##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224550_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270576
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.11311054934658	34.0	33.0	34.0	31.0	34.0
2	33.24062740228254	34.0	33.0	34.0	31.0	34.0
3	33.35857208325942	34.0	34.0	34.0	31.0	34.0
4	36.59333791614925	37.0	37.0	37.0	35.0	37.0
5	36.6319592277216	37.0	37.0	37.0	35.0	37.0
6	36.673825468629886	37.0	37.0	37.0	35.0	37.0
7	36.64925196617586	37.0	37.0	37.0	35.0	37.0
8	36.64017133818225	37.0	37.0	37.0	35.0	37.0
9	38.484337117852284	39.0	39.0	39.0	37.0	39.0
10-11	38.49953802258885	39.0	39.0	39.0	37.0	39.0
12-13	38.48460321684111	39.0	39.0	39.0	37.0	39.0
14-15	40.06108450121223	41.0	40.0	41.0	38.0	41.0
16-17	40.058672979126015	41.0	40.0	41.0	38.0	41.0
18-19	40.059134956537164	41.0	40.0	41.0	38.0	41.0
20-21	40.06293610667613	41.0	40.0	41.0	38.0	41.0
22-23	39.98026432499557	41.0	40.0	41.0	38.0	41.0
24-25	39.940889066288214	41.0	40.0	41.0	38.0	41.0
26-27	39.85959767311218	41.0	40.0	41.0	38.0	41.0
28-29	39.8159463514872	41.0	40.0	41.0	38.0	41.0
30-31	39.71724580154929	41.0	40.0	41.0	37.0	41.0
32-33	39.633579844479925	41.0	40.0	41.0	36.5	41.0
34-35	39.530241041334044	41.0	40.0	41.0	36.0	41.0
36-37	39.392041422742594	41.0	39.5	41.0	36.0	41.0
38-39	39.25374016912069	41.0	39.0	41.0	35.0	41.0
40-41	39.08471372183786	41.0	39.0	41.0	35.0	41.0
42-43	38.958765744190174	41.0	39.0	41.0	35.0	41.0
44-45	38.665376825734725	40.5	38.0	41.0	35.0	41.0
46-47	38.38222347880078	40.0	38.0	41.0	35.0	41.0
48-49	38.222394077819175	40.0	37.5	41.0	35.0	41.0
50-51	38.040665099639284	40.0	37.0	41.0	34.5	41.0
52-53	37.77510939625096	40.0	36.5	41.0	34.0	41.0
54-55	37.56475075394714	40.0	36.0	41.0	34.0	41.0
56-57	37.333366595706934	39.0	35.0	41.0	33.5	41.0
58-59	37.047879338891846	39.0	35.0	41.0	33.0	41.0
60-61	36.793545990775236	38.5	35.0	41.0	33.0	41.0
62-63	36.56282338418781	37.5	35.0	41.0	33.0	41.0
64-65	36.28637240553486	37.0	35.0	40.0	32.5	41.0
66-67	36.00899931996925	36.5	35.0	40.0	32.0	41.0
68-69	35.74293174560937	36.0	35.0	39.5	32.0	41.0
70-71	35.45189336822187	35.5	35.0	39.0	31.5	41.0
72-73	35.15462014369345	35.0	35.0	39.0	31.0	41.0
74-75	34.844444814026375	35.0	35.0	37.5	31.0	40.0
76-77	33.03738136420082	34.5	32.0	35.5	28.0	38.5
78-79	34.351413281296196	35.0	34.5	37.0	31.0	39.0
80-81	34.30190223818816	35.0	35.0	36.0	31.0	39.0
82-83	34.10681287327775	35.0	35.0	36.0	31.0	37.0
84-85	33.91423296966472	35.0	35.0	36.0	31.0	37.0
86-87	33.731389701969135	35.0	34.5	35.0	31.0	37.0
88-89	33.582485142806455	35.0	34.0	35.0	31.0	36.0
90-91	33.450712553958965	35.0	34.0	35.0	31.0	36.0
92-93	33.34729059487907	35.0	34.0	35.0	30.5	36.0
94-95	33.239507569037904	35.0	34.0	35.0	30.0	36.0
96-97	33.18803219797765	35.0	34.0	35.0	30.0	35.0
98-99	33.16209309029625	35.0	34.0	35.0	30.0	35.0
100-101	32.30488106853527	34.5	32.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	15.0
16	49.0
17	145.0
18	288.0
19	446.0
20	688.0
21	1018.0
22	1459.0
23	1313.0
24	1017.0
25	988.0
26	1126.0
27	1367.0
28	1784.0
29	2128.0
30	2281.0
31	2466.0
32	3074.0
33	4252.0
34	6894.0
35	14320.0
36	38421.0
37	75690.0
38	83534.0
39	25782.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82839610271421	18.33381003146208	11.561105890131605	24.27668797569211
2	17.19886464431435	20.375790905327893	37.55950269055644	24.865841759801313
3	19.748240790018333	25.835994323221573	29.592425048784815	24.823339837975283
