FastQCFastQC Report
Fri 10 Feb 2017
SRR3224553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062104
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG23820.22427182272169205No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA18400.17324103854236494No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT17300.16288423732515836No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA17160.16156609898842297No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA16480.15516371278142255No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG13970.13153137545852384No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG12840.12089211602630251No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG12760.12013889411959658No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT12170.1145838825576403No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG11960.11260667505253723No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11710.11025285659408118No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC11670.1098762456407282No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA11330.10667505253722799No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT11240.10582767789218382No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG11080.10432123407877195No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC10960.10319140121871305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1500.053.8217289
GGTATCA8600.044.3833731
GTATCAA14200.042.336111
AAACCGT2150.035.3412176
ATCAACG17300.034.313413
ACCGTGC2700.033.418728
TCAACGC17950.033.0708664
GTACATG39050.029.6902581
TATCACG650.00585003429.2244682
AACCGTG2800.028.8330697
TACATGG39200.028.8330692
TATACCG1354.23257E-728.142085
ATACCGT1903.965397E-1027.4940726
ACATGGG41100.027.269063
CGCAGAG22100.026.645848
ACGCAGA22350.026.560277
GTCTAAT5650.026.1783371
CAACGCA22750.026.0932755
GACCGCA9750.025.8149477
AACGCAG23200.025.5871566
Produced by FastQC (version 0.11.5)