##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	764230
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19158237703309	34.0	33.0	34.0	31.0	34.0
2	33.32075945723146	34.0	34.0	34.0	31.0	34.0
3	33.44188791332452	34.0	34.0	34.0	31.0	34.0
4	36.63385106577863	37.0	37.0	37.0	35.0	37.0
5	36.672069926592776	37.0	37.0	37.0	35.0	37.0
6	36.71739528675922	37.0	37.0	37.0	36.0	37.0
7	36.70078902948065	37.0	37.0	37.0	36.0	37.0
8	36.687023540033756	37.0	37.0	37.0	36.0	37.0
9	38.544237991180665	39.0	39.0	39.0	38.0	39.0
10-11	38.57483807230807	39.0	39.0	39.0	37.5	39.0
12-13	38.58137602554205	39.0	39.0	39.0	38.0	39.0
14-15	40.219995289376236	41.0	40.0	41.0	39.0	41.0
16-17	40.233258966541484	41.0	40.0	41.0	39.0	41.0
18-19	40.22682831084883	41.0	40.0	41.0	39.0	41.0
20-21	40.23288080813368	41.0	40.0	41.0	39.0	41.0
22-23	40.17309579576829	41.0	40.0	41.0	39.0	41.0
24-25	40.15762859348625	41.0	40.0	41.0	38.0	41.0
26-27	40.11307721497455	41.0	40.0	41.0	38.0	41.0
28-29	40.08712298653546	41.0	40.0	41.0	38.0	41.0
30-31	40.04008544548107	41.0	40.0	41.0	38.0	41.0
32-33	40.0151583947241	41.0	40.0	41.0	38.0	41.0
34-35	39.95355979220915	41.0	40.0	41.0	38.0	41.0
36-37	39.87843973672847	41.0	40.0	41.0	38.0	41.0
38-39	39.817543148005186	41.0	40.0	41.0	38.0	41.0
40-41	39.724933593289975	41.0	40.0	41.0	37.5	41.0
42-43	39.67507883752273	41.0	40.0	41.0	37.0	41.0
44-45	39.513279379244466	41.0	40.0	41.0	37.0	41.0
46-47	39.35735119008675	41.0	39.5	41.0	36.0	41.0
48-49	39.32322926344163	41.0	39.0	41.0	36.0	41.0
50-51	39.24266385773916	41.0	39.0	41.0	35.0	41.0
52-53	39.088148855710976	41.0	39.0	41.0	35.0	41.0
54-55	38.95843986757913	40.5	38.5	41.0	35.0	41.0
56-57	38.817301728537224	40.0	38.0	41.0	35.0	41.0
58-59	38.6278175418395	40.0	37.5	41.0	35.0	41.0
60-61	38.45032843515696	40.0	37.0	41.0	35.0	41.0
62-63	38.275753372675766	40.0	36.5	41.0	35.0	41.0
64-65	38.0558215458697	40.0	36.0	41.0	34.5	41.0
66-67	37.80849351634979	39.0	35.5	41.0	34.0	41.0
68-69	37.55809703884956	39.0	35.0	41.0	34.0	41.0
70-71	37.26356528793688	39.0	35.0	41.0	34.0	41.0
72-73	36.94545163105347	37.5	35.0	40.0	34.0	41.0
74-75	36.58893853944493	37.0	35.0	39.5	33.5	41.0
76-77	34.60409824267563	35.0	33.0	38.0	30.5	39.0
78-79	35.8316992266726	36.0	35.0	39.0	33.0	39.5
80-81	35.65612839066773	36.0	35.0	37.5	33.0	39.0
82-83	35.34634468680895	35.5	35.0	37.0	33.0	39.0
84-85	35.037687607133975	35.0	35.0	37.0	33.0	38.0
86-87	34.7581310600212	35.0	35.0	36.0	33.0	37.0
88-89	34.53149313688287	35.0	35.0	36.0	33.0	37.0
90-91	34.34369103542127	35.0	35.0	36.0	33.0	36.5
92-93	34.21023840990278	35.0	35.0	35.0	33.0	36.0
94-95	34.081497062402676	35.0	35.0	35.0	33.0	36.0
96-97	34.008992711618234	35.0	35.0	35.0	33.0	36.0
98-99	33.94926854480981	35.0	35.0	35.0	32.0	36.0
100-101	33.19178977532941	34.5	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	6.0
15	23.0
16	51.0
17	117.0
18	223.0
19	307.0
20	530.0
21	695.0
22	881.0
23	1041.0
24	1113.0
25	1417.0
26	1833.0
27	2660.0
28	3394.0
29	3781.0
30	4244.0
31	4988.0
32	6216.0
33	8280.0
34	13471.0
35	25900.0
36	64637.0
