##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224549_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794438
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16583672986438	34.0	33.0	34.0	31.0	34.0
2	33.28957199932531	34.0	33.0	34.0	31.0	34.0
3	33.408715343425165	34.0	34.0	34.0	31.0	34.0
4	36.62537919888022	37.0	37.0	37.0	35.0	37.0
5	36.66335573071782	37.0	37.0	37.0	35.0	37.0
6	36.71197248872788	37.0	37.0	37.0	36.0	37.0
7	36.69196715162165	37.0	37.0	37.0	36.0	37.0
8	36.68520387997553	37.0	37.0	37.0	36.0	37.0
9	38.53822450587711	39.0	39.0	39.0	38.0	39.0
10-11	38.562659389404836	39.0	39.0	39.0	37.5	39.0
12-13	38.564653881108406	39.0	39.0	39.0	38.0	39.0
14-15	40.17941110571246	41.0	40.0	41.0	38.0	41.0
16-17	40.188247541029	41.0	40.0	41.0	38.0	41.0
18-19	40.19457402591517	41.0	40.0	41.0	38.5	41.0
20-21	40.20482152162913	41.0	40.0	41.0	39.0	41.0
22-23	40.13477766672793	41.0	40.0	41.0	38.5	41.0
24-25	40.11083936065495	41.0	40.0	41.0	38.0	41.0
26-27	40.06282113896868	41.0	40.0	41.0	38.0	41.0
28-29	40.03347775408528	41.0	40.0	41.0	38.0	41.0
30-31	39.98435938361458	41.0	40.0	41.0	38.0	41.0
32-33	39.95217247916137	41.0	40.0	41.0	38.0	41.0
34-35	39.88346667707235	41.0	40.0	41.0	38.0	41.0
36-37	39.78264571936388	41.0	40.0	41.0	37.5	41.0
38-39	39.690479936760326	41.0	40.0	41.0	37.0	41.0
40-41	39.58126247737394	41.0	40.0	41.0	37.0	41.0
42-43	39.5028252676735	41.0	39.5	41.0	36.0	41.0
44-45	39.292958040778515	41.0	39.0	41.0	35.5	41.0
46-47	39.10200292533842	41.0	39.0	41.0	35.0	41.0
48-49	39.01502760441973	41.0	39.0	41.0	35.0	41.0
50-51	38.87153245438914	40.5	38.5	41.0	35.0	41.0
52-53	38.653284460209605	40.0	37.5	41.0	35.0	41.0
54-55	38.477814505348434	40.0	37.0	41.0	35.0	41.0
56-57	38.26376067106558	40.0	36.5	41.0	35.0	41.0
58-59	38.01760300992652	40.0	36.0	41.0	35.0	41.0
60-61	37.78573280734305	39.5	35.0	41.0	34.0	41.0
62-63	37.58279500728817	39.0	35.0	41.0	34.0	41.0
64-65	37.34787107363948	39.0	35.0	41.0	34.0	41.0
66-67	37.078107416815406	38.5	35.0	41.0	34.0	41.0
68-69	36.818875104161684	37.5	35.0	40.5	33.0	41.0
70-71	36.52171346285047	37.0	35.0	40.0	33.0	41.0
72-73	36.220276472172785	36.5	35.0	39.5	33.0	41.0
74-75	35.89692084215508	36.0	35.0	39.0	33.0	41.0
76-77	34.00597025822027	34.5	33.0	36.5	29.5	39.0
78-79	35.26430445170045	35.0	35.0	37.0	33.0	39.0
80-81	35.137123350091514	35.0	35.0	37.0	33.0	39.0
82-83	34.88125882699468	35.0	35.0	37.0	33.0	39.0
84-85	34.63175981007958	35.0	35.0	36.0	33.0	37.0
86-87	34.41643967181832	35.0	35.0	36.0	33.0	37.0
88-89	34.23044026091401	35.0	35.0	36.0	33.0	37.0
90-91	34.06654012018559	35.0	35.0	35.0	32.5	36.0
92-93	33.961180734053514	35.0	35.0	35.0	32.0	36.0
94-95	33.84799065502909	35.0	35.0	35.0	32.0	36.0
96-97	33.79243251203996	35.0	35.0	35.0	32.0	36.0
98-99	33.75076595026925	35.0	34.0	35.0	32.0	35.5
100-101	32.94880594835595	34.5	33.0	35.0	29.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	8.0
15	24.0
16	63.0
17	157.0
18	265.0
19	401.0
20	634.0
21	898.0
22	1266.0
23	1368.0
24	1396.0
25	2121.0
26	2679.0
27	3914.0
28	5153.0
29	5039.0
30	5063.0
31	5617.0
32	6690.0
33	9053.0
34	14809.0
35	31046.0
36	92368.0
37	198714.0
38	285573.0
39	120102.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4780316789008	21.023695939212125	13.437226502623044	23.06104587926403
2	15.013884028709604	20.968030230175295	40.67856774222784	23.339517998887263
3	18.687046692127012	29.0952849687452	29.430112859656766	22.787555479471024
4	12.280127587048959	17.681052517628814	40.388929029074646	29.649890866247585
