##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734830
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.151986173672825	34.0	33.0	34.0	31.0	34.0
2	33.28523740184804	34.0	34.0	34.0	31.0	34.0
3	33.39929099247445	34.0	34.0	34.0	31.0	34.0
4	36.61260155410095	37.0	37.0	37.0	35.0	37.0
5	36.6441380999687	37.0	37.0	37.0	35.0	37.0
6	36.68322469142523	37.0	37.0	37.0	36.0	37.0
7	36.66474286569683	37.0	37.0	37.0	35.0	37.0
8	36.64407413959692	37.0	37.0	37.0	35.0	37.0
9	38.48504824245064	39.0	39.0	39.0	37.0	39.0
10-11	38.507823578242586	39.0	39.0	39.0	37.0	39.0
12-13	38.503427323326484	39.0	39.0	39.0	37.0	39.0
14-15	40.11751901800417	41.0	40.0	41.0	38.0	41.0
16-17	40.125854279221045	41.0	40.0	41.0	38.0	41.0
18-19	40.10975327626798	41.0	40.0	41.0	38.0	41.0
20-21	40.11020984445382	41.0	40.0	41.0	38.0	41.0
22-23	40.03183117183566	41.0	40.0	41.0	38.0	41.0
24-25	40.00948926962699	41.0	40.0	41.0	38.0	41.0
26-27	39.95511070587755	41.0	40.0	41.0	38.0	41.0
28-29	39.91651062150429	41.0	40.0	41.0	38.0	41.0
30-31	39.868461412843786	41.0	40.0	41.0	38.0	41.0
32-33	39.83014710885511	41.0	40.0	41.0	38.0	41.0
34-35	39.75514540778139	41.0	40.0	41.0	37.5	41.0
36-37	39.66421757413279	41.0	40.0	41.0	37.0	41.0
38-39	39.57207789556768	41.0	40.0	41.0	37.0	41.0
40-41	39.44900861423731	41.0	39.5	41.0	36.5	41.0
42-43	39.37101982771526	41.0	39.0	41.0	36.0	41.0
44-45	39.14867452335915	41.0	39.0	41.0	35.0	41.0
46-47	38.940075935930764	41.0	39.0	41.0	35.0	41.0
48-49	38.865117782344214	40.0	39.0	41.0	35.0	41.0
50-51	38.741587169821585	40.0	38.0	41.0	35.0	41.0
52-53	38.52508743518909	40.0	37.5	41.0	35.0	41.0
54-55	38.35533184546085	40.0	37.0	41.0	35.0	41.0
56-57	38.15127716614727	40.0	36.5	41.0	34.5	41.0
58-59	37.893137868622674	40.0	36.0	41.0	34.0	41.0
60-61	37.64448103643019	39.0	35.0	41.0	34.0	41.0
62-63	37.40618986704408	39.0	35.0	41.0	34.0	41.0
64-65	37.11984336513207	38.5	35.0	41.0	33.5	41.0
66-67	36.81296354258808	37.5	35.0	40.0	33.0	41.0
68-69	36.5191779050937	37.0	35.0	40.0	33.0	41.0
70-71	36.19277247798811	36.5	35.0	39.0	33.0	41.0
72-73	35.85836860226175	36.0	35.0	39.0	33.0	41.0
74-75	35.51569682783773	35.5	35.0	39.0	32.0	40.5
76-77	33.66612141583768	34.5	32.5	36.5	29.5	39.0
78-79	34.93615734251459	35.0	34.5	37.0	32.0	39.0
80-81	34.84027870391791	35.0	35.0	36.5	33.0	39.0
82-83	34.62002503980513	35.0	35.0	36.0	33.0	37.5
84-85	34.41562538274158	35.0	35.0	36.0	32.0	37.0
86-87	34.21299824449193	35.0	35.0	35.5	32.0	37.0
88-89	34.054503082345576	35.0	35.0	35.0	32.0	36.0
90-91	33.909749193691056	35.0	34.0	35.0	32.0	36.0
92-93	33.81601731012615	35.0	34.0	35.0	32.0	36.0
94-95	33.716943374658086	35.0	34.0	35.0	31.0	36.0
96-97	33.66808445490794	35.0	34.0	35.0	31.0	35.0
98-99	33.624755385599386	35.0	34.0	35.0	31.0	35.0
100-101	32.79197501462924	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	8.0
15	34.0
16	83.0
17	156.0
18	248.0
19	444.0
20	708.0
21	1047.0
22	1419.0
23	1543.0
24	1503.0
25	1817.0
26	2262.0
27	2982.0
28	3888.0
29	4564.0
30	5294.0
31	6048.0
32	7892.0
33	10428.0
34	17653.0
35	34066.0
36	87872.0
37	198512.0
38	267743.0
39	76593.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13625859735003	17.73727318720687	12.588161295174544	24.538306920268553
2	16.191363988949824	18.996366506538926	39.47293931929834	25.339330185212905
3	20.304151980730236	24.113604507164922	27.85188410924976	27.730359402855083
4	12.736006967597948	15.908441408216866	37.91788576949771	33.437665854687474
5	13.991807628975408	37.30318577085857	34.93352203911109	13.771484561054939
