FastQCFastQC Report
Fri 10 Feb 2017
SRR3224554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071976
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT22500.20989275879310731No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA19070.1778957737859803No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA18350.17117920550460086No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG17150.15998492503563513No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG16740.15616021254207182No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG16510.1540146421188534No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC15870.14804435920207168No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA15670.1461786457905774No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG15050.14039493421494512No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC14890.13890236348574966No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT14710.13722322141540483No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG14070.1312529384986231No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG13890.12957379642827824No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA13050.12173780010000224No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT12910.12043180071195624No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT12870.12005865802965739No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA12390.1155809458420711No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC12160.11343537541885265No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG12100.11287566139540436No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC12090.11278237572482966No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT11630.1084912348783928No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA11180.10429337970253065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.050.1021161
AAACCGT2750.050.076356
CCGTGCG2850.048.3192869
GTATCAA18800.047.9827351
GCGTAAT601.4435773E-647.7288551
ACCGTGC3100.047.486198
AACCGTG3300.044.6082427
ATCAACG22950.037.6578983
TCAACGC24500.035.2754554
AATGCCG2151.8189894E-1228.7125849
CGCAGAG30250.028.5702088
AATGCGG2850.028.3250987
ACGCAGA30600.028.2434227
GTCTAAT7950.028.2170581
CAACGCA30650.028.1973485
AACGCAG31400.027.5238466
TATCAAC31900.027.3901562
ATGCGGG2950.027.3649238
GTACATG47050.026.375141
GTATATA7300.025.4989781
Produced by FastQC (version 0.11.5)