##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224552_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734830
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20196235864078	34.0	31.0	34.0	31.0	34.0
2	32.36206061265871	34.0	31.0	34.0	31.0	34.0
3	32.51258522379326	34.0	31.0	34.0	31.0	34.0
4	35.78774274321952	37.0	37.0	37.0	35.0	37.0
5	35.79583985411592	37.0	37.0	37.0	35.0	37.0
6	35.898225439897665	37.0	37.0	37.0	35.0	37.0
7	35.8629152320945	37.0	37.0	37.0	35.0	37.0
8	35.89625355524407	37.0	37.0	37.0	35.0	37.0
9	37.635693970033884	39.0	39.0	39.0	35.0	39.0
10-11	37.70906740334499	39.0	39.0	39.0	35.0	39.0
12-13	37.717963338459235	39.0	39.0	39.0	35.0	39.0
14-15	39.2222139814651	41.0	40.0	41.0	36.0	41.0
16-17	39.05947906318468	41.0	40.0	41.0	36.0	41.0
18-19	39.13582529292489	41.0	40.0	41.0	36.0	41.0
20-21	39.20327014411497	41.0	40.0	41.0	36.0	41.0
22-23	39.16193677449206	41.0	40.0	41.0	36.0	41.0
24-25	39.10783718683233	41.0	40.0	41.0	36.0	41.0
26-27	38.9423512921356	41.0	39.0	41.0	35.5	41.0
28-29	38.86313705210729	41.0	39.0	41.0	35.0	41.0
30-31	38.79912428725011	41.0	39.0	41.0	35.0	41.0
32-33	38.60708939482601	41.0	39.0	41.0	35.0	41.0
34-35	38.61131554237035	40.5	39.0	41.0	35.0	41.0
36-37	38.62967897336799	40.0	39.0	41.0	35.0	41.0
38-39	38.52707156757345	40.0	39.0	41.0	35.0	41.0
40-41	38.44418777132125	40.0	38.0	41.0	34.0	41.0
42-43	38.30390226310847	40.0	38.0	41.0	34.0	41.0
44-45	38.15141529333315	40.0	38.0	41.0	33.5	41.0
46-47	37.99402378781487	40.0	38.0	41.0	33.0	41.0
48-49	37.80856388552454	40.0	37.5	41.0	33.0	41.0
50-51	36.5508063089422	38.5	35.5	40.0	31.5	40.5
52-53	36.90302246778167	39.0	36.0	40.0	31.5	40.5
54-55	37.39240096348816	40.0	36.0	41.0	33.0	41.0
56-57	37.250674986051195	40.0	35.5	41.0	32.0	41.0
58-59	37.11549950328647	39.0	35.0	41.0	32.0	41.0
60-61	36.87062994161915	39.0	35.0	41.0	32.0	41.0
62-63	36.56427064763278	39.0	35.0	41.0	31.0	41.0
64-65	36.307381979505465	38.0	35.0	41.0	31.0	41.0
66-67	36.035090429078835	37.0	35.0	40.0	31.0	41.0
68-69	35.70435815086482	37.0	35.0	40.0	30.5	41.0
70-71	35.374873099900654	36.0	35.0	39.0	30.0	41.0
72-73	35.032985180245774	36.0	35.0	39.0	30.0	41.0
74-75	34.72736551311188	35.0	34.5	38.5	29.5	40.0
76-77	34.35885783106296	35.0	34.0	37.0	29.0	39.0
78-79	34.10516173808908	35.0	34.0	37.0	29.0	39.0
80-81	33.78627505681587	35.0	34.0	36.0	29.0	39.0
82-83	33.52470843596478	35.0	34.0	36.0	29.0	37.0
84-85	33.302859164704756	35.0	34.0	36.0	29.0	37.0
86-87	33.077149816964464	35.0	34.0	35.0	28.5	36.5
88-89	32.923362546439314	35.0	34.0	35.0	27.5	36.0
90-91	32.7596886354667	35.0	33.5	35.0	27.0	36.0
92-93	32.62515751942627	35.0	33.0	35.0	27.0	36.0
94-95	32.50426152987766	35.0	33.0	35.0	27.0	35.5
96-97	32.38038730046405	35.0	33.0	35.0	26.0	35.0
98-99	32.31858116843351	35.0	33.0	35.0	26.0	35.0
100-101	31.123998748009747	34.5	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	74.0
8	268.0
9	232.0
10	305.0
11	673.0
12	1212.0
13	1445.0
14	1527.0
15	1622.0
16	1565.0
17	1685.0
18	1905.0
19	2070.0
20	2471.0
21	2994.0
22	3309.0
23	3509.0
24	3557.0
25	3687.0
26	3974.0
27	4387.0
28	5036.0
29	5890.0
30	7239.0
31	9008.0
32	11581.0
33	16253.0
34	25964.0
35	46021.0
36	97386.0
37	183799.0
38	224501.0
39	59663.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63109291568656	17.841071082994215	12.572060150293964	24.955775851025265
2	16.209735585101313	18.88899473347577	39.216961746254235	25.684307935168675