37	151718.0
38	316905.0
39	149779.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57791496695672	20.563740602022694	13.647065938009831	23.211278493010756
2	14.74098111824974	20.686965965743298	41.70446069900422	22.867592217002734
3	19.326642502911426	26.085079099224057	29.593970401580677	24.99430799628384
4	12.013660808918782	17.5227353021996	40.62572785679704	29.83787603208458
5	12.88695811470369	38.32498069952763	36.28855187574421	12.499509310024468
6	29.18270677675569	38.60500111223061	19.469923975766456	12.742368135247242
7	26.34717297148764	31.805869960613954	23.540164610130457	18.306792457767948
8	23.874618897452336	37.2048990487157	21.33546183740497	17.58502021642699
9	24.76741295159834	17.005613493320073	21.403896732659017	36.82307682242257
10-11	24.191473770985176	26.719901076900932	29.684257357078366	19.404367795035526
12-13	24.801695824555434	24.203054054407705	29.477251612734385	21.517998508302476
14-15	21.61423916883661	26.488622535100692	26.68587990526412	25.21125839079858
16-17	21.59035892336077	29.629300079818904	27.539353336037582	21.240987660782746
18-19	21.549140965416168	28.92709001216911	28.534407181084227	20.98936184133049
20-21	22.180313389381396	27.861689947332263	28.520265628577974	21.437731034708367
22-23	22.090904511073063	28.120978331487223	28.006874988141494	21.781242169298217
24-25	21.940114730159063	28.020760820069402	28.17123947303684	21.867884976734693
26-27	21.758639212409765	28.63232289262887	28.11852063158487	21.49051726337649
28-29	21.846551243583185	28.287771704638686	28.328925691210323	21.536751360567806
30-31	21.987132011378893	27.89039048862765	28.339932637375004	21.78254486261845
32-33	21.834896238494114	27.941315980757064	28.501881309058742	21.721906471690076
34-35	22.2657172636203	27.892323928502964	28.013431047782678	21.82852776009406
36-37	21.80896027049873	27.806725306129927	28.479700818352878	21.904613605018465
38-39	21.79474765450192	28.16364183557306	28.087879303351087	21.953731206573938
40-41	22.013006555618073	28.027753425016027	28.28297763762218	21.676262381743715
42-43	21.87208039464559	27.800792955000457	28.431362286222733	21.895764364131217
44-45	21.985292626226077	27.991643269959937	28.146777993769746	21.876286110044237
46-47	22.110944951797794	27.655049773589745	28.01947992425929	22.214525350353167
48-49	21.65265129632089	27.747685386612897	28.412742133978348	22.186921183087865
50-51	21.910615914057285	27.86556403177054	28.153958886722585	22.069861167449588
52-53	21.89753084804313	27.91849312379781	28.024678434502704	22.159297593656362
54-55	21.95876895698939	27.687410857987775	28.203812988236525	22.15000719678631
56-57	22.08135760456286	27.6779562649386	28.125108369281946	22.11557776121659
58-59	22.049376203102327	27.853786536511805	28.19715136786312	21.89968589252275
60-61	21.90301728510442	28.158927932155425	27.957745050109196	21.98030973263096
62-63	21.899624458605395	27.787446187666013	28.176792326917287	22.1361370268113
64-65	21.99880925899271	27.647763107964877	27.948130798319877	22.40529683472253
