SRR3224551_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3224551_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57904
Sequences flagged as poor quality	0
Sequence length	101
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	347	0.5992677535230727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	0.3868471953578337	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	194	0.33503730312240954	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	139	0.24005250069079856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.23659850787510361	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	128	0.22105554020447638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114	0.1968775904946118	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	114	0.1968775904946118	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	110	0.1899696048632219	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	97	0.16751865156120477	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	94	0.16233766233766234	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	90	0.15542967670627245	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	87	0.15024868748273004	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	86	0.14852169107488256	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	85	0.1467946946670351	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	80	0.13815971262779772	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	78	0.13470571981210278	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	77	0.13297872340425532	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	74	0.1277977341807129	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	71	0.1226167449571705	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	68	0.11743575573362806	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	66	0.11398176291793312	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	62	0.10707377728654324	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	62	0.10707377728654324	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	61	0.10534678087869577	No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG	60	0.10361978447084831	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	59	0.10189278806300084	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	59	0.10189278806300084	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTA	59	0.10189278806300084	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	35	3.5335688E-8	81.420425	9
ACCGTGC	35	3.5335688E-8	81.420425	8
GGTATCA	150	0.0	73.01521	1
GGACCGG	20	0.0020649314	71.242874	6
ACTATAC	25	0.004999268	56.9943	3
TCTAAAC	25	0.004999268	56.9943	3
GTATCAA	210	0.0	52.01861	2
AACCGTG	55	8.0608334E-7	51.813	7
AAACCGT	55	8.0608334E-7	51.813	6
CGGCTTA	35	4.279582E-6	40.710213	16-17
GTGCGCG	35	4.279582E-6	40.710213	10-11
TTAATCC	50	0.0016039595	37.9962	4
ATACAAA	90	5.466245E-7	37.036697	1
ATCAACG	295	0.0	37.030197	4
TCAACGC	300	0.0	36.413025	5
GTCTAAT	120	3.5743142E-9	35.71396	1
CGTGCGC	40	1.0700034E-5	35.621437	10-11
GCGCGGC	40	1.0700034E-5	35.621437	12-13
TGCGCGG	40	1.0700034E-5	35.621437	12-13
GCGGCTT	40	1.0700034E-5	35.621437	14-15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content