##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224548_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	764230
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3926043206888	34.0	31.0	34.0	31.0	34.0
2	32.55172264894076	34.0	31.0	34.0	31.0	34.0
3	32.69936537429831	34.0	31.0	34.0	31.0	34.0
4	35.96649830548395	37.0	37.0	37.0	35.0	37.0
5	35.98856626931683	37.0	37.0	37.0	35.0	37.0
6	36.08765816573545	37.0	37.0	37.0	35.0	37.0
7	36.058833073812856	37.0	37.0	37.0	35.0	37.0
8	36.091151878361224	37.0	37.0	37.0	35.0	37.0
9	37.84189968988394	39.0	39.0	39.0	37.0	39.0
10-11	37.930585687554796	39.0	39.0	39.0	37.0	39.0
12-13	37.94486672860265	39.0	39.0	39.0	37.0	39.0
14-15	39.4996310011384	41.0	40.0	41.0	38.0	41.0
16-17	39.34523769022415	41.0	40.0	41.0	37.5	41.0
18-19	39.43553380526805	41.0	40.0	41.0	37.0	41.0
20-21	39.50355848370255	41.0	40.0	41.0	38.0	41.0
22-23	39.47216871884119	41.0	40.0	41.0	37.5	41.0
24-25	39.4417760360101	41.0	40.0	41.0	37.0	41.0
26-27	39.306834329979196	41.0	40.0	41.0	37.0	41.0
28-29	39.24393834316894	41.0	40.0	41.0	36.5	41.0
30-31	39.18464794629889	41.0	40.0	41.0	36.0	41.0
32-33	39.016308572026745	41.0	39.5	41.0	36.0	41.0
34-35	39.04531292935373	41.0	39.0	41.0	36.0	41.0
36-37	39.061497847506644	41.0	39.0	41.0	36.0	41.0
38-39	38.97933213823064	41.0	39.0	41.0	35.5	41.0
40-41	38.92850843332505	41.0	39.0	41.0	35.0	41.0
42-43	38.82939625505411	41.0	39.0	41.0	35.0	41.0
44-45	38.71359800060192	41.0	39.0	41.0	35.0	41.0
46-47	38.608081991023646	40.0	39.0	41.0	35.0	41.0
48-49	38.47668568362927	40.0	38.0	41.0	34.5	41.0
50-51	37.27600395168994	39.0	36.5	40.0	32.5	40.5
52-53	37.63361488033708	39.5	37.5	40.5	33.5	40.5
54-55	38.166672991115234	40.0	38.0	41.0	34.0	41.0
56-57	38.095464061866195	40.0	37.0	41.0	34.0	41.0
58-59	38.02128416837863	40.0	37.0	41.0	34.0	41.0
60-61	37.84145087211965	40.0	36.5	41.0	34.0	41.0
62-63	37.60768355076351	40.0	36.0	41.0	33.0	41.0
64-65	37.40382869031574	39.5	35.5	41.0	33.0	41.0
66-67	37.19257815055677	39.0	35.0	41.0	33.0	41.0
68-69	36.92394501655261	39.0	35.0	41.0	32.5	41.0
70-71	36.637210002224464	38.5	35.0	40.5	32.0	41.0
72-73	36.308842233359066	37.0	35.0	40.0	32.0	41.0
74-75	35.982664250291144	37.0	35.0	39.5	32.0	41.0
76-77	35.576735406880125	36.5	35.0	39.0	31.0	41.0
78-79	35.24516506810777	36.0	35.0	39.0	31.0	39.5
80-81	34.84721288093898	35.5	35.0	37.0	31.0	39.0
82-83	34.49895973725187	35.0	35.0	37.0	31.0	39.0
84-85	34.18143883385892	35.0	34.5	37.0	30.0	38.0
86-87	33.876013765489446	35.0	34.0	36.0	30.0	37.0
88-89	33.648985253130604	35.0	34.0	36.0	30.0	37.0
90-91	33.42884275676171	35.0	34.0	35.5	29.5	36.5
92-93	33.24663844654096	35.0	34.0	35.0	29.0	36.0
94-95	33.10053583345328	35.0	34.0	35.0	29.0	36.0
96-97	32.95036049356869	35.0	34.0	35.0	29.0	36.0
98-99	32.87443963204794	35.0	34.0	35.0	29.0	35.5
100-101	31.78612786726509	34.5	32.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	109.0
8	460.0
9	392.0
10	341.0
11	592.0
12	898.0
13	1022.0
14	1127.0
15	1149.0
16	1183.0
17	1312.0
18	1433.0
19	1566.0
20	1834.0
21	2218.0
22	2602.0
23	2688.0
24	2810.0
25	2982.0
26	3160.0
27	3531.0
28	4065.0
29	4615.0
30	5648.0
31	7116.0
32	9206.0
33	12913.0
34	20646.0