5	12.442632401773329	39.52467027005254	35.4918823117726	12.540815016401533
6	27.54009752806386	38.83852484397776	20.10087634277313	13.520501285185249
7	25.93380477771708	31.959448062655614	24.136307679139215	17.970439480488093
8	25.428038437234875	35.565896898184626	21.219151148358968	17.78691351622153
9	26.8508052233151	15.993192672052444	21.10687051727133	36.049131587361124
10-11	24.964566146130977	26.359388649586247	28.761967579597147	19.91407762468563
12-13	25.520871358117308	24.111006774600412	28.747429000123358	21.620692867158922
14-15	22.54505700885406	26.04898053718478	25.944881790649493	25.461080663311677
16-17	22.00391975207631	29.364280157797083	26.951442403309006	21.6803576868176
18-19	22.136718032118303	27.962408646112095	28.022262278491212	21.878611043278394
20-21	23.30877702007408	26.899327507371613	27.46671366038021	22.3251818121741
22-23	23.476913994472778	27.545546721145875	26.70733725941129	22.270202024970057
24-25	22.9852517919957	27.304985741447858	27.01868181872537	22.69108064783107
26-27	22.533102391780762	28.296109532758752	27.359210200030464	21.81157787543003
28-29	22.414397539820257	27.72875838590356	27.926199642881194	21.930644431394985
30-31	22.73609546407195	27.30437354849673	27.565124900086225	22.394406087345097
32-33	22.8058990203125	27.300843238670907	27.87747801273103	22.015779728285565
34-35	23.132160215103845	27.220618650137773	27.123565124677906	22.523656010080476
36-37	22.196879151947023	27.21153428583836	27.723661511407844	22.86792505080677
38-39	22.463489913624475	27.343543486087018	27.25278750512941	22.940179095159095
40-41	22.3837480080258	27.210115830310233	27.988653614253096	22.417482547410874
42-43	22.652113821342887	27.186828928122775	27.888708747567463	22.272348502966878
44-45	22.549626815411784	26.904097414458338	27.52288265424942	23.023393115880456
46-47	22.815559051864767	26.47883172325355	27.541559961353045	23.16404926352864
48-49	22.089276251112427	26.800057399428272	27.76030200155331	23.35036434790599
50-51	22.37506262288561	27.020296108695707	27.522726757783488	23.08191451063519
52-53	22.41283927307057	27.093761309102355	27.60462591456219	22.88877350326489
54-55	22.57300129147901	26.693713039909973	27.578804135753828	23.15448153285719
56-57	23.004903164082908	26.843344222259987	27.03386896781787	23.117883645839235
58-59	22.699772390279836	26.88099128283105	27.504761544975743	22.91447478191337
60-61	22.652921178544446	27.532674389322587	27.01580206497104	22.798602367161923
62-63	22.201795483096227	27.158255269763025	27.6656579871557	22.974291259985048
64-65	22.563623593030545	26.978253809611324	27.11640178339908	23.34172081395905
66-67	22.439007197540903	27.14591950536102	26.84904297125767	23.566030325840405
68-69	22.584896492866655	27.005631654074957	27.51410431021678	22.89536754284161
70-71	22.895052854974207	27.023883550384042	27.13326905309162	22.947794541550127
72-73	22.39803483720567	27.24240280550528	27.24340980668095	23.116152550608103
74-75	22.61032327255242	26.62863558893205	27.259899450932608	23.501141687582923
76-77	22.43369986147414	26.486131994187073	27.244782937339863	23.835385206998925
78-79	22.7721095928348	25.973099025044217	27.006841684552395	24.24794969756859
80-81	22.020280727769844	27.16586553742796	27.00221991579803	23.811633819004165
82-83	22.16662701574582	27.156789811097976	27.31665225387163	23.359930919284572
84-85	22.450974345160247	26.35817333412216	27.392057963498402	23.79879435721919
86-87	21.95615013380528	27.11810109788303	27.351662433065894	23.5740863352458