4	13.261708355508251	16.706581514990244	38.200357755307195	31.83135237419431
5	13.318623972562238	38.14639879368458	35.09697829814913	13.437998935604046
6	30.309413990893503	36.701703033528474	18.598101827213057	14.39078114836497
7	26.40773756726391	31.75780557033883	22.510496126781383	19.323960735615874
8	26.195597540062682	33.2690260777009	20.500340015374608	20.035036366861807
9	26.973567500443497	13.564765537224291	20.167346697415883	39.29432026491633
10-11	26.255839394476965	24.032249719117736	28.382968186387558	21.328942700017738
12-13	26.99260096978298	23.006844657323636	27.617379220625626	22.383175152267754
14-15	24.028553899828513	24.99094524274141	25.28734994973686	25.69315090769322
16-17	23.30639081071492	27.117334870794156	26.40736798533499	23.168906333155935
18-19	22.95029862219857	26.133138194074863	28.007472946602803	22.909090237123767
20-21	24.327216079919133	24.72932493389042	27.96615337422179	22.977305611968653
22-23	24.401872319351302	25.589165236063398	26.962913958684055	23.046048485901245
24-25	24.032102182955832	25.287583595584934	27.334347229126283	23.345966992332954
26-27	23.510280111320135	26.30122962179982	27.283596542127576	22.90489372475247
28-29	23.17068663468433	26.42793255619701	27.790631491022523	22.61074931809614
30-31	23.201643915608884	25.32195503255123	27.90923723128836	23.56716382055153
32-33	23.343392140086593	25.763788665270877	27.97481664082668	22.918002553815846
34-35	24.166886883631197	25.52884411041278	27.147669796519523	23.156599209436497
36-37	23.27121196721766	25.467480989383624	27.636585388389435	23.62472165500929
38-39	23.484529300455325	25.618864939979897	27.16223907515818	23.7343666844066
40-41	23.632546862988587	25.690933416119687	27.528125184790962	23.148394536100763
42-43	23.560293595884335	25.855766956418897	27.614237774229792	22.969701673466975
44-45	23.883711174069166	25.743622724777815	27.067035544196	23.30563055695702
46-47	23.673322939264978	25.646346447984346	26.784515427423695	23.89581518532698
48-49	23.337096944083584	25.54407582412912	27.32196612011154	23.796861111675753
50-51	23.75838950978653	25.750990479569513	26.72114304298977	23.769476967654192
52-53	24.083621607237895	26.17471616107859	26.09562562828928	23.64603660339424
54-55	23.852632901661643	25.70294482880965	26.48886080066229	23.955561468866417
56-57	24.093170410780683	26.086827962950643	26.064651571936515	23.75535005433216
58-59	24.29150321480994	26.02960804830164	26.36329016109174	23.315598575796677
60-61	24.12193873118506	26.272613508862406	26.132685635488812	23.472762124463717
62-63	23.89790668795459	25.686313642008162	26.754405416592753	23.661374253444503
64-65	23.94151735556738	25.69518360830229	26.417531488380348	23.945767547749984
66-67	23.560478386848796	25.79145970078647	26.259720004730646	24.388341907634086
68-69	23.780379634557388	26.014132812961975	26.59345248654722	23.612035065933416
70-71	24.300196617586188	25.584493820590147	26.51306841700668	23.602241144816983
72-73	23.83951274318491	25.66580184495299	26.670325527762994	23.82435988409911
74-75	23.875546981254807	25.68742238779493	26.691022115782626	23.746008515167645
76-77	23.639982186118107	25.27630920020475	26.41628676654021	24.667421847136936
78-79	24.03066966537983	25.18992979389083	26.34290666967277	24.436493871056566
80-81	23.398900075398057	26.03432090004583	26.416855161810144	24.14992386274597