35	35534.0
36	73634.0
37	148259.0
38	286489.0
39	122676.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34624674172441	20.64537773718084	13.639348043462022	23.369027477632734
2	14.687201496931552	20.60034282872957	41.43412323515172	23.278332439187153
3	19.493055719119738	25.84104702012232	29.483425060126194	25.18247220063175
4	12.120827499574736	17.24467764939874	40.36821375763841	30.266281093388116
5	12.997003519882758	38.45622391164964	36.05655365531319	12.490218913154418
6	29.170275964042236	38.69607317168915	19.40122737919213	12.732423485076483
7	26.411682346937443	31.84695706789841	23.490441359276655	18.250919225887493
8	23.973672847179515	37.05716865341586	21.50216557842534	17.466992920979287
9	24.949949622495847	16.945291338994807	21.28757049579315	36.8171885427162
10-11	24.172107873284222	26.745613231618755	29.672022820355124	19.4102560747419
12-13	24.710754615757036	24.20501681430983	29.509833427109637	21.574395142823498
14-15	21.612858254590247	26.304914262376304	26.67582545247958	25.406402030553863
16-17	21.7392756215674	29.46365667322304	27.4611921417852	21.335875563424363
18-19	21.70430926615255	28.848353725256143	28.39745402000974	21.04988298858157
20-21	22.205858770319317	27.756668924823032	28.51318403319817	21.524288271659483
22-23	22.126430949426965	28.12042726341254	27.882964131740646	21.870177655419848
24-25	21.811674956500458	28.050350188492562	28.150665793294888	21.987309061712093
26-27	21.86782774819099	28.561166141083184	28.104431911858995	21.466574198866834
28-29	21.844405480025646	28.296651531606976	28.226188451120738	21.63275453724664
30-31	22.04192455150936	27.964225429517292	28.15709930256598	21.836750716407362
32-33	21.778064195333865	27.92699841670701	28.376339583633197	21.918597804325923
34-35	22.2588749460241	27.83821624380095	27.944074427855487	21.95883438231946
36-37	21.791810336647476	27.816427883831985	28.432161047368066	21.959600732152474
38-39	21.807392092332453	28.174287976236435	28.026092816624033	21.992227114807083
40-41	22.104971822609045	27.793251951610653	28.409732568326817	21.692043657453482
42-43	21.94316551328413	27.901737957855353	28.22606341390327	21.929033114957246
44-45	21.978372874879113	27.88199174750992	28.073254263328508	22.06638111428246
46-47	22.165905551993507	27.577888855449277	28.036389568585374	22.21981602397184
48-49	21.66991612472685	27.961215864334036	28.265967051803777	22.102900959135336
50-51	21.893278201588526	28.00354605288984	28.06059694071157	22.04257880481007
52-53	21.845517710636848	27.939429229420465	27.857843842822188	22.3572092171205
54-55	21.91323292726012	27.67334441202256	28.101160645355456	22.312262015361867
56-57	21.983761433076427	27.761472331627914	28.086178244769243	22.16858799052641
58-59	21.940910875539853	27.88051302185578	28.19945033372595	21.97912576887842
60-61	21.92680355636639	27.96538909781447	28.037631470976432	22.070175874842707
62-63	21.932442965661266	27.68826616526493	28.22040559351773	22.158885275556077
64-65	22.148887243706685	27.39791306237575	27.94645259224203	22.506747101675533
66-67	21.673292173395236	27.83733644813946	27.950935259801664	22.538436118663643
