##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062104
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.15850331041028	34.0	33.0	34.0	31.0	34.0
2	33.29299390643477	34.0	34.0	34.0	31.0	34.0
3	33.407524121931566	34.0	34.0	34.0	31.0	34.0
4	36.62181481286202	37.0	37.0	37.0	35.0	37.0
5	36.650553053185	37.0	37.0	37.0	35.0	37.0
6	36.68600814986103	37.0	37.0	37.0	36.0	37.0
7	36.66650064400473	37.0	37.0	37.0	35.0	37.0
8	36.651254491085616	37.0	37.0	37.0	35.0	37.0
9	38.49204691819257	39.0	39.0	39.0	37.0	39.0
10-11	38.51702281509155	39.0	39.0	39.0	37.5	39.0
12-13	38.50896286992611	39.0	39.0	39.0	37.0	39.0
14-15	40.11801104223315	41.0	40.0	41.0	38.0	41.0
16-17	40.12497363723327	41.0	40.0	41.0	38.0	41.0
18-19	40.11003960064174	41.0	40.0	41.0	38.0	41.0
20-21	40.10637423453824	41.0	40.0	41.0	38.0	41.0
22-23	40.03057939712119	41.0	40.0	41.0	38.0	41.0
24-25	39.997174947086165	41.0	40.0	41.0	38.0	41.0
26-27	39.93268832430722	41.0	40.0	41.0	38.0	41.0
28-29	39.90255097429254	41.0	40.0	41.0	38.0	41.0
30-31	39.84315660236662	41.0	40.0	41.0	38.0	41.0
32-33	39.802902540617495	41.0	40.0	41.0	38.0	41.0
34-35	39.728006391087874	41.0	40.0	41.0	37.0	41.0
36-37	39.613369312233075	41.0	40.0	41.0	37.0	41.0
38-39	39.509983014846	41.0	40.0	41.0	36.5	41.0
40-41	39.3793738654595	41.0	39.0	41.0	36.0	41.0
42-43	39.2823183981983	41.0	39.0	41.0	35.5	41.0
44-45	39.04585709120764	41.0	39.0	41.0	35.0	41.0
46-47	38.82103494573036	40.5	38.5	41.0	35.0	41.0
48-49	38.72569164601583	40.0	38.0	41.0	35.0	41.0
50-51	38.56665637263394	40.0	38.0	41.0	35.0	41.0
52-53	38.323725830992075	40.0	37.0	41.0	35.0	41.0
54-55	38.12898501465017	40.0	36.5	41.0	35.0	41.0
56-57	37.90181375835135	40.0	36.0	41.0	34.0	41.0
58-59	37.63299639206707	39.0	35.0	41.0	34.0	41.0
60-61	37.37471612949391	39.0	35.0	41.0	34.0	41.0
62-63	37.14705339590097	38.5	35.0	41.0	34.0	41.0
64-65	36.870139364883286	37.5	35.0	40.5	33.0	41.0
66-67	36.575082571951526	37.0	35.0	40.0	33.0	41.0
68-69	36.29542634242974	36.5	35.0	40.0	33.0	41.0
70-71	35.987433434013994	36.0	35.0	39.0	33.0	41.0
72-73	35.67300989356974	35.5	35.0	39.0	32.5	41.0
74-75	35.35029196764158	35.0	35.0	38.5	32.0	40.0
76-77	33.5165388700165	34.5	32.5	35.5	29.0	38.5
78-79	34.80962410460746	35.0	34.5	37.0	32.0	39.0
80-81	34.73735669953225	35.0	35.0	36.5	33.0	39.0
82-83	34.52885969735544	35.0	35.0	36.0	32.0	37.0
84-85	34.324000286224326	35.0	35.0	36.0	32.0	37.0
86-87	34.13813148241603	35.0	35.0	35.5	32.0	37.0
88-89	33.990923676024195	35.0	35.0	35.0	32.0	36.0
90-91	33.84749186520341	35.0	34.0	35.0	32.0	36.0
92-93	33.75987897607014	35.0	34.0	35.0	31.5	36.0
94-95	33.66222940503002	35.0	34.0	35.0	31.0	36.0
96-97	33.61575373033149	35.0	34.0	35.0	31.0	35.0
98-99	33.57633527413511	35.0	34.0	35.0	31.0	35.0
100-101	32.73944406574121	34.5	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	13.0
15	53.0
16	116.0
17	283.0
18	478.0
19	800.0
20	1131.0
21	1669.0
22	2372.0
23	2360.0
24	2162.0
25	2806.0
26	3544.0
27	4747.0
28	6147.0
29	7225.0
30	7947.0
31	9327.0
32	11139.0
33	15472.0
34	25655.0
35	51785.0
36	140483.0
37	298869.0
38	359198.0
39	106265.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56808913769178	18.320466990997502	12.750118005847794	24.36132586546293
2	16.22289342663242	19.2883182814489	38.89025933430248	25.5985289576162
3	20.130796984099486	25.02664522494972	27.84200040673983	27.00055738421096
4	12.901090665320911	16.264885547931275	38.311973215429	32.522050571318815
5	13.906171147081642	37.702334234688884	34.667132408878985	13.724362209350497
