FastQCFastQC Report
Fri 10 Feb 2017
SRR3224548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764230
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG12490.16343247451683393No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA8010.1048113787734059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC652.592442E-643.8357968
AGGTACG550.002583734534.5327727
TCAACGC8550.032.7657474
GTATCAA9100.031.8748531
CCGTGCG600.00394457731.6757669
ATCAACG8850.031.1185173
TCGCACC4050.029.3524741
TCGGACC350.0083779927.15243578-79
TATCACG700.00840488127.1328932
AATGCGG908.963988E-426.3792027
CGCACCA4650.025.528262
GTACATG20700.025.2686541
CATGGGG12100.024.7223264
CGCAGAG11250.024.4831058
GGCCGTG5450.024.3947127
CAACGCA11500.024.3606195
GGTATCA7100.024.110371
AGGCCGT5350.023.9631636
GCGTGCG1401.7745167E-523.7568249
AAATGCG1201.7143475E-423.7412826
Produced by FastQC (version 0.11.5)