##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224554_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071976
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.163796577535315	34.0	33.0	34.0	31.0	34.0
2	33.29290301275402	34.0	34.0	34.0	31.0	34.0
3	33.40775446465219	34.0	34.0	34.0	31.0	34.0
4	36.62912695806622	37.0	37.0	37.0	35.0	37.0
5	36.66248031672351	37.0	37.0	37.0	35.0	37.0
6	36.6961424509504	37.0	37.0	37.0	36.0	37.0
7	36.668491645335344	37.0	37.0	37.0	35.0	37.0
8	36.65537754576595	37.0	37.0	37.0	35.0	37.0
9	38.498544743539036	39.0	39.0	39.0	37.0	39.0
10-11	38.516254561669285	39.0	39.0	39.0	37.5	39.0
12-13	38.49762541325552	39.0	39.0	39.0	37.0	39.0
14-15	40.094442879318194	41.0	40.0	41.0	38.0	41.0
16-17	40.10184229870818	41.0	40.0	41.0	38.0	41.0
18-19	40.092826238647135	41.0	40.0	41.0	38.0	41.0
20-21	40.093705456092295	41.0	40.0	41.0	38.0	41.0
22-23	40.01978495787219	41.0	40.0	41.0	38.0	41.0
24-25	39.97154227333448	41.0	40.0	41.0	38.0	41.0
26-27	39.902254807943464	41.0	40.0	41.0	38.0	41.0
28-29	39.87718195183474	41.0	40.0	41.0	38.0	41.0
30-31	39.81407093069248	41.0	40.0	41.0	38.0	41.0
32-33	39.766413613737626	41.0	40.0	41.0	37.0	41.0
34-35	39.689690347545096	41.0	40.0	41.0	37.0	41.0
36-37	39.56800711956238	41.0	40.0	41.0	37.0	41.0
38-39	39.45666507459122	41.0	40.0	41.0	36.0	41.0
40-41	39.324643928595414	41.0	39.0	41.0	36.0	41.0
42-43	39.22301432121615	41.0	39.0	41.0	35.0	41.0
44-45	38.974876303200816	41.0	39.0	41.0	35.0	41.0
46-47	38.744762476025585	40.5	38.0	41.0	35.0	41.0
48-49	38.64327186429547	40.0	38.0	41.0	35.0	41.0
50-51	38.47061874519579	40.0	37.5	41.0	35.0	41.0
52-53	38.2108983783219	40.0	37.0	41.0	35.0	41.0
54-55	38.01687444495026	40.0	36.5	41.0	34.5	41.0
56-57	37.78174697940999	39.5	35.5	41.0	34.0	41.0
58-59	37.5066391411748	39.0	35.0	41.0	34.0	41.0
60-61	37.25317544422636	39.0	35.0	41.0	34.0	41.0
62-63	37.04044726747614	38.5	35.0	41.0	33.0	41.0
64-65	36.76886376187527	37.5	35.0	41.0	33.0	41.0
66-67	36.50198092121465	37.0	35.0	40.0	33.0	41.0
68-69	36.2379390956514	36.5	35.0	40.0	33.0	41.0
70-71	35.95099470510534	36.0	35.0	39.0	32.5	41.0
72-73	35.65231637648604	35.5	35.0	39.0	32.0	41.0
74-75	35.34312335350791	35.0	35.0	38.5	32.0	40.5
76-77	33.52311059202818	34.5	32.5	36.5	29.0	39.0
78-79	34.825239091173685	35.0	34.5	37.0	32.0	39.0
80-81	34.75419272446398	35.0	35.0	36.5	32.0	39.0
82-83	34.5371449547378	35.0	35.0	36.0	32.0	38.0
84-85	34.3267512518937	35.0	35.0	36.0	32.0	37.0
86-87	34.13166106330739	35.0	35.0	35.5	32.0	37.0
88-89	33.97652559385658	35.0	35.0	35.0	32.0	36.5
90-91	33.824389258714746	35.0	34.0	35.0	31.5	36.0
92-93	33.72572800137316	35.0	34.0	35.0	31.0	36.0
94-95	33.62810594640179	35.0	34.0	35.0	31.0	36.0
96-97	33.576469062740216	35.0	34.0	35.0	31.0	35.5
98-99	33.54853793368508	35.0	34.0	35.0	31.0	35.0
100-101	32.718447521213164	34.5	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	7.0
14	22.0
15	66.0
16	131.0
17	329.0
18	586.0
19	945.0
20	1315.0
21	1870.0
22	2560.0
23	2646.0
24	2445.0
25	3152.0
26	4063.0
27	5489.0
28	6989.0
29	8071.0
30	8576.0
31	9408.0
32	11351.0
33	15183.0
34	25942.0
35	52594.0
36	142585.0
37	295707.0
38	346291.0
39	123613.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32668835727831	20.063815766656262	13.940857932117948	23.668637943947484
2	15.413871206071777	20.57825921475854	39.49164906677015	24.51622051239953
3	18.058706538206078	28.11284954140764	29.584430994723764	24.244012925662513
4	12.417535467211954	17.980253289252744	40.44931976089017	29.15289148264513
5	13.230426800600014	38.8296006627014	35.198829078262946	12.741143458435635
6	28.09232669388121	38.25505421763174	20.416315290640835	13.236303797846222
7	25.75766621640783	31.833548512280124	24.13692097584274	18.2718642954693
8	25.618483995910356	34.363642469607534	21.241613618215332	18.77625991626678
9	26.74481518242946	15.231777577109934	21.126219243714413	36.8971879967462
