##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224551_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57904
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10857626416137	34.0	31.0	34.0	31.0	34.0
2	32.25616537717602	34.0	31.0	34.0	31.0	34.0
3	32.369369991710414	34.0	31.0	34.0	30.0	34.0
4	35.731624758220505	37.0	35.0	37.0	35.0	37.0
5	35.7753523072672	37.0	37.0	37.0	35.0	37.0
6	35.86772934512296	37.0	37.0	37.0	35.0	37.0
7	35.80569563415308	37.0	37.0	37.0	35.0	37.0
8	35.856175739154466	37.0	37.0	37.0	35.0	37.0
9	37.57248203923736	39.0	38.0	39.0	35.0	39.0
10-11	37.6161318734457	39.0	38.5	39.0	35.0	39.0
12-13	37.61214251174357	39.0	38.5	39.0	35.0	39.0
14-15	39.03492850234871	41.0	39.0	41.0	36.0	41.0
16-17	38.8845071152252	41.0	39.0	41.0	35.5	41.0
18-19	39.01077645758497	41.0	39.5	41.0	36.0	41.0
20-21	39.0708845675601	41.0	40.0	41.0	36.0	41.0
22-23	38.9998013954131	41.0	39.5	41.0	35.5	41.0
24-25	38.92920178226029	41.0	39.0	41.0	35.0	41.0
26-27	38.72462178778669	41.0	39.0	41.0	35.0	41.0
28-29	38.643919245647965	41.0	39.0	41.0	35.0	41.0
30-31	38.494672216081796	40.5	38.5	41.0	34.5	41.0
32-33	38.21270551257253	40.0	38.0	41.0	34.0	41.0
34-35	38.1631148107212	40.0	38.0	41.0	34.0	41.0
36-37	38.09022692732799	40.0	38.0	41.0	34.0	41.0
38-39	37.938026733904394	40.0	38.0	41.0	33.0	41.0
40-41	37.765577507598785	40.0	38.0	41.0	33.0	41.0
42-43	37.55674046697983	40.0	37.0	41.0	32.5	41.0
44-45	37.259861149488806	40.0	37.0	41.0	31.5	41.0
46-47	36.98567456479691	40.0	36.0	41.0	30.5	41.0
48-49	36.654712973197014	40.0	36.0	41.0	29.5	41.0
50-51	35.35562310030395	38.5	34.0	40.0	27.5	40.5
52-53	35.648141751865154	38.5	35.0	40.0	28.0	40.5
54-55	36.06513366952197	39.0	35.0	41.0	28.5	41.0
56-57	35.896604725062176	39.0	35.0	41.0	28.0	41.0
58-59	35.71245509809339	38.0	35.0	41.0	28.0	41.0
60-61	35.45896656534954	37.0	35.0	41.0	27.5	41.0
62-63	35.18684374136502	37.0	35.0	40.0	27.0	41.0
64-65	34.94748203923736	36.0	35.0	40.0	27.0	41.0
66-67	34.707015059408675	36.0	34.5	40.0	26.5	41.0
68-69	34.45458862945566	35.0	34.0	39.5	26.0	41.0
70-71	34.1688829787234	35.0	34.0	39.0	26.0	41.0
72-73	33.85336936999171	35.0	34.0	39.0	25.5	41.0
74-75	33.592368402873724	35.0	34.0	37.5	25.0	40.0
76-77	33.27930712904117	35.0	34.0	37.0	24.0	39.5
78-79	33.08470053882288	35.0	34.0	37.0	24.0	39.0
80-81	32.81645482177397	35.0	33.0	36.0	24.0	39.0
82-83	32.55871787786681	35.0	33.0	36.0	24.0	37.5
84-85	32.33825815142305	35.0	33.0	35.5	24.0	37.0
86-87	32.14244266371926	35.0	33.0	35.0	23.5	37.0
88-89	31.968957239568944	35.0	33.0	35.0	23.0	36.0
90-91	31.78306334622824	35.0	33.0	35.0	22.5	36.0
92-93	31.63496476927328	35.0	33.0	35.0	21.0	36.0
94-95	31.497547665100853	35.0	32.0	35.0	20.0	36.0
96-97	31.35450918762089	35.0	32.0	35.0	20.0	35.0
98-99	31.283339665653497	35.0	32.0	35.0	20.0	35.0
100-101	30.124706410610663	34.0	29.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	23.0
9	14.0
10	27.0
11	60.0
12	136.0
13	126.0
14	166.0
15	141.0
16	151.0
17	223.0
18	302.0
19	359.0
20	409.0
21	551.0
22	644.0
23	588.0
24	447.0
25	417.0
26	421.0
27	422.0
28	438.0
29	519.0
30	634.0
31	749.0
32	997.0
33	1381.0
34	2291.0
35	4092.0
36	8377.0
37	13831.0
38	14038.0
39	4919.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15850283658503	19.684862321848623	11.629998616299986	22.526636225266362
2	16.76395413097541	20.230035921525282	37.76941143962421	25.2365985078751
3	18.168002210555404	26.887607073777286	31.046214423873998	23.898176291793312