6	31.561692640268753	35.999864420056795	18.77763382870227	13.660809110972183
7	28.273596559282332	30.20099726580448	21.937588032810346	19.587818142102844
8	26.320774613408854	34.46093791191823	19.822823376995096	19.395464097677817
9	26.51924858582587	15.394066871040875	19.67848722912257	38.40819731401068
10-11	26.452070607021533	24.60319328427348	27.71258746789392	21.23214864081107
12-13	26.8497246973931	22.49977403342799	27.562743384828604	23.087757884350307
14-15	23.72013475139911	24.662132898473217	24.821815942694876	26.795916407432795
16-17	23.59006274338483	27.160899497600987	25.912245881759226	23.33679187725496
18-19	23.467522954437605	26.57912972740899	26.956211444453647	22.99713587369975
20-21	24.5195811293539	25.299267296491646	26.68040681898212	23.500744755172327
22-23	24.405618536632673	25.97422161838222	25.91457515666023	23.705584688324876
24-25	24.165545461307353	25.500587985511736	26.30554909561332	24.028317457567596
26-27	23.97786028933026	26.57527939450518	26.273087502889396	23.173772813275164
28-29	23.613897883454904	26.586938038398923	26.627660746416637	23.171503331729536
30-31	23.818434748222266	25.64312598419637	26.73789364912355	23.800545618457818
32-33	23.70856512571515	25.939515310890133	26.875064435810636	23.47685512758408
34-35	24.403440641125652	25.655430270806196	26.197626426519165	23.743502661548984
36-37	23.492350887500425	25.723342033210177	26.794490831233688	23.98981624805571
38-39	23.676965720871028	26.00093776127385	26.184865135617603	24.13723138223752
40-41	24.114634725036343	25.890731981048937	26.526874957631268	23.467758336283453
42-43	23.959235630409076	25.85462440589622	26.712120470311756	23.474019493382944
44-45	24.270376011355904	25.538546284024754	26.39829002285776	23.792787681761578
46-47	23.919113108783634	25.53821726573285	26.221006657337913	24.321662968145606
48-49	23.467359133562724	25.65849713279349	26.72318381615983	24.15095991748396
50-51	23.96968658436462	25.787352274353548	26.252278496267785	23.990682645014047
52-53	24.123213980558393	26.064456053482548	25.72635341094347	24.08597655501559
54-55	24.161004948667927	25.378917695442254	26.21499401188584	24.245083344003977
56-57	24.197531212939122	25.71562951527726	25.900230645482498	24.18660862630112
58-59	24.444417234427224	25.816084667945432	26.189918908392716	23.549579189234628
60-61	24.132881085207146	26.073583086937212	25.894689851682017	23.898845976173625
62-63	23.888715229393732	25.656950731755085	26.397838629738708	24.05649540911248
64-65	24.13577201479328	25.391063398687884	26.058418008029342	24.41474657848949
66-67	23.678566317422774	25.71815001167494	25.9232617521448	24.680021918757486
68-69	24.065722377469626	25.69475305619789	26.342853430549173	23.89667113578331
70-71	24.329397121185874	25.57447293297078	26.072729224256758	24.023400721586587
72-73	23.828645782333933	25.695365048997086	26.48088134495304	23.995107823715944
74-75	24.11793007087818	25.407398898789573	26.313195317972628	24.161475712359618
76-77	24.033332015192517	24.97297811321323	26.174463469610266	24.81922640198399
78-79	24.019916631121433	24.98994846647025	26.180078284262077	24.81005661814624
80-81	23.713321403535534	25.691577534505317	26.114204980667044	24.48089608129211
82-83	23.612848373804425	25.774753776849636	26.500724275736033	24.111673573609906
84-85	23.932295087138016	25.268541573394458	26.68397233917047	24.11519100029706
86-87	23.533571100381884	26.08920595346595	26.249689295963485	24.12753365018868
