##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224550_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270576
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071303441546924	34.0	31.0	34.0	30.0	34.0
2	32.21673762639702	34.0	31.0	34.0	30.0	34.0
3	32.358335550824904	34.0	31.0	34.0	30.0	34.0
4	35.67960942581752	37.0	35.0	37.0	35.0	37.0
5	35.69454792738454	37.0	35.0	37.0	35.0	37.0
6	35.801804298977	37.0	37.0	37.0	35.0	37.0
7	35.75532937141506	37.0	37.0	37.0	35.0	37.0
8	35.79756149843297	37.0	37.0	37.0	35.0	37.0
9	37.52136183549169	39.0	38.0	39.0	35.0	39.0
10-11	37.583980101708946	39.0	38.5	39.0	35.0	39.0
12-13	37.57857311808881	39.0	38.0	39.0	35.0	39.0
14-15	39.0277371237656	41.0	39.0	41.0	36.0	41.0
16-17	38.84759549997043	41.0	39.0	41.0	35.5	41.0
18-19	38.9433782005795	41.0	39.0	41.0	35.5	41.0
20-21	39.01502165750104	41.0	39.5	41.0	36.0	41.0
22-23	38.96788333037668	41.0	39.0	41.0	35.5	41.0
24-25	38.89733014014547	41.0	39.0	41.0	35.0	41.0
26-27	38.69849136656614	41.0	39.0	41.0	35.0	41.0
28-29	38.60980279108273	41.0	39.0	41.0	35.0	41.0
30-31	38.50886257465555	40.0	39.0	41.0	34.0	41.0
32-33	38.27170554668559	40.0	38.0	41.0	34.0	41.0
34-35	38.248340577139146	40.0	38.0	41.0	34.0	41.0
36-37	38.21608161847318	40.0	38.0	41.0	34.0	41.0
38-39	38.07394040860978	40.0	38.0	41.0	33.5	41.0
40-41	37.94974609721483	40.0	38.0	41.0	33.0	41.0
42-43	37.77044712021761	40.0	37.5	41.0	33.0	41.0
44-45	37.534419165040504	40.0	37.0	41.0	32.0	41.0
46-47	37.30528945656673	40.0	37.0	41.0	31.0	41.0
48-49	37.03410871622021	40.0	36.0	41.0	31.0	41.0
50-51	35.7386834013364	38.5	34.5	40.0	28.5	40.5
52-53	36.08467121991603	39.0	35.0	40.0	29.5	40.5
54-55	36.51729643427355	39.0	35.0	41.0	30.0	41.0
56-57	36.342452767429485	39.0	35.0	41.0	30.0	41.0
58-59	36.189719339483176	38.5	35.0	41.0	30.0	41.0
60-61	35.93453225711076	38.0	35.0	41.0	29.5	41.0
62-63	35.64666674058305	37.0	35.0	40.5	29.0	41.0
64-65	35.3725311927148	37.0	35.0	40.0	29.0	41.0
66-67	35.12782175802732	36.0	35.0	40.0	29.0	41.0
68-69	34.82203706167583	36.0	34.5	39.0	28.0	41.0
70-71	34.52524983738395	35.0	34.0	39.0	28.0	41.0
72-73	34.20307787830406	35.0	34.0	38.5	27.0	41.0
74-75	33.94313612441606	35.0	34.0	37.5	27.0	40.0
76-77	33.60753171012951	35.0	34.0	37.0	26.0	39.0
78-79	33.3860394122169	35.0	34.0	36.5	26.0	39.0
80-81	33.113147507539466	35.0	34.0	36.0	26.0	38.5
82-83	32.86330827567855	35.0	33.0	36.0	26.0	37.0
84-85	32.65265766365088	35.0	33.0	35.0	25.0	37.0
86-87	32.438368517532965	35.0	33.0	35.0	25.0	36.5
88-89	32.29624209094672	35.0	33.0	35.0	25.0	36.0
90-91	32.11789848323576	35.0	33.0	35.0	24.5	36.0
92-93	31.978810020105257	35.0	33.0	35.0	24.0	36.0
94-95	31.83953676601029	35.0	33.0	35.0	24.0	35.0
96-97	31.701819451836084	35.0	32.5	35.0	24.0	35.0
98-99	31.661527629945006	35.0	32.0	35.0	24.0	35.0
100-101	30.451403672166045	34.0	29.5	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	24.0
8	73.0
9	89.0
10	135.0
11	295.0
12	538.0
13	639.0
14	676.0
15	690.0
16	708.0
17	831.0
18	999.0
19	1153.0
20	1520.0
21	1915.0
22	2316.0
23	2072.0
24	1864.0
25	1803.0
26	1842.0
27	1859.0
28	2124.0
29	2467.0
30	2960.0
31	3521.0
32	4750.0
33	6660.0
34	10887.0
35	19526.0
36	40851.0
37	66839.0
38	68737.0
39	19188.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49267256217865	18.529774309732776	11.658381115606572	24.319172012482003
2	17.484181893442138	20.04316716929809	37.24203181361244	25.23061912364733
3	19.985586251501434	25.32939111152176	29.374110690196805	25.310911946780006
4	13.104266453787474	16.46857075276447	37.91097510496127	32.51618768848679