4	11.30491848576955	16.725960210002764	40.57232660956065	31.396794694667037
5	11.58469190384084	41.21995026250345	34.9388643271622	12.256493506493507
6	27.54559270516717	40.17857142857143	18.589389334070187	13.686446532191212
7	23.798010500138158	33.726512848853275	24.02942801878972	18.446048632218844
8	26.716634429400386	34.491572257529704	20.508082343188725	18.283710969881184
9	25.790964354794145	12.51381597126278	21.786059684995855	39.90915998894722
10-11	25.507736943907155	24.816938380768168	29.271725614810723	20.403599060513955
12-13	25.621718706825092	23.587316938380766	29.642166344294	21.14879801050014
14-15	23.0774545705797	25.923270918261593	26.09600635666413	24.903268154494576
16-17	22.131225433026653	27.75517256274027	28.119735648568096	21.99386635566498
18-19	21.67105660866186	26.655727794809792	29.357053413359818	22.31616218316853
20-21	23.52184201889693	24.519371944794706	29.65816247214689	22.30062356416147
22-23	23.100910457301797	26.540607777758584	28.47036262806005	21.888119136879567
24-25	22.25465473764206	26.07343949704653	28.716017824449896	22.955887940861516
26-27	22.241123238463665	26.690729483282677	29.235458690245924	21.832688588007738
28-29	21.74547526941144	27.34785161646864	29.322672008842222	21.5840011052777
30-31	21.69970986460348	25.833275766786407	29.919349267753525	22.54766510085659
32-33	22.213491295938105	26.014610389610386	29.81918347609837	21.952714838353135
34-35	22.451816800221057	26.13722713456756	29.4418347609837	21.969121304227688
36-37	22.440995880722298	25.56283841550299	29.809925991174207	22.1862397126005
38-39	22.500755580501703	25.848624843486895	28.69306161219291	22.957557963818488
40-41	23.074598253991553	25.778234476326993	29.320334694793925	21.82683257488753
42-43	22.788777880439007	26.370599359278803	28.818637906171475	22.02198485411072
44-45	22.28871445472052	26.324879320915695	28.203069005120767	23.183337219243025
46-47	23.08476098369715	26.00079441834761	27.53091323570047	23.383531362254768
48-49	22.472540757115226	26.2624343741365	28.17162890301188	23.09339596573639
50-51	22.57529704338215	27.00245233489914	27.23127935893893	23.190971262779772
52-53	23.739292622271346	27.201920420005525	26.18385603757944	22.874930920143687
54-55	22.661646863774525	26.34360320530533	27.113843603205307	23.880906327714836
56-57	23.168520309477756	27.367712075158884	26.392822602928984	23.070945012434375
58-59	23.874577939741453	26.879733335636136	26.552447733611974	22.69324099101044
60-61	23.324035613433622	27.061546300054406	26.96482698468899	22.649591101822985
62-63	23.548969316994395	26.503277286976346	27.31590628427334	22.631847111755917
64-65	23.797061643303188	26.376976826540222	26.693960044567667	23.132001485588923
66-67	22.821046450376077	26.24934154281915	26.45400298788439	24.475609018920387
68-69	22.80239707101409	26.740812379110253	27.451471400939486	23.00531914893617
70-71	23.69439071566731	25.922216081790552	27.01799530256977	23.36539789997237
72-73	23.63135534678088	26.429089527493783	26.998998342083446	22.94055678364189
74-75	22.958690245924288	26.49298839458414	27.207101409229068	23.341219950262506
76-77	23.412890301188174	25.674392097264437	26.455857971815416	24.45685962973197
78-79	23.233762532708102	25.33830755546344	27.03610628945482	24.391823622373636
80-81	22.982439471024698	26.467768266664365	27.03181280286082	23.51797945945012
82-83	23.228013198127385	26.799626859225732	26.716706687166376	23.255653255480503
84-85	22.654887127139574	26.06698101801475	27.611102475085065	23.66702937976061
