FastQCFastQC Report
Fri 10 Feb 2017
SRR3224550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270576
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC6030.22285790313996806No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT5320.19661758618650582No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT4460.16483354029921352No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG4430.1637247945124475No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA4160.15374608243155344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.1511590089290994No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT4070.1504198450712554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.1385932233457513No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA3720.1374844775589853No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA3600.13304949441192124No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT3380.12491869197563715No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC3380.12491869197563715No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG3230.11937496304180711No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG3150.11641830761043108No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC2860.10570043167169299No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC2860.10570043167169299No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG2840.10496126781384897No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT2760.10200461238247295No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG2710.10015670273786294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4100.068.699381
AACCGTG851.8189894E-1255.863987
CCGTGCG950.054.9819229
AAACCGT903.6379788E-1252.760436
ACCGTGC1201.8189894E-1243.527358
TGGTATC1800.039.570322
GTATCAA7250.038.6424672
GAGTCGC550.002580312334.5340969
GTAGGGT550.002580312334.5340963
CGTAGTG550.002580312334.5340967
CCTATTG550.002580312334.5340963
TGCGCGG902.1100277E-1029.01823612-13
GTGCGCG902.1100277E-1029.01823610-11
GTCTAAT2650.028.8242991
ATCAACG9750.028.734144
CGTGCGC1002.3646862E-1128.49063110-11
GAATATA856.2084454E-428.0824991
TCTAATG2550.027.931992
TCAACGC10250.027.7957365
CCGTAGT700.00839388527.1339326
Produced by FastQC (version 0.11.5)