##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224553_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062104
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20859445026099	34.0	31.0	34.0	31.0	34.0
2	32.370763126774776	34.0	31.0	34.0	31.0	34.0
3	32.510793669923096	34.0	31.0	34.0	31.0	34.0
4	35.79783335718536	37.0	37.0	37.0	35.0	37.0
5	35.80617811438428	37.0	37.0	37.0	35.0	37.0
6	35.908580515655714	37.0	37.0	37.0	35.0	37.0
7	35.871560600468506	37.0	37.0	37.0	35.0	37.0
8	35.906937550371715	37.0	37.0	37.0	35.0	37.0
9	37.63875948118075	39.0	39.0	39.0	35.0	39.0
10-11	37.712371387359426	39.0	39.0	39.0	35.0	39.0
12-13	37.70734786800539	39.0	39.0	39.0	35.0	39.0
14-15	39.20726030595874	41.0	40.0	41.0	36.0	41.0
16-17	39.04808898186995	41.0	40.0	41.0	36.0	41.0
18-19	39.11750591279197	41.0	40.0	41.0	36.0	41.0
20-21	39.17524507957789	41.0	40.0	41.0	36.0	41.0
22-23	39.13507057689266	41.0	40.0	41.0	36.0	41.0
24-25	39.07815195122134	41.0	39.5	41.0	36.0	41.0
26-27	38.90647478966278	41.0	39.0	41.0	35.0	41.0
28-29	38.828277174363336	41.0	39.0	41.0	35.0	41.0
30-31	38.75229120688746	41.0	39.0	41.0	35.0	41.0
32-33	38.56243550537424	41.0	39.0	41.0	35.0	41.0
34-35	38.561293903421884	40.0	39.0	41.0	35.0	41.0
36-37	38.55797125328593	40.0	39.0	41.0	35.0	41.0
38-39	38.43979732681545	40.0	38.0	41.0	34.5	41.0
40-41	38.35220656357569	40.0	38.0	41.0	34.0	41.0
42-43	38.20445737893841	40.0	38.0	41.0	34.0	41.0
44-45	38.02621730075398	40.0	38.0	41.0	33.0	41.0
46-47	37.851307875688256	40.0	37.5	41.0	33.0	41.0
48-49	37.64241213666458	40.0	37.0	41.0	33.0	41.0
50-51	36.36101973064785	38.5	35.5	40.0	30.5	40.5
52-53	36.6896984664402	39.0	35.5	40.0	31.5	40.5
54-55	37.14969626326612	39.0	35.5	41.0	32.0	41.0
56-57	36.98987010688219	39.0	35.0	41.0	32.0	41.0
58-59	36.8352990855886	39.0	35.0	41.0	32.0	41.0
60-61	36.57057783418573	39.0	35.0	41.0	31.0	41.0
62-63	36.278735415740826	38.0	35.0	41.0	31.0	41.0
64-65	36.01797564080354	37.0	35.0	40.5	31.0	41.0
66-67	35.76930884357841	37.0	35.0	40.0	30.5	41.0
68-69	35.448419363828776	36.0	35.0	39.5	30.0	41.0
70-71	35.14580257677214	36.0	35.0	39.0	30.0	41.0
72-73	34.81801970428508	35.0	34.5	39.0	29.0	41.0
74-75	34.52939166032705	35.0	34.0	37.5	29.0	40.0
76-77	34.19590501495145	35.0	34.0	37.0	29.0	39.0
78-79	33.95791749207234	35.0	34.0	37.0	29.0	39.0
80-81	33.65556856955628	35.0	34.0	36.0	29.0	39.0
82-83	33.40175773747204	35.0	34.0	36.0	29.0	37.0
84-85	33.182587110113516	35.0	34.0	35.5	28.0	37.0
86-87	32.975572542801835	35.0	34.0	35.0	27.0	36.5
88-89	32.82660031409354	35.0	34.0	35.0	27.0	36.0
90-91	32.6667741577096	35.0	33.0	35.0	27.0	36.0
92-93	32.53625209960606	35.0	33.0	35.0	26.5	36.0
94-95	32.41190269502798	35.0	33.0	35.0	26.0	35.5
96-97	32.28351837484841	35.0	33.0	35.0	25.0	35.0
98-99	32.2357448046519	35.0	33.0	35.0	25.0	35.0
100-101	31.0304937181293	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	96.0
8	319.0
9	337.0
10	497.0
11	1068.0
12	1720.0
13	2066.0
14	2175.0
15	2197.0
16	2428.0
17	2604.0
18	2855.0
19	3311.0
20	3721.0
21	4833.0
22	5658.0
23	5599.0
24	5520.0
25	5828.0
26	6202.0
27	6606.0
28	7303.0
29	8688.0
30	10162.0
31	13130.0
32	17242.0
33	24293.0
34	39437.0
35	70210.0
36	151583.0
37	269981.0
38	301369.0
39	83036.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33690678137909	18.152544019039947	12.698266018046086	24.81228318153487
2	16.490381356251365	19.081747173534794	38.38851939169799	26.039352078515854
3	20.301515013139028	24.589705518203036	27.73149120188908	27.377288266768858