86-87	22.431052799778886	27.507190547345328	27.01400105374988	23.047755599125903
88-89	23.242216176534093	26.644211253616618	26.202012350477176	23.911560219372113
90-91	22.57097955236253	26.692456479690524	26.876381597126276	23.860182370820667
92-93	22.589503134552615	26.779268777092724	27.080634854843446	23.550593233511215
94-95	23.19010776457585	26.272796352583587	27.05944321635811	23.477652666482456
96-97	23.207377728654325	26.692456479690524	27.238187344570324	22.86197844708483
98-99	21.736840287372203	27.826229621442387	26.986909367228517	23.450020723956893
100-101	22.991483995785185	26.54471334058835	26.76927328945777	23.694529374168695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.5
4	3.0
5	2.5
6	3.0
7	7.0
8	12.0
9	16.0
10	21.0
11	21.5
12	25.0
13	31.0
14	29.5
15	33.5
16	35.0
17	34.5
18	45.0
19	49.5
20	69.5
21	89.5
22	105.5
23	156.5
24	229.0
25	312.5
26	427.5
27	541.5
28	606.5
29	698.0
30	808.0
31	897.0
32	1007.0
33	1097.0
34	1164.5
35	1260.5
36	1331.0
37	1359.0
38	1391.0
39	1425.0
40	1417.5
41	1409.5
42	1404.0
43	1441.0
44	1548.0
45	1747.5
46	1918.5
47	1832.0
48	1884.5
49	2210.0
50	2530.5
51	2458.0
52	2214.5
53	2458.0
54	2632.5
55	2436.5
56	1975.5
57	1602.0
58	1492.5
59	1402.0
60	1203.5
61	909.0
62	725.0
63	517.5
64	322.5
65	205.5
66	161.5
67	149.5
68	118.5
69	79.0
70	46.5
71	29.5
72	26.0
73	19.5
74	7.5
75	5.0
76	4.0
77	2.0
78	1.5
79	2.5
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1519756838905775
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.02072395689416966
16-17	0.045765404807957995
18-19	0.011225476651008565
20-21	0.018996960486322188
22-23	0.03626692456479691
24-25	0.01036197844708483
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.009498480243161094
38-39	0.0025904946117712075
40-41	8.634982039237358E-4
42-43	8.634982039237358E-4
44-45	0.004317491019618679
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.006044487427466151
60-61	0.007771483835313623
62-63	0.009498480243161094
64-65	0.02504144791378834
66-67	0.006044487427466151
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.009498480243161094
80-81	0.03194943354517823
82-83	0.029358938933407016
84-85	0.012088974854932303
86-87	0.02676844432163581
88-89	0.019860458690245924
90-91	0.0
92-93	0.0017269964078474717
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.02245095330201713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	57904.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.29738878143134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59599557793882	62.28067145620337
2	7.320967940056504	10.292898590770932
3	1.3683822626212996	2.885810997513125
4	0.6289153666625722	1.768444321635811
5	0.3414813904925685	1.2002625034539927
6	0.2800638742169267	1.1812655429676706
7	0.18179584817589978	0.8945841392649904
8	0.17442574622282275	0.980933959657364
9	0.11300822994718095	0.7149765128488533
>10	0.9188060434836016	12.531085935341254
>50	0.05404741432256479	2.5386847195357833
>100	0.02211030585923105	2.7303813208068526
