##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224551_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57904
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.11379179331307	34.0	33.0	34.0	31.0	34.0
2	33.23269549599337	34.0	33.0	34.0	31.0	34.0
3	33.331945979552366	34.0	34.0	34.0	31.0	34.0
4	36.57771483835314	37.0	37.0	37.0	35.0	37.0
5	36.63757253384913	37.0	37.0	37.0	35.0	37.0
6	36.676585382702406	37.0	37.0	37.0	36.0	37.0
7	36.645689416966015	37.0	37.0	37.0	35.0	37.0
8	36.64259809339597	37.0	37.0	37.0	35.0	37.0
9	38.477911715943634	39.0	39.0	39.0	37.0	39.0
10-11	38.50110527770102	39.0	39.0	39.0	37.5	39.0
12-13	38.482367366675874	39.0	39.0	39.0	37.0	39.0
14-15	40.0346608179055	41.0	40.0	41.0	38.0	41.0
16-17	40.0359819701575	41.0	40.0	41.0	38.0	41.0
18-19	40.06612669245648	41.0	40.0	41.0	38.0	41.0
20-21	40.074675324675326	41.0	40.0	41.0	38.0	41.0
22-23	39.97101236529428	41.0	40.0	41.0	38.0	41.0
24-25	39.92524696048632	41.0	40.0	41.0	38.0	41.0
26-27	39.84726443768997	41.0	40.0	41.0	38.0	41.0
28-29	39.7844622133186	41.0	40.0	41.0	37.5	41.0
30-31	39.659220433821496	41.0	40.0	41.0	37.0	41.0
32-33	39.51279704338215	41.0	40.0	41.0	35.5	41.0
34-35	39.37912752141476	41.0	39.5	41.0	35.0	41.0
36-37	39.204320945012434	41.0	39.0	41.0	35.0	41.0
38-39	39.044375172699645	41.0	39.0	41.0	35.0	41.0
40-41	38.86883462282398	41.0	39.0	41.0	35.0	41.0
42-43	38.70620337109699	41.0	38.5	41.0	35.0	41.0
44-45	38.37127832274109	40.0	38.0	41.0	35.0	41.0
46-47	38.00698570046974	40.0	37.5	41.0	34.0	41.0
48-49	37.7952904807958	40.0	37.0	41.0	34.0	41.0
50-51	37.56517684443216	40.0	37.0	41.0	33.5	41.0
52-53	37.26253799392097	40.0	36.0	41.0	33.0	41.0
54-55	37.01441178502348	39.0	35.0	41.0	33.0	41.0
56-57	36.76661370544349	39.0	35.0	41.0	33.0	41.0
58-59	36.48084760983697	38.5	35.0	41.0	32.0	41.0
60-61	36.2222471677259	38.0	35.0	41.0	32.0	41.0
62-63	36.00704614534402	37.0	35.0	40.5	31.5	41.0
64-65	35.78166447913789	37.0	35.0	40.0	31.0	41.0
66-67	35.502115570599614	36.0	35.0	40.0	31.0	41.0
68-69	35.25383393202542	36.0	35.0	39.5	31.0	41.0
70-71	34.97811895551257	35.0	35.0	39.0	30.0	41.0
72-73	34.70098784194529	35.0	35.0	39.0	30.0	41.0
74-75	34.4223801464493	35.0	35.0	38.5	30.0	40.5
76-77	32.64347886156397	34.5	32.0	35.5	27.0	38.5
78-79	33.966833033987285	35.0	34.0	37.0	30.0	39.0
80-81	33.933510638297875	35.0	35.0	36.5	30.0	39.0
82-83	33.738714078474715	35.0	35.0	36.0	30.0	38.5
84-85	33.543753453992814	35.0	34.5	36.0	30.0	37.0
86-87	33.374559615916	35.0	34.0	35.5	30.0	37.0
88-89	33.19145482177397	35.0	34.0	35.0	29.0	36.5
90-91	33.01571566731141	35.0	34.0	35.0	29.0	36.0
92-93	32.905153357281016	35.0	34.0	35.0	29.0	36.0
94-95	32.80490985078751	35.0	34.0	35.0	29.0	36.0
96-97	32.75080305332965	35.0	34.0	35.0	29.0	36.0
98-99	32.69543554849406	35.0	34.0	35.0	29.0	35.0
100-101	31.847765266648246	34.5	32.5	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	12.0
17	43.0
18	114.0
19	195.0
20	255.0
21	373.0
22	548.0
23	479.0
24	322.0
25	267.0
26	289.0
27	319.0
28	381.0
29	459.0
30	497.0
31	568.0
32	633.0
33	832.0
34	1478.0
35	3018.0
36	8213.0
37	15955.0
38	16304.0
39	6326.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.939240418481184	19.685271613720353	11.943716709752417	22.431771258046044
2	17.059270516717326	20.23176291793313	37.64506769825919	25.063898867090355
3	18.04711246200608	27.09139264990329	31.32253384912959	23.53896103896104
4	11.28419452887538	16.957377728654325	40.60859353412545	31.149834208344846