4	12.740089482762517	16.046545347724894	38.10568456572991	33.10768060378268
5	14.040338799213636	38.22299887769936	34.073876004609716	13.662786318477288
6	31.656881058728715	36.16030068618516	18.45968003133403	13.723138223752098
7	28.14394823859057	30.439015388323554	21.65098709730874	19.766049275777135
8	26.238202661886216	34.59877752084542	19.929686735009	19.233333082259367
9	26.491285222539414	15.201901132092527	19.615310741697613	38.69150290367045
10-11	26.36676822608709	24.615762674841633	27.564438134118692	21.453030964952585
12-13	26.631808184509236	22.286000241031008	27.583221605417172	23.49896996904258
14-15	23.682346647377504	24.333355937822585	24.66225125643286	27.32204615836705
16-17	23.804072595366804	26.966564204030195	25.74761214947768	23.481751051125322
18-19	23.688408799819182	26.57490770737588	26.466605138250582	23.270078354554357
20-21	24.81656707758153	24.97339162999578	26.340826375911746	23.86921491651094
22-23	24.51648187036655	25.96870851739949	25.56623602486813	23.948573587365832
24-25	23.984307720848065	25.616204933782893	26.143879479077352	24.255607866291687
26-27	23.933108245520213	26.619474175786927	26.16095034007969	23.286467238613167
28-29	23.645377477158547	26.729774108750178	26.1681059481934	23.45674246589788
30-31	23.862917379089055	25.818940518066025	26.305757251643907	24.012384851201013
32-33	23.676542033548504	25.946988242206036	26.55530908460942	23.821160639636044
34-35	24.36112659400586	25.56421028449191	26.090571168171856	23.98409195333037
36-37	23.5802561563551	25.76565097813758	26.709816125108944	23.944276740398372
38-39	23.711756636005752	26.088393451513912	26.021258946167166	24.17859096631317
40-41	24.3302295669761	25.606072447160805	26.439984068441674	23.623713917421423
42-43	24.173430966479643	25.770734724891277	26.436933124056676	23.618901184572405
44-45	24.220524371089912	25.49317511553716	26.22947732383173	24.056823189541205
46-47	24.043455254852635	25.44538952870905	26.04208250792766	24.46907270851065
48-49	23.507114180908836	25.902218615126205	26.410031409353508	24.180635794611447
50-51	23.79625724034558	26.080167290585482	26.009741042308477	24.11383442676047
52-53	24.041807581931714	26.10370547517004	25.417944005483456	24.436542937414792
54-55	24.11684731438729	25.25774312120094	25.99213448023922	24.63327508417255
56-57	24.102641597546757	25.852753521667143	25.752998648436805	24.291606232349295
58-59	24.29956089839266	26.00196244424125	26.12809939535247	23.570377262013615
60-61	24.169711466023806	26.19754783787856	25.713095148410424	23.919645547687207
62-63	23.729225390911584	25.906633277234313	26.216951572452025	24.147189759402078
64-65	24.53552380970323	25.14487211127082	25.752242315090246	24.5673617639357
66-67	23.587449984791796	25.812932527377118	25.568424194883455	25.031193292947634
68-69	23.781898947748996	25.87623245934485	26.508138562701955	23.833730030204197
70-71	24.246683940555727	25.470857844429545	26.055687578617537	24.226770636397188
72-73	23.89954279430263	25.718244164413278	26.517365531059106	23.864847510224987
74-75	24.11002124085777	25.526455036418277	26.31084149951417	24.05268222320978
76-77	24.01130209471012	25.184492290773786	25.85467148226539	24.949534132250704
78-79	24.001832506461092	24.852450741972074	26.321045526862086	24.824671224704748
80-81	23.851534764715883	25.801955602113775	25.819430634249464	24.52707899892088
82-83	23.652339536868443	26.05498874810545	26.223696518176826	24.068975196849284