3	20.547229355401054	23.78811941281577	27.66453147603048	28.0001197557527
4	12.818747193228367	15.728127594137419	37.64666657594273	33.80645863669148
5	14.091286419988297	37.970278840003814	34.227780575099004	13.71065416490889
6	32.4612495407101	35.87292298899065	18.084318822040473	13.581508648258781
7	29.044677000122476	30.174734292285287	21.005130438332674	19.77545826925956
8	26.424479131227628	34.96060313269736	19.642774519276568	18.972143216798443
9	26.462038838915124	15.414721772382729	19.093259665500863	39.029979723201286
10-11	26.27165466842671	24.872963814759878	27.4679177496836	21.387463767129812
12-13	26.680728876066574	22.19581399779541	27.583658805437995	23.539798320700026
14-15	23.494764843380942	24.32731198429521	24.63743256102691	27.540490611296935
16-17	23.856058496153665	26.996590493557893	25.701972229580246	23.445378780708197
18-19	23.65919842862772	26.57897799547945	26.41012081353634	23.351702762356485
20-21	24.523278728697896	25.31692357960444	26.434724599995235	23.725073091702434
22-23	24.358359808750574	25.84883614837491	25.749153308666823	24.04365073420769
24-25	23.906767641846628	25.638081834539715	26.206951934253613	24.24819858936004
26-27	23.882462610399685	26.47898153314372	26.143870010750785	23.49468584570581
28-29	23.699699250166706	26.56328674659445	26.24593443381462	23.49107956942422
30-31	23.832246914252273	25.97124504987548	26.21565532163902	23.980852714233226
32-33	23.67010056747819	25.968387246029696	26.46748227481186	23.894029911680253
34-35	24.25329668086496	25.673284977477785	26.14672781459657	23.926690527060682
36-37	23.722817505689676	25.789204316531094	26.51035030980149	23.97762786797774
38-39	23.74303633727161	26.118251788080098	26.05081815169234	24.087893722955947
40-41	24.28237218658051	25.801462202394728	26.311545465185592	23.604620145839174
42-43	24.05163550304877	25.889879552996746	26.30556835185413	23.75291659210035
44-45	24.137102695684305	25.65701052809227	26.171744481274885	24.034142294948545
46-47	23.97500102064423	25.617081501843963	26.035749765251825	24.37216771225998
48-49	23.668875794401426	25.9477702325708	26.33704394213628	24.046310030891497
50-51	23.916279955908166	26.085829375501817	25.97328633833676	24.024604330253254
52-53	24.009907053332064	26.111481567165196	25.661853762094633	24.216757617408106
54-55	24.088632744988637	25.546657049929916	26.012683205639398	24.352026999442046
56-57	23.96955758474749	26.04792945307078	25.850945116557572	24.131567845624158
58-59	24.084704204246098	26.103224146836588	26.195299851712523	23.61677179720479
60-61	24.098084424687702	26.145888059843692	25.924373293553682	23.83165422191492
62-63	23.69377702544961	25.855268486547594	26.363021936174164	24.08793255182863
64-65	24.32742088656067	25.38900632090596	25.873779302692085	24.40979348984128
66-67	23.65118458383892	25.942090036946574	25.69954519439316	24.707180184821347
68-69	23.775363009131365	25.94940326333982	26.4279493216118	23.847284405917016
70-71	24.03297361294449	25.60401725569179	26.23062477035505	24.13238436100867
72-73	23.79604806553897	25.870881700529374	26.44700134725038	23.886068886681272
74-75	23.95860267000531	25.713770531959774	26.22797109535539	24.09965570267953
76-77	23.83653362002096	25.441190479430613	26.025679408842862	24.696596491705563
78-79	23.86766861621258	25.115727350309854	26.391552481893992	24.625051551583567
80-81	23.81309340223523	25.874567235515794	25.845835418432618	24.466503943816363
