FastQCFastQC Report
Fri 10 Feb 2017
SRR3224548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764230
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG7820.10232521623071589No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.032.2203981
GTATAGG3100.030.7812731
GTATCGC350.00836414727.1616494-95
AGCGTCC700.00839903827.136768
TCCGCGC700.00839903827.136768
ATCAACG9050.026.23724
TCAACGC9350.025.3953675
GTATCAA9250.025.2739181
AGGCCGT3850.024.6697856
TCGCACC2602.3646862E-1123.8554861
GGGCGGT1000.001655302223.7446676
GTATTAC1852.4330075E-723.2107431
GTAGTAT2703.8198777E-1122.9719491
CGCACCA2955.456968E-1222.537312
CGATTAT1100.00280345221.6868061
GGCCGTG4400.021.5860617
TTATACT5000.020.8953084
AAGGCCG4100.020.8489765
GTACATG21250.020.6560441
CGGTGCG704.85367E-420.35656730-31
Produced by FastQC (version 0.11.5)