5	13.374061261900538	38.783558039146115	34.44688368517533	13.395497013778016
6	30.259150848560108	37.218748152090356	18.321284962450477	14.200816036899061
7	26.532656259239552	31.987315948199395	22.20559103542073	19.27443675714032
8	26.283558039146115	33.46342617231387	20.51179705517119	19.74121873336881
9	26.974306664301338	13.388844539057418	20.08345159955059	39.55339719709065
10-11	26.115398261486604	24.12760185677961	28.34102063745491	21.415979244278873
12-13	26.56832091538052	22.82944533144107	27.805681213411386	22.796552539767013
14-15	24.043205711465262	24.822025078242415	24.985811918727713	26.148957291564606
16-17	23.562002544604553	26.956349676006745	26.270268071130577	23.21137970825813
18-19	23.073226362747473	26.117285650649418	27.48100351003011	23.328484476572996
20-21	24.445685087754974	24.563998092202805	27.581904025969706	23.408412794072518
22-23	24.323154844567394	25.79195644817403	26.492794680286476	23.392094026972103
24-25	23.543186442933422	25.57863161454993	27.021167668710184	23.857014273806463
26-27	23.382894270001774	26.35008278635208	27.22839424043522	23.03862870321093
28-29	23.043248477322454	26.426031872745547	27.473981432203892	23.056738217728107
30-31	23.229887351428065	25.399518065164685	27.708850748033825	23.661743835373425
32-33	23.40968895984862	25.707934184850096	27.631792915853588	23.250583939447697
34-35	23.921190349476674	25.52628466678493	27.15152119921944	23.401003784518952
36-37	23.22834281827541	25.424511998343906	27.7443335249421	23.60281165843858
38-39	23.20977798475054	25.874367531886744	26.99627079429494	23.91958368906777
40-41	23.78996117305066	25.49069613153484	27.37031965242396	23.349023042990545
42-43	23.60140667821035	25.767972014273756	27.31066114984671	23.319960157669186
44-45	23.65301664393511	25.695160580892924	26.849110512328632	23.80271226284333
46-47	23.750997871208092	25.55178581988055	26.603431198628115	24.093785110283246
48-49	23.422069954467506	25.816036899059785	26.954534031104018	23.807359115368694
50-51	23.331891963810538	26.1287032109278	26.441923895689197	24.09748092957247
52-53	23.960735615871325	26.28244929335935	25.747849033173676	24.00896605759565
54-55	23.739171249482585	25.722163089113593	26.25121962036544	24.287446041038375
56-57	23.78998876470936	26.342136774880252	25.984381467683754	23.883492992726627
58-59	24.09042016433981	26.193644492250602	26.35129390804215	23.364641435367435
60-61	23.99078109347912	26.339125645489798	26.112903881625392	23.55718937940569
62-63	23.738685367733638	25.92554939621407	26.61852152391022	23.71724371214208
64-65	24.346279561622765	25.611383905461967	25.981505036013786	24.060831496901482
66-67	23.55220989675936	26.061885241356876	25.63384134283063	24.752063519053127
68-69	23.53737951629117	26.201695641889895	26.770297439536396	23.490627402282538
70-71	24.019129560641	25.63143072556324	26.401824256401156	23.947615457394594
72-73	23.798858731003488	25.674302229318197	26.5940068594406	23.932832180237714
74-75	23.777977352019395	25.699803382413815	26.587723966648923	23.934495298917863
76-77	23.732518774761992	25.54975311927148	25.991773106262194	24.72595499970433
78-79	23.903906790029904	25.126326567576857	26.4178701728835	24.55189646950974
80-81	23.666596404811827	26.212664144635877	25.97229464123927	24.148444809313023
82-83	23.48962888050333	26.38659066663955	26.258835188037207	23.864945264819912
84-85	23.433846404099143	25.65236759946099	26.822072825610775	24.09171317082909
86-87	23.443009420618118	26.50531123170653	26.253108619079757	23.798570728595596
