FastQCFastQC Report
Fri 10 Feb 2017
SRR3224551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57904
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC4270.7374274661508704No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT2270.39202818458137606No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA1970.3402182923459519No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA1520.2625034539928157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.2504144791378834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.19860458690245925No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA1100.1899696048632219No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC1070.18478861563967947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.17442663719259463No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG880.1519756838905775No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC850.1467946946670351No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG820.1416137054434927No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA800.13815971262779772No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA800.13815971262779772No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC760.13125172699640786No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG740.1277977341807129No Hit
GCACTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCT690.11916275214147555No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA690.11916275214147555No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA670.11570875932578059No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA660.11398176291793312No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA650.11225476651008566No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC630.10880077369439071No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG610.10534678087869577No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG600.10361978447084831No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC600.10361978447084831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1200.079.145791
CCGTGCG408.926145E-871.231229
AAACCGT353.3815013E-667.839266
AACCGTG407.4673644E-659.359357
TCTACAG250.005002507556.9849743
ACATGAG250.005002507556.9849743
ACCGTGC504.179201E-756.9849748
GTATCAA1750.054.2714042
CATGGGA804.116373E-947.4874764
ATGGGAG554.9125403E-543.1704335
TCAACGC3100.038.2963523
TACAAAA756.8215286E-637.989982
ATCAACG3150.037.6884772
CAAAACC905.5859346E-736.9347044
TATCAAC3250.036.5288281
ACGCAGA3400.036.3139536
CAGAGTA3400.036.3139539
CGCAGAG3450.035.7876667
CGTGCGC401.0648726E-535.64639310-11
AACGCAG3500.035.2764135
Produced by FastQC (version 0.11.5)