68-69	21.823076822422568	27.71502034727765	28.362142287007835	22.09976054329194
70-71	21.94143124452063	27.634285489970296	28.146892951074935	22.277390314434136
72-73	21.877052719730973	27.678905565078576	28.338654593512423	22.10538712167803
74-75	21.915261112492313	27.61603182288055	28.125891420122212	22.342815644504928
76-77	21.814571529513366	27.402679821519698	28.13348075841043	22.64926789055651
78-79	22.031669002775846	27.218093132886008	28.144946086264593	22.605291778073557
80-81	21.716003912239103	27.638764685626448	27.95116771433846	22.69406368779599
82-83	21.75948564168125	27.913247201095015	28.034086319453856	22.29318083776988
84-85	21.985385790904896	27.50599653787881	28.177739673356395	22.3308779978599
86-87	21.668964542015768	27.83348979834998	28.166518057634114	22.331027602000134
88-89	22.097266009255762	27.427674219670884	27.83646712869123	22.638592642382125
90-91	21.879211755623306	27.260052601965377	28.012967300420033	22.847768341991284
92-93	21.845379582975934	27.465305243846345	28.329149221964656	22.360165951213066
94-95	22.061879277180953	27.266006307001817	27.981105164675558	22.691009251141672
96-97	22.18415921908326	27.32423485076482	28.180521570731326	22.311084359420594
98-99	21.65031348772514	27.43297290259603	28.189033458100337	22.727680151578493
100-101	22.181238585857262	27.039555936663373	27.8412133192818	22.937992158197567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	2.0
4	2.0
5	2.0
6	1.5
7	3.0
8	9.5
9	10.5
10	16.5
11	22.0
12	22.5
13	24.5
14	21.0
15	25.5
16	28.0
17	30.5
18	41.0
19	55.0
20	58.0
21	71.5
22	98.5
23	114.0
24	125.5
25	167.5
26	247.5
27	346.0
28	514.0
29	988.5
30	2074.0
31	4235.5
32	7991.0
33	13530.0
34	20236.0
35	27263.0
36	34502.0
37	40241.5
38	43403.5
39	44481.0
40	43227.0
41	40883.5
42	38234.5
43	35213.5
44	31814.5
45	28461.0
46	25780.5
47	23035.5
48	21052.5
49	20360.5
50	20371.0
51	19807.0
52	19038.0
53	19200.5
54	19726.0
55	19478.5
56	16824.5
57	14477.5
58	13511.5
59	12416.0
60	10459.5
61	8094.5
62	6470.0
63	4569.5
64	2854.0
65	1917.0
66	1463.0
67	1309.0
68	1031.0
69	648.5
70	396.5
71	276.0
72	194.5
73	141.0
74	111.5
75	86.0
76	64.0
77	52.0
78	36.5
79	26.5
80	20.5
81	12.0
82	7.0
83	6.5
84	8.0
85	6.5
86	5.0
87	4.0
88	3.0
89	4.0
90	4.5
91	3.5
92	4.5
93	2.5
94	1.0
95	2.5
96	2.5
97	3.0
98	2.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15348782434607383
2	0.0
3	5.234026405663216E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03932062337254492
16-17	0.07798699344438192
18-19	0.026431833348599245
20-21	0.04350784449707549
22-23	0.06967797652539157
24-25	0.01851536841003363
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023749394815696847
38-39	0.004579773104955314
40-41	0.007000510317574552
42-43	0.0037292438140350418
44-45	0.0126925140337333
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.017010585818405453
60-61	0.017730264449184147
62-63	0.031207882443766932
64-65	0.05018122816429609
66-67	0.017991965769467308
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.018188241759679678
80-81	0.061892362246967536
82-83	0.052929092027269274
84-85	0.03238553838504115
86-87	0.06293916752810018
88-89	0.03578765554872224
90-91	0.0
92-93	0.0038600944741766226
94-95	0.0
