##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3224549_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 794438 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25757453696827 34.0 31.0 34.0 31.0 34.0 2 32.420509844695246 34.0 31.0 34.0 31.0 34.0 3 32.56144972924256 34.0 31.0 34.0 31.0 34.0 4 35.844817342574245 37.0 37.0 37.0 35.0 37.0 5 35.860394392010456 37.0 37.0 37.0 35.0 37.0 6 35.96469579753234 37.0 37.0 37.0 35.0 37.0 7 35.92203419272492 37.0 37.0 37.0 35.0 37.0 8 35.962122909528496 37.0 37.0 37.0 35.0 37.0 9 37.69638411052845 39.0 39.0 39.0 35.0 39.0 10-11 37.77467593443416 39.0 39.0 39.0 36.0 39.0 12-13 37.7856793104056 39.0 39.0 39.0 37.0 39.0 14-15 39.28833779350937 41.0 40.0 41.0 37.0 41.0 16-17 39.13223498875935 41.0 40.0 41.0 36.5 41.0 18-19 39.22309796359187 41.0 40.0 41.0 36.5 41.0 20-21 39.29352825519423 41.0 40.0 41.0 37.0 41.0 22-23 39.25075336275455 41.0 40.0 41.0 37.0 41.0 24-25 39.19991050277051 41.0 40.0 41.0 36.0 41.0 26-27 39.05040481447262 41.0 39.5 41.0 36.0 41.0 28-29 38.9816990123836 41.0 39.0 41.0 36.0 41.0 30-31 38.92737759271334 41.0 39.0 41.0 35.0 41.0 32-33 38.746155773011864 41.0 39.0 41.0 35.0 41.0 34-35 38.74963118581941 41.0 39.0 41.0 35.0 41.0 36-37 38.74679521875842 41.0 39.0 41.0 35.0 41.0 38-39 38.64276570355396 41.0 39.0 41.0 35.0 41.0 40-41 38.56235162467052 40.5 39.0 41.0 35.0 41.0 42-43 38.433022463678725 40.0 38.5 41.0 34.5 41.0 44-45 38.27903624952482 40.0 38.0 41.0 34.0 41.0 46-47 38.125208008680346 40.0 38.0 41.0 34.0 41.0 48-49 37.94020112330981 40.0 37.5 41.0 33.0 41.0 50-51 36.67940544133086 39.0 35.5 40.0 31.5 40.5 52-53 36.991539301997136 39.0 36.0 40.0 32.5 40.5 54-55 37.459492748332785 40.0 36.0 41.0 33.0 41.0 56-57 37.332137309645304 40.0 35.5 41.0 33.0 41.0 58-59 37.20554845060282 40.0 35.0 41.0 33.0 41.0 60-61 36.982955875725985 39.0 35.0 41.0 32.5 41.0 62-63 36.72371726931492 39.0 35.0 41.0 32.0 41.0 64-65 36.49758130905118 38.5 35.0 41.0 32.0 41.0 66-67 36.28665421341879 37.5 35.0 41.0 31.0 41.0 68-69 36.011409323320386 37.0 35.0 40.0 31.0 41.0 70-71 35.731909853254756 37.0 35.0 40.0 31.0 41.0 72-73 35.416276034127264 36.0 35.0 39.0 30.5 41.0 74-75 35.12788789055911 36.0 35.0 39.0 30.0 41.0 76-77 34.78051024749571 35.0 35.0 38.5 30.0 40.0 78-79 34.504408147646515 35.0 34.5 37.0 30.0 39.0 80-81 34.164343221245716 35.0 34.0 37.0 29.5 39.0 82-83 33.86640430090202 35.0 34.0 36.5 29.0 39.0 84-85 33.59331816957396 35.0 34.0 36.0 29.0 37.0 86-87 33.33428788653111 35.0 34.0 36.0 29.0 37.0 88-89 33.145727545761915 35.0 34.0 35.0 29.0 36.5 90-91 32.93669424171553 35.0 34.0 35.0 28.5 36.0 92-93 32.776419934595275 35.0 34.0 35.0 27.0 36.0 94-95 32.610712226756526 35.0 33.5 35.0 27.0 36.0 96-97 32.467284419929555 35.0 33.0 35.0 27.0 35.5 98-99 32.39866358356473 35.0 33.0 35.0 26.0 35.0 100-101 31.183541698659937 34.5 31.0 35.0 22.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 343.0 8 1348.0 9 963.0 10 700.0 11 880.0 12 1182.0 13 1344.0 14 1273.0 15 1278.0 16 1455.0 17 1565.0 18 1760.0 19 1941.0 20 2380.0 21 3089.0 22 3500.0 23 3549.0 24 3527.0 25 3629.0 26 3786.0 27 4051.0 28 4662.0 29 5277.0 30 6485.0 31 8295.0 32 11253.0 33 16012.0 34 26508.0 35 47395.0 36 103834.0 37 179294.0 38 250727.0 39 91147.