88-89	22.51018959314635	26.314325346974844	26.953393468086873	24.222091591791933
90-91	22.44624501849106	26.470347591630812	26.903924535331896	24.179482854546233
92-93	22.458909605853197	26.40937770435056	27.37968688537487	23.75202580442137
94-95	22.467895543768044	26.029973389993934	27.17745122967431	24.32467983656371
96-97	22.723547967242254	26.3774517331749	27.19526256296904	23.70373773661381
98-99	21.48153789219549	26.602705308658447	27.62311218748348	24.292644611662585
100-101	22.03459427459402	26.44651364908822	27.209943098621707	24.308948977696048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	2.0
3	3.5
4	2.5
5	3.5
6	7.0
7	9.5
8	18.0
9	24.5
10	21.0
11	15.5
12	18.5
13	27.0
14	27.0
15	26.0
16	32.0
17	38.5
18	46.5
19	52.5
20	51.5
21	59.0
22	71.0
23	89.5
24	118.0
25	160.0
26	211.0
27	294.5
28	466.0
29	800.5
30	1566.0
31	3192.5
32	5948.5
33	9813.5
34	14652.5
35	21591.0
36	32431.0
37	41573.5
38	43805.0
39	43075.0
40	39944.0
41	35907.0
42	32994.0
43	30241.0
44	28516.5
45	27189.0
46	25340.0
47	23171.0
48	21963.0
49	23582.5
50	26269.0
51	25882.5
52	23918.5
53	24108.5
54	26384.0
55	26863.0
56	22560.5
57	19061.5
58	18741.5
59	18339.0
60	15318.5
61	11223.5
62	8804.0
63	5920.5
64	3372.0
65	2160.5
66	1702.5
67	1463.0
68	1063.5
69	667.5
70	417.0
71	294.0
72	214.5
73	173.5
74	124.5
75	72.5
76	50.5
77	33.0
78	17.5
79	11.5
80	4.0
81	2.0
82	2.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4725353016849647
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.923133082757874E-4
22-23	8.181884552350215E-4
24-25	3.146878673980852E-4
26-27	0.0011328763226331068
28-29	0.0030839411005012347
30-31	3.776254408777022E-4
32-33	1.2587514695923407E-4
34-35	0.0039021295557362563
36-37	8.181884552350215E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.00742663367059481
46-47	0.07363696097115192
48-49	6.293757347961704E-4
50-51	0.0
52-53	6.293757347961704E-5
54-55	0.0
56-57	0.006671382788839407
58-59	0.03971360886563835
60-61	0.02995828497629771
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.888127204388511E-4
78-79	0.005412631319247066
80-81	0.004846193157930512
82-83	6.293757347961704E-4
84-85	8.811260287146385E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	6.293757347961704E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06551801399228134
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	794438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.385668531889124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.25130730608936	23.742124389118022
2	16.010119809151433	11.65077825063232
3	6.746079488363918	7.36381836460157
4	3.479946660021313	5.064807427207605
5	2.1216171059494573	3.859822838433142
6	1.3164469873398845	2.8739882232691842
7	0.9114459331036298	2.3214498724652937
8	0.6752993501016739	1.965697465007972
9	0.5190076187967945	1.6995995264758708
>10	2.5696580060265033	16.81489759944264
>50	0.20542497330062254	5.197756636875318
>100	0.17805802422594558	12.977183661257346
>500	0.012470990023605568	3.0837134142025784
>1k	0.003117747505901392	1.3843623310111146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	1999	0.25162441877150893	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1309	0.1647705673696374	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1195	0.15042080061628474	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1140	0.14349766753352686	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1124	0.1414836651821791	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1081	0.13607103386293204	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1059	