5	11.691765681127384	40.44280187897209	35.77645758496823	12.088974854932303
6	27.464423873998346	39.84180712904117	18.68782812931749	14.005940867642996
7	23.856728378004973	33.24986184028737	23.794556507322465	19.09885327438519
8	26.317698259187623	34.33096159159989	20.494266371925946	18.857073777286544
9	25.759878419452885	12.387745233489914	21.51319425255595	40.33918209450124
10-11	25.840183752417794	24.702956617850237	28.954821773970714	20.50203785576126
12-13	25.723611494888093	23.87658883669522	29.466012710693562	20.93378695772313
14-15	23.059719535783366	26.06728378004974	26.313380768168003	24.559615915998894
16-17	22.02265819287096	27.841772589113013	28.04383116883117	22.09173804918486
18-19	21.45447637468914	26.497305885603755	29.841634429400386	22.206583310306716
20-21	23.577818458137607	24.694321635810997	29.834726443769	21.8931334622824
22-23	23.16872037096203	26.10291260459212	28.835044513716788	21.893322510729057
24-25	22.595157502072396	25.6657571152252	29.27863360044211	22.460451782260293
26-27	22.033883669521966	26.570703232937277	29.593810444874276	21.80160265266648
28-29	21.915962903476505	26.922611954475588	29.68239987565411	21.479025266393798
30-31	21.59954407294833	25.54832135949157	30.222437137330754	22.629697430229346
32-33	22.23680574744405	25.7167035092567	30.288063000828956	21.758427742470296
34-35	22.65752502007651	25.897398257445577	29.54225564948578	21.902821072992136
36-37	22.203512771358998	25.73269087957446	29.99930918950659	22.064487159559953
38-39	22.24025974025974	26.157087593257806	28.84688449848024	22.75576816800221
40-41	22.45268029842498	26.16313208068527	29.56704200055264	21.817145620337108
42-43	22.40691489361702	26.3824606244819	29.107660956065214	22.102963525835868
44-45	22.786427973090838	26.11639334352358	28.505919842482967	22.591258840902615
46-47	22.727469127800966	26.060543212437025	28.06021483075381	23.151772829008202
48-49	22.30243161094225	26.089734733351754	28.237254766510084	23.37057888919591
50-51	22.82053053329649	26.304745786128763	27.652666482453714	23.22205719812103
52-53	23.57868195634153	26.772761812655432	26.665688035368884	22.982868195634154
54-55	22.64178640508428	26.128592152528324	27.435064935064936	23.794556507322465
56-57	23.649237943093997	26.498855835240278	26.64392729156772	23.207978930098008
58-59	23.972673725666315	26.401292038761166	26.982536748829737	22.643497486742785
60-61	23.26280335089386	26.699196821832626	27.189740046636153	22.848259780637363
62-63	23.42325227963526	26.103550704614538	27.500690798563138	22.972506217187068
64-65	23.685755733628074	26.003384912959383	27.19846642718983	23.112392926222714
66-67	23.02172561481072	25.89372064106107	27.03871925946394	24.045834484664272
68-69	23.193561757391546	26.466219950262506	27.34785161646864	22.992366675877314
70-71	23.706479690522244	25.609629731970156	27.62589803813208	23.05799253937552
72-73	23.54414202818458	26.093188726167448	27.31762917933131	23.045040066316663
74-75	23.38007736943907	26.05174081237911	27.10607211936999	23.462109698811826
76-77	23.20565073224648	25.683027079303677	26.894515059408675	24.21680712904117
78-79	23.0302925632966	25.49480156125868	26.971434492763635	24.503471382681084
80-81	22.86582733191717	26.420910833635563	27.104813222112845	23.60844861233442
82-83	23.092532467532468	26.453267477203646	27.30208621166068	23.152113843603207
84-85	22.48780277190104	26.414230819049262	27.769094598678812	23.32887181037088
86-87	22.45527079303675	27.506735285990608	27.389299530256977	22.64869439071567