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.159763500097704 20.717946131978543 13.418848134537683 23.70344223338607 2 15.219564018840991 20.854742597911983 39.54380329239034 24.381890090856682 3 19.223299015529236 28.45511978923439 28.959753888602872 23.361827306633504 4 12.45534579161621 17.066152424732955 39.96133115485412 30.51717062879671 5 12.746243256239001 39.99682794629663 34.45366409965283 12.803264697811533 6 28.031765852086632 38.75481787124986 19.6520055687165 13.561410707947003 7 26.138226016378873 32.036106027153785 23.694234163018386 18.131433793448952 8 25.612067902089276 35.448455386071664 21.120968533730764 17.818508178108296 9 27.14333906484836 15.833582985708135 20.786140642819202 36.236937306624306 10-11 25.111777130499803 26.26070253437021 28.34613903161732 20.28138130351267 12-13 25.651466823087514 23.827158318207335 28.58309899576808 21.938275862937072 14-15 22.778482941337945 25.53151703347764 25.718700182083303 25.971299843101107 16-17 22.277333736434695 28.88007104661489 26.82060099893556 22.02199421801485 18-19 22.48717018951355 27.868199193195036 27.476568913331416 22.168061703959992 20-21 23.63843681843138 26.417284958016634 27.106975736786698 22.83730248676529 22-23 23.662242375576653 27.296270054949378 26.343646182671264 22.697841386802704 24-25 22.79765479333401 27.271903220567516 26.867522513505588 23.06291947259288 26-27 22.535868123126036 28.001933442257293 27.279473036284767 22.182725398331904 28-29 22.478406118539144 27.70700797293181 27.545384284236153 22.269201624292897 30-31 22.718450023790403 27.277270221212984 27.24284336851963 22.761436386476984 32-33 22.65985514288088 27.21087108119199 27.569237624584925 22.560036151342207 34-35 22.99323546960241 26.977372683582608 27.108975149728487 22.920416697086495 36-37 22.3466127808781 27.051861923961862 27.687726819196186 22.913798475963855 38-39 22.580371068303606 27.31049119534144 27.09517389656965 23.0139638397853 40-41 22.91946361527834 26.78751372904452 27.781795522993985 22.51122713268315 42-43 22.72900643244672 27.085195302173943 27.575370400674714 22.610427864704626 44-45 22.707728408878474 26.78931224770992 27.223063932266506 23.27989541114511 46-47 22.86515750757139 26.590873044844283 27.274186280112485 23.269783167471846 48-49 22.144459353656295 27.171157472326353 27.31201176177373 23.372371412243623 50-51 22.251893791586003 27.419572074850397 27.16417140167011 23.16436273189349 52-53 22.46934310795808 27.02998849501157 27.057492214622158 23.443176182408195 54-55 22.606106455129286 26.621083080114495 27.33076715867066 23.442043306085562 56-57 22.62007859644176 26.968309673001546 27.113758405312936 23.297853325243757 58-59 22.735819304919172 26.929078741877387 27.401941684013998 22.933160269189443 60-61 22.724998032890078 27.142717759068375 27.11709811944291 23.01518608859863 62-63 22.277028571140775 26.81732361886427 27.793324313894114 23.112323496100846 64-65 23.10043390408522 26.33163721815282 27.038754164465452 23.529174713296506 66-67 22.44706418518691 26.81739268372212 26.692441625509407 24.043101505581564 68-69 22.479538994861777 26.810399301141185 27.846918198776997 22.863143505220044 70-71 22.84180766781045 26.494830307714384 27.419257386983 23.244104637492164 72-73 22.659225767146083 26.897630777983935 27.45714580621773 22.985997648652255 74-75 22.704855507918804 26.359388649586247 27.588433584496208 23.347322257998737 76-77 22.378209501559592 26.21601685719968 27.29086473708458 24.114908904156145 78-79 22.76515907372704 25.554653994736253 27.504606248383013 24.17558068315369 80-81 22.24860842683433 26.797174472008265 27.030808885160813 23.923408215996588 82-83 22.24757066359043 26.956440701019087 27.42115046521351 