FastQCFastQC Report
Fri 10 Feb 2017
SRR3224553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062104
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG32870.3094800509178009No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA22890.21551561805623556No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA18630.17540655152414453No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA15890.14960870121946626No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA14340.1350150267770388No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT13540.12748280770997944No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13430.12644712758825877No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA12630.11891490852119944No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC12580.11844414482950823No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG12410.11684354827775811No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11750.11062946754743415No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG11720.11034700933241941No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA11660.10978209290238997No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC10750.10121419371360997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.042.1449931
CCGTGCG2150.041.988179
GTATCAA13550.041.7646031
AAACCGT2300.039.2239466
AACCGTG3000.034.819857
ATCAACG16800.033.0675543
TCAACGC17400.031.9272964
ACCGTGC3200.031.1612768
GTACATG33700.026.952821
CGCAGAG21550.026.0004398
TCGCACC12750.025.7359141
TATACCG1301.0050275E-525.5670245
CGCACCA13200.025.1796462
ACGCAGA22600.025.001387
AGTACCG950.001229264124.9903265
TACATGG35200.024.9548282
TATCAAC23100.024.6657752
ATACCGT1551.4152301E-624.5066416
ACATGGG35650.024.3734533
GTCTAAT6100.024.1675451
Produced by FastQC (version 0.11.5)