10-11	25.839477749501853	25.133491794592416	28.348629073785236	20.678401382120494
12-13	26.290280752554164	23.348750345156983	28.374562490205	21.986406412083852
14-15	23.571283312312964	25.093938670268738	25.66685261610335	25.667925401314957
16-17	23.013388359440885	27.36619103412763	26.654188153466123	22.966232452965365
18-19	23.068380262244677	26.86599326850601	27.58308021821384	22.48254625103547
20-21	24.577855475028613	25.502417518449725	26.840704984388513	23.079022022133145
22-23	24.696234284642816	26.150951387223976	26.10743301295476	23.045381315178446
24-25	24.031819846124783	25.88205387731495	26.472367528084913	23.61375874847536
26-27	23.51739137938353	26.95713946547122	26.692202600206073	22.833266554939183
28-29	23.379792777290863	26.823367198771198	27.14213548631755	22.654704537620397
30-31	23.56480023881321	25.966785373623736	27.101661197750865	23.366753189812194
32-33	23.582439304702962	26.026762788445794	27.38146045587881	23.009337450972435
34-35	24.150510570574042	25.96754663049542	26.580921657782447	23.30102114114809
36-37	23.18164920399999	26.024169891697728	27.303096636188044	23.491084268114243
38-39	23.35266834332112	26.241072561326	26.623590453517615	23.782668641835265
40-41	23.704075464376068	26.150632103703813	26.942487518377277	23.202804913542842
42-43	23.758647581662277	26.185101158981173	27.075139741934525	22.981111517422033
44-45	23.976529078128653	25.757606148825495	26.778196616317413	23.48766815672844
46-47	23.63397448016226	25.738532527640267	26.450212509715232	24.177280482482246
48-49	23.34752221618969	25.748297987063022	26.9385409611872	23.965638835560082
50-51	23.668113838369514	26.026375590498297	26.489399016395886	23.8161115547363
52-53	23.958652059374465	26.137152324305767	26.050956364694734	23.853239251625034
54-55	23.896477159936417	25.498471980715987	26.48804637417256	24.11700448517504
56-57	23.955803238863	26.058820483660384	26.052989653308156	23.932386624168466
58-59	24.41791504422301	25.862027935971703	26.281464130482966	23.43859288932232
60-61	23.818586376808483	26.387427617492833	26.213770254387974	23.580215751310714
62-63	23.5836903064994	25.90057986372829	26.63529780517474	23.880432024597564
64-65	24.065417509347224	25.687282177959208	26.21971014276439	24.027590169929177
66-67	23.663869340358364	25.87072844914438	26.151191817727266	24.31421039276999
68-69	23.87133667171653	26.01765338029956	26.66463614857049	23.44637379941342
70-71	24.258798704448605	25.697916744404726	26.455023246789107	23.58826130435756
72-73	23.91881907803906	25.766761569288864	26.596584251886235	23.717835100785837
74-75	23.879219310880096	25.541150175003917	26.62284416815302	23.956786345962968
76-77	23.818241606011348	25.08133370958677	26.21312580313394	24.887298881267945
78-79	23.872181152578804	25.0985173879458	26.412315793008993	24.616985666466398
80-81	23.358411585284795	25.955454344878287	26.4488719451186	24.237262124718317
82-83	23.380976346720114	26.032880722373996	26.739619885154887	23.846523045751006
84-85	23.57008522769676	25.373664119288925	27.09944101974715	23.956809633267163
86-87	23.45766136555296	26.251147413748072	26.496395441688993	23.794795779009977
88-89	23.86252117584722	25.64068598550714	25.8817361582722	24.61505668037344
90-91	23.755475868862735	25.71051030993231	26.127823757248297	24.406190063956657
92-93	23.749552228781244	25.597541362866334	26.66477607707635	23.98813033127607
94-95	23.74694023000515	25.51535668709001	26.372185571318763	24.36551751158608
96-97	23.785607140458367	26.17045530870094	26.49550922781853	23.548428323022158
98-99	22.454933692545357	26.492943871877728	26.856198273095664	24.19592416248125
100-101	23.3641897950106	25.846242312138944	26.384538443622663	24.405029449227793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	2.5
4	3.5
5	3.0
6	4.0
7	12.0
8	20.0
9	28.0
10	29.0