6	32.53351115223929	35.635997441585126	18.251432304070327	13.579059102105248
7	29.12442333600969	30.130642461521713	21.21361403317774	19.531320169290854
8	26.27995590816924	34.97475606602888	19.60045180517943	19.14483622062246
9	26.471837023529254	15.519780085189772	19.143203189853438	38.86517970142754
10-11	26.304179197909722	24.79559898207749	27.691234707347277	21.208987112665515
12-13	26.758161751697674	22.47860049263095	27.46261039968428	23.3006273559871
14-15	23.586407740565846	24.468312398786114	24.86302954424833	27.082250316399715
16-17	23.75100363349346	27.181048677925506	25.79106732169345	23.27688036688758
18-19	23.462501530966346	26.639698977994907	26.827089258740116	23.070710232298627
20-21	24.307383284659416	25.571771122802527	26.799075150172424	23.321770442365633
22-23	24.191960327618318	25.90939123062362	26.132235375130048	23.76641306662802
24-25	24.05937171691879	25.54155530273751	26.397674013510645	24.001398966833055
26-27	23.867507148038218	26.60077270939567	26.22510094350022	23.30661919906589
28-29	23.70097128602516	26.465060513147133	26.52432842178609	23.309639779041618
30-31	23.79585685075749	25.81109171880475	26.56147191007413	23.831579520363622
32-33	23.612568366245753	25.990604616856437	26.79909203365681	23.597734983240997
34-35	24.283088698104184	25.79451729480683	26.277164649228396	23.645229357860593
36-37	23.651538678232324	25.76253471943431	26.687320278871397	23.89860632346197
38-39	23.767810241824638	26.058816324864253	26.20415606330716	23.969217370003946
40-41	23.989358082821877	25.99839418641046	26.444211586353305	23.568036144414354
42-43	23.886953445014495	25.938652477443764	26.57567056325953	23.598723514282216
44-45	24.14825118100913	25.724495170485863	26.40036855923011	23.726885089274898
46-47	23.842241213168723	25.692190035677825	26.28023745266167	24.18533129849178
48-49	23.62814275507774	25.738305038614634	26.71255060728745	23.921001599020176
50-51	24.000040825769226	25.809643046691072	26.301593565858774	23.888722561680932
52-53	24.02514867384293	26.156117741518447	25.947702189622092	23.871031395016534
54-55	24.082644965502226	25.607555489024676	26.276825932528613	24.03297361294449
56-57	24.016462271918734	25.833082688347798	26.081731587120338	24.068723452613128
58-59	24.159337816864976	25.89974678029787	26.34260897976965	23.5983064230675
60-61	24.104279312391437	26.23733331155289	26.04178577846023	23.616601597595437
62-63	23.77420627900331	25.79304056720602	26.463807955581565	23.96894519820911
64-65	24.000176911666642	25.5199842140359	26.196331124205596	24.283507750091857
66-67	23.744267381571248	25.84053454540506	26.054121361403315	24.361076711620374
68-69	24.07420763986228	25.674101492862295	26.363580692132878	23.88811017514255
70-71	24.14197841677667	25.572445327490712	26.38344923315597	23.90212702257665
72-73	23.794891335410913	25.760107780030754	26.438904236353988	24.00609664820435
74-75	23.95860267000531	25.614223697998177	26.371949974824112	24.055223657172405
76-77	23.848353731516948	25.29413325694363	26.357102371505732	24.500410640033696
78-79	23.996793604629403	25.224573563705672	26.271143190186468	24.507489641478454
80-81	23.653444548555612	25.89523694816829	26.116183978720493	24.335134524555606
82-83	23.557954013478057	25.861090546852033	26.484641220210552	24.09631421945935
84-85	23.905614852092828	25.486752520008327	26.492238224651242	24.115394403247603
86-87	23.51659567519018	26.21953376971544	26.2877808472708	23.97608970782358
