FastQCFastQC Report
Fri 10 Feb 2017
SRR3224549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794438
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT19990.25162441877150893No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA13090.1647705673696374No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT11950.15042080061628474No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC11400.14349766753352686No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG11240.1414836651821791No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA10810.13607103386293204No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA10590.1333017806298289No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG10590.1333017806298289No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG10170.12801502445754107No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG9720.12235064284437551No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT9480.11932963931735391No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA8650.10888200211973748No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT8590.10812675123798207No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA8490.10686799976838972No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT8350.10510574771096044RNA PCR Primer, Index 46 (95% over 21bp)
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG8330.10485399741704199No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA8220.1034693708004904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.043.9352151
CGTTATA459.3723426E-442.449481
GTATCAA17200.041.369731
CCGTGCG704.314028E-640.7067079
ATCAACG21100.033.761493
TCAACGC21750.032.7525254
AATGCCG953.493938E-529.9944159
TCTATAC3100.029.1074833
GTACATG49900.028.0409011
TATCAAC25950.027.6345482
CAACGCA26400.027.1635025
CGCAGAG26100.027.111818
AACGCAG26750.026.6305566
CGATTAT2007.185008E-1026.1201362
ACATGGG49150.026.0887323
ACGCAGA27600.025.6383427
ACCGTGC1301.0030466E-525.5721638
TATAATA1551.4120069E-624.5115642
TACATGG55750.024.277992
CATGGGA26450.024.2393424
Produced by FastQC (version 0.11.5)