82-83	23.42529797653146	26.02457728910653	26.768733253256954	23.781391481105054
84-85	23.54077118390367	25.564639225933306	26.928606539503498	23.96598305065953
86-87	23.235800662290817	26.584952102182015	26.523231920052037	23.656015315475134
88-89	23.763009283898054	25.730293891549877	25.9851206315416	24.521576193010468
90-91	23.72549671811247	25.908801963219208	26.12315948199397	24.242541836674352
92-93	23.65527615161729	26.057928271539232	26.551504937614574	23.735290639228904
94-95	23.665624445627106	25.801438412867366	26.31663562178464	24.216301519720894
96-97	23.720322571107562	26.2395777896044	26.35987670746851	23.680222931819525
98-99	22.326444326178226	26.96451274318491	26.603061616699193	24.105981313937676
100-101	23.30507142526929	26.135638666728305	26.269150755859645	24.29013915214276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	4.0
4	6.0
5	5.5
6	5.0
7	9.5
8	17.5
9	20.0
10	23.5
11	29.5
12	34.5
13	43.5
14	49.0
15	60.5
16	82.5
17	84.5
18	88.0
19	103.5
20	113.5
21	142.0
22	184.0
23	245.0
24	337.5
25	462.5
26	662.0
27	946.0
28	1230.5
29	1644.0
30	2244.0
31	2928.0
32	3564.5
33	4159.0
34	4901.5
35	5428.0
36	5751.5
37	6048.5
38	6343.0
39	6624.0
40	6720.0
41	6786.5
42	6922.5
43	7345.5
44	7918.0
45	8492.0
46	9156.5
47	9904.0
48	10371.0
49	11142.0
50	12451.0
51	12352.0
52	11336.0
53	11661.0
54	12342.0
55	11948.0
56	10272.0
57	8690.0
58	8178.5
59	7750.5
60	6408.0
61	4881.0
62	4013.5
63	2814.5
64	1637.0
65	1092.0
66	849.5
67	705.5
68	582.0
69	377.5
70	225.0
71	161.0
72	129.0
73	101.0
74	69.0
75	53.5
76	40.0
77	27.5
78	18.5
79	7.5
80	5.0
81	5.0
82	2.5
83	1.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5037401691206906
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.239548223050085E-4
22-23	9.239548223050085E-4
24-25	9.239548223050085E-4
26-27	0.001847909644610017
28-29	0.003695819289220034
30-31	9.239548223050085E-4
32-33	1.8479096446100173E-4
34-35	0.003511028324759033
36-37	0.001293536751227012
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009424339187511089
46-47	0.08481905268759979
48-49	9.239548223050085E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.006652474720596061
58-59	0.04028443025249838
60-61	0.029381763349299275
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.8479096446100173E-4
78-79	0.005728519898291053
80-81	0.0044349831470640415
82-83	3.6958192892200346E-4
84-85	0.001847909644610017
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06966619360179764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	270576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.66231106626206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.88196684713459	55.73546111032696
2	10.326084471334433	13.560691413065197
3	2.3311465191840157	4.592054036510847
4	0.8033262445489995	2.1099303102907405
5	0.3849323377700966	1.263777345106177
6	0.22095913271633721	0.8705212383210971
7	0.1253572274925292	0.5761871686213043
8	0.09612630366804853	0.5049500202481096
9	0.07904341762159088	0.46711561280484243
>10	0.5920645357884248	8.305345001111396
>50	0.1014848698346571	4.810843640854044
>100	0.05638048324147617	6.78293783668548
>500	0.0011276096648295234	0.4201852660538161