88-89	23.992488058462502	25.485146224296777	25.834750894764774	24.687614822475947
90-91	23.874501585400704	25.573125756977806	25.952601281929155	24.599771375692335
92-93	23.8759304873236	25.68709769606576	26.400936270974242	24.036035545636405
94-95	23.838710994379653	25.510798415960156	26.30050487867942	24.34998571098077
96-97	24.084618211014792	25.80283875182015	26.194085706898196	23.918457330266865
98-99	23.04492195473783	26.012138862049728	26.44815807737844	24.494781105834
100-101	23.52722491664244	25.725195465566536	26.141285364407192	24.606294253383837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	3.0
5	1.0
6	3.0
7	6.5
8	15.0
9	26.0
10	26.0
11	32.0
12	36.0
13	29.0
14	37.5
15	52.0
16	61.5
17	62.5
18	58.0
19	73.0
20	92.0
21	114.0
22	142.0
23	174.0
24	232.0
25	342.5
26	461.0
27	598.5
28	780.5
29	1129.0
30	1763.0
31	2942.5
32	4921.5
33	7471.5
34	10499.0
35	13624.0
36	16847.0
37	19625.5
38	21138.5
39	21753.5
40	21818.5
41	21889.5
42	22404.5
43	22994.5
44	23745.5
45	25274.0
46	26835.5
47	28297.0
48	29678.0
49	31573.5
50	33688.5
51	33548.5
52	32017.5
53	32186.0
54	33194.0
55	32235.5
56	28155.0
57	24216.0
58	22266.0
59	20359.0
60	16933.0
61	12955.0
62	10357.5
63	7454.5
64	4645.5
65	3061.0
66	2251.0
67	1802.5
68	1325.5
69	849.5
70	534.5
71	345.5
72	236.5
73	174.0
74	121.5
75	88.5
76	60.0
77	33.0
78	18.5
79	11.5
80	8.0
81	5.0
82	2.5
83	1.0
84	1.0
85	0.5
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4763006409645768
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0010886871793476041
22-23	0.0015649878203121811
24-25	2.7217179483690103E-4
26-27	0.0017691166664398567
28-29	0.004218662819971967
30-31	3.402147435461263E-4
32-33	1.3608589741845051E-4
34-35	0.00387844807642584
36-37	0.0014969448716029559
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.01047861410122069
46-47	0.08444129934814856
48-49	6.804294870922526E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.008029067947688581
58-59	0.039464910251350654
60-61	0.030755412816569818
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.041288461276758E-4
78-79	0.006600166024794851
80-81	0.005239307050610345
82-83	8.165153845107032E-4
84-85	0.0014969448716029559
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06715839037600534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	734830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.501864880073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.67113436930076	49.17092610312103
2	13.37851638128767	16.723644463181703
3	4.035297774882174	7.566409088296347
4	1.5918886217518093	3.979840301634289
5	0.7461117593823637	2.331668818517502
6	0.42443267092942244	1.591670006947155
7	0.23719475362977976	1.0377580109141489
8	0.1639135460727453	0.8195921846921876
9	0.12022522441423018	0.6762870658363201
>10	0.5178765491636486	5.9379912838628135
>50	0.05888546620818729	2.6291980379561113
>100	0.050815326936266274	5.950532379891197
>500	0.003489464509070384	1.4322225614980535
>1k	2.18091531816899E-4	0.15225969365121592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1117	0.15200794741640924	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	959	0.13050637562429407	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	939	0.12778465767592503	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	860	0.11703387177986746	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	807	0.10982131921668957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	1.3608589741845051E-4	0.0	0.0	0.0	0.0
14-15	1.3608589741845051E-4	0.0	0.0	0.0	0.0