84-85	23.8116518441988	25.29631593471019	26.660193315244367	24.231838905846644
86-87	23.688340659319955	26.358384879012885	25.935264404371853	24.018010057295307
88-89	24.14047559510607	25.443777786882123	25.609068677658748	24.806677940353065
90-91	23.695278428477813	25.431831534388344	26.005315863606576	24.867574173527263
92-93	23.77876630716482	25.61367101932403	26.47698389421051	24.13057877930064
94-95	24.022223812357357	25.355332434488524	26.305945557120587	24.316498196033535
96-97	24.219097188222623	25.740464210661102	26.248465310365088	23.79197329075119
98-99	23.194705196695597	25.93630719575783	26.340083495120037	24.528904112426535
100-101	23.914947849111414	25.50019733786975	25.859597167000587	24.72525764601825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	4.5
4	5.0
5	4.0
6	7.5
7	13.0
8	21.0
9	28.0
10	32.0
11	35.5
12	37.0
13	41.0
14	46.0
15	50.5
16	57.0
17	75.5
18	99.0
19	114.0
20	137.0
21	175.5
22	228.5
23	278.0
24	361.0
25	545.5
26	744.0
27	1033.0
28	1454.0
29	2127.5
30	3341.0
31	5321.5
32	8285.0
33	11851.0
34	15719.0
35	19397.0
36	23208.5
37	26426.5
38	27744.0
39	28416.5
40	28702.0
41	29020.0
42	29773.0
43	30949.0
44	32320.0
45	35028.5
46	39048.5
47	40313.0
48	41510.5
49	45234.0
50	48746.5
51	48957.5
52	47850.5
53	48777.0
54	50758.5
55	49998.0
56	42228.0
57	35671.0
58	33737.0
59	31376.5
60	25794.0
61	19206.0
62	15425.5
63	11241.5
64	6819.5
65	4191.0
66	3141.5
67	2733.5
68	2110.5
69	1354.0
70	779.0
71	455.0
72	314.0
73	292.0
74	232.5
75	151.5
76	104.0
77	57.5
78	38.5
79	34.5
80	29.0
81	18.0
82	15.0
83	11.0
84	8.5
85	9.5
86	6.5
87	7.0
88	8.0
89	8.5
90	6.5
91	5.5
92	6.5
93	3.5
94	2.0
95	3.0
96	4.5
97	4.0
98	3.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15036192312617222
2	0.0
3	9.415273833824183E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.034648207708472996
16-17	0.06981425547780631
18-19	0.024856322921295845
20-21	0.038414317242002664
22-23	0.06261157099493081
24-25	0.01699456927005265
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02010160963521463
38-39	0.005366706085279785
40-41	0.005743317038632751
42-43	0.0031070403651619807
44-45	0.010168495740530118
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.707636916912091E-5
58-59	0.015346896349133419
60-61	0.017324103854236498
62-63	0.027398446856428373
64-65	0.045005008925679595
66-67	0.016665034685868805
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01605304188667023
80-81	0.05550303925039356
82-83	0.04754713286081212
84-85	0.029658112576546176
86-87	0.055173504666209704
88-89	0.031964854665833105
90-91	0.0
92-93	0.003059963995992859
94-95	0.0
96-97	0.0
98-99	5.64916430029451E-4
100-101	0.044534245233988384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1062104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.024339365135106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07996341954171	37.288568543103565
2	15.064886077462022	15.373517194270429
3	5.391974486803656	8.2536580818846
4	2.435643355616625	4.971083725976764
5	1.2803254664146366	3.266388054808266
6	0.7049813290931898	2.158272394904095
7	0.47237429767415906	1.6871810530325555
8	0.296167514285475	1.2089401406264462
9	0.2105456513769652	0.966865749094053
>10	0.8907957612430443	7.995129525795454
>50	0.07946387737580589	2.9013145103179983
>100	0.08112904062549971	8.768435198755396
>500	0.008952169514131797	3.017878685696117