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	603	0.22285790313996806	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	532	0.19661758618650582	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	446	0.16483354029921352	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	443	0.1637247945124475	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	416	0.15374608243155344	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	409	0.1511590089290994	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	407	0.1504198450712554	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.1385932233457513	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	372	0.1374844775589853	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	360	0.13304949441192124	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	338	0.12491869197563715	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	338	0.12491869197563715	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	323	0.11937496304180711	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	315	0.11641830761043108	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC	286	0.10570043167169299	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	286	0.10570043167169299	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	284	0.10496126781384897	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	276	0.10200461238247295	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	271	0.10015670273786294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.6958192892200346E-4	0.0
22-23	0.0	0.0	0.0	9.239548223050086E-4	0.0
24-25	0.0	0.0	0.0	0.0011087457867660104	0.0
26-27	0.0	0.0	0.0	0.0014783277156880138	0.0
28-29	0.0	0.0	0.0	0.003141446395837029	0.0
30-31	0.0	0.0	0.0	0.006098101827213057	0.0
32-33	0.0	0.0	0.0	0.013120158476731121	0.0
34-35	0.0	0.0	0.0	0.02660989888238425	0.0
36-37	0.0	0.0	0.0	0.0434258766483354	0.0
38-39	0.0	0.0	0.0	0.06892702974395364	0.0
40-41	0.0	0.0	0.0	0.09313464608834486	0.0
42-43	0.0	0.0	0.0	0.1188205901484241	0.0
44-45	0.0	0.0	0.0	0.1518981727869434	0.0
46-47	0.0	0.0	0.0	0.1919978120749808	0.0
48-49	0.0	0.0	0.0	0.23135828750517415	0.0
50-51	0.0	0.0	0.0	0.2662837797883035	0.0
52-53	0.0	0.0	0.0	0.3065682100408018	0.0
54-55	0.0	0.0	0.0	0.35184199633374724	0.0
56-57	0.0	0.0	0.0	0.4006268109514517	0.0
58-59	0.0	0.0	0.0	0.4536618177517592	0.0
60-61	0.0	0.0	0.0	0.5109470167346697	0.0
62-63	0.0	0.0	0.0	0.5693409615043463	0.0
64-65	0.0	0.0	0.0	0.6325394713500089	0.0
66-67	0.0	0.0	0.0	0.7055319023121045	0.0
68-69	0.0	0.0	0.0	0.7912749098220093	0.0
70-71	0.0	0.0	0.0	0.8772027082963751	0.0
72-73	0.0	0.0	0.0	0.9810552303234581	0.0
74-75	0.0	0.0	0.0	1.089527526462066	0.0
76-77	0.0	0.0	0.0	1.19541274909822	0.0
78-79	0.0	0.0	0.0	1.3197770681804744	0.0
80-81	0.0	0.0	0.0	1.4520873987345515	0.0
82-83	0.0	0.0	0.0	1.5936372775116787	0.0
84-85	0.0	0.0	0.0	1.7446114954763172	0.0
86-87	0.0	0.0	0.0	1.9087058719176868	0.0
88-89	0.0	0.0	0.0	2.093496836378688	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	410	0.0	68.69938	1
AACCGTG	85	1.8189894E-12	55.86398	7
CCGTGCG	95	0.0	54.981922	9
AAACCGT	90	3.6379788E-12	52.76043	6
ACCGTGC	120	1.8189894E-12	43.52735	8
TGGTATC	180	0.0	39.57032	2
GTATCAA	725	0.0	38.642467	2
GAGTCGC	55	0.0025803123	34.534096	9
GTAGGGT	55	0.0025803123	34.534096	3
CGTAGTG	55	0.0025803123	34.534096	7
CCTATTG	55	0.0025803123	34.534096	3
TGCGCGG	90	2.1100277E-10	29.018236	12-13
GTGCGCG	90	2.1100277E-10	29.018236	10-11
GTCTAAT	265	0.0	28.824299	1
ATCAACG	975	0.0	28.73414	4
CGTGCGC	100	2.3646862E-11	28.490631	10-11
GAATATA	85	6.2084454E-4	28.082499	1
TCTAATG	255	0.0	27.93199	2
TCAACGC	1025	0.0	27.795736	5
CCGTAGT	70	0.008393885	27.133932	6
>>END_MODULE