23.374838170176968 84-85 22.547735172333905 25.973448736746874 27.700030030237993 23.778786060681227 86-87 22.23918334703028 26.921335436680742 27.483382610858687 23.35609860543029 88-89 22.541187966213528 26.171231634775687 27.27125631481659 24.016324084194196 90-91 22.356558976282606 26.04979872564001 27.35845969100169 24.235182607075696 92-93 22.260748174125833 26.115098676508726 27.829192335482507 23.794960813882938 94-95 22.70384850674313 25.70848826466005 27.744204078858264 23.843459149738557 96-97 22.98989977820799 26.000078042591113 27.59208396375803 23.417938215442867 98-99 22.027583177194817 26.169843039096413 27.829516252776347 23.97305753093242 100-101 22.70925265469786 25.806547079328617 27.27884654133672 24.205353724636804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 1.5 3 2.0 4 4.0 5 3.5 6 3.5 7 6.0 8 16.5 9 27.0 10 23.0 11 19.5 12 21.0 13 26.5 14 30.0 15 27.5 16 39.5 17 46.0 18 46.5 19 56.0 20 60.0 21 71.5 22 96.0 23 96.5 24 108.5 25 164.0 26 233.5 27 324.0 28 458.0 29 743.5 30 1414.0 31 2938.0 32 5568.5 33 9003.5 34 13582.0 35 20302.5 36 30779.0 37 39997.5 38 42876.0 39 42584.5 40 39413.5 41 35718.0 42 33011.5 43 30261.0 44 28421.0 45 27199.5 46 26116.0 47 23555.5 48 22336.0 49 23785.0 50 25273.5 51 24958.0 52 24267.0 53 25160.5 54 26880.5 55 27514.0 56 23360.0 57 19600.0 58 18771.5 59 17995.0 60 15664.5 61 11938.0 62 9423.0 63 6682.5 64 4058.5 65 2652.0 66 2164.5 67 1960.5 68 1445.5 69 911.0 70 598.0 71 400.5 72 295.0 73 236.0 74 175.5 75 125.5 76 88.5 77 52.0 78 34.5 79 22.0 80 13.5 81 10.0 82 7.0 83 10.0 84 10.5 85 9.0 86 8.5 87 5.0 88 5.5 89 3.5 90 4.0 91 6.5 92 4.5 93 3.0 94 2.0 95 1.0 96 2.5 97 3.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15142780179195858 2 0.0 3 1.2587514695923407E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.03725904349993328 16-17 0.07464396214682581 18-19 0.02441977851009141 20-21 0.0395247961451995 22-23 0.0661473897270775 24-25 0.017244895133415067 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.02083233682175324 38-39 0.0036503792618177884 40-41 0.0057273191866451505 42-43 0.003524504114858554 44-45 0.01126582565285145 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.016049081237302344 60-61 0.015797330943383876 62-63 0.028636595933225754 64-65 0.04638499165447776 66-67 0.015986143663822727 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.01718195755993545 80-81 0.058217255468645764 82-83 0.04959480790193822 84-85 0.030021222549777328 86-87 0.05859488090952346 88-89 0.034489790266830134 90-91 0.0 92-93 0.0030210035270216177 94-95 0.0 96-97 0.0 98-99 5.664381613165534E-4 100-101 0.047077304962753544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 794438.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.90377350415431 #Duplication Level Percentage of deduplicated Percentage of total 1 67.64384099251134 26.316006689236897 2 15.184132038546473 11.814400673695696 3 6.211971170352799 7.250073582772249 4 3.240073490151495 5.042043407906741 5 1.9206252250036497 3.73597843699537 6 1.2656529114686275 2.9543204521589366 7 0.8025086208160013 2.185442953354988 8 0.6017874524155278 1.8729442197132584 9 0.4415598978670198 1.5460511629622442 >10 2.3387394036199662 16.2439495162743 >50 0.1778355366851708 4.824549718587709 >100 0.15749265339030727 12.005332926964304 >500 0.01148383930970464 2.9847710266891125 >1k 0.002296767861940928 1.2241352326882176 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG 2052 