88-89	23.293727549046697	26.28315833103067	26.582792207792206	23.84032191213042
90-91	22.990639679469467	26.485216910748825	26.88588007736944	23.63826333241227
92-93	22.80153357281017	26.76326333241227	27.178605968499586	23.256597126277978
94-95	22.997547665100857	26.43599751312517	27.04390024868748	23.522554573086488
96-97	22.837800497374968	27.018858800773693	27.310721193699916	22.83261950815142
98-99	21.304227687206414	27.62330754352031	27.490328820116055	23.582135949157227
100-101	22.641965921266376	26.559637783845435	27.242249334669754	23.556146960218435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	1.0
4	0.5
5	1.5
6	1.5
7	10.0
8	17.0
9	17.5
10	21.5
11	21.0
12	25.5
13	29.5
14	29.5
15	34.5
16	36.5
17	45.0
18	50.5
19	55.5
20	71.0
21	94.5
22	122.5
23	163.5
24	210.0
25	303.0
26	440.5
27	561.0
28	642.0
29	692.5
30	802.5
31	934.0
32	1027.5
33	1113.0
34	1212.5
35	1273.0
36	1349.5
37	1382.0
38	1355.5
39	1390.0
40	1401.0
41	1429.0
42	1456.5
43	1502.0
44	1589.0
45	1689.0
46	1783.0
47	1862.5
48	1922.5
49	2112.5
50	2462.0
51	2379.5
52	2160.0
53	2407.0
54	2591.0
55	2452.0
56	1971.0
57	1633.0
58	1593.5
59	1526.5
60	1271.0
61	911.0
62	750.0
63	520.0
64	280.5
65	172.5
66	147.5
67	127.5
68	93.0
69	60.0
70	28.5
71	19.0
72	16.5
73	15.0
74	11.5
75	8.0
76	3.5
77	1.5
78	1.0
79	0.0
80	1.0
81	1.0
82	0.5
83	0.5
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4611080408952749
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.634982039237358E-4
24-25	0.0
26-27	0.0
28-29	0.0017269964078474717
30-31	0.0
32-33	0.0
34-35	8.634982039237358E-4
36-37	0.0017269964078474717
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009498480243161094
46-47	0.0768513401492125
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0025904946117712075
58-59	0.018996960486322188
60-61	0.015542967670627246
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0034539928156949434
80-81	0.0017269964078474717
82-83	0.0
84-85	0.0025904946117712075
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06562586349820393
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	57904.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.63083724785852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.68495218922999	60.178916827853
2	7.936587820835431	10.893893340701851
3	1.4997483643683944	3.0878695772312796
4	0.6542526421741319	1.7960762641613706
5	0.43532964267740315	1.4938518927880629
6	0.2591847005535984	1.0672837800497375
7	0.19879214896829392	0.9550290135396519
8	0.12581781580271767	0.6907985631389887
9	0.11575239053850025	0.7149765128488533
>10	0.9914443885254152	13.04054987565626
>50	0.0754906894816306	3.478170765404808
>100	0.02264720684448918	2.6025835866261398
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	347	0.5992677535230727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	0.3868471953578337	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	194	0.33503730312240954	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	139	0.24005250069079856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.23659850787510361	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	128	0.22105554020447638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114	0.1968775904946118	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	114	0.1968775904946118	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	110	0.1899696048632219	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	97	0.16751865156120477	