96-97	0.0
98-99	7.196786307786923E-4
100-101	0.04906899755309265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	764230.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.2629735959031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49982842718956	40.06556104079281
2	15.001202757344135	16.580221437717952
3	5.453925978471964	9.042005020269182
4	2.5173469417766454	5.564643103005206
5	1.3689420250206819	3.7825903491520028
6	0.8319447872452084	2.7585445686468697
7	0.52948770335538	2.0482745478918614
8	0.35561267494895993	1.5721771092858277
9	0.24249315259465576	1.2060803420123185
>10	1.0880880413165424	10.222630814405704
>50	0.06939548097654441	2.703995731409535
>100	0.040539686053219906	3.954997162181843
>500	9.538749652260709E-4	0.33367941121812483
>1k	2.3846874130651772E-4	0.16459936201083647
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1249	0.16343247451683393	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	801	0.1048113787734059	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	1.962759902123706E-4	0.0
24-25	0.0	0.0	0.0	9.81379951061853E-4	0.0
26-27	0.0	0.0	0.0	0.0017664839119113356	0.0
28-29	0.0	0.0	0.0	0.00314041584339793	0.0
30-31	0.0	0.0	0.0	0.010598903471468014	0.0
32-33	0.0	0.0	0.0	0.027086086649307145	0.0
34-35	0.0	0.0	0.0	0.046451984350261044	0.0
36-37	0.0	0.0	0.0	0.08158538659827538	0.0
38-39	0.0	0.0	0.0	0.13601926121717284	0.0
40-41	0.0	0.0	0.0	0.2011828899676799	0.0
42-43	0.0	0.0	0.0	0.27400128233646937	0.0
44-45	0.0	0.0	0.0	0.36343770854323965	0.0
46-47	0.0	0.0	0.0	0.4529395600800806	0.0
48-49	0.0	0.0	0.0	0.5474137367023018	0.0
50-51	0.0	0.0	0.0	0.6512437355246457	0.0
52-53	0.0	0.0	0.0	0.7506248119021761	0.0
54-55	0.0	0.0	0.0	0.8637452075945723	0.0
56-57	0.0	0.0	0.0	0.9987830888606833	0.0
58-59	0.0	0.0	0.0	1.1376156392709	0.0
60-61	0.0	0.0	0.0	1.292019418237965	0.0
62-63	0.0	0.0	0.0	1.4480588304567996	0.0
64-65	0.0	0.0	0.0	1.6169870326995799	0.0
66-67	0.0	0.0	0.0	1.8040380513719692	0.0
68-69	0.0	0.0	0.0	2.0168012247621787	0.0
70-71	0.0	0.0	0.0	2.2399016003035737	0.0
72-73	0.0	0.0	0.0	2.4968922968216374	0.0
74-75	0.0	0.0	0.0	2.7610797796474884	0.0
76-77	0.0	0.0	0.0	3.0325294741111968	0.0
78-79	0.0	0.0	0.0	3.348402967692972	0.0
80-81	0.0	0.0	0.0	3.69267105452547	0.0
82-83	0.0	0.0	0.0	4.075670936759876	6.54253300707902E-5
84-85	0.0	0.0	0.0	4.447679363542389	1.308506601415804E-4
86-87	0.0	0.0	0.0	4.846054198343431	1.308506601415804E-4
88-89	0.0	0.0	0.0	5.2885911309422555	1.308506601415804E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTGC	65	2.592442E-6	43.835796	8
AGGTACG	55	0.0025837345	34.532772	7
TCAACGC	855	0.0	32.765747	4
GTATCAA	910	0.0	31.874853	1
CCGTGCG	60	0.003944577	31.675766	9
ATCAACG	885	0.0	31.118517	3
TCGCACC	405	0.0	29.352474	1
TCGGACC	35	0.00837799	27.152435	78-79
TATCACG	70	0.008404881	27.132893	2
AATGCGG	90	8.963988E-4	26.379202	7
CGCACCA	465	0.0	25.52826	2
GTACATG	2070	0.0	25.268654	1
CATGGGG	1210	0.0	24.722326	4
CGCAGAG	1125	0.0	24.483105	8
GGCCGTG	545	0.0	24.394712	7
CAACGCA	1150	0.0	24.360619	5
GGTATCA	710	0.0	24.11037	1
AGGCCGT	535	0.0	23.963163	6
GCGTGCG	140	1.7745167E-5	23.756824	9
AAATGCG	120	1.7143475E-4	23.741282	6
>>END_MODULE