11	26.0
12	31.0
13	39.5
14	48.0
15	58.5
16	73.5
17	91.0
18	94.0
19	95.5
20	116.0
21	163.5
22	234.0
23	300.0
24	421.0
25	615.0
26	893.5
27	1288.0
28	2029.0
29	3432.0
30	5701.0
31	9073.0
32	13548.5
33	17873.0
34	21720.5
35	24920.0
36	27403.5
37	28723.5
38	28733.5
39	29174.5
40	29681.0
41	29567.5
42	30245.0
43	31432.0
44	32901.5
45	35577.5
46	38225.0
47	40072.5
48	42133.0
49	46394.5
50	51104.0
51	50172.5
52	46220.5
53	45779.0
54	47720.5
55	47269.0
56	39730.0
57	32732.5
58	30636.0
59	28774.0
60	23164.5
61	16450.5
62	12853.0
63	8775.0
64	5056.0
65	3197.0
66	2534.0
67	2075.5
68	1535.0
69	986.0
70	546.0
71	376.0
72	279.0
73	223.0
74	184.0
75	128.5
76	78.0
77	52.0
78	36.0
79	23.5
80	13.0
81	5.5
82	4.5
83	2.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48088763181265254
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.395710351724293E-4
22-23	0.0013992850586207154
24-25	3.264998470115003E-4
26-27	0.002098927587931073
28-29	0.0034982126465517888
30-31	7.92928199885072E-4
32-33	2.3321417643678592E-4
34-35	0.0035448554818391454
36-37	0.002098927587931073
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009002067210459937
46-47	0.07915289148264514
48-49	9.328567057471437E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.008162496175287507
58-59	0.039040053135517956
60-61	0.030364485772069523
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.264998470115003E-4
78-79	0.007835996328276007
80-81	0.006016925752069076
82-83	9.794995410345008E-4
84-85	0.0022388560937931447
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06767875400195528
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1071976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.66160506822509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.17545764913511	35.12173615740619
2	15.81816000074362	15.394741097243624
3	5.608832252091905	8.188043398356594
4	2.410620136543225	4.692185802159091
5	1.2780975123919656	3.1097138193349383
6	0.7161256518243801	2.0908694188981953
7	0.4211342899436338	1.4345149348547155
8	0.31085493258691965	1.2101359970443535
9	0.20057927697246566	0.8784458604813833
>10	0.8581475985358842	7.471238916773604
>50	0.0845702939109378	2.925069007711978
>100	0.1026227441666034	10.934524006541041
>500	0.010185403131476468	3.3175019515596524
>1k	0.004612258021800665	3.231279631634691
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2250	0.20989275879310731	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1907	0.1778957737859803	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1835	0.17117920550460086	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1715	0.15998492503563513	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1674	0.15616021254207182	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1651	0.1540146421188534	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1587	0.14804435920207168	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1567	0.1461786457905774	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1505	0.14039493421494512	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1489	0.13890236348574966	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1471	0.13722322141540483	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1407	0.1312529384986231	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1389	0.12957379642827824	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1305	0.12173780010000224	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1291	0.12043180071195624	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	1287	0.12005865802965739	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1239	0.1155809458420711	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1216	0.11343537541885265	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1210	0.11287566139540436	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1209	0.11278237572482966	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1163	0.1084912348783928	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1118	0.10429337970253065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.865713411494287E-4	0.0	0.0	0.0