66-67	21.713881946534418	27.91528728262434	27.978030174162228	22.39280059667901
68-69	21.93848710466744	27.6851209714353	28.180586996061397	22.19580492783586
70-71	21.938552529997512	27.817410988838436	27.995171610640774	22.24886487052327
72-73	21.868024024181203	27.789081820917787	28.10063724271489	22.24225691218612
74-75	21.840414534891327	27.51861350640514	28.111824974156995	22.529146984546536
76-77	21.704611326215904	27.37668258253111	28.336868495878843	22.58183759537415
78-79	21.94745806073383	27.302480271091333	27.963262125402643	22.786799542772197
80-81	21.593266028514037	27.70356654475029	27.9389153150088	22.764252111726872
82-83	21.544164647738135	27.755943762672143	28.22164400428931	22.478247585300412
84-85	21.796432807804614	27.485869477744796	28.140397754818835	22.577299959631752
86-87	21.42515996493202	27.72071235099381	28.24051659840624	22.613611085667927
88-89	21.839498580270337	27.335880559517424	27.882574617588947	22.94204624262329
90-91	21.705769205605645	27.358648574382055	27.86111510932573	23.074467110686573
92-93	21.669458147416353	27.375004906899758	28.19812098452037	22.757415961163524
94-95	21.682804914750793	27.238527668372086	27.991049814846313	23.087617602030804
96-97	21.78944820276618	27.315794983185693	28.094487261688233	22.800269552359893
98-99	21.06656373081402	27.53248367638015	28.219449642123447	23.181502950682386
100-101	21.541018852409646	27.209966453761968	27.924166123275224	23.324848570553158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	3.0
4	2.0
5	0.5
6	1.5
7	6.5
8	10.5
9	9.5
10	7.0
11	12.0
12	16.0
13	14.5
14	15.0
15	18.0
16	21.5
17	27.0
18	33.5
19	39.0
20	40.5
21	51.0
22	65.5
23	85.5
24	113.5
25	150.5
26	213.0
27	299.0
28	494.5
29	975.0
30	1997.5
31	4145.5
32	8070.0
33	13613.5
34	20109.0
35	27363.5
36	34922.0
37	40275.5
38	42888.5
39	44032.0
40	43431.0
41	41360.5
42	38363.5
43	35560.5
44	32410.0
45	28821.0
46	25815.0
47	23064.0
48	21156.5
49	20823.5
50	21138.5
51	20259.0
52	18990.5
53	18972.5
54	19624.5
55	19292.5
56	16614.5
57	14157.5
58	13331.0
59	12486.0
60	10460.0
61	7900.0
62	6229.5
63	4404.5
64	2653.0
65	1724.5
66	1307.0
67	1145.5
68	890.5
69	546.0
70	322.5
71	217.5
72	170.0
73	139.5
74	101.0
75	63.5
76	45.5
77	30.0
78	21.5
79	14.0
80	7.5
81	6.0
82	5.5
83	3.0
84	1.5
85	0.5
86	0.5
87	1.0
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46595920076416786
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.542533007079021E-4
22-23	0.0013739319314865943
24-25	2.617013202831608E-4
26-27	0.0033366918336103007
28-29	0.003532967823822671
30-31	0.0010468052811326433
32-33	1.9627599021237064E-4
34-35	0.0039909451343182025
36-37	0.001570207921698965
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.007262211637857713
46-47	0.074846577600984
48-49	0.001243081271345014
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007916464938565614
58-59	0.04180678591523494
60-61	0.03251638904518273
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.925519804247413E-4
78-79	0.007785614278424034
80-81	0.0058882797063711185