88-89	23.72368153502043	25.8722295136514	26.050243081871454	24.353845869456716
90-91	23.447201525634203	25.87794187215422	26.196336703920526	24.478519898291054
92-93	23.328645637948526	25.869425815834557	26.630421726266924	24.171506819949997
94-95	23.746008515167645	25.55714475784992	26.541526225533673	24.15532050144876
96-97	23.705539293950682	26.21370705457986	26.31404854828218	23.766705103187274
98-99	22.674758106839533	26.809301706741962	26.38520330852188	24.13073687789662
100-101	23.411068260559293	25.9803142182075	26.16093977052975	24.44767775070346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	2.5
5	2.0
6	6.0
7	12.0
8	15.0
9	19.5
10	21.5
11	27.0
12	30.0
13	27.5
14	42.5
15	50.5
16	51.0
17	62.5
18	77.0
19	87.5
20	112.5
21	150.0
22	174.5
23	206.5
24	287.5
25	413.0
26	596.5
27	899.5
28	1222.0
29	1620.5
30	2166.5
31	2815.0
32	3565.0
33	4202.5
34	4735.5
35	5184.0
36	5686.0
37	6149.5
38	6412.0
39	6639.5
40	6738.0
41	6832.5
42	6991.0
43	7273.0
44	7810.5
45	8501.0
46	9350.5
47	9746.0
48	10118.5
49	10982.5
50	12094.0
51	12246.0
52	11876.0
53	12206.5
54	12376.5
55	12013.0
56	10278.0
57	8693.0
58	8117.5
59	7474.0
60	6272.0
61	4881.5
62	4010.0
63	2939.0
64	1834.0
65	1201.5
66	919.5
67	817.5
68	667.5
69	457.0
70	279.0
71	196.5
72	173.5
73	132.0
74	94.0
75	58.5
76	35.5
77	27.5
78	15.0
79	8.5
80	9.0
81	9.5
82	5.0
83	4.5
84	5.5
85	3.5
86	1.5
87	0.5
88	3.0
89	3.0
90	1.0
91	2.0
92	2.5
93	2.0
94	2.5
95	2.0
96	2.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15781148364969547
2	0.0
3	3.6958192892200346E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03788214771450535
16-17	0.07391638578440068
18-19	0.024207616344391225
20-21	0.03954526639465437
22-23	0.06855744781503163
24-25	0.016815977765951156
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.021990124770859206
38-39	0.0033262373602980307
40-41	0.006098101827213057
42-43	0.003511028324759033
44-45	0.009978712080894092
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.014413695227958134
60-61	0.016631186801490155
62-63	0.027533853704689255
64-65	0.045828159186328425
66-67	0.015892022943646146
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017370350659334163
80-81	0.05876352669859855
82-83	0.050817515226775474
84-85	0.029751345278221278
86-87	0.05857873573413754
88-89	0.03363195553190231
90-91	0.0
92-93	0.003511028324759033
94-95	0.0
96-97	0.0
98-99	7.391638578440069E-4
100-101	0.04693690497309444
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	270576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.25183897164571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8075763803433	57.70717309278039
2	9.722881879715576	13.077633730299276
3	2.176637934456207	4.391487116028731
4	0.7255340741500786	1.951740028927326
5	0.3715042154066003	1.2492170835906133
6	0.20102485375196663	0.8111574656295535
7	0.12377898408792806	0.5827055014168665
8	0.08168002930497215	0.4394505742413832
9	0.06157321457062195	0.3726820720143051
>10	0.5743994204830809	7.980856454232958
>50	0.09858052145804619	4.618830029288676
>100	0.05427466911735127	6.584654147874729
>500	5.538231542586865E-4	0.2324127036751983