>1k	0.0027975529731661865	2.1427671417342875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3287	0.3094800509178009	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2289	0.21551561805623556	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1863	0.17540655152414453	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1589	0.14960870121946626	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1434	0.1350150267770388	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1354	0.12748280770997944	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1343	0.12644712758825877	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1263	0.11891490852119944	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1258	0.11844414482950823	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1241	0.11684354827775811	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1175	0.11062946754743415	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1172	0.11034700933241941	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1166	0.10978209290238997	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1075	0.10121419371360997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.415273833824183E-5	0.0
8	0.0	0.0	0.0	2.824582150147255E-4	0.0
9	0.0	0.0	0.0	4.7076369169120916E-4	0.0
10-11	0.0	0.0	0.0	5.64916430029451E-4	0.0
12-13	0.0	0.0	0.0	8.473746450441765E-4	0.0
14-15	0.0	0.0	0.0	0.0010827564908897811	0.0
16-17	0.0	0.0	0.0	0.0012710619675662647	0.0
18-19	0.0	0.0	0.0	0.0014122910750736274	0.0
20-21	0.0	0.0	0.0	0.0015064438134118692	0.0
22-23	0.0	0.0	0.0	0.0016476729209192321	0.0
24-25	0.0	0.0	0.0	0.001741825659257474	0.0
26-27	0.0	0.0	0.0	0.0020242838742721993	0.0
28-29	0.0	0.0	0.0	0.0024950475659634083	0.0
30-31	0.0	0.0	0.0	0.00569624066946363	0.0
32-33	0.0	0.0	0.0	0.012710619675662647	0.0
34-35	0.0	0.0	0.0	0.021372671602780897	0.0
36-37	0.0	0.0	0.0	0.03554265872268629	0.0
38-39	0.0	0.0	0.0	0.06035190527481302	0.0
40-41	0.0	0.0	0.0	0.09283460000150645	0.0
42-43	0.0	0.0	0.0	0.12164533793300844	0.0
44-45	0.0	0.0	0.0	0.15436341450554747	0.0
46-47	0.0	0.0	0.0	0.19188328073333685	0.0
48-49	0.0	0.0	0.0	0.23128620172789105	0.0
50-51	0.0	0.0	0.0	0.2756792178543721	0.0
52-53	0.0	0.0	0.0	0.3232734270843533	0.0
54-55	0.0	0.0	0.0	0.371809163697717	0.0
56-57	0.0	0.0	0.0	0.43220814534169916	0.0
58-59	0.0	0.0	0.0	0.4991978186693582	0.0
60-61	0.0	0.0	0.0	0.572307419989003	0.0
62-63	0.0	0.0	0.0	0.6515369493006334	0.0
64-65	0.0	0.0	0.0	0.7250231615736312	0.0
66-67	0.0	0.0	0.0	0.8154568667475124	0.0
68-69	0.0	0.0	0.0	0.9185070388587182	0.0
70-71	0.0	0.0	0.0	1.0350681289214614	0.0
72-73	0.0	0.0	0.0	1.159867583588801	0.0
74-75	0.0	0.0	0.0	1.2830193653352213	0.0
76-77	0.0	0.0	0.0	1.4136092134103628	0.0
78-79	0.0	0.0	0.0	1.5674077114858809	0.0
80-81	0.0	0.0	0.0	1.7377770915089295	0.0
82-83	0.0	0.0	0.0	1.9279185465830089	0.0
84-85	0.0	0.0	0.0	2.1224381039898166	0.0
86-87	0.0	0.0	0.0	2.332398710484096	0.0
88-89	0.0	0.0	0.0	2.5661799597779504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	835	0.0	42.144993	1
CCGTGCG	215	0.0	41.98817	9
GTATCAA	1355	0.0	41.764603	1
AAACCGT	230	0.0	39.223946	6
AACCGTG	300	0.0	34.81985	7
ATCAACG	1680	0.0	33.067554	3
TCAACGC	1740	0.0	31.927296	4
ACCGTGC	320	0.0	31.161276	8
GTACATG	3370	0.0	26.95282	1
CGCAGAG	2155	0.0	26.000439	8
TCGCACC	1275	0.0	25.735914	1
TATACCG	130	1.0050275E-5	25.567024	5
CGCACCA	1320	0.0	25.179646	2
ACGCAGA	2260	0.0	25.00138	7
AGTACCG	95	0.0012292641	24.990326	5
TACATGG	3520	0.0	24.954828	2
TATCAAC	2310	0.0	24.665775	2
ATACCGT	155	1.4152301E-6	24.506641	6
ACATGGG	3565	0.0	24.373453	3
GTCTAAT	610	0.0	24.167545	1
>>END_MODULE