82-83	23.563514961556216	26.028492107688894	26.164103974544183	24.24388895621071
84-85	23.894099846654683	25.484657641095726	26.432703322073493	24.188539190176098
86-87	23.563418342301297	26.355798513971436	25.989295637710242	24.091487506017035
88-89	23.97037752994068	25.584162324601557	25.832876721652926	24.61258342380484
90-91	23.695412544398025	25.52080072942041	26.012206904998436	24.77157982118313
92-93	23.565992222399895	25.709493367265353	26.50657829825021	24.21793611208454
94-95	23.838370779636104	25.552712872365035	26.34031000367432	24.268606344324535
96-97	24.061619694351073	25.741395969135716	26.28294979791244	23.914034538600763
98-99	23.330892839645955	25.878509683951535	26.309497240155466	24.48110023624705
100-101	23.77577152908917	25.571456384967384	26.016803656449504	24.635968429493943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.5
3	4.5
4	4.0
5	1.5
6	3.5
7	7.5
8	14.0
9	18.5
10	26.5
11	27.5
12	26.0
13	31.0
14	30.0
15	29.0
16	41.0
17	52.5
18	63.0
19	78.0
20	95.0
21	117.0
22	131.5
23	166.5
24	219.5
25	269.0
26	383.0
27	555.0
28	773.0
29	1131.5
30	1669.5
31	2649.0
32	4519.0
33	7161.0
34	10160.0
35	13270.5
36	16610.5
37	19472.0
38	21093.0
39	21682.5
40	21753.5
41	21892.0
42	22295.0
43	22948.0
44	23533.5
45	25087.5
46	27357.5
47	28343.0
48	29317.5
49	31003.5
50	32782.5
51	33044.5
52	32401.0
53	32683.5
54	33087.0
55	32174.0
56	27948.5
57	24224.5
58	22248.5
59	20091.0
60	17012.5
61	13336.5
62	10720.5
63	7981.0
64	5317.5
65	3613.0
66	2703.5
67	2166.0
68	1620.0
69	1107.5
70	765.0
71	501.5
72	319.5
73	227.5
74	175.5
75	129.0
76	88.5
77	59.5
78	41.0
79	22.5
80	17.5
81	13.5
82	6.0
83	7.0
84	8.0
85	6.0
86	4.5
87	3.0
88	5.0
89	6.0
90	6.0
91	6.0
92	3.5
93	2.5
94	4.5
95	5.0
96	2.5
97	2.0
98	2.5
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14533973844290518
2	0.0
3	1.3608589741845051E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0376957935849108
16-17	0.07614005960562308
18-19	0.02524393397112257
20-21	0.041846413456173535
22-23	0.06817903460664372
24-25	0.017418994869561666
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.022590258971462787
38-39	0.004558877563518093
40-41	0.006532123076085626
42-43	0.003470190384170488
44-45	0.011091000639603717
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.014765319869901884
60-61	0.015785964100540263
62-63	0.030143026278186793
64-65	0.04933113781418831
66-67	0.01639835063892329
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017555080766980116
80-81	0.06157886858184886
82-83	0.05184872691642965
84-85	0.02905433909883919
86-87	0.06212321217152267
88-89	0.03436168909815876
90-91	0.0
92-93	0.0037423621790073896
94-95	0.0
96-97	0.0
98-99	8.165153845107032E-4
100-101	0.04722180640420233
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	734830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.1373263627859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83992605438709	51.20719394130917
2	12.870632087586742	16.5097586139379
3	3.656580433275416	7.035698778622872
4	1.4674443197442586	3.7647182101861536
5	0.7074635010053365	2.268740872686919
6	0.3895086729243704	1.4989226925891583
7	0.22767255590938146	1.0221616315546733
8	0.14812599479748575	0.7600324216910935
9	0.09995197753878815	0.5769587343609989
>10	0.4901077670872753	5.749178501429619
>50	0.05107993422486527	2.33029521718262
>100	0.04851461097588415	5.822388225777096
