0	0.6321037038142645	0.0
66-67	9.328567057471435E-5	0.001072785211609215	0.0	0.7143350224258752	0.0
68-69	9.328567057471435E-5	0.0011194280468965723	0.0	0.8051019798950723	0.0
70-71	9.328567057471435E-5	0.0011194280468965723	0.0	0.9063635753039248	0.0
72-73	9.328567057471435E-5	0.0011194280468965723	0.0	1.022877377851743	0.0
74-75	9.328567057471435E-5	0.0011194280468965723	0.0	1.1425162503638142	0.0
76-77	9.328567057471435E-5	0.0011194280468965723	0.0	1.2671459062516326	0.0
78-79	9.328567057471435E-5	0.0011194280468965723	0.0	1.4173358358769228	0.0
80-81	9.328567057471435E-5	0.0011194280468965723	0.0	1.5834309723352016	0.0
82-83	9.328567057471435E-5	0.0011194280468965723	0.0	1.7752263110368143	0.0
84-85	9.328567057471435E-5	0.0011660708821839296	0.0	1.9619375806921049	0.0
86-87	9.328567057471435E-5	0.0012127137174712866	0.0	2.1586770599341776	0.0
88-89	9.328567057471435E-5	0.0012127137174712866	0.0	2.3853612394307335	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	845	0.0	51.213947	1
GTATCAA	1660	0.0	43.545074	1
CCGTGCG	350	0.0	42.085464	9
AACCGTG	380	0.0	41.23104	7
AAACCGT	370	0.0	39.77901	6
ACCGTGC	395	0.0	36.062737	8
ATCAACG	1995	0.0	34.74593	3
TCAACGC	2195	0.0	31.796312	4
GCCCATA	1375	0.0	30.435717	1
CCCATAA	1400	0.0	30.18255	2
CCATAAT	1390	0.0	29.374985	3
CGCAGAG	2505	0.0	27.295387	8
ACGCAGA	2580	0.0	26.315422	7
TATCAAC	2690	0.0	26.298317	2
GTACATG	4215	0.0	25.94979	1
CATAATA	1615	0.0	25.282494	4
TACATGG	4355	0.0	24.96556	2
GTCTAAT	820	0.0	24.937801	1
ATAATAA	1690	0.0	24.72236	5
ACATGGG	4380	0.0	24.281075	3
>>END_MODULE