0.1333017806298289	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1059	0.1333017806298289	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1017	0.12801502445754107	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	972	0.12235064284437551	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	948	0.11932963931735391	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	865	0.10888200211973748	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	859	0.10812675123798207	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	849	0.10686799976838972	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	835	0.10510574771096044	RNA PCR Primer, Index 46 (95% over 21bp)
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	833	0.10485399741704199	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	822	0.1034693708004904	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.5175029391846814E-4	0.0
3	0.0	0.0	0.0	2.5175029391846814E-4	0.0
4	0.0	0.0	0.0	2.5175029391846814E-4	0.0
5	0.0	0.0	0.0	2.5175029391846814E-4	0.0
6	0.0	0.0	0.0	2.5175029391846814E-4	0.0
7	0.0	0.0	0.0	2.5175029391846814E-4	0.0
8	0.0	0.0	0.0	2.5175029391846814E-4	0.0
9	0.0	0.0	0.0	3.776254408777022E-4	0.0
10-11	0.0	0.0	0.0	3.776254408777022E-4	0.0
12-13	0.0	0.0	0.0	3.776254408777022E-4	0.0
14-15	0.0	0.0	0.0	5.035005878369363E-4	0.0
16-17	0.0	0.0	0.0	7.552508817554044E-4	0.0
18-19	0.0	0.0	0.0	0.001384626616551575	0.0
20-21	0.0	0.0	0.0	0.0016363769104700428	0.0
22-23	0.0	0.0	0.0	0.0029580659535420007	0.0
24-25	0.0	0.0	0.0	0.004909130731410129	0.0
26-27	0.0	0.0	0.0	0.006860195509278257	0.0
28-29	0.0	0.0	0.0	0.013531578298117661	0.0
30-31	0.0	0.0	0.0	0.03291635092983971	0.0
32-33	0.0	0.0	0.0	0.0716858961932838	0.0
34-35	0.0	0.0	0.0	0.12090307865434433	0.0
36-37	0.0	0.0	0.0	0.19535822808073128	0.0
38-39	0.0	0.0	0.0	0.31556899342679984	0.0
40-41	0.0	0.0	0.0	0.46045128757687825	0.0
42-43	0.0	0.0	0.0	0.6016202648916593	0.0
44-45	0.0	0.0	0.0	0.7529221915366586	0.0
46-47	0.0	0.0	0.0	0.9283292088243513	0.0
48-49	0.0	0.0	0.0	1.1157573026466507	0.0
50-51	0.0	0.0	0.0	1.312626032490893	0.0
52-53	0.0	0.0	0.0	1.5055296952059192	0.0
54-55	0.0	0.0	0.0	1.6851535299167462	0.0
56-57	0.0	0.0	0.0	1.8930363351199213	0.0
58-59	0.0	0.0	0.0	2.132324989489425	0.0
60-61	0.0	0.0	0.0	2.3996208640573586	0.0
62-63	0.0	0.0	0.0	2.6825252568482374	0.0
64-65	0.0	0.0	0.0	2.935597239809778	0.0
66-67	0.0	0.0	0.0	3.21787225686586	0.0
68-69	0.0	0.0	0.0	3.545210576533348	0.0
70-71	0.0	0.0	0.0	3.9081715627902995	0.0
72-73	0.0	0.0	0.0	4.273650051986436	0.0
74-75	0.0	0.0	0.0	4.633904722583763	0.0
76-77	0.0	0.0	0.0	5.005299343686984	0.0
78-79	0.0	0.0	0.0	5.423897144899916	0.0
80-81	0.0	0.0	0.0	5.90480314385767	0.0
82-83	0.0	0.0	0.0	6.407485543239371	0.0
84-85	0.0	0.0	0.0	6.900286743584774	0.0
86-87	0.0	0.0	0.0	7.404164956862587	0.0
88-89	0.0	0.0	0.0	7.9693443667095485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	43.935215	1
CGTTATA	45	9.3723426E-4	42.44948	1
GTATCAA	1720	0.0	41.36973	1
CCGTGCG	70	4.314028E-6	40.706707	9
ATCAACG	2110	0.0	33.76149	3
TCAACGC	2175	0.0	32.752525	4
AATGCCG	95	3.493938E-5	29.994415	9
TCTATAC	310	0.0	29.107483	3
GTACATG	4990	0.0	28.040901	1
TATCAAC	2595	0.0	27.634548	2
CAACGCA	2640	0.0	27.163502	5
CGCAGAG	2610	0.0	27.11181	8
AACGCAG	2675	0.0	26.630556	6
CGATTAT	200	7.185008E-10	26.120136	2
ACATGGG	4915	0.0	26.088732	3
ACGCAGA	2760	0.0	25.638342	7
ACCGTGC	130	1.0030466E-5	25.572163	8
TATAATA	155	1.4120069E-6	24.511564	2
TACATGG	5575	0.0	24.27799	2
CATGGGA	2645	0.0	24.239342	4
>>END_MODULE