6	0.0	1.865713411494287E-4	0.0	0.0	0.0
7	0.0	1.865713411494287E-4	0.0	0.0	0.0
8	0.0	1.865713411494287E-4	0.0	0.0	0.0
9	0.0	1.865713411494287E-4	0.0	0.0	0.0
10-11	0.0	1.865713411494287E-4	0.0	0.0	0.0
12-13	0.0	1.865713411494287E-4	0.0	0.0	0.0
14-15	0.0	2.798570117241431E-4	0.0	0.0	0.0
16-17	0.0	2.798570117241431E-4	0.0	0.0	0.0
18-19	0.0	2.798570117241431E-4	0.0	4.664283528735718E-5	0.0
20-21	0.0	2.798570117241431E-4	0.0	1.865713411494287E-4	0.0
22-23	0.0	2.798570117241431E-4	0.0	2.798570117241431E-4	0.0
24-25	0.0	3.731426822988574E-4	0.0	5.597140234482862E-4	0.0
26-27	0.0	4.664283528735718E-4	0.0	8.395710351724292E-4	0.0
28-29	0.0	4.664283528735718E-4	0.0	0.0012127137174712866	0.0
30-31	0.0	5.13071188160929E-4	0.0	0.0028452129525287882	0.0
32-33	4.664283528735718E-5	5.597140234482862E-4	0.0	0.006529996940230005	0.0
34-35	9.328567057471435E-5	5.597140234482862E-4	0.0	0.010354709433793293	0.0
36-37	9.328567057471435E-5	6.063568587356433E-4	0.0	0.01968327649126473	0.0
38-39	9.328567057471435E-5	6.529996940230006E-4	0.0	0.03857362478264439	0.0
40-41	9.328567057471435E-5	6.529996940230006E-4	0.0	0.06464696970827705	0.0
42-43	9.328567057471435E-5	6.529996940230006E-4	0.0	0.08568288842287514	0.0
44-45	9.328567057471435E-5	6.529996940230006E-4	0.0	0.11226930453666872	0.0
46-47	9.328567057471435E-5	6.529996940230006E-4	0.0	0.14907050157839355	0.0
48-49	9.328567057471435E-5	6.529996940230006E-4	0.0	0.18764412636103794	0.0
50-51	9.328567057471435E-5	6.529996940230006E-4	0.0	0.22715060784942948	0.0
52-53	9.328567057471435E-5	6.529996940230006E-4	0.0	0.270575087501959	0.0
54-55	9.328567057471435E-5	6.529996940230006E-4	0.0	0.3129267819428793	0.0
56-57	9.328567057471435E-5	6.529996940230006E-4	0.0	0.3665193996880527	0.0
58-59	9.328567057471435E-5	6.529996940230006E-4	0.0	0.42594237184414574	0.0
60-61	9.328567057471435E-5	6.529996940230006E-4	0.0	0.49558012492817005	0.0
62-63	9.328567057471435E-5	6.529996940230006E-4	0.0	0.5670835914236886	0.0
64-65	9.328567057471435E-5	7.929281998850721E-4	0.0	0.64012627148369	0.0
66-67	9.328567057471435E-5	8.395710351724292E-4	0.0	0.724503160518519	0.0
68-69	9.328567057471435E-5	8.395710351724292E-4	0.0	0.8169959028933483	0.0
70-71	9.328567057471435E-5	8.395710351724292E-4	0.0	0.9202631402195571	0.0
72-73	9.328567057471435E-5	8.395710351724292E-4	0.0	1.0385027276730077	0.0
74-75	9.328567057471435E-5	8.395710351724292E-4	0.0	1.1616398128316305	0.0
76-77	9.328567057471435E-5	8.395710351724292E-4	0.0	1.288228467801518	0.0
78-79	9.328567057471435E-5	8.395710351724292E-4	0.0	1.4406572535206013	0.0
80-81	9.328567057471435E-5	8.395710351724292E-4	0.0	1.6092244602491101	0.0
82-83	9.328567057471435E-5	8.395710351724292E-4	0.0	1.8034918692209527	0.0
84-85	9.328567057471435E-5	8.395710351724292E-4	0.0	1.9925352806406114	0.0
86-87	9.328567057471435E-5	8.395710351724292E-4	0.0	2.193192758046822	0.0
88-89	9.328567057471435E-5	8.395710351724292E-4	0.0	2.422395650648895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	905	0.0	50.102116	1
AAACCGT	275	0.0	50.07635	6
CCGTGCG	285	0.0	48.319286	9
GTATCAA	1880	0.0	47.982735	1
GCGTAAT	60	1.4435773E-6	47.728855	1
ACCGTGC	310	0.0	47.48619	8
AACCGTG	330	0.0	44.608242	7
ATCAACG	2295	0.0	37.657898	3
TCAACGC	2450	0.0	35.275455	4
AATGCCG	215	1.8189894E-12	28.712584	9
CGCAGAG	3025	0.0	28.570208	8
AATGCGG	285	0.0	28.325098	7
ACGCAGA	3060	0.0	28.243422	7
GTCTAAT	795	0.0	28.217058	1
CAACGCA	3065	0.0	28.197348	5
AACGCAG	3140	0.0	27.523846	6
TATCAAC	3190	0.0	27.390156	2
ATGCGGG	295	0.0	27.364923	8
GTACATG	4705	0.0	26.37514	1
GTATATA	730	0.0	25.498978	1
>>END_MODULE