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	427	0.7374274661508704	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	227	0.39202818458137606	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	197	0.3402182923459519	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	152	0.2625034539928157	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	145	0.2504144791378834	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115	0.19860458690245925	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	110	0.1899696048632219	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	107	0.18478861563967947	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101	0.17442663719259463	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	88	0.1519756838905775	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	85	0.1467946946670351	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	82	0.1416137054434927	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	80	0.13815971262779772	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	80	0.13815971262779772	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	76	0.13125172699640786	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	74	0.1277977341807129	No Hit
GCACTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCT	69	0.11916275214147555	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	69	0.11916275214147555	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	67	0.11570875932578059	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	66	0.11398176291793312	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	65	0.11225476651008566	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	63	0.10880077369439071	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	61	0.10534678087869577	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	60	0.10361978447084831	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	60	0.10361978447084831	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	8.634982039237358E-4	0.0
32-33	0.0	0.0	0.0	0.00431749101961868	0.0
34-35	0.0	0.0	0.0	0.008634982039237358	0.0
36-37	0.0	0.0	0.0	0.02245095330201713	0.0
38-39	0.0	0.0	0.0	0.040584415584415584	0.0
40-41	0.0	0.0	0.0	0.05612738325504283	0.0
42-43	0.0	0.0	0.0	0.06907985631389887	0.0
44-45	0.0	0.0	0.0	0.09239430781983973	0.0
46-47	0.0	0.0	0.0	0.11829925393755181	0.0
48-49	0.0	0.0	0.0	0.14334070185134015	0.0
50-51	0.0	0.0	0.0	0.1752901353965184	0.0
52-53	0.0	0.0	0.0	0.2046490743299254	0.0
54-55	0.0	0.0	0.0	0.24264299530256977	0.0
56-57	0.0	0.0	0.0	0.2849544072948328	0.0
58-59	0.0	0.0	0.0	0.3289928156949433	0.0
60-61	0.0	0.0	0.0	0.38166620613429125	0.0
62-63	0.0	0.0	0.0	0.4369300911854103	0.0
64-65	0.0	0.0	0.0	0.48873998342083447	0.0
66-67	0.0	0.0	0.0	0.5362323846366399	0.0
68-69	0.0	0.0	0.0	0.6122202265819288	0.0
70-71	0.0	0.0	0.0	0.683027079303675	0.0
72-73	0.0	0.0	0.0	0.7780118817352859	0.0
74-75	0.0	0.0	0.0	0.8557267200884222	0.0
76-77	0.0	0.0	0.0	0.9325780602376348	0.0
78-79	0.0	0.0	0.0	1.0336073500967118	0.0
80-81	0.0	0.0	0.0	1.1579510914617297	0.0
82-83	0.0	0.0	0.0	1.2909298148659851	0.0
84-85	0.0	0.0	0.0	1.4170005526388505	0.0
86-87	0.0	0.0	0.0	1.5163028460900803	0.0
88-89	0.0	0.0	0.0	1.6371925946394033	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	120	0.0	79.14579	1
CCGTGCG	40	8.926145E-8	71.23122	9
AAACCGT	35	3.3815013E-6	67.83926	6
AACCGTG	40	7.4673644E-6	59.35935	7
TCTACAG	25	0.0050025075	56.984974	3
ACATGAG	25	0.0050025075	56.984974	3
ACCGTGC	50	4.179201E-7	56.984974	8
GTATCAA	175	0.0	54.271404	2
CATGGGA	80	4.116373E-9	47.487476	4
ATGGGAG	55	4.9125403E-5	43.170433	5
TCAACGC	310	0.0	38.296352	3
TACAAAA	75	6.8215286E-6	37.98998	2
ATCAACG	315	0.0	37.688477	2
CAAAACC	90	5.5859346E-7	36.934704	4
TATCAAC	325	0.0	36.528828	1
ACGCAGA	340	0.0	36.313953	6
CAGAGTA	340	0.0	36.313953	9
CGCAGAG	345	0.0	35.787666	7
CGTGCGC	40	1.0648726E-5	35.646393	10-11
AACGCAG	350	0.0	35.276413	5
>>END_MODULE