FastQCFastQC Report
Fri 10 Feb 2017
SRR3224552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734830
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG17490.23801423458486995No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA11600.15785964100540262No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA8720.11866690254888886No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA8120.11050174870378184No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA7870.10709960126832056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT707.948802E-847.4748956
GTATCAA9300.042.933131
GGTATCA6000.035.6498341
ATCAACG11200.034.7584043
TCAACGC12150.031.649934
ACGCAGA13600.028.9736467
CGCAGAG13950.028.9312928
AATGCGG1354.241174E-728.133277
AAATGCG1405.8281694E-727.1285116
AACCGTG1056.927392E-527.128517
GTACATG24200.026.3199861
ACATGGG23700.026.2413963
CAACGCA14700.026.1596325
TACATGG24700.025.5634022
AACGCAG15350.025.361186
GACCGCA5350.024.8466727
TATCAAC15950.024.7048032
ATGCGGG1551.4143061E-624.5065048
GTATAGG1951.5563273E-824.3759541
AGACCGC5900.024.1397746
Produced by FastQC (version 0.11.5)