88-89	24.106019749478396	25.258072655785117	25.67032042059911	24.965587174137372
90-91	23.91404231600672	25.51929942830457	25.83687661471946	24.729781640969247
92-93	24.038465159720708	25.59132627313333	26.336827655295526	24.03338091185044
94-95	23.944877337812493	25.43286721451007	26.026735611578527	24.595519836098916
96-97	24.117835918139843	25.759765522020444	26.205107974360324	23.91729058547939
98-99	22.783879921363635	26.02565285508764	26.568584620715107	24.62188260283362
100-101	23.517770548271088	25.64978410406721	26.177958923714257	24.654486423947443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	2.5
2	1.5
3	4.0
4	5.0
5	3.5
6	5.5
7	15.5
8	28.5
9	37.0
10	34.5
11	32.0
12	41.0
13	51.0
14	47.5
15	63.0
16	86.0
17	86.5
18	99.0
19	110.5
20	138.5
21	196.5
22	245.0
23	316.5
24	430.0
25	585.0
26	850.0
27	1229.0
28	1695.5
29	2382.0
30	3725.5
31	5745.5
32	8613.0
33	12419.5
34	16250.5
35	19715.0
36	23404.0
37	26464.0
38	27662.5
39	28275.0
40	28778.5
41	29020.0
42	29832.5
43	31326.5
44	32899.0
45	35277.0
46	37996.0
47	40218.0
48	41805.0
49	45323.0
50	49909.0
51	49707.5
52	47660.5
53	48725.0
54	50820.5
55	49121.0
56	41463.0
57	35239.5
58	33766.5
59	31969.5
60	26071.5
61	18887.0
62	14881.0
63	10565.0
64	6209.5
65	3796.0
66	2771.5
67	2415.0
68	1752.5
69	1004.5
70	530.0
71	326.0
72	265.0
73	215.5
74	151.0
75	106.5
76	76.5
77	45.5
78	28.5
79	18.0
80	10.5
81	7.0
82	6.0
83	3.5
84	1.5
85	1.0
86	1.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4663385129893118
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.415273833824182E-4
22-23	0.001176909229228023
24-25	2.824582150147255E-4
26-27	0.0028716585193163755
28-29	0.004048567748544399
30-31	6.119927991985719E-4
32-33	4.707636916912091E-5
34-35	0.0032482694726693427
36-37	0.0018830547667648364
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009556502941331545
46-47	0.08181872961593215
48-49	7.061455375368137E-4
50-51	0.0
52-53	4.707636916912091E-5
54-55	0.0
56-57	0.007955906389581434
58-59	0.03799062991948057
60-61	0.028010439655626943
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.8830547667648365E-4
78-79	0.0072497608520446215
80-81	0.0054137824544489045
82-83	8.002982758750556E-4
84-85	0.0023067420892869247
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06713090243516642
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1062104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.32547575322157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.67250245053849	35.3528028179675
2	15.776025800524208	15.563199562119093
3	5.636498832323343	8.340689594604802
4	2.5390324521692733	5.009559346244728
5	1.3841562864416368	3.4137083672773088
6	0.7613266444027186	2.2531679363260704
7	0.5140337995697317	1.774847320190921
8	0.3411051011287324	1.3460137116020388
9	0.22107894681306123	0.9814341807517871
>10	0.966366059281	8.318542917968696
>50	0.08505911604714211	2.898891949011083
>100	0.09058378887899449	9.506753489495974
>500	0.008981936381863414	3.0249145899422323
