FastQCFastQC Report
Fri 10 Feb 2017
SRR3224550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270576
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC6240.23061912364733014No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA4960.1833126367453137No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG4750.17555141623795162No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT4190.15485482821831945No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA4160.15374608243155344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.1267666016202472No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA3420.12639701969132516No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA3340.12344036425994914No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC3210.1186357991839631No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA3180.1175270533971971No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA3150.11641830761043108No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG3140.11604872568150909No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.11050499674767902No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC2820.10422210395600498No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA2790.10311335816923896No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG2780.10274377624031694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3750.063.370091
AAACCGT1100.051.7907646
CCGTGCG1151.8189894E-1245.4443369
AACCGTG1151.8189894E-1245.410747
ACCGTGC1300.043.8310558
GTATCAA5950.039.0969472
CTACACC550.002582849734.5271764
TCCGCTA1850.033.3668828
CCGCTAC1900.032.5068329
GTATAAA1051.8860046E-631.6850451
GCACGTA300.0039486331.66746744-45
TGGTATC1351.1961674E-831.6499122
ATCAACG7900.031.249284
TCAACGC8150.030.2907125
ATCCGCT2051.8189894E-1230.1060127
TCAATAC856.414244E-427.9263943
CAGTCCG700.00837183327.1485619
TACTCTA700.00840209727.1284945
CTTAGAC700.00840209727.1284943
ACGCAGA9150.026.4662918
Produced by FastQC (version 0.11.5)