>500	0.0025646490369766344	1.055201632644038
>1k	4.2744150616277237E-4	0.3987505260277354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1749	0.23801423458486995	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1160	0.15785964100540262	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	872	0.11866690254888886	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	812	0.11050174870378184	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	787	0.10709960126832056	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	1.3608589741845051E-4	0.0	0.0	0.0	0.0
16-17	2.7217179483690103E-4	0.0	0.0	0.0	0.0
18-19	2.7217179483690103E-4	0.0	0.0	0.0	0.0
20-21	2.7217179483690103E-4	0.0	0.0	2.7217179483690103E-4	0.0
22-23	2.7217179483690103E-4	0.0	0.0	3.402147435461263E-4	0.0
24-25	3.402147435461263E-4	0.0	0.0	5.443435896738022E-4	0.0
26-27	4.082576922553516E-4	0.0	0.0	6.804294870922527E-4	0.0
28-29	4.082576922553516E-4	0.0	0.0	0.0014969448716029557	0.0
30-31	5.443435896738021E-4	0.0	0.0	0.004558877563518093	0.0
32-33	5.443435896738021E-4	0.0	0.0	0.009730141665419214	0.0
34-35	6.123865383830273E-4	0.0	0.0	0.015105534613448008	0.0
36-37	6.804294870922527E-4	0.0	0.0	0.025039805124994895	0.0
38-39	6.804294870922527E-4	0.0	0.0	0.044976389096797895	0.0
40-41	6.804294870922527E-4	0.0	0.0	0.06953989358082822	0.0
42-43	6.804294870922527E-4	0.0	0.0	0.09396731216744009	0.0
44-45	6.804294870922527E-4	0.0	0.0	0.12186492113822245	0.0
46-47	6.804294870922527E-4	0.0	0.0	0.15350489228801217	0.0
48-49	6.804294870922527E-4	0.0	0.0	0.19405848971871045	0.0
50-51	6.804294870922527E-4	0.0	0.0	0.2321625409958766	0.0
52-53	6.804294870922527E-4	0.0	0.0	0.2729202672727025	0.0
54-55	6.804294870922527E-4	0.0	0.0	0.3168760121388621	0.0
56-57	6.804294870922527E-4	0.0	0.0	0.3706979845678592	0.0
58-59	6.804294870922527E-4	0.0	0.0	0.4300994787910129	0.0
60-61	6.804294870922527E-4	0.0	0.0	0.4981424275002382	0.0
62-63	6.804294870922527E-4	0.0	0.0	0.5699277383884709	0.0
64-65	6.804294870922527E-4	0.0	0.0	0.6463399697889307	0.0
66-67	6.804294870922527E-4	0.0	0.0	0.7419403127253923	0.0
68-69	8.165153845107032E-4	0.0	0.0	0.8488357851475852	0.0
70-71	8.165153845107032E-4	0.0	0.0	0.9648490126968142	0.0
72-73	8.165153845107032E-4	0.0	0.0	1.0910006396037177	0.0
74-75	8.165153845107032E-4	0.0	0.0	1.2142264197161248	0.0
76-77	8.165153845107032E-4	0.0	0.0	1.3431678075201066	0.0
78-79	8.165153845107032E-4	0.0	0.0	1.496400528013282	0.0
80-81	8.165153845107032E-4	0.0	0.0	1.6639222677353946	0.0
82-83	8.165153845107032E-4	0.0	0.0	1.847093885660629	0.0
84-85	8.165153845107032E-4	0.0	0.0	2.0465958112760774	0.0
86-87	8.165153845107032E-4	0.0	0.0	2.2598424125307894	6.804294870922526E-5
88-89	8.165153845107032E-4	0.0	0.0	2.4971762176285672	1.3608589741845051E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	70	7.948802E-8	47.474895	6
GTATCAA	930	0.0	42.93313	1
GGTATCA	600	0.0	35.649834	1
ATCAACG	1120	0.0	34.758404	3
TCAACGC	1215	0.0	31.64993	4
ACGCAGA	1360	0.0	28.973646	7
CGCAGAG	1395	0.0	28.931292	8
AATGCGG	135	4.241174E-7	28.13327	7
AAATGCG	140	5.8281694E-7	27.128511	6
AACCGTG	105	6.927392E-5	27.12851	7
GTACATG	2420	0.0	26.319986	1
ACATGGG	2370	0.0	26.241396	3
CAACGCA	1470	0.0	26.159632	5
TACATGG	2470	0.0	25.563402	2
AACGCAG	1535	0.0	25.36118	6
GACCGCA	535	0.0	24.846672	7
TATCAAC	1595	0.0	24.704803	2
ATGCGGG	155	1.4143061E-6	24.506504	8
GTATAGG	195	1.5563273E-8	24.375954	1
AGACCGC	590	0.0	24.139774	6
>>END_MODULE