>1k	0.003248785499822937	2.215474216497734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2382	0.22427182272169205	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1840	0.17324103854236494	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1730	0.16288423732515836	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1716	0.16156609898842297	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1648	0.15516371278142255	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1397	0.13153137545852384	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1284	0.12089211602630251	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1276	0.12013889411959658	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1217	0.1145838825576403	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1196	0.11260667505253723	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1171	0.11025285659408118	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1167	0.1098762456407282	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1133	0.10667505253722799	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1124	0.10582767789218382	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1108	0.10432123407877195	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1096	0.10319140121871305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.415273833824183E-5	0.0
4	0.0	0.0	0.0	1.8830547667648365E-4	0.0
5	0.0	0.0	0.0	1.8830547667648365E-4	0.0
6	0.0	0.0	0.0	1.8830547667648365E-4	0.0
7	0.0	0.0	0.0	2.824582150147255E-4	0.0
8	0.0	0.0	0.0	2.824582150147255E-4	0.0
9	0.0	0.0	0.0	5.64916430029451E-4	0.0
10-11	0.0	0.0	0.0	6.590691683676928E-4	0.0
12-13	0.0	0.0	0.0	9.886037525515393E-4	0.0
14-15	0.0	0.0	0.0	0.001129832860058902	0.0
16-17	0.0	0.0	0.0	0.001176909229228023	0.0
18-19	0.0	0.0	0.0	0.0012239855983971438	0.0
20-21	0.0	0.0	0.0	0.0013181383367353856	0.0
22-23	0.0	0.0	0.0	0.0014593674442427483	0.0
24-25	0.0	0.0	0.0	0.0016476729209192321	0.0
26-27	0.0	0.0	0.0	0.002118436612610441	0.0
28-29	0.0	0.0	0.0	0.0025892003043016506	0.0
30-31	0.0	0.0	0.0	0.005837469776970993	0.0
32-33	0.0	0.0	0.0	0.013087230629015616	0.0
34-35	0.0	0.0	0.0	0.021843435294472104	0.0
36-37	0.0	0.0	0.0	0.03606049878354662	0.0
38-39	0.0	0.0	0.0	0.06115220355068807	0.0
40-41	0.0	0.0	0.0	0.09372905101571974	0.0
42-43	0.0	0.0	0.0	0.12272809442389823	0.0
44-45	0.0	0.0	0.0	0.1559640110572976	0.0
46-47	0.0	0.0	0.0	0.19423709919179288	0.0
48-49	0.0	0.0	0.0	0.23448739483139128	0.0
50-51	0.0	0.0	0.0	0.27949240375707085	0.0
52-53	0.0	0.0	0.0	0.32765152941708153	0.0
54-55	0.0	0.0	0.0	0.3767521824604747	0.0
56-57	0.0	0.0	0.0	0.43790438601116277	0.0
58-59	0.0	0.0	0.0	0.5056002048763586	0.0
60-61	0.0	0.0	0.0	0.5805457845935991	0.0
62-63	0.0	0.0	0.0	0.6609992995036267	0.0
64-65	0.0	0.0	0.0	0.7352858100524995	0.0
66-67	0.0	0.0	0.0	0.8262844316564103	0.0
68-69	0.0	0.0	0.0	0.929711214720969	0.0
70-71	0.0	0.0	0.0	1.0475904431204477	0.0
72-73	0.0	0.0	0.0	1.1748378689845815	0.0
74-75	0.0	0.0	0.0	1.300013934605274	0.0
76-77	0.0	0.0	0.0	1.4337108230455775	0.0
78-79	0.0	0.0	0.0	1.5899102159487206	0.0
80-81	0.0	0.0	0.0	1.7615977343085047	0.0
82-83	0.0	0.0	0.0	1.9539988551027019	0.0
84-85	0.0	0.0	0.0	2.1522374456738698	0.0
86-87	0.0	0.0	0.0	2.3646460233649433	0.0
88-89	0.0	0.0	0.0	2.6010635493322685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	150	0.0	53.821728	9
GGTATCA	860	0.0	44.383373	1
GTATCAA	1420	0.0	42.33611	1
AAACCGT	215	0.0	35.341217	6
ATCAACG	1730	0.0	34.31341	3
ACCGTGC	270	0.0	33.41872	8
TCAACGC	1795	0.0	33.070866	4
GTACATG	3905	0.0	29.690258	1
TATCACG	65	0.005850034	29.224468	2
AACCGTG	280	0.0	28.833069	7
TACATGG	3920	0.0	28.833069	2
TATACCG	135	4.23257E-7	28.14208	5
ATACCGT	190	3.965397E-10	27.494072	6
ACATGGG	4110	0.0	27.26906	3
CGCAGAG	2210	0.0	26.64584	8
ACGCAGA	2235	0.0	26.56027	7
GTCTAAT	565	0.0	26.178337	1
CAACGCA	2275	0.0	26.093275	5
GACCGCA	975	0.0	25.814947	7
AACGCAG	2320	0.0	25.587156	6
>>END_MODULE