16-17	1.3608589741845051E-4	0.0	0.0	0.0	0.0
18-19	1.3608589741845051E-4	0.0	0.0	0.0	0.0
20-21	1.3608589741845051E-4	0.0	0.0	1.3608589741845051E-4	1.3608589741845051E-4
22-23	1.3608589741845051E-4	0.0	0.0	2.0412884612767577E-4	1.3608589741845051E-4
24-25	1.3608589741845051E-4	0.0	0.0	4.0825769225535154E-4	1.3608589741845051E-4
26-27	2.0412884612767577E-4	0.0	0.0	5.443435896738021E-4	1.3608589741845051E-4
28-29	2.7217179483690103E-4	0.0	0.0	0.0013608589741845051	1.3608589741845051E-4
30-31	2.7217179483690103E-4	0.0	0.0	0.004490834614808868	1.3608589741845051E-4
32-33	2.7217179483690103E-4	0.0	0.0	0.009798184614128437	1.3608589741845051E-4
34-35	2.7217179483690103E-4	0.0	0.0	0.01564987820312181	2.7217179483690103E-4
36-37	2.7217179483690103E-4	0.0	0.0	0.025652191663377927	2.7217179483690103E-4
38-39	2.7217179483690103E-4	0.0	0.0	0.04531660384034403	2.7217179483690103E-4
40-41	2.7217179483690103E-4	0.0	0.0	0.06988010832437434	2.7217179483690103E-4
42-43	2.7217179483690103E-4	0.0	0.0	0.09437556985969545	2.7217179483690103E-4
44-45	2.7217179483690103E-4	0.0	0.0	0.1230896942149885	2.7217179483690103E-4
46-47	2.7217179483690103E-4	0.0	0.0	0.15493379421090592	2.7217179483690103E-4
48-49	2.7217179483690103E-4	0.0	0.0	0.19705237946191637	2.7217179483690103E-4
50-51	2.7217179483690103E-4	0.0	0.0	0.23576881727746551	2.7217179483690103E-4
52-53	2.7217179483690103E-4	0.0	0.0	0.27700284419525606	2.7217179483690103E-4
54-55	2.7217179483690103E-4	0.0	0.0	0.32163901854850785	4.082576922553516E-4
56-57	2.7217179483690103E-4	0.0	0.0	0.37552903392621423	5.443435896738022E-4
58-59	2.7217179483690103E-4	0.0	0.0	0.43547487173904165	6.804294870922527E-4
60-61	4.082576922553516E-4	0.0	0.0	0.5042662928840684	6.804294870922527E-4
62-63	4.082576922553516E-4	0.0	0.0	0.5760516037723011	6.804294870922527E-4
64-65	4.082576922553516E-4	0.0	0.0	0.653688608249527	6.804294870922527E-4
66-67	4.082576922553516E-4	0.0	0.0	0.7504456813140454	6.804294870922527E-4
68-69	4.082576922553516E-4	0.0	0.0	0.8580215832233307	6.804294870922527E-4
70-71	4.082576922553516E-4	0.0	0.0	0.9747832832083612	6.804294870922527E-4
72-73	4.082576922553516E-4	0.0	0.0	1.1015472966536477	6.804294870922527E-4
74-75	4.082576922553516E-4	0.0	0.0	1.2264061075350763	7.484724358014779E-4
76-77	4.082576922553516E-4	0.0	0.0	1.357184654954207	8.165153845107032E-4
78-79	4.082576922553516E-4	0.0	0.0	1.5106895472422193	8.165153845107032E-4
80-81	4.082576922553516E-4	0.0	0.0	1.6790958452975517	8.165153845107032E-4
82-83	4.082576922553516E-4	0.0	0.0	1.8649891811711552	8.165153845107032E-4
84-85	4.082576922553516E-4	0.0	0.0	2.0665323952478802	8.165153845107032E-4
86-87	4.082576922553516E-4	0.0	0.0	2.2833172298354723	8.165153845107032E-4
88-89	4.082576922553516E-4	0.0	0.0	2.5220799368561435	8.165153845107032E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	615	0.0	44.21949	1
GTATCAA	990	0.0	37.590088	1
AAACCGT	65	1.3299417E-4	36.528072	6
ATCAACG	1295	0.0	28.601902	3
CGCGGTC	100	4.9603175E-5	28.491896	9
TCAACGC	1310	0.0	28.2744	4
GTACCGT	85	6.415624E-4	27.93323	6
CAACGCA	1405	0.0	26.36261	5
AACCGTG	145	7.900653E-7	26.199446	7
GTACATG	2375	0.0	26.115223	1
ACCGTGC	185	8.983989E-9	25.668373	8
TACCGTG	130	1.0036774E-5	25.56965	7
AACGCAG	1465	0.0	25.282911	6
CCGTGCG	115	1.2836144E-4	24.775562	9
TACATGG	2530	0.0	24.587868	2
ACATGGG	2500	0.0	24.503029	3
ACGCAGA	1520	0.0	24.368069	7
CGCAGAG	1520	0.0	24.368069	8
ATAGCGT	140	1.7822662E-5	23.743248	5
TAACCCG	140	1.7822662E-5	23.743248	4
>>END_MODULE