0.2582958015603483 No Hit ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA 1649 0.20756811733577699 No Hit GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA 1548 0.19485472749289434 No Hit TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA 1160 0.14601517047271154 No Hit GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC 1067 0.13430878180550276 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1061 0.13355353092374736 No Hit GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG 1053 0.1325465297480735 No Hit CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA 992 0.1248681457835602 No Hit GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT 987 0.12423877004876403 No Hit GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC 953 0.11995901505215008 No Hit GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA 945 0.11895201387647619 No Hit CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA 895 0.1126582565285145 No Hit ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA 806 0.10145536844914267 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2587514695923407E-4 0.0 10-11 0.0 0.0 0.0 1.2587514695923407E-4 0.0 12-13 0.0 0.0 0.0 1.2587514695923407E-4 0.0 14-15 0.0 0.0 0.0 2.5175029391846814E-4 0.0 16-17 0.0 0.0 0.0 3.776254408777022E-4 0.0 18-19 0.0 0.0 0.0 0.0010070011756738726 0.0 20-21 0.0 0.0 0.0 0.001258751469592341 0.0 22-23 0.0 0.0 0.0 0.002580440512664299 0.0 24-25 0.0 0.0 0.0 0.004531505290532426 0.0 26-27 0.0 0.0 0.0 0.006545507641880171 0.0 28-29 0.0 0.0 0.0 0.01315395285723996 0.0 30-31 0.0 0.0 0.0 0.032349912768523156 0.0 32-33 0.0 0.0 0.0 0.07086770773804879 0.0 34-35 0.0 0.0 0.0 0.1197072647582316 0.0 36-37 0.0 0.0 0.0 0.1928407251415466 0.0 38-39 0.0 0.0 0.0 0.311918614164982 0.0 40-41 0.0 0.0 0.0 0.45623447015374385 0.0 42-43 0.0 0.0 0.0 0.5967740717337288 0.0 44-45 0.0 0.0 0.0 0.7471948723500135 0.0 46-47 0.0 0.0 0.0 0.9208396375802769 0.0 48-49 0.0 0.0 0.0 1.106694292065586 0.0 50-51 0.0 0.0 0.0 1.3012972692645617 0.0 52-53 0.0 0.0 0.0 1.4921239920547609 0.0 54-55 0.0 0.0 0.0 1.6719366394860267 0.0 56-57 0.0 0.0 0.0 1.8786865683665686 0.0 58-59 0.0 0.0 0.0 2.1164017833990822 0.0 60-61 0.0 0.0 0.0 2.380991342307392 0.0 62-63 0.0 0.0 0.0 2.659427167381218 0.0 64-65 0.0 0.0 0.0 2.907778832331787 0.0 66-67 0.0 0.0 0.0 3.1861517198321327 0.0 68-69 0.0 0.0 0.0 3.511727787442192 0.0 70-71 0.0 0.0 0.0 3.8693390799533756 0.0 72-73 0.0 0.0 0.0 4.2287126245219895 0.0 74-75 0.0 0.0 0.0 4.582044162036559 0.0 76-77 0.0 0.0 0.0 4.948718465128808 0.0 78-79 0.0 0.0 0.0 5.360330195685504 0.0 80-81 0.0 0.0 0.0 5.836893502073163 0.0 82-83 0.0 0.0 0.0 6.33384858226822 0.0 84-85 0.0 0.0 0.0 6.820859525853496 0.0 86-87 0.0 0.0 0.0 7.316996417593318 0.0 88-89 0.0 0.0 0.0 7.877518447002787 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1520 0.0 49.105915 1 GGTATCA 750 0.0 48.175922 1 CCGTGCG 95 3.8380676E-10 45.011456 9 AAACCGT 120 8.731149E-11 39.570984 6 ATCAACG 1915 0.0 36.946693 3 TCAACGC 1950 0.0 36.283543 4 ACCGTGC 155 3.6379788E-11 33.699158 8 CCGTTTA 60 0.003945303 31.67473 9 TCGCACC 755 0.0 30.855093 1 GTACATG 4775 0.0 30.765419 1 CGCACCA 780 0.0 30.439217 2 TATCAAC 2360 0.0 30.382463 2 AACCGTG 175 1.5279511E-10 29.847826 7 CGCAGAG 2475 0.0 28.587038 8 ACGCAGA 2520 0.0 28.264988 7 ACATGGG 4945 0.0 27.847727 3 CTGTGCG 915 0.0 27.520664 9 CGGTCTA 95 3.9835868E-10 27.491419 28-29 GCCCATA 835 0.0 26.760185 1 TACATGG 5400 0.0 26.468588 2 >>END_MODULE