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	624	0.23061912364733014	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	496	0.1833126367453137	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	475	0.17555141623795162	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	419	0.15485482821831945	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	416	0.15374608243155344	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.1267666016202472	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	342	0.12639701969132516	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	334	0.12344036425994914	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	321	0.1186357991839631	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	318	0.1175270533971971	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	315	0.11641830761043108	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	314	0.11604872568150909	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	299	0.11050499674767902	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	282	0.10422210395600498	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	279	0.10311335816923896	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	278	0.10274377624031694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.6958192892200346E-4	0.0
22-23	0.0	0.0	0.0	9.239548223050086E-4	0.0
24-25	0.0	0.0	0.0	0.0011087457867660104	3.6958192892200346E-4
26-27	0.0	0.0	0.0	0.0014783277156880138	3.6958192892200346E-4
28-29	0.0	0.0	0.0	0.003141446395837029	3.6958192892200346E-4
30-31	0.0	0.0	0.0	0.006282892791674059	3.6958192892200346E-4
32-33	0.0	0.0	0.0	0.013489740405653126	3.6958192892200346E-4
34-35	0.0	0.0	0.0	0.02697948081130625	3.6958192892200346E-4
36-37	0.0	0.0	0.0	0.04434983147064041	3.6958192892200346E-4
38-39	0.0	0.0	0.0	0.06948140263733665	3.6958192892200346E-4
40-41	0.0	0.0	0.0	0.09368901898172786	3.6958192892200346E-4
42-43	0.0	0.0	0.0	0.1191901720773461	3.6958192892200346E-4
44-45	0.0	0.0	0.0	0.1517133818224824	3.6958192892200346E-4
46-47	0.0	0.0	0.0	0.19144343918159779	3.6958192892200346E-4
48-49	0.0	0.0	0.0	0.23098870557625215	3.6958192892200346E-4
50-51	0.0	0.0	0.0	0.26517503400153747	3.6958192892200346E-4
52-53	0.0	0.0	0.0	0.3032419726805038	3.6958192892200346E-4
54-55	0.0	0.0	0.0	0.3490701318668322	3.6958192892200346E-4
56-57	0.0	0.0	0.0	0.3980397374489977	3.6958192892200346E-4
58-59	0.0	0.0	0.0	0.45015078942700015	3.6958192892200346E-4
60-61	0.0	0.0	0.0	0.5063272426231447	3.6958192892200346E-4
62-63	0.0	0.0	0.0	0.5641668144994383	3.6958192892200346E-4
64-65	0.0	0.0	0.0	0.6275501153095617	3.6958192892200346E-4
66-67	0.0	0.0	0.0	0.6992490095204305	3.6958192892200346E-4
68-69	0.0	0.0	0.0	0.7848072260658743	3.6958192892200346E-4
70-71	0.0	0.0	0.0	0.8681479510377861	3.6958192892200346E-4
72-73	0.0	0.0	0.0	0.969413399562415	3.6958192892200346E-4
74-75	0.0	0.0	0.0	1.073450712553959	3.6958192892200346E-4
76-77	0.0	0.0	0.0	1.176748861687659	3.6958192892200346E-4
78-79	0.0	0.0	0.0	1.2983413163029982	3.6958192892200346E-4
80-81	0.0	0.0	0.0	1.4278797823901601	3.6958192892200346E-4
82-83	0.0	0.0	0.0	1.5675817515226775	3.6958192892200346E-4
84-85	0.0	0.0	0.0	1.715784105020401	3.6958192892200346E-4
86-87	0.0	0.0	0.0	1.8748891254213236	3.6958192892200346E-4
88-89	0.0	0.0	0.0	2.056169061557566	3.6958192892200346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	375	0.0	63.37009	1
AAACCGT	110	0.0	51.790764	6
CCGTGCG	115	1.8189894E-12	45.444336	9
AACCGTG	115	1.8189894E-12	45.41074	7
ACCGTGC	130	0.0	43.831055	8
GTATCAA	595	0.0	39.096947	2
CTACACC	55	0.0025828497	34.527176	4
TCCGCTA	185	0.0	33.366882	8
CCGCTAC	190	0.0	32.506832	9
GTATAAA	105	1.8860046E-6	31.685045	1
GCACGTA	30	0.00394863	31.667467	44-45
TGGTATC	135	1.1961674E-8	31.649912	2
ATCAACG	790	0.0	31.24928	4
TCAACGC	815	0.0	30.290712	5
ATCCGCT	205	1.8189894E-12	30.106012	7
TCAATAC	85	6.414244E-4	27.926394	3
CAGTCCG	70	0.008371833	27.148561	9
TACTCTA	70	0.008402097	27.128494	5
CTTAGAC	70	0.008402097	27.128494	3
ACGCAGA	915	0.0	26.466291	8
>>END_MODULE