FastQCFastQC Report
Fri 10 Feb 2017
SRR3224549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794438
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG20520.2582958015603483No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA16490.20756811733577699No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA15480.19485472749289434No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA11600.14601517047271154No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10670.13430878180550276No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC10610.13355353092374736No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG10530.1325465297480735No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA9920.1248681457835602No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT9870.12423877004876403No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC9530.11995901505215008No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA9450.11895201387647619No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA8950.1126582565285145No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA8060.10145536844914267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.049.1059151
GGTATCA7500.048.1759221
CCGTGCG953.8380676E-1045.0114569
AAACCGT1208.731149E-1139.5709846
ATCAACG19150.036.9466933
TCAACGC19500.036.2835434
ACCGTGC1553.6379788E-1133.6991588
CCGTTTA600.00394530331.674739
TCGCACC7550.030.8550931
GTACATG47750.030.7654191
CGCACCA7800.030.4392172
TATCAAC23600.030.3824632
AACCGTG1751.5279511E-1029.8478267
CGCAGAG24750.028.5870388
ACGCAGA25200.028.2649887
ACATGGG49450.027.8477273
CTGTGCG9150.027.5206649
CGGTCTA953.9835868E-1027.49141928-29
GCCCATA8350.026.7601851
TACATGG54000.026.4685882
Produced by FastQC (version 0.11.5)