FastQCFastQC Report
Fri 10 Feb 2017
SRR3224552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734830
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG11170.15200794741640924No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA9590.13050637562429407No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA9390.12778465767592503No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA8600.11703387177986746No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT8070.10982131921668957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.044.219491
GTATCAA9900.037.5900881
AAACCGT651.3299417E-436.5280726
ATCAACG12950.028.6019023
CGCGGTC1004.9603175E-528.4918969
TCAACGC13100.028.27444
GTACCGT856.415624E-427.933236
CAACGCA14050.026.362615
AACCGTG1457.900653E-726.1994467
GTACATG23750.026.1152231
ACCGTGC1858.983989E-925.6683738
TACCGTG1301.0036774E-525.569657
AACGCAG14650.025.2829116
CCGTGCG1151.2836144E-424.7755629
TACATGG25300.024.5878682
ACATGGG25000.024.5030293
ACGCAGA15200.024.3680697
CGCAGAG15200.024.3680698
ATAGCGT1401.7822662E-523.7432485
TAACCCG1401.7822662E-523.7432484
Produced by FastQC (version 0.11.5)