82-83	5.888279706371119E-4
84-85	0.0020281852321944963
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06647213535192285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	764230.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.83014119770073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46923658908028	38.47199096882072
2	15.305220354260808	16.477643454635647
3	5.796168343462694	9.360256810027199
4	2.615325212842971	5.6313330194097535
5	1.445754160934851	3.891257531014319
6	0.8820209294269206	2.8487586702227023
7	0.5699005515712504	2.1474479011809557
8	0.3673094081786533	1.581785384440064
9	0.2742917163895888	1.3288645640350123
>10	1.1537780636531985	10.609953750750382
>50	0.07215985727420662	2.735534156746304
>100	0.047377057315743	4.418843510325667
>500	0.001457755608912478	0.4963302783913306
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	782	0.10232521623071589	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	1.962759902123706E-4	0.0
24-25	0.0	0.0	0.0	9.81379951061853E-4	0.0
26-27	0.0	0.0	0.0	0.0017664839119113356	0.0
28-29	0.0	0.0	0.0	0.00314041584339793	0.0
30-31	0.0	0.0	0.0	0.010860604791751174	0.0
32-33	0.0	0.0	0.0	0.027544063959802675	0.0
34-35	0.0	0.0	0.0	0.0474333643013229	0.0
36-37	0.0	0.0	0.0	0.08276304253954961	0.0
38-39	0.0	0.0	0.0	0.13765489446894258	0.0
40-41	0.0	0.0	0.0	0.20340735119008677	0.0
42-43	0.0	0.0	0.0	0.27687999685958414	0.0
44-45	0.0	0.0	0.0	0.36697067636706227	0.0
46-47	0.0	0.0	0.0	0.4567996545542572	0.0
48-49	0.0	0.0	0.0	0.5519280844771862	0.0
50-51	0.0	0.0	0.0	0.6554309566491763	0.0
52-53	0.0	0.0	0.0	0.7556625623176269	0.0
54-55	0.0	0.0	0.0	0.8685866820198107	0.0
56-57	0.0	0.0	0.0	1.0057835991782578	0.0
58-59	0.0	0.0	0.0	1.1466443348206692	0.0
60-61	0.0	0.0	0.0	1.3028800230297162	0.0
62-63	0.0	0.0	0.0	1.4605550685003206	0.0
64-65	0.0	0.0	0.0	1.6318385826256492	0.0
66-67	0.0	0.0	0.0	1.8210486371903747	0.0
68-69	0.0	0.0	0.0	2.0368213757638407	0.0
70-71	0.0	0.0	0.0	2.2628004658283505	0.0
72-73	0.0	0.0	0.0	2.521753922248538	0.0
74-75	0.0	0.0	0.0	2.788623843607291	0.0
76-77	0.0	0.0	0.0	3.0628868272640437	0.0
78-79	0.0	0.0	0.0	3.3813773340486506	0.0
80-81	0.0	0.0	0.0	3.7293092393651124	0.0
82-83	0.0	0.0	0.0	4.11492613480235	0.0
84-85	0.0	0.0	0.0	4.490140402758332	0.0
86-87	0.0	0.0	0.0	4.892833309344046	0.0
88-89	0.0	0.0	0.0	5.33864150844641	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	770	0.0	32.220398	1
GTATAGG	310	0.0	30.781273	1
GTATCGC	35	0.008364147	27.16164	94-95
AGCGTCC	70	0.008399038	27.13676	8
TCCGCGC	70	0.008399038	27.13676	8
ATCAACG	905	0.0	26.2372	4
TCAACGC	935	0.0	25.395367	5
GTATCAA	925	0.0	25.273918	1
AGGCCGT	385	0.0	24.669785	6
TCGCACC	260	2.3646862E-11	23.855486	1
GGGCGGT	100	0.0016553022	23.744667	6
GTATTAC	185	2.4330075E-7	23.210743	1
GTAGTAT	270	3.8198777E-11	22.971949	1
CGCACCA	295	5.456968E-12	22.53731	2
CGATTAT	110	0.002803452	21.686806	1
GGCCGTG	440	0.0	21.586061	7
TTATACT	500	0.0	20.895308	4
AAGGCCG	410	0.0	20.848976	5
GTACATG	2125	0.0	20.656044	1
CGGTGCG	70	4.85367E-4	20.356567	30-31
>>END_MODULE