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	94	0.16233766233766234	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	90	0.15542967670627245	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	87	0.15024868748273004	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	86	0.14852169107488256	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	85	0.1467946946670351	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	80	0.13815971262779772	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	78	0.13470571981210278	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	77	0.13297872340425532	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	74	0.1277977341807129	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	71	0.1226167449571705	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	68	0.11743575573362806	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	66	0.11398176291793312	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	62	0.10707377728654324	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	62	0.10707377728654324	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	61	0.10534678087869577	No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG	60	0.10361978447084831	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	59	0.10189278806300084	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	59	0.10189278806300084	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTA	59	0.10189278806300084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	8.634982039237358E-4	0.0
32-33	0.0	0.0	0.0	0.00431749101961868	0.0
34-35	0.0	0.0	0.0	0.008634982039237358	0.0
36-37	0.0	0.0	0.0	0.023314451505940866	0.0
38-39	0.0	0.0	0.0	0.043174910196186786	0.0
40-41	0.0	0.0	0.0	0.05958137607073777	0.0
42-43	0.0	0.0	0.0	0.07426084553744128	0.0
44-45	0.0	0.0	0.0	0.09757529704338215	0.0
46-47	0.0	0.0	0.0	0.12434374136501797	0.0
48-49	0.0	0.0	0.0	0.15024868748273001	0.0
50-51	0.0	0.0	0.0	0.18219812102790828	0.0
52-53	0.0	0.0	0.0	0.2115570599613153	0.0
54-55	0.0	0.0	0.0	0.2512779773418071	0.0
56-57	0.0	0.0	0.0	0.29531638574191765	0.0
58-59	0.0	0.0	0.0	0.3402182923459519	0.0
60-61	0.0	0.0	0.0	0.3946186791931473	0.0
62-63	0.0	0.0	0.0	0.4524730588560376	0.0
64-65	0.0	0.0	0.0	0.5060099474993092	0.0
66-67	0.0	0.0	0.0	0.5552293451229622	0.0
68-69	0.0	0.0	0.0	0.6329441834760984	0.0
70-71	0.0	0.0	0.0	0.7020240397899973	0.0
72-73	0.0	0.0	0.0	0.7952818458137607	0.0
74-75	0.0	0.0	0.0	0.8712696877590494	0.0
76-77	0.0	0.0	0.0	0.9498480243161094	0.0
78-79	0.0	0.0	0.0	1.0500138159712629	0.0
80-81	0.0	0.0	0.0	1.1743575573362808	0.0
82-83	0.0	0.0	0.0	1.310790273556231	0.0
84-85	0.0	0.0	0.0	1.4420420005526389	0.0
86-87	0.0	0.0	0.0	1.5447982868195633	0.0
88-89	0.0	0.0	0.0	1.670005526388505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	35	3.5335688E-8	81.420425	9
ACCGTGC	35	3.5335688E-8	81.420425	8
GGTATCA	150	0.0	73.01521	1
GGACCGG	20	0.0020649314	71.242874	6
ACTATAC	25	0.004999268	56.9943	3
TCTAAAC	25	0.004999268	56.9943	3
GTATCAA	210	0.0	52.01861	2
AACCGTG	55	8.0608334E-7	51.813	7
AAACCGT	55	8.0608334E-7	51.813	6
CGGCTTA	35	4.279582E-6	40.710213	16-17
GTGCGCG	35	4.279582E-6	40.710213	10-11
TTAATCC	50	0.0016039595	37.9962	4
ATACAAA	90	5.466245E-7	37.036697	1
ATCAACG	295	0.0	37.030197	4
TCAACGC	300	0.0	36.413025	5
GTCTAAT	120	3.5743142E-9	35.71396	1
CGTGCGC	40	1.0700034E-5	35.621437	10-11
GCGCGGC	40	1.0700034E-5	35.621437	12-13
TGCGCGG	40	1.0700034E-5	35.621437	12-13
GCGGCTT	40	1.0700